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- EMDB-36332: Atomic structure of wheat ribosome reveals unique features of the... -

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Basic information

Entry
Database: EMDB / ID: EMD-36332
TitleAtomic structure of wheat ribosome reveals unique features of the plant ribosomes
Map data
Sample
  • Complex: The large subunit of wheat Ribosome
    • RNA: x 1 types
    • Protein or peptide: x 32 types
  • Ligand: x 3 types
KeywordsProtein Synthesis Machinery / Eukaryotic Ribosome / Plant / TRANSLATION
Function / homology
Function and homology information


endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation regulator activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / protein kinase C binding / rRNA processing / ribosomal small subunit biogenesis ...endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation regulator activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / protein kinase C binding / rRNA processing / ribosomal small subunit biogenesis / small ribosomal subunit rRNA binding / ribosome binding / ribosomal small subunit assembly / small ribosomal subunit / cytosolic small ribosomal subunit / cytoplasmic translation / rRNA binding / ribosome / structural constituent of ribosome / positive regulation of protein phosphorylation / ribonucleoprotein complex / translation / mRNA binding / nucleolus / RNA binding / zinc ion binding / nucleus / metal ion binding / cytosol
Similarity search - Function
40S ribosomal protein SA, C-terminal domain / 40S ribosomal protein SA C-terminus / : / Ribosomal protein S26e signature. / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S26e / Ribosomal protein S21e, conserved site / Ribosomal protein S26e superfamily / Ribosomal protein S26e ...40S ribosomal protein SA, C-terminal domain / 40S ribosomal protein SA C-terminus / : / Ribosomal protein S26e signature. / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S26e / Ribosomal protein S21e, conserved site / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S21e signature. / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S2, eukaryotic / Small (40S) ribosomal subunit Asc1/RACK1 / 40S Ribosomal protein S10 / Ribosomal protein S10, eukaryotic/archaeal / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S30 / : / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S7e signature. / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal protein S3Ae, conserved site / Ribosomal protein S19e / Ribosomal protein S3Ae signature. / Ribosomal_S19e / Ribosomal protein S27e signature. / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e signature. / 40S ribosomal protein S4, C-terminal domain / 40S ribosomal protein S4 C-terminus / Ribosomal protein S8e, conserved site / Ribosomal protein S8e signature. / Ribosomal protein S19A/S15e / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal S17 / : / Ribosomal protein S6, eukaryotic / Ribosomal S24e conserved site / Ribosomal protein S24e signature. / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S4e, N-terminal / RS4NT (NUC023) domain / 40S ribosomal protein S11, N-terminal / Ribosomal_S17 N-terminal / Ribosomal protein S7e / Ribosomal protein S7e / Ribosomal protein S4, KOW domain / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal family S4e / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S6e signature. / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal protein S24e / Ribosomal protein S24e / Ribosomal protein S27 / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal protein S27 / Ribosomal protein S8e / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein S3Ae / Ribosomal protein S28e conserved site / Ribosomal S3Ae family / Ribosomal protein S28e signature. / Ribosomal S3Ae family / Ribosomal protein S28e / Ribosomal protein S28e / Ribosomal protein S6e / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S6e / Ribosomal protein S6e / Ribosomal protein S13/S15, N-terminal / Ribosomal protein S15P / Ribosomal S13/S15 N-terminal domain / Ribosomal S13/S15 N-terminal domain / Ribosomal protein S4/S9, eukaryotic/archaeal / Ribosomal protein S8e/ribosomal biogenesis NSA2 / Ribosomal protein S8e / Ribosomal protein S14/S29 / Ribosomal protein S2 signature 2. / Ribosomal protein S3, conserved site / Ribosomal protein S3 signature. / KH domain / Type-2 KH domain profile. / K Homology domain, type 2 / Ribosomal protein S3, C-terminal
Similarity search - Domain/homology
40S ribosomal protein S4 / 40S ribosomal protein S29 / 40S ribosomal protein S17 / Uncharacterized protein / Small ribosomal subunit protein uS2 / 40S ribosomal protein S8 / 40S ribosomal protein S16 / 40S ribosomal protein S25 / Small ribosomal subunit protein eS1 / Small ribosomal subunit protein uS10 domain-containing protein ...40S ribosomal protein S4 / 40S ribosomal protein S29 / 40S ribosomal protein S17 / Uncharacterized protein / Small ribosomal subunit protein uS2 / 40S ribosomal protein S8 / 40S ribosomal protein S16 / 40S ribosomal protein S25 / Small ribosomal subunit protein eS1 / Small ribosomal subunit protein uS10 domain-containing protein / 40S ribosomal protein S26 / 40S ribosomal protein S15 / S5 DRBM domain-containing protein / 40S ribosomal protein S24 / Small ribosomal subunit protein uS17 N-terminal domain-containing protein / 40S ribosomal protein S30 / 30S ribosomal protein S11, chloroplastic / 40S ribosomal protein S18 / Small ribosomal subunit protein uS8c / Small ribosomal subunit protein eS32 / 40S ribosomal protein S7 / Plectin/eS10 N-terminal domain-containing protein / 40S ribosomal protein S6 / Small ribosomal subunit protein uS7c / 40S ribosomal protein S28 / 40S ribosomal protein S21 / 30S ribosomal protein S4, chloroplastic / 40S ribosomal protein S23 / 40S ribosomal protein S19 / 40S ribosomal protein S27 / Small ribosomal subunit protein uS15 N-terminal domain-containing protein / 30S ribosomal protein S3, chloroplastic
Similarity search - Component
Biological speciesTriticum aestivum (bread wheat)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.88 Å
AuthorsMishra RK / Sharma P / Hussain T
Funding support United Kingdom, 1 items
OrganizationGrant numberCountry
Wellcome Trust United Kingdom
Citation
Journal: Structure / Year: 2024
Title: Cryo-EM structure of wheat ribosome reveals unique features of the plant ribosomes.
Authors: Rishi Kumar Mishra / Prafful Sharma / Faisal Tarique Khaja / Adwaith B Uday / Tanweer Hussain /
Abstract: Plants being sessile organisms exhibit unique features in ribosomes, which might aid in rapid gene expression and regulation in response to varying environmental conditions. Here, we present high- ...Plants being sessile organisms exhibit unique features in ribosomes, which might aid in rapid gene expression and regulation in response to varying environmental conditions. Here, we present high-resolution structures of the 60S and 80S ribosomes from wheat, a monocot staple crop plant (Triticum aestivum). While plant ribosomes have unique plant-specific rRNA modification (Cm1847) in the peptide exit tunnel (PET), the zinc-finger motif in eL34 is absent, and uL4 is extended, making an exclusive interaction network. We note differences in the eL15-helix 11 (25S) interaction, eL6-ES7 assembly, and certain rRNA chemical modifications between monocot and dicot ribosomes. In eukaryotes, we observe highly conserved rRNA modification (Gm75) in 5.8S rRNA and a flipped base (G1506) in PET. These features are likely involved in sensing or stabilizing nascent chain. Finally, we discuss the importance of the universal conservation of three consecutive rRNA modifications in all ribosomes for their interaction with A-site aminoacyl-tRNA.
#1: Journal: Acta Crystallogr., Sect. D: Biol. Crystallogr. / Year: 2018
Title: Real-space refinement in PHENIX for cryo-EM and crystallography
Authors: Uday AB / Khaja FT
History
DepositionMay 28, 2023-
Header (metadata) releaseMar 27, 2024-
Map releaseMar 27, 2024-
UpdateMay 22, 2024-
Current statusMay 22, 2024Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_36332.png

Downloads & links

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Map

FileDownload / File: emd_36332.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.07 Å/pix.
x 420 pix.
= 449.4 Å		1.07 Å/pix.
x 420 pix.
= 449.4 Å		1.07 Å/pix.
x 420 pix.
= 449.4 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.07 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.35
Minimum - Maximum-1.8460649 - 4.5529623
Average (Standard dev.)-0.004265758 (±0.11922515)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions420420420
Spacing420420420
CellA=B=C: 449.40002 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_36332_half_map_1.map
Projections & Slices
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Half map: #1

Fileemd_36332_half_map_2.map
Projections & Slices
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Sample components

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Entire : The large subunit of wheat Ribosome

EntireName: The large subunit of wheat Ribosome
Components
  • Complex: The large subunit of wheat Ribosome
    • RNA: 18S rRNA
    • Protein or peptide: 40S ribosomal protein SA
    • Protein or peptide: 40S ribosomal protein S3a
    • Protein or peptide: S5 DRBM domain-containing protein
    • Protein or peptide: 40S ribosomal protein S4
    • Protein or peptide: 40S ribosomal protein S6
    • Protein or peptide: 40S ribosomal protein S7
    • Protein or peptide: 40S ribosomal protein S8
    • Protein or peptide: 30S ribosomal protein S4, chloroplastic
    • Protein or peptide: 40S ribosomal protein S11 N-terminal domain-containing protein
    • Protein or peptide: Ribosomal protein S13/S15 N-terminal domain-containing protein
    • Protein or peptide: 40S ribosomal protein S14
    • Protein or peptide: 40S ribosomal protein S17
    • Protein or peptide: 40S ribosomal protein S21
    • Protein or peptide: 30S ribosomal protein S8, chloroplastic
    • Protein or peptide: 40S ribosomal protein S23
    • Protein or peptide: 40S ribosomal protein S24
    • Protein or peptide: 40S ribosomal protein S26
    • Protein or peptide: 40S ribosomal protein S27
    • Protein or peptide: 40S ribosomal protein S30
    • Protein or peptide: 60S ribosomal protein L41
    • Protein or peptide: KH type-2 domain-containing protein
    • Protein or peptide: Ribosomal protein S7 domain-containing protein
    • Protein or peptide: Plectin/S10 N-terminal domain-containing protein
    • Protein or peptide: 40S ribosomal protein S15
    • Protein or peptide: 40S ribosomal protein S16
    • Protein or peptide: 40S ribosomal protein S18
    • Protein or peptide: 40S ribosomal protein S19
    • Protein or peptide: Ribosomal protein S10 domain-containing protein
    • Protein or peptide: 40S ribosomal protein S28
    • Protein or peptide: 40S ribosomal protein S29
    • Protein or peptide: Mitogen-activated protein kinase
    • Protein or peptide: 40S ribosomal protein S25
  • Ligand: POTASSIUM ION
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION

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Supramolecule #1: The large subunit of wheat Ribosome

SupramoleculeName: The large subunit of wheat Ribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#33
Source (natural)Organism: Triticum aestivum (bread wheat)

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Macromolecule #1: 18S rRNA

MacromoleculeName: 18S rRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 584.3755 KDa
SequenceString: UACCUGGUUG AUCCUGCCAG UAGUCAU(A2M)UG CUUGUCU(OMC)AA AGAUUAAGCC AUGCAUGUGC AAGUAUGAAC CA AUUUGAA CUGUGAAACU GCGAAUGGCU CAU(PSU)AAAUCA G(PSU)UAUAGUUU G(PSU)U(OMU)GAUGGU ACGUGCU AC UCGGAUAACC ...String:
UACCUGGUUG AUCCUGCCAG UAGUCAU(A2M)UG CUUGUCU(OMC)AA AGAUUAAGCC AUGCAUGUGC AAGUAUGAAC CA AUUUGAA CUGUGAAACU GCGAAUGGCU CAU(PSU)AAAUCA G(PSU)UAUAGUUU G(PSU)U(OMU)GAUGGU ACGUGCU AC UCGGAUAACC GUAGUAAUUC UAG(A2M)GCUAAU ACGUGCAACA AACCCCGACU UCUGGGAGGG GCGCAUUUA(PSU) UAGAUAAAA GGCUGACGCG GGCUCUGCUC GCUGAUCC(OMG)A UGAUUCA(PSU)GA (PSU)AACUCGACG GAUCGCACG GCCUUCGUGC CGGCGACGCA UCA(PSU)UCAAA(PSU) UUCUGCCCUA UCAACUUUCG AUGGUAGGAU AGGGGCCUAC CA UGGUGGU GACGGG(PSU)GAC GGAGAAUUAG GGUUCGA(PSU)UC CGGAGA(OMG)GGA GCCUGAGAAA CGGCUACCAC A U(OMC)CAAGGA AGGCAGCAGG CGCGC(A2M)AAUU ACCCAA(PSU)CCU GACACGGGGA GGU(A2M)GUGA(OMC)A AU AAAUAAC AAUACCGGGC GCAUUAGUGU CUGGUAAUUG GAAUGAGUAC AAUCUAAAUC CCUUAACGAG G(A2M)UCCAUUG GAGGGCAAGU CUGGUGCCAG CAGCCGCGGU AA(PSU)UCCAGCU CCAAUA(OMG)CGU AUA(PSU)UUAAGU UG(OMU)U GCAGU U(A2M)AAAAGCUC GUAG(PSU)UGGAC CUUGGGCCGG GUCGGCCGGU CCGCCUCACG GCGAGCACCG ACCUACU CG ACCCUUCGGC CGGCAUCGCG CUCCUAGCCU UAAUUGGCCG GGUCGUGUUU CCGGCAUCGU UACU(PSU)UGAAG AAA (PSU)UAGAG UGCUCAAAGC AAGCCAUCGC UCUGGAU(A2M)CA UU(A2M)GCAUGGG A(PSU)AACAUCAU AGGAUUCC G GUCCUAUUGU GUUGGCCUUC GGGAUCGGAG UAAUGAUUAA UAGGGACAGU CGGGGGCAUU CGUAUUUCAU AGUCAGAGG UGAAAU(PSU)CUU GGAUUUAUGA AAGACGAACA ACUGCGAAAG CAU(PSU)(PSU)GCCAA GGAUGUUUUC AUUAAUCA A GA(A2M)CGAAAGU UGGGGGCUCG AAGACGA(PSU)CA GAUACCG(OMU)CC UAGUCUCAAC CA(PSU)AAACGAU GCC GACCAG GGAUCGGCGG AUGUUGCUUA UAGGACUCCG CCGGCACCUU AUGAGAAAUC AAAGUCUUUG GG(PSU)UCCGGGG GGAGUA(PSU)GG UCGCAAGGCU GAAACUUAAA GGAAUUGACG GAAGGGCACC ACCAGGCGUG GAGCCUGCGG CUUAAUU UG ACUCAACACG GGGAAACUUA CCAGGUCCAG ACAUAGCAAG GAUUGACAGA CUGAGAGCUC UUUCUUGAUU CUAUGGGU G GUGGUGCAUG GCCGUUCUUA GUUGGUGGAG CGAUUUGUCU GGUUAAUUCC GUUAACGAAC GAGACCUCAG CCUGCUAAC UAGCUAUGCG GAGCCAUCCC UCCGCAGCUA GCUUCUUAGA GGGACUAUCG CCGUUUAGGC GACGGAAGUU UGAGGCAAUA ACAGGUCUG UGAUGCCCUU AGAUGUUCUG GGCCGCACGC GCGCUACACU GAUGUAUUCA ACGAGUAUAU AGCCUUGGCC G ACAGGCCC GGGUAAUCUU GGGAAAUUUC AUCGUGAUGG GGAUAGAUCA UUGCAAUUGU UGGUCUUCAA CGAGGAAUGC CU AGUAAGC GCGAGUCAUC AGCUCGCGUU GACUACGUCC CUGCCCUUUG UACACACCG(OMC) CCGUCGCUCC UACCGAUUG AAUGGUCCGG UGAAGUGUUC GGAUCGCGGC GACGGGGGCG GUUCGCCGCC CCCGACGUCG CGAGAAGUCC AUUGAACCUU AUCAUUUAG AGGA(A2M)GGAGA AGUCGUAACA AGGUUUCCGU AGGUG(MA6)(MA6)CCU GCGGAAGGAU CAUUG

GENBANK: GENBANK: XR_006452643.1

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Macromolecule #2: 40S ribosomal protein SA

MacromoleculeName: 40S ribosomal protein SA / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 32.926117 KDa
SequenceString: MAAEGGGVRA LSQREQDIQM MLAADVHLGT KNCDFQMERY VYKRRTDGIY IINLGKTWEK LQMAARVIVA IENPQDIIVQ SARPYGQRA VLKFAQYTGA NAIAGRHTPG TFTNQMQTSF SEPRLLILTD PRTDHQPIKE SALGNIPTIA FCDTDSPMRY V DIGIPANN ...String:
MAAEGGGVRA LSQREQDIQM MLAADVHLGT KNCDFQMERY VYKRRTDGIY IINLGKTWEK LQMAARVIVA IENPQDIIVQ SARPYGQRA VLKFAQYTGA NAIAGRHTPG TFTNQMQTSF SEPRLLILTD PRTDHQPIKE SALGNIPTIA FCDTDSPMRY V DIGIPANN KGKQSIGCLF WLLARMVLQM RGTILPGHKW EIMVDLFFYR DPEEAKELEE DEALGAPEYA AVAEYTAPAG DT WGAEWPG AAAPAAAVEA PAGAEWTGAP APAADGWDAV AAPAPTGWEQ GSAPAPAAAP AATPNWE

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #3: 40S ribosomal protein S3a

MacromoleculeName: 40S ribosomal protein S3a / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 30.043955 KDa
SequenceString: MAVGKNKRIS KGRKGSKKKA VDPFTKKQWY DIKAPLLFTS RNVGKTLVSR TQGTKIASEG LKHRVFEVSL ADLQNDEDQA YRKIRLRAE DVQGMNVLTN FWGMDFTTDK LRSLVRKWQT LIEAHVDVKT TDNYMLRMFA IGFTKRRPNQ VKRTCYAQAS Q IRQIRRKM ...String:
MAVGKNKRIS KGRKGSKKKA VDPFTKKQWY DIKAPLLFTS RNVGKTLVSR TQGTKIASEG LKHRVFEVSL ADLQNDEDQA YRKIRLRAE DVQGMNVLTN FWGMDFTTDK LRSLVRKWQT LIEAHVDVKT TDNYMLRMFA IGFTKRRPNQ VKRTCYAQAS Q IRQIRRKM VEIMVNQAAS CDLKELVNKF IPEVIGKEIE KATSSIFPLQ NVYVRKVKIL KAPKFDLGKL MEVHGDYKED VG VKLDRPA DGDEVIPGAE EVAAAE

UniProtKB: Small ribosomal subunit protein eS1

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Macromolecule #4: S5 DRBM domain-containing protein

MacromoleculeName: S5 DRBM domain-containing protein / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 30.482432 KDa
SequenceString: MADRGGERGV GERGGDRGGF GRGFGRGGRG DRGGRRGGRR GPRQEEEKWV PVTKLGRLVK EGRFTKMEEI YLHSLPVKEH QIVETLCPG LKDEVMKITP VQKQTRAGQR TRFKAFVVVG DSNGHVGLGV KCAKEVATAI RGAIILAKLS IVPVRRGYWG N KIGQPHTV ...String:
MADRGGERGV GERGGDRGGF GRGFGRGGRG DRGGRRGGRR GPRQEEEKWV PVTKLGRLVK EGRFTKMEEI YLHSLPVKEH QIVETLCPG LKDEVMKITP VQKQTRAGQR TRFKAFVVVG DSNGHVGLGV KCAKEVATAI RGAIILAKLS IVPVRRGYWG N KIGQPHTV PCKVTGKCGS VTVRMVPAPR GSGIVAARVP KKVLQFAGID DVFTSSRGST KTLGNFVKAT FDCLMKTYGF LT PDFWRET TFTKAPYQEF TDILAKPTKA LMLDAPAEKI EA

UniProtKB: S5 DRBM domain-containing protein

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Macromolecule #5: 40S ribosomal protein S4

MacromoleculeName: 40S ribosomal protein S4 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 30.008111 KDa
SequenceString: MARGLKKHLK RLNAPSHWML DKLGGAFAPK PSSGPHKARE CLPLILILRN RLKYALTYRE VQSILMQRHI MVDGKVRTDK TYPAGFMDI ISIPKTGENY RLLYDTKGRF RLHSVRDEDA KFKLCKVRSV QFGQKGIPYL NTYDGRTIRY PDPLIKANDT I KLDLETNK ...String:
MARGLKKHLK RLNAPSHWML DKLGGAFAPK PSSGPHKARE CLPLILILRN RLKYALTYRE VQSILMQRHI MVDGKVRTDK TYPAGFMDI ISIPKTGENY RLLYDTKGRF RLHSVRDEDA KFKLCKVRSV QFGQKGIPYL NTYDGRTIRY PDPLIKANDT I KLDLETNK IVDFIKFDVG NVVMVTGGRN TGRVGVIKNR EKHKGTFETI HVEDAQGHQF ATRLGNVFTI GKGTKPWVSL PK GKGIKLT IIEEQRKRDA AAQAAAKA

UniProtKB: 40S ribosomal protein S4

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Macromolecule #6: 40S ribosomal protein S6

MacromoleculeName: 40S ribosomal protein S6 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 28.522609 KDa
SequenceString: MKLNIANPTT GCQKKVEIDD DQKLRNLYDK RISQEVVGDL LGEEFKGYIF KIMGGCDKQG FPMKQGVLTS GRVRLLLHRG TPCFRGFGR RNGERRRKSV RGCIVSQDLS VINLVIVKKG ENDLPGLTDT EKPRMRGPKR ASKIKKLFNL GKDDDVRSYV N TYRRTFLN ...String:
MKLNIANPTT GCQKKVEIDD DQKLRNLYDK RISQEVVGDL LGEEFKGYIF KIMGGCDKQG FPMKQGVLTS GRVRLLLHRG TPCFRGFGR RNGERRRKSV RGCIVSQDLS VINLVIVKKG ENDLPGLTDT EKPRMRGPKR ASKIKKLFNL GKDDDVRSYV N TYRRTFLN KKGKKVSKAP KIQRLVTPLT LQRKRARIAD KKKRIAKKKS EAAEYQKLLA QRLKEQRDRR SESMAKRRSK LS AATKAPA ASA

UniProtKB: 40S ribosomal protein S6

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Macromolecule #7: 40S ribosomal protein S7

MacromoleculeName: 40S ribosomal protein S7 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 22.297891 KDa
SequenceString: MYTARKKIQK DKGVEPSEFE DTVAQAFFDL ENGNQELKSD LKDLYINTAI QMDVVGNRKA VVIHVPYRLR KPFRKIHVRL VRELEKKFS GKDVVFVATR RIVRPPKKGS AVQRPRTRTL TAVHDGILED VVYPAEIVGK RVRYRLDGAK VIKIYLDPKE R NNTEYKLE ...String:
MYTARKKIQK DKGVEPSEFE DTVAQAFFDL ENGNQELKSD LKDLYINTAI QMDVVGNRKA VVIHVPYRLR KPFRKIHVRL VRELEKKFS GKDVVFVATR RIVRPPKKGS AVQRPRTRTL TAVHDGILED VVYPAEIVGK RVRYRLDGAK VIKIYLDPKE R NNTEYKLE TFSAVYRRLC GKDVVFEYPV TETA

UniProtKB: 40S ribosomal protein S7

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Macromolecule #8: 40S ribosomal protein S8

MacromoleculeName: 40S ribosomal protein S8 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 25.185838 KDa
SequenceString: GISRDSMHKR RATGGKQKAW RKKRKYELGR QPANTKLSSN KTVRRVRVRG GNVKWRALRL DTGNFSWGSE AVTRKTRLLD VVYNSSNNE LVRTQTLVKN AIVQVDAAPF KQWYLTHYGV EIGRKKKAAA AAKKDAAEGQ ESEVAAAATE EAKKSNNVQR K LEKRQQGR ...String:
GISRDSMHKR RATGGKQKAW RKKRKYELGR QPANTKLSSN KTVRRVRVRG GNVKWRALRL DTGNFSWGSE AVTRKTRLLD VVYNSSNNE LVRTQTLVKN AIVQVDAAPF KQWYLTHYGV EIGRKKKAAA AAKKDAAEGQ ESEVAAAATE EAKKSNNVQR K LEKRQQGR TLDSHIEEQF SGGRLLACIS SRPGQCGRAD GYILEGKELE FYMRKLQKKK GKGATA

UniProtKB: 40S ribosomal protein S8

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Macromolecule #9: 30S ribosomal protein S4, chloroplastic

MacromoleculeName: 30S ribosomal protein S4, chloroplastic / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 22.534111 KDa
SequenceString: MVHVNFYRNY GKTFKKPRRP YEKERLDAEL KLVGEYGLRC KRELWRVQYA LSRIRNAARH LLTLDEKNPR RIFEGAALLR RMNRYGLLA EGQDKLDYVL ALTAENFLAR RLQTLVFKAG MAKSIHHARV LIRQRHIRVG RQIVNVPSFM VRVESEKHID F SLTSPFGG ...String:
MVHVNFYRNY GKTFKKPRRP YEKERLDAEL KLVGEYGLRC KRELWRVQYA LSRIRNAARH LLTLDEKNPR RIFEGAALLR RMNRYGLLA EGQDKLDYVL ALTAENFLAR RLQTLVFKAG MAKSIHHARV LIRQRHIRVG RQIVNVPSFM VRVESEKHID F SLTSPFGG GPAGRVKRKN QKKASGGGDA GGDEEEE

UniProtKB: 30S ribosomal protein S4, chloroplastic

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Macromolecule #10: 40S ribosomal protein S11 N-terminal domain-containing protein

MacromoleculeName: 40S ribosomal protein S11 N-terminal domain-containing protein
type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 18.135344 KDa
SequenceString:
MAEQTEKAFL KQPKVFLCSK KTTKGNNKPG KEGNRFWKSV GLGFKTPKEA IEGTYIDKKC PFTGTVSIRG RIIAGTCHSA KMNRTIIVR RNYLHYVKKY QRYEKRHSNI PAHISPCFRV REGDHVIIGQ CRPLSKTVRF NVLKVVPAGS KSGAVKKAFT G A

UniProtKB: Small ribosomal subunit protein uS17 N-terminal domain-containing protein

+
Macromolecule #11: Ribosomal protein S13/S15 N-terminal domain-containing protein

MacromoleculeName: Ribosomal protein S13/S15 N-terminal domain-containing protein
type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 17.132303 KDa
SequenceString:
MGRMHSRGKG ISSSALPYKR TPPSWVKTAV ADVDELITKA AKKGQMPSQI GVLLRDQHGI PLVKSVTGSK ILRILKAHGL APEIPEDLY FLIKKAVAIR KHLERNRKDK DSKFRLILVE SRIHRLARYY KRTKKLPPTW KYESTTASTL VA

UniProtKB: Small ribosomal subunit protein uS15 N-terminal domain-containing protein

+
Macromolecule #12: 40S ribosomal protein S14

MacromoleculeName: 40S ribosomal protein S14 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 16.398818 KDa
SequenceString:
MSSKRKTREP KEENVTLGPA VREGEHVFGV AHIFASFNDT FIHVTDLSGR ETLVRITGGM KVKADRDESS PYAAMLASQD VATRCKELG ITALHIKLRA TGGNKTKTPG PGAQSALRAL ARSGMKIGRI EDVTPVPTDS TRRKGGRRGR RL

UniProtKB: 30S ribosomal protein S11, chloroplastic

+
Macromolecule #13: 40S ribosomal protein S17

MacromoleculeName: 40S ribosomal protein S17 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 16.47909 KDa
SequenceString:
MGRVRTKTVK KTSRQVIEKY YSRMTLDFHT NKKVLEEVSI LQSKRLRNKV AGFTTHLMRR IQRGPVRGIS LKLQEEERER RMDFVPEKS ALEVDEIRVD KETMDMLAFL GMADLPGVER APEATSAAAP YRQPFNGPRG GNRA

UniProtKB: 40S ribosomal protein S17

+
Macromolecule #14: 40S ribosomal protein S21

MacromoleculeName: 40S ribosomal protein S21 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 9.194349 KDa
SequenceString:
MQNEVGDMVD LYVPRKCSAT NRIITAKDHA SVQINIGHVD ENGLYDGRFT TFALSGFVRA QGDADGSLDR LWQKRKAEIK QL

UniProtKB: 40S ribosomal protein S21

+
Macromolecule #15: 30S ribosomal protein S8, chloroplastic

MacromoleculeName: 30S ribosomal protein S8, chloroplastic / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 14.815363 KDa
SequenceString:
MVRVSVLNDA LKSMYNAEKR GKRQVMIRPS SKVIIKFLIV MQKHGYIGEF EYVDDHRSGK IVVELNGRLN KCGVISPRFD VGVKEIEGW TARLLPSRQF GYIVLTTSAG IMDHEEARRK NVGGKVLGFF Y

UniProtKB: Small ribosomal subunit protein uS8c

+
Macromolecule #16: 40S ribosomal protein S23

MacromoleculeName: 40S ribosomal protein S23 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 15.696476 KDa
SequenceString:
MGKTRGMGAG RKLKTHRRNQ RWADKAYKKS HLGNEWKKPF AGSSHAKGIV LEKIGIEAKQ PNSAIRKCAR VQLVKNGKKI AAFVPNDGC LNFIEENDEV LIAGFGRKGH AVGDIPGVRF KVVKVSGVSL LALFKEKKEK PRS

UniProtKB: 40S ribosomal protein S23

+
Macromolecule #17: 40S ribosomal protein S24

MacromoleculeName: 40S ribosomal protein S24 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 15.739685 KDa
SequenceString:
MADAKTAPAV TLRTRKFMTN RLLSRKQFVL EVIHPGRANV SKADLKERLA KIYEVKDSNC IFVFKFRTHF GGGKSTGFGL IYDNLEAAK KFEPKYRLIR NGLATKVEKS RKQIKERKNR TKKIRGVKKT KAGDAKKK

UniProtKB: 40S ribosomal protein S24

+
Macromolecule #18: 40S ribosomal protein S26

MacromoleculeName: 40S ribosomal protein S26 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 15.319789 KDa
SequenceString:
MTFKRRNGGR NKHGRGHVKY IRCSNCAKCC PKDKAIKRFL VRNIVEQAAV RDVQEACVHD GYVLPKLYAK VHHCVSCAIH AHIVRVRSR ENRRNREPPQ RFRRRDDGPR PGQGGPPRAG GPGGAPAGAG AVPGAPAPRV

UniProtKB: 40S ribosomal protein S26

+
Macromolecule #19: 40S ribosomal protein S27

MacromoleculeName: 40S ribosomal protein S27 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 9.583195 KDa
SequenceString:
MVLQNDIDLL NPPAELEKLK HKKKRLVQSP NSFFMDVKCQ GCFNITTVFS HSQTVVVCPG CQTVLCQPTG GKARLTEGCS FRRKGD

UniProtKB: 40S ribosomal protein S27

+
Macromolecule #20: 40S ribosomal protein S30

MacromoleculeName: 40S ribosomal protein S30 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 6.934164 KDa
SequenceString:
MGKVHGSLAR AGKVRGQTPK VAKQDKKKQP RGRAHKRIQY NRRFVTAVVG FGKKRGPNSS EK

UniProtKB: 40S ribosomal protein S30

+
Macromolecule #21: 60S ribosomal protein L41

MacromoleculeName: 60S ribosomal protein L41 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 3.445437 KDa
SequenceString:
MRAKWKKKRM RRLKRKRRKM RQRSK

UniProtKB: Small ribosomal subunit protein eS32

+
Macromolecule #22: KH type-2 domain-containing protein

MacromoleculeName: KH type-2 domain-containing protein / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 25.39873 KDa
SequenceString: MATQISKKKK FVSDGVFYAE LNEMLTRELA EDGYSGVEVR VTPMRTEIII RATRTQNVLG EKGRRIRELT SVVQKRFNFP ENGVELYAE KVVNRGLCAI AQAESLRYKL LGGLAVRRAC YGVLRFVMES GAKGCEVIVS GKLRAQRAKS MKFKDGYMIS S GQPVNEYI ...String:
MATQISKKKK FVSDGVFYAE LNEMLTRELA EDGYSGVEVR VTPMRTEIII RATRTQNVLG EKGRRIRELT SVVQKRFNFP ENGVELYAE KVVNRGLCAI AQAESLRYKL LGGLAVRRAC YGVLRFVMES GAKGCEVIVS GKLRAQRAKS MKFKDGYMIS S GQPVNEYI DAAVRHVLLR QGVLGIKVKI MLDWDPKGKL GPTTPLPDLV TIHPPKEEDE LRPPALVEV

UniProtKB: 30S ribosomal protein S3, chloroplastic

+
Macromolecule #23: Ribosomal protein S7 domain-containing protein

MacromoleculeName: Ribosomal protein S7 domain-containing protein / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 22.350879 KDa
SequenceString: MAVVEPVPGG DVKLFNRWTF EDVQVSDISL NDYLAVTSMK HYTFLPHSAG RYSAKRFRKA QCPIIERLTN SLMMHGRNNG KKIMAVRII KHTMEIIHLL TDANPIQIIV DAIINSGPRE DATRIGSAGV VRRQAVDISP LRRVNQAIYL LTTGARESAF R NVKTIAEC ...String:
MAVVEPVPGG DVKLFNRWTF EDVQVSDISL NDYLAVTSMK HYTFLPHSAG RYSAKRFRKA QCPIIERLTN SLMMHGRNNG KKIMAVRII KHTMEIIHLL TDANPIQIIV DAIINSGPRE DATRIGSAGV VRRQAVDISP LRRVNQAIYL LTTGARESAF R NVKTIAEC LADELINAAK GSSNSYAIKK KDEIERVAKA NR

UniProtKB: Small ribosomal subunit protein uS7c

+
Macromolecule #24: Plectin/S10 N-terminal domain-containing protein

MacromoleculeName: Plectin/S10 N-terminal domain-containing protein / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 20.463965 KDa
SequenceString:
MIISKKNRNE ICKYLFQEGV LYAKKDYNLA KHPQIDVPNL QVIKLMQSFK SKEYVRETFS WQHYYWYLTN DGIEFLRNFL NLPSEIVPA TLKKSARPPG RPLGSGPPGD RPRGPPRFEG DRPRYGDRDG YRGGPRGAPG DFGGEKGGAP AEFQPSFRSS G GGGFGRGG GGGFGRGGGG GGGFGASPME

UniProtKB: Plectin/eS10 N-terminal domain-containing protein

+
Macromolecule #25: 40S ribosomal protein S15

MacromoleculeName: 40S ribosomal protein S15 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 17.295375 KDa
SequenceString:
MADVDVDPEV AAAGPKKRTF RKFSYRGVDL DALLDMSTED LVQLFPARPR RRFSRGLKRK PMALVKKLRK AKSEAPAGEK PEQVRTHLR NMIIMPEMIG SVVGIYNGKC FNQVEIKPEM IGHYLAEFSL SYKPVKHGRP GIGATHSSRF IPLK

UniProtKB: 40S ribosomal protein S15

+
Macromolecule #26: 40S ribosomal protein S16

MacromoleculeName: 40S ribosomal protein S16 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 16.870992 KDa
SequenceString:
MAAVLTRPTP GTVQCFGRKK TAVAVAYCKP GRGLIKVNGA PIELIRPEML RLKAFEPILL AGRSRFKDID MRIRVRGGGK TSQIYSIRQ AIAKSLVAYY QKYVDEAAKK EVKEIFARYD RTLLVADPRR CEPKKFGGRG ARARFQKSYR

UniProtKB: 40S ribosomal protein S16

+
Macromolecule #27: 40S ribosomal protein S18

MacromoleculeName: 40S ribosomal protein S18 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 17.676605 KDa
SequenceString:
MSLSAGEEFQ HILRVLNTNV DGKQKIMFAL TSIKGVGRRF SNIVCKKADI DMNKRAGELS AEEMDRLMAV VHNPRQFKVP DWFLNRKKD YKDGRFSQVV SNAVDMKLRD DLERLKKIRN HRGLRHYWGV RVRGQHTKTT GRRGKTVGVS KKR

UniProtKB: 40S ribosomal protein S18

+
Macromolecule #28: 40S ribosomal protein S19

MacromoleculeName: 40S ribosomal protein S19 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 17.097604 KDa
SequenceString:
MATVPAPAAE TAASTARTVK DVNPHEFVKA YSAHLKRSGK MELPEWVDIV KTARFKELPP SDADWYYIRA ASMARKIYLR QGIGIGGFQ KIYGGRQRNG SRPPHFCKSS GAVSRNILQE LQKMGIIDVD PKGGRLITSQ GRRDLDQVAG TVPAEF

UniProtKB: 40S ribosomal protein S19

+
Macromolecule #29: Ribosomal protein S10 domain-containing protein

MacromoleculeName: Ribosomal protein S10 domain-containing protein / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 13.036149 KDa
SequenceString:
MKSGKAGFEG PQEAQHRIRI TLSSKSVKNL EKVCSDLVRG AKDKLLRVKG PVRMPTKVLN ITTRKSPCGE GTNTWDRFEM RVHKRVIDL VSSPDVVKQI TSITIEPGVE VEVTISDQ

UniProtKB: Small ribosomal subunit protein uS10 domain-containing protein

+
Macromolecule #30: 40S ribosomal protein S28

MacromoleculeName: 40S ribosomal protein S28 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 7.477787 KDa
SequenceString:
MDTQVKLAVV VKVMGRTGSR GQVTQVRVKF LDDQNRLIMR NVKGPVREGD ILTLLESERE ARRLR

UniProtKB: 40S ribosomal protein S28

+
Macromolecule #31: 40S ribosomal protein S29

MacromoleculeName: 40S ribosomal protein S29 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 6.417492 KDa
SequenceString:
MGHSNVWNSH PKNYGPGSRV CRVCGNSHGL IRKYGLMCCR QCFRSNAKDI GFIKYR

UniProtKB: 40S ribosomal protein S29

+
Macromolecule #32: Mitogen-activated protein kinase

MacromoleculeName: Mitogen-activated protein kinase / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 36.314793 KDa
SequenceString: MANMQESLAL VGTMRGHNDV VTAIAAPIDN SPYIVSGSRD KSLLVWDLTN PVPSLGDGTV GTDYAVPFRR LTGHGHFVQD VVISSDGQF ALSGSWDGEL RLWDLSTGLT TRRFVGHTKD VISVAFSVDN RQIVSASRDN TIKLWNTLGE CKYTIGGDMG G GEGHTGWV ...String:
MANMQESLAL VGTMRGHNDV VTAIAAPIDN SPYIVSGSRD KSLLVWDLTN PVPSLGDGTV GTDYAVPFRR LTGHGHFVQD VVISSDGQF ALSGSWDGEL RLWDLSTGLT TRRFVGHTKD VISVAFSVDN RQIVSASRDN TIKLWNTLGE CKYTIGGDMG G GEGHTGWV SCVRFSPNIQ QPTIVSGSWD KTVKVWNLTN CKLRSTLAGH GGYVNAVAVS PDGSLCASGG KDGVTLLWDL SE GKRLYQL EAGSIIHALC FSPNRYWLCA ATEESVKIWD LESKHVVQDL KPEVQVSKNQ MLYCTSLSWS ADGSTLYTGY TDG TIRVFK IQAGFAGYA

UniProtKB: Uncharacterized protein

+
Macromolecule #33: 40S ribosomal protein S25

MacromoleculeName: 40S ribosomal protein S25 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 10.86064 KDa
SequenceString:
MAPKKEKAPA ASSKPAKSGG GKQKEKVNNA VLFDQATYDK LLTEVPKYKQ ITPSVLSERL RINGSLARRA IKDLMERGLI RMVSIHSSQ QIFTRATNT

UniProtKB: 40S ribosomal protein S25

+
Macromolecule #34: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 34 / Number of copies: 16 / Formula: K
Molecular weightTheoretical: 39.098 Da

+
Macromolecule #35: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 35 / Number of copies: 70 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #36: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 36 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.6
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: CONTINUOUS
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 289 K / Instrument: FEI VITROBOT MARK IV
DetailsThe sample is the small subunit of wheat ribosome

-
Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 44.6 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsCalibrated magnification: 75000 / Illumination mode: OTHER / Imaging mode: OTHER / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.5 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

4v7e

Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.88 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 105563
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE
FSC plot (resolution estimation)

+
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