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- EMDB-44555: Septin Tetrameric Complex SEPT7/SEPT9 of Ciona intestinalis by Cryo-EM -
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Open data
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Basic information
Entry | ![]() | ||||||||||||
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Title | Septin Tetrameric Complex SEPT7/SEPT9 of Ciona intestinalis by Cryo-EM | ||||||||||||
![]() | Raw map | ||||||||||||
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![]() | Complex / SPA / Cytoskeleton / CELL CYCLE | ||||||||||||
Function / homology | ![]() septin complex / cytoskeleton-dependent cytokinesis / septin ring / cell division site / cleavage furrow / cilium assembly / kinetochore / cilium / microtubule cytoskeleton / molecular adaptor activity ...septin complex / cytoskeleton-dependent cytokinesis / septin ring / cell division site / cleavage furrow / cilium assembly / kinetochore / cilium / microtubule cytoskeleton / molecular adaptor activity / GTPase activity / GTP binding / cytosol Similarity search - Function | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.72 Å | ||||||||||||
![]() | Mendonca DC / Pereira HM / Garratt RC | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural Insights into Ciona intestinalis Septins: complexes suggest a mechanism for nucleotide-dependent interfacial cross-talk Authors: Mendonca DC / Morais ST / Ciol H / Pinto AP / Leonardo DA / Valadares NF / Portugal RV / Klaholz BP / Garratt RC / Araujo AP | ||||||||||||
History |
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Structure visualization
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Downloads & links
-EMDB archive
Map data | ![]() | |||
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Header (meta data) | ![]() | |||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 1 MB | Display | ![]() |
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Full document | ![]() | 1 MB | Display | |
Data in XML | ![]() | 24.6 KB | Display | |
Data in CIF | ![]() | 31.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 9bhwMC ![]() 44552 ![]() 9bhtC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Raw map | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.729 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : Ciona intestinalis Septin Tetrameric Complex SEPT7/SEPT9
Entire | Name: Ciona intestinalis Septin Tetrameric Complex SEPT7/SEPT9 |
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Components |
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-Supramolecule #1: Ciona intestinalis Septin Tetrameric Complex SEPT7/SEPT9
Supramolecule | Name: Ciona intestinalis Septin Tetrameric Complex SEPT7/SEPT9 type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 |
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Source (natural) | Organism: ![]() ![]() |
-Macromolecule #1: CiSeptin-7
Macromolecule | Name: CiSeptin-7 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 48.769039 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MVLLNQDMAQ QPKALDSYVG FANLPNQLYR KSVKQGFEFT LMVVGESGLG KSTLINSLFL SDLYSNEYPG PSLRIKKTVK VETTKVLIK ENGVTLRLTI DDTPGFGDAV DNSNCWQAVI NHIEKKFEDY LNAESMVTRS KLADNRVHCC LYFIAPTGHG L KPLDVEFM ...String: MVLLNQDMAQ QPKALDSYVG FANLPNQLYR KSVKQGFEFT LMVVGESGLG KSTLINSLFL SDLYSNEYPG PSLRIKKTVK VETTKVLIK ENGVTLRLTI DDTPGFGDAV DNSNCWQAVI NHIEKKFEDY LNAESMVTRS KLADNRVHCC LYFIAPTGHG L KPLDVEFM KNLHDKVNII PLIAKADTMT PEECLRFKKQ IMKEIHEHKI QLYEFPECED EEENRLNRKL KSRVPFAVVG SN TVLEIGG RRVRGRQYPW GVAEVENIDH CDFTVLRNML VRTHMQDLKD VTNNVHYENY RSKKLSSVTT TTMDGRSKAQ TKS PLAQME EEKSEHMQKM KKMEMEEVFR MKVHEKKQKL QESEADLSRR HEAMKKKLEA EYAALDEKQR QYEKDKQDFE ALQL KLMED RKSDNRTLGR KKKW UniProtKB: Septin |
-Macromolecule #2: Neuronal-specific septin-3
Macromolecule | Name: Neuronal-specific septin-3 / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 41.559363 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MTTPLEGYVG IDTLTEQIRK KALRQGFEFN VMVVGSAGLG KSTLVNTIFK SKVSRRQPEE DYHTPSTVEI KTISHVIEEK GILLKLSVT DTPGFGDQVD NTNCWQPIMR HVNEQYEKYL NEEISIKRRK RIPDTRVHCC IYFIPPSGHS LRLVDIEVMK R LVEIVNVI ...String: MTTPLEGYVG IDTLTEQIRK KALRQGFEFN VMVVGSAGLG KSTLVNTIFK SKVSRRQPEE DYHTPSTVEI KTISHVIEEK GILLKLSVT DTPGFGDQVD NTNCWQPIMR HVNEQYEKYL NEEISIKRRK RIPDTRVHCC IYFIPPSGHS LRLVDIEVMK R LVEIVNVI PVIAKSDSLT LEERERFKAT IQQQLIEHNI RVYPDLENLD VDDETERQRN LKLKERLPFA IVGSSTTHQV GS KAVLGRK AGWGVIEVEN DAHCEFNHLR NMIIRTNLQD LKEVTAQVHY ELYRHRRLET LKKVPLDSVG STPSSPSYDI TNS EPNHKK VANTPKLGET GKAIAASQKE VVEALSESKI WSHPQFEK UniProtKB: Neuronal-specific septin-3 |
-Macromolecule #3: GUANOSINE-5'-DIPHOSPHATE
Macromolecule | Name: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 3 / Number of copies: 4 / Formula: GDP |
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Molecular weight | Theoretical: 443.201 Da |
Chemical component information | ![]() ChemComp-GDP: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 41.8 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.5 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 2.72 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 529597 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
-Atomic model buiding 1
Initial model | Chain - Source name: AlphaFold / Chain - Initial model type: in silico model |
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Output model | ![]() PDB-9bhw: |