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- EMDB-36331: Atomic structure of wheat ribosome reveals unique features of the... -

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Basic information

Entry
Database: EMDB / ID: EMD-36331
TitleAtomic structure of wheat ribosome reveals unique features of the plant ribosomes
Map data
Sample
  • Complex: The large subunit of wheat Ribosome
    • RNA: x 3 types
    • Protein or peptide: x 41 types
  • Ligand: x 3 types
KeywordsProtein Synthesis Machinery / Eukaryotic Ribosome / Plant / TRANSLATION / RIBOSOME
Function / homology
Function and homology information


protein-RNA complex assembly / maturation of LSU-rRNA / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / ribosome biogenesis / large ribosomal subunit rRNA binding / 5S rRNA binding / ribosomal large subunit assembly / cytoplasmic translation / cytosolic large ribosomal subunit ...protein-RNA complex assembly / maturation of LSU-rRNA / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / ribosome biogenesis / large ribosomal subunit rRNA binding / 5S rRNA binding / ribosomal large subunit assembly / cytoplasmic translation / cytosolic large ribosomal subunit / negative regulation of translation / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / mRNA binding / RNA binding / nucleus / metal ion binding / cytoplasm
Similarity search - Function
Ribosomal protein L2, archaeal-type / Ribosomal protein L28e / Ribosomal protein L23 / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / metallochaperone-like domain / TRASH domain / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L13e, conserved site ...Ribosomal protein L2, archaeal-type / Ribosomal protein L28e / Ribosomal protein L23 / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / metallochaperone-like domain / TRASH domain / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L44e signature. / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L19, eukaryotic / Ribosomal protein L10e / Ribosomal protein L13e / Ribosomal protein L13e / 60S ribosomal protein L18a/ L20, eukaryotes / : / Ribosomal protein L19/L19e conserved site / Ribosomal protein L19e signature. / Ribosomal protein L44e / Ribosomal protein L24e, conserved site / Ribosomal protein L44 / Ribosomal protein L24e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / : / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L30e signature 1. / Ribosomal protein L6e signature. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal L40e family / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L30e signature 2. / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L30e, conserved site / Ribosomal protein L36e signature. / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / Ribosomal protein L39e, conserved site / Ribosomal protein L39e signature. / Ribosomal protein L34Ae / Ribosomal protein L34e / 60S ribosomal protein L19 / Ribosomal Protein L6, KOW domain / Ribosomal protein L30/YlxQ / Ribosomal protein L7A/L8 / 60S ribosomal protein L35 / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L6e / 60S ribosomal protein L6E / Ribosomal protein L18e / Ribosomal protein L7, eukaryotic / Ribosomal protein L30, N-terminal / Ribosomal L30 N-terminal domain / Ribosomal protein L37ae / Ribosomal L37ae protein family / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal protein L36e / Ribosomal_L19e / Ribosomal protein L19/L19e / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L19/L19e superfamily / Ribosomal protein L19e / Ribosomal protein L14e domain / Ribosomal protein L14 / Ribosomal protein L39e / Ribosomal protein L39e domain superfamily / Ribosomal L39 protein / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal large subunit proteins 60S L5, and 50S L18 / Ribosomal protein L6, conserved site-2 / Ribosomal protein L6 signature 2. / Ribosomal protein L14 / Ribosomal protein L14, KOW motif / Ribosomal protein L15e, conserved site / Ribosomal protein L15e signature. / Ribosomal protein L31e
Similarity search - Domain/homology
Large ribosomal subunit protein uL6 alpha-beta domain-containing protein / 60S ribosomal protein L29 / 60S ribosomal protein L36 / Large ribosomal subunit protein uL23 N-terminal domain-containing protein / 60S ribosomal protein L38 / Ubiquitin-like domain-containing protein / Ribosomal protein L37 / 60S ribosomal protein L27 / 60S ribosomal protein L11 / 60S ribosomal protein L18a ...Large ribosomal subunit protein uL6 alpha-beta domain-containing protein / 60S ribosomal protein L29 / 60S ribosomal protein L36 / Large ribosomal subunit protein uL23 N-terminal domain-containing protein / 60S ribosomal protein L38 / Ubiquitin-like domain-containing protein / Ribosomal protein L37 / 60S ribosomal protein L27 / 60S ribosomal protein L11 / 60S ribosomal protein L18a / Large ribosomal subunit protein uL15/eL18 domain-containing protein / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein uL15/eL18 domain-containing protein / Ribosomal protein L19 / Large ribosomal subunit protein uL18 C-terminal eukaryotes domain-containing protein / Large ribosomal subunit protein eL14 domain-containing protein / Uncharacterized protein / TRASH domain-containing protein / KOW domain-containing protein / Uncharacterized protein / 60S ribosomal protein L13 / 60S ribosomal protein L6 / 60S ribosomal protein L7a / 60S ribosomal protein L34 / 60S ribosomal protein L32 / 60S ribosomal protein L37a, expressed / 60S ribosomal protein L21 / Ribosomal protein L17 / Ribosomal protein L39 / Ribosomal protein L13a / Ribosomal protein L3 / Large ribosomal subunit protein uL29 / Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein / Uncharacterized protein / Large ribosomal subunit protein uL2 C-terminal domain-containing protein / Ribosomal protein L15 / Uncharacterized protein / 60S ribosomal protein L31 / Uncharacterized protein
Similarity search - Component
Biological speciesTriticum aestivum (bread wheat)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.84 Å
AuthorsMishra RK / Sharma P / Hussain T
Funding support United Kingdom, 1 items
OrganizationGrant numberCountry
Wellcome Trust United Kingdom
Citation
Journal: Structure / Year: 2024
Title: Cryo-EM structure of wheat ribosome reveals unique features of the plant ribosomes.
Authors: Rishi Kumar Mishra / Prafful Sharma / Faisal Tarique Khaja / Adwaith B Uday / Tanweer Hussain /
Abstract: Plants being sessile organisms exhibit unique features in ribosomes, which might aid in rapid gene expression and regulation in response to varying environmental conditions. Here, we present high- ...Plants being sessile organisms exhibit unique features in ribosomes, which might aid in rapid gene expression and regulation in response to varying environmental conditions. Here, we present high-resolution structures of the 60S and 80S ribosomes from wheat, a monocot staple crop plant (Triticum aestivum). While plant ribosomes have unique plant-specific rRNA modification (Cm1847) in the peptide exit tunnel (PET), the zinc-finger motif in eL34 is absent, and uL4 is extended, making an exclusive interaction network. We note differences in the eL15-helix 11 (25S) interaction, eL6-ES7 assembly, and certain rRNA chemical modifications between monocot and dicot ribosomes. In eukaryotes, we observe highly conserved rRNA modification (Gm75) in 5.8S rRNA and a flipped base (G1506) in PET. These features are likely involved in sensing or stabilizing nascent chain. Finally, we discuss the importance of the universal conservation of three consecutive rRNA modifications in all ribosomes for their interaction with A-site aminoacyl-tRNA.
#1: Journal: Acta Crystallogr., Sect. D: Biol. Crystallogr. / Year: 2018
Title: Real-space refinement in PHENIX for cryo-EM and crystallography
Authors: Uday AB / Khaja FT
History
DepositionMay 28, 2023-
Header (metadata) releaseMar 27, 2024-
Map releaseMar 27, 2024-
UpdateMay 22, 2024-
Current statusMay 22, 2024Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_36331.png

Downloads & links

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Map

FileDownload / File: emd_36331.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
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AxesZ (Sec.)Y (Row.)X (Col.)
1.07 Å/pix.
x 360 pix.
= 385.2 Å		1.07 Å/pix.
x 360 pix.
= 385.2 Å		1.07 Å/pix.
x 360 pix.
= 385.2 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.07 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.09
Minimum - Maximum-0.34478533 - 0.72139686
Average (Standard dev.)0.00070451264 (±0.025131881)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 385.2 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_36331_half_map_1.map
Projections & Slices
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Half map: #1

Fileemd_36331_half_map_2.map
Projections & Slices
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Sample components

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Entire : The large subunit of wheat Ribosome

EntireName: The large subunit of wheat Ribosome
Components
  • Complex: The large subunit of wheat Ribosome
    • RNA: 25S rRNA
    • RNA: 5S rRNA
    • RNA: 5.8S rRNA
    • Protein or peptide: Ribosomal protein L2 C-terminal domain-containing protein
    • Protein or peptide: Ribosomal protein L3
    • Protein or peptide: 60S ribosomal protein L4 C-terminal domain-containing protein
    • Protein or peptide: Ribosomal protein L5 eukaryotic C-terminal domain-containing protein
    • Protein or peptide: 60S ribosomal protein L6
    • Protein or peptide: 60S ribosomal protein uL30
    • Protein or peptide: 60S ribosomal protein L7a
    • Protein or peptide: Ribosomal protein L6 alpha-beta domain-containing protein
    • Protein or peptide: Ribosomal protein L10e/L16 domain-containing protein
    • Protein or peptide: 60S ribosomal protein L11
    • Protein or peptide: 60S ribosomal protein L13
    • Protein or peptide: Ribosomal protein L14e domain-containing protein
    • Protein or peptide: Ribosomal protein L15
    • Protein or peptide: Ribosomal protein L13a
    • Protein or peptide: 60S ribosomal protein uL22
    • Protein or peptide: Ribosomal protein L18e/L15P domain-containing protein
    • Protein or peptide: Ribosomal protein L19
    • Protein or peptide: 60S ribosomal protein L18a
    • Protein or peptide: 60S ribosomal protein L21
    • Protein or peptide: Genome assembly, chromosome: II
    • Protein or peptide: Ribosomal protein L17
    • Protein or peptide: TRASH domain-containing protein
    • Protein or peptide: Genome assembly, chromosome: II
    • Protein or peptide: KOW domain-containing protein
    • Protein or peptide: 60S ribosomal protein L27
    • Protein or peptide: Ribosomal protein L18e/L15P domain-containing protein
    • Protein or peptide: 60S ribosomal protein L29
    • Protein or peptide: Ribosomal protein L7Ae/L30e/S12e/Gadd45 domain-containing protein
    • Protein or peptide: 60S ribosomal protein L31
    • Protein or peptide: 60S ribosomal protein eL32
    • Protein or peptide: 60S ribosomal protein eL33
    • Protein or peptide: 60S ribosomal protein L34
    • Protein or peptide: 60S ribosomal protein L35
    • Protein or peptide: 60S ribosomal protein L36
    • Protein or peptide: Ribosomal protein L37
    • Protein or peptide: 60S ribosomal protein L38
    • Protein or peptide: Ribosomal protein L39
    • Protein or peptide: Ubiquitin-like domain-containing protein
    • Protein or peptide: Genome assembly, chromosome: II
    • Protein or peptide: 60S ribosomal protein L37a, expressed
    • Protein or peptide: Ribosomal L28e/Mak16 domain-containing protein
  • Ligand: POTASSIUM ION
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION

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Supramolecule #1: The large subunit of wheat Ribosome

SupramoleculeName: The large subunit of wheat Ribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#44
Source (natural)Organism: Triticum aestivum (bread wheat)

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Macromolecule #1: 25S rRNA

MacromoleculeName: 25S rRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 1.098269625 MDa
SequenceString: GCGACCCCAG GUCAGGCGGG ACUACCCGCU GAGU(PSU)UAAGC AUAUAAA(OMU)AA GCGGAGGAGA AGAAACU(PSU) A CAAGGAUUCC CCUAGUAACG GCGAGCGAAC CGGGAGCAGC CCAGCUUGAG AAUCGGGCGG CUGUGCCGUC CGAA(OMU) UGUA GUCUGGAGAG ...String:
GCGACCCCAG GUCAGGCGGG ACUACCCGCU GAGU(PSU)UAAGC AUAUAAA(OMU)AA GCGGAGGAGA AGAAACU(PSU) A CAAGGAUUCC CCUAGUAACG GCGAGCGAAC CGGGAGCAGC CCAGCUUGAG AAUCGGGCGG CUGUGCCGUC CGAA(OMU) UGUA GUCUGGAGAG GCGUCCUCAG CGACGGACCG GGCCCAAGUC CCCUGGAAAG GGGCGCCUGG GAGGGUGAGA GCCCCG UCC GGCCCGGACC CUGUCGCCCC ACGAGGCGCC GUCAACGAGU CGGGUUGUUU GGGAAUGCAG CCCAAAUCGG GCGGUAG AC UCCGUCCAAG GCUAAAUACA GGCGAGAGAC CGAUAGCGAA CAAGUACCGC GAGGGAAAGA UG(A2M)AAAGGAC U (PSU)UGAAAAG AGAGUCAAAG AGUGCUUGAA AUUGCCGGGA GGGAAGCGGA UGGGGGCCGG CGAUGCGCCC CGGCCGUA U GCGGAACGGC UCUUGCUGGU CCGCCGCUCG GCUCGGGGUG UGGACUGUUG UCGGCCGCGC CGGCGGCCAA AGCCCGGGG GCCCUAGGUG CCCCCGGUGG CCGUCGUCGG CACGGCCGGU ACCCGCGCGC CUAAAGGCGU GUCCCUCGGG GCACUGCGCU GCAACGGCC UGCGGGCUCC CCAUCCGACC CGUCUUG(1MA)AA C(A2M)CGGACCAA GGAGU(OMC)(OMU)GAC AUGCG UGCG AGUCGACGGG UUCUGAAACC UGGGAUGCGC AAGGAAGCUG ACGAGCGGGA GGCCCUCACG GGCCGCACCG CUGGCC GAC CCUGAUCUUC UGUGAAGGGU UCGAGUUGGA GCACGCC(OMU)GU CGGGACCC(OMG)A (A2M)AGAUGGUGA (A2M) C(PSU)AUGCCU GAGCGGGGCG AAGCCAGAGG AAACUCUGGU GGAGGCUCGA AGCGAUACUG (A2M)CGUGCAAA(PSU) CGUUCGUCU GACUUGGGUA UAG(OMG)GGCGAA AGACUAAUCG AACCAUCUAG U(A2M)GCUGGUUC CCUCCGAAGU UUC CC(PSU)CAG GAUAGCUGGA GCCCAUUACG AGUUCUA(PSU)CA GGUAAAGCCA A(PSU)GAUUAGAG GCAUUGGGGA CG CAACGUC CUCGACCUAU (PSU)CUCAAACUU (PSU)AAA(OMU)AGGUA GGAUGGCGCG GCUGCUUCGG UGAGCCGUGC C AUGGAAUC GGGUGCUCCA AGUGGGCCAU (PSU)(PSU)(PSU)UGGUAAG C(A2M)GAACUGGC GAUGCGGGAU GAACCG GAA GCCGGGUUAC GGUGCCCAAC UGCGCGCUAA CCUAGAACCC ACAAAGGGUG UUGGUCGAUU AAGACAGCAG GACGGUG GU CAUGGAAGUC GAAAUCCGCU AAGGAGUGUG UAACAACUCA CCUGCCGAAU CAACUAGCCC CGAAAAUGGA UGGCGCUG A AGCGCGCGAC CCACACCCGG CCAUCUGGGC GAGCGCCAUG CCCCGAUG(A2M)G UAGGAGGGCG CGGCGGCCGC UGCAA AACC CGGGGCGCGA GCCCGGGCGG AGCGGCCGUC GGUGCAGAU(OMC) UUGGUGGUAG U(A2M)(OMG)CAAAUAU UCAA A(PSU)GAG AA(OMC)U(PSU)UGAAG GCCGAAGAGG AGAAAGGUUC CAUGUGAACG G(OMC)ACUUGCAC AUGGGUAAG (OMC)CGAUCCUAA GGGACGGGGU AACCCCGGCA GAUAGCGCGA UCACGCGCAU CCCCCGAAAG GGAAUCGGGU UAAGAU UUC CCGAGCCGGG AUGUGGCGGU UGACGGCGAC GUUAGGAAGU CCGGAGACGC CGGCGGGGGC CUCGGGAAGA GUUAUCU UU UCUGCUUAAC GGCCUGCCAA CCCUGGAAAC GGUUCAGCCG GAGGUAGGGU CCAGUGGCCG GAAGAGCACC GCACGUCG C GCGGUGUCCG GUGCGCCCCC GGCGGCCCAU GAAAAUCCGG AGGACCGAGU ACCGUUCACG CCCGGUCGUA CUCAUAA (OMC)C G(OMC)AUCA(OMG)GUC U(OMC)CAAGGUGA AC(A2M)GCCUCUG GCCAAUGGAA CAA(OMU)GUAGGC AAGG GAAG(PSU) CGGCAAAACG GAUCCGUAAC UUCGGGAAAA GGAUUGGCUC UGAGGACUGG GCUCGGGGGU CCCGGCCCCG A ACCCGUCG GCUGUCGGCG GAUUGCUCGA GCUGCUCACG CGGCGAGAGC GGGUCGCCGC GUGCCGGCCG GGGGACGGAC CG GGAAUCG CCCCUUCGGG GGCUUUCCCC GAGCAUGAAA CAGUCGACUC AGAACUGG(OMU)A CGGACAAG(OMG)(OMG) G (A2M)AUCCGAC (PSU)G(PSU)UUAAUUA AAACAAAGCA UUGCGAUGGU CCUCGCGGAU GCUGACGCAA UGUGAUU (PSU)C UGCC(OMC)AGUGC UCUGAAUG(PSU)C AAAGUGA(A2M)GA AA(PSU)UCAACCA AGC(OMG)CGGGUA AACG GCGGG AGUAACUAUG ACUCUCUUAA GGUAGC(5MC)AA(A2M) UGCCUC(OMG)UCA U(OMC)UAAUUAGU GACGCGCAU GAA(PSU)GGA(PSU)UA (A2M)CGAGAUUCC CACUGU(OMC)CCU GUCUAC(OMU)A(PSU)C CAGCGAAACC ACAG (OMC)CAAG GGAACGGGCU UGGCGGAAUC A(OMG)CGG(OMG)GAAA GAAGACCCU(OMG) (OMU)UGAGC(PSU)UGA C (OMU)CUAGUCC GAC(PSU)UUGUGA AAUGACUUGA GAGGUGUAGG AUAAGUGGGA GCCCUCACGG GCGCAAGUGA AAUA CCACU ACUUUUAACG UUAUUUUACU UA(PSU)UCCGUGG GUCGGAAGCG GGGCAUGUCC CCUCCUUUUG GCUCCAAGGC C CGGUCUUA CCGGGCCGAU CCGGGCGGAA GACAUUGUCA GGUGGGGAGU U(PSU)GGCUG(OMG)GG CGGCACAUCU GUUA AAAG(A2M) UAACGCAGG(OMU) (OMG)UCCUAAGAU GAGCUCAACG AGAACAGAAA U(OMC)UCGUGUGG AACAAAAGG GUAAAAGCUC GU(PSU)UGAU(OMU)CU GAUUUCCAG(OMU) ACGAA(OMU)ACGA ACCG(PSU)GAAAG CGUGGCCUAU CGAUCCUUU AGAUCUUCGG AGUUUGAAGC UA(OMG)A(OMG)GUGUC AGAAAAGUUA CCACAG(OMG)GAU AACUGGC (PSU)U GUGGCAGC(OMC)A AGCGUUCAUA GCGACG(PSU)UGC UUUUUGA(PSU)CC UU(5MC)GAUGUCG G(OMC) (PSU)CU(OMU)CCU AUCAUUGUGA AGCAGAAUUC ACCA(A2M)GUGUU (OMG)GAU(OMU)(OMG)(PSU)UCA CCCAC CA(A2M)U AGGGAACG(PSU)G (A2M)G(OMC)UGGG(UR3)U(PSU) AGA(OMC)CGUCGU GAGACAGGU(PSU) AGUU UUACC CUACUGA(PSU)GA CAGUGUCGCG AUAGUAAUUC AACCUAGUAC GAGAGGAACC GUUGAUUCAC ACAAUUGGUC A UCGCGCUU GGUUGAAAAG CCAGUGGCGC GAAGCUACCG UGUGCCGGAU U(A2M)UGAC(PSU)GAA CGCCUCUAAG UCAG AAUCC AAGCUAGCAU GCGACGCCUG CGCCCGCCGC CCGCCCCGAC CCACGUUAGG GGCGCUUGCG CCCCCAAGGG CCCGU GCCA UUGGCUAAGC CGGUCCGGCC GAAGUGCCGC GGCCGGCCGC CUCGAAGCUC CCUUCCCAAC GGGCGGUGGG CUGAAU CCU UU(OMG)CAGACGA C(OMU)UAAAUACG CGACGGGGCA UUGUAAGUGG CAGAGUGGCC UUGCUGCCAC GAUCCACUG AGAUCCAGCC CCAUGUCGCA CGGAUUCGU

GENBANK: GENBANK: XR_006476295.1

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Macromolecule #2: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 38.411777 KDa
SequenceString:
GGAUGCGAUC AUACCAGCAC UAAGGCAUCG GAUCCCGUCA GAACUCCGAA GUUAAGCGUG CUUGGGCGAG AGUAGUACAA GGAUGGGUG ACCUCUUGGG AAGUCCUCGU GUUGCAUUCC

GENBANK: GENBANK: X06094.1

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Macromolecule #3: 5.8S rRNA

MacromoleculeName: 5.8S rRNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 50.953305 KDa
SequenceString:
CGACUCUCGG CAACGGA(PSU)AU CUCGGCUCUC GCAUCGAUGA AG(A2M)ACGUAGC GAAAUGCGAU ACCUGGUGUG AA U(PSU)(OMG)CAGA AUCCGUGAAC CAUCGAGUCU UUGAACGCAA GUUGCGCCCG AGGCCACUCG GCCGAGGGCA CGCCU GCCU GGGCGUCACG

GENBANK: GENBANK: AF438188.1

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Macromolecule #4: Ribosomal protein L2 C-terminal domain-containing protein

MacromoleculeName: Ribosomal protein L2 C-terminal domain-containing protein
type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 28.251521 KDa
SequenceString: MGRVIRAQRK GAGSVFKSHT HHRKGPARFR SLDFGERNGY LKGVVTDVIH DPGRGAPLAK VTFRHPFRYK HQKELFVAAE GMYTGQFVY CGRRATLSVG NVLPLRSVPE GGVICNVEHH VGDRGVFARA SGDYAIVISH NPDNGTSRIK LPSGAKKIVP S SCRAMIGQ ...String:
MGRVIRAQRK GAGSVFKSHT HHRKGPARFR SLDFGERNGY LKGVVTDVIH DPGRGAPLAK VTFRHPFRYK HQKELFVAAE GMYTGQFVY CGRRATLSVG NVLPLRSVPE GGVICNVEHH VGDRGVFARA SGDYAIVISH NPDNGTSRIK LPSGAKKIVP S SCRAMIGQ VAGGGRTEKP MLKAGNAYHK YRVKRNSWPK VRGVAMNPVE HPHGGGNHQH IGHASTVRRD APPGQKVGLI AA RRTGRLR GQAAASAAKA DKAT

UniProtKB: Large ribosomal subunit protein uL2 C-terminal domain-containing protein

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Macromolecule #5: Ribosomal protein L3

MacromoleculeName: Ribosomal protein L3 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 44.262797 KDa
SequenceString: MSHRKFEHPR HGSLGFLPRK RCSRHRGKVK SFPKDDQQKP CHLTAFLGYK AGMTHIVREV EKPGSKLHKK ETCEAVTIIE TPPLVIVGL VAYVKTPRGL RTLNSVWAQH LSEDVRRRFY KNWCKSKKKA FTKYALKYDS DAGKKEIQLQ LEKMKKYATI V RVIAHTQI ...String:
MSHRKFEHPR HGSLGFLPRK RCSRHRGKVK SFPKDDQQKP CHLTAFLGYK AGMTHIVREV EKPGSKLHKK ETCEAVTIIE TPPLVIVGL VAYVKTPRGL RTLNSVWAQH LSEDVRRRFY KNWCKSKKKA FTKYALKYDS DAGKKEIQLQ LEKMKKYATI V RVIAHTQI RKMKGLKQKK AHLMEIQVNG GTIADKVDYG YKFFEKEVPV EAVFQKDEMV DIIGVTKGKG YEGVVTRWGV TR LPRKT(HIC)R GLRKVACIGA WHPARVSYTV ARAGQNGYHH RTEMNKKIYK MGKSGQESHE ACTEFDRTEK DITPMGGFP HYGVVKGDYL MIKGCCVGPK KRVVTLRQSL LKQTSRLALE EIKLKFIDTS SKFGHGRFQT TDEKQKFYG

UniProtKB: Ribosomal protein L3

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Macromolecule #6: 60S ribosomal protein L4 C-terminal domain-containing protein

MacromoleculeName: 60S ribosomal protein L4 C-terminal domain-containing protein
type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 44.822043 KDa
SequenceString: MATQTRPLVS VKALEGDMAT DNSSSLALAE VFRAPLRPDV VRFVHRLLSC NKRQPYAVSR RAGHQTSAES WGTGRAVSRI PRVPGGGTH RAGQGAFGNM CRGGRMFAPT KTWRKWHRRV NVHLRRVAVA SALAATSVPS LVLARGHRIE TVPELPLVIS D SAESIEKT ...String:
MATQTRPLVS VKALEGDMAT DNSSSLALAE VFRAPLRPDV VRFVHRLLSC NKRQPYAVSR RAGHQTSAES WGTGRAVSRI PRVPGGGTH RAGQGAFGNM CRGGRMFAPT KTWRKWHRRV NVHLRRVAVA SALAATSVPS LVLARGHRIE TVPELPLVIS D SAESIEKT SQAIKILKQV GAYADAEKAK DSVGIRPGKG KMRNRRYINR KGPLIVYGTE GSKIVKAFRN LPGVDVANVE RL NLLDLAP GGHLGRFVIW TESAFKKLEE VYGTFEAPSL KKKGFILPRP KMANADLGRI INSDEVQSVV KPLNKEVKRR EKR KNPLKN VAAVLKLNPY FGTARKMATL AEAARIKARK EKLDSKRTKL SPEEAAKVKA AGKAWYKTMI SDSDYAEFDN FSKW LGVTQ

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Macromolecule #7: Ribosomal protein L5 eukaryotic C-terminal domain-containing protein

MacromoleculeName: Ribosomal protein L5 eukaryotic C-terminal domain-containing protein
type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 35.341051 KDa
SequenceString: MFWMDFQVFV KNQKTRAYSK RFQVKFKRRR QGKTDYRARL RLTNQDKNKY NTPKYRFVVR FTNKDVTAQI VYATIAGDIV MAAAYSHEL PRYGLEVGLT NYAAAYCTGL LLARRVLKCR DLDQEYEGNV EATGEDFSVE PADERRPFRA LLDVGLIRTT T GNRVFGAL ...String:
MFWMDFQVFV KNQKTRAYSK RFQVKFKRRR QGKTDYRARL RLTNQDKNKY NTPKYRFVVR FTNKDVTAQI VYATIAGDIV MAAAYSHEL PRYGLEVGLT NYAAAYCTGL LLARRVLKCR DLDQEYEGNV EATGEDFSVE PADERRPFRA LLDVGLIRTT T GNRVFGAL KGALDGGLDI PHSDKRFAGF KKDEKQLDAE IHRKYIYGGH VADYMKSLAD EEPEKYQSHF SEYIKKGIEA DN MEALYKK VHAAIRADPT MAKSTKEPPK THKRYNPKKL TYEQRKASLV ERLNALNSSA GADVDEDDDE

UniProtKB: Large ribosomal subunit protein uL18 C-terminal eukaryotes domain-containing protein

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Macromolecule #8: 60S ribosomal protein L6

MacromoleculeName: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 25.924109 KDa
SequenceString: SPDCRSASTE ESPMAPTSKM ALGIKRASRS HTYHRRGLWA IKAKHGGALP KAEKPAAVAE PKFYPADDVK PRTVSTRKPH PTKLRSTIT PGTVLILLAG RYMGKRVVFL KQLQSGLLLI TGPFKINGVP IRRVNQAYVI ATSTKVDISK VNVQKFDDKY F AREKKTRA ...String:
SPDCRSASTE ESPMAPTSKM ALGIKRASRS HTYHRRGLWA IKAKHGGALP KAEKPAAVAE PKFYPADDVK PRTVSTRKPH PTKLRSTIT PGTVLILLAG RYMGKRVVFL KQLQSGLLLI TGPFKINGVP IRRVNQAYVI ATSTKVDISK VNVQKFDDKY F AREKKTRA KKTEGELFES DKEATKNLPD FKKDDQKVID AELIKAIDAV PDLKNYLGAR FSLRDGDKPH EMTF

UniProtKB: 60S ribosomal protein L6

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Macromolecule #9: 60S ribosomal protein uL30

MacromoleculeName: 60S ribosomal protein uL30 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 28.161137 KDa
SequenceString: MASEAAKVAV PEKVILKRRR EEVWAAEKKQ KVAAEKIKAA ENTKVIYARA EQYAGEYEAQ DKELVQLKRE ARMKGGFYVS PEAKLLFVV RIRGINAMHP KTKKILQLLR LRQIFNGVFL KVNKATINML RRVEPYVAYG YPNLKSVREL IYKRGYGKLN K QRIPLANN ...String:
MASEAAKVAV PEKVILKRRR EEVWAAEKKQ KVAAEKIKAA ENTKVIYARA EQYAGEYEAQ DKELVQLKRE ARMKGGFYVS PEAKLLFVV RIRGINAMHP KTKKILQLLR LRQIFNGVFL KVNKATINML RRVEPYVAYG YPNLKSVREL IYKRGYGKLN K QRIPLANN KVIEEGLGKH NIICIEDLVH EILTVGPHFK EANNFLWPFK LKAPLGGLKK KRNHYVEGGD AGNRENYINQ LV RRMN

UniProtKB: Uncharacterized protein

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Macromolecule #10: 60S ribosomal protein L7a

MacromoleculeName: 60S ribosomal protein L7a / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 29.407152 KDa
SequenceString: MAPKRGGKAP VPAKKKTDKV TNPLFEKRPK QFGIGGALPP KKDLHRFVKW PKVVRIQRQR RILKQRLKVP PALHQFTRTL DKNLATNLF KMLLKYRPED KAAKKERLLK RAQAEAEGKT VEAKKPIVVK YGLNHVTYLI EQSKAQLVVI AHDVDPIELV V WLPALCRK ...String:
MAPKRGGKAP VPAKKKTDKV TNPLFEKRPK QFGIGGALPP KKDLHRFVKW PKVVRIQRQR RILKQRLKVP PALHQFTRTL DKNLATNLF KMLLKYRPED KAAKKERLLK RAQAEAEGKT VEAKKPIVVK YGLNHVTYLI EQSKAQLVVI AHDVDPIELV V WLPALCRK MEVPYCIVKG KSRLGSIVHK KTASVLCLTT VKNEDKLEFS KILEAIKANF NDKFDEVRKK WGGGVMGSKS QA KTKARER LIAKEAAQRM N

UniProtKB: 60S ribosomal protein L7a

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Macromolecule #11: Ribosomal protein L6 alpha-beta domain-containing protein

MacromoleculeName: Ribosomal protein L6 alpha-beta domain-containing protein
type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 21.301918 KDa
SequenceString:
MKTILASETM EIPEEVTVKV SAKMISVTGP RGTLTRNFKH LNLDFQLQEG GRKLKVDAWF GTRKTMAAIR TAISHVQNLI TGVTKGFRY KMRFVYAHFP INASITAANK GIEIRNFLGE KKVRKVDMLD GVTILRSEKV KDEIVLDGND IELVSRSAAL I NQKCHVKN KDIRKFLDGI YVSDKGAIKE E

UniProtKB: Large ribosomal subunit protein uL6 alpha-beta domain-containing protein

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Macromolecule #12: Ribosomal protein L10e/L16 domain-containing protein

MacromoleculeName: Ribosomal protein L10e/L16 domain-containing protein / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 24.516867 KDa
SequenceString: MGRRPARCYR QIKNKPYPKS RYCRGVPDPK IRIYDVGMKK KGVDEFPSCV HLVSWEKENV SSEALEAARI ACNKYMTKNA GKDAFHLRV RVHPYHVLRI NKMLSCAGAD RLQTGMRGAF GKPLGTCARV DIGQVLLSVR CKESNAVHAD EALRRAKFKF P GRQKIIRS ...String:
MGRRPARCYR QIKNKPYPKS RYCRGVPDPK IRIYDVGMKK KGVDEFPSCV HLVSWEKENV SSEALEAARI ACNKYMTKNA GKDAFHLRV RVHPYHVLRI NKMLSCAGAD RLQTGMRGAF GKPLGTCARV DIGQVLLSVR CKESNAVHAD EALRRAKFKF P GRQKIIRS RKWGFTKFNR ADYIKYKSEG RIVPDGVNAK LLGVHGPIAK RAPGQAILA

UniProtKB: Uncharacterized protein

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Macromolecule #13: 60S ribosomal protein L11

MacromoleculeName: 60S ribosomal protein L11 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 20.725115 KDa
SequenceString:
MSTEKKQLNP MREIKVQKLV LNISVGESGD RLTRAAKVLE QLSGQSPVFS KARYTVRSFG IRRNEKIACY VTIRGEKAMQ LLESGLKVK EYELLRRNFS DTGCFGFGIQ EHIDLGMKYD PSTGIYGMDF FVVLERAGYR VSRRRRCKAR VGIHQRVTKE D AMKWFQVK YEGVILNKSH AS

UniProtKB: 60S ribosomal protein L11

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Macromolecule #14: 60S ribosomal protein L13

MacromoleculeName: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 24.170293 KDa
SequenceString: MVKHNNVIPN GHFKKHWQNY VKTWFNQPAR KQRRRIARQK NAVKIFPRPT SGPLRPIVQC QTRKYNMKAR AGRGFTLEEL KSAGIPKKL APTIGISVDH RRKNRSLEGM QSNIQRLKTY KAKLVIFPRR ARKVKAGDST PEELANATQV QGDYMPIARG E KRSVEVVK ...String:
MVKHNNVIPN GHFKKHWQNY VKTWFNQPAR KQRRRIARQK NAVKIFPRPT SGPLRPIVQC QTRKYNMKAR AGRGFTLEEL KSAGIPKKL APTIGISVDH RRKNRSLEGM QSNIQRLKTY KAKLVIFPRR ARKVKAGDST PEELANATQV QGDYMPIARG E KRSVEVVK VTEEMKEFKA YGKLRVERMN QRQLGARQKK AAEAEKEEKK

UniProtKB: 60S ribosomal protein L13

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Macromolecule #15: Ribosomal protein L14e domain-containing protein

MacromoleculeName: Ribosomal protein L14e domain-containing protein / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 15.490531 KDa
SequenceString:
MPFKRFVEIG RVALVNYGKD YGRLVVIVDV VDQNRALVDA PDMVRCQMNF KRLSLTDIKI DIKRIPKKAT LIKAMEEADV KTKWENSSW GKKLIVQKKR AALNDFDRFK VMLAKIKRGG AIRQELAKLK KEVAAA

UniProtKB: Large ribosomal subunit protein eL14 domain-containing protein

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Macromolecule #16: Ribosomal protein L15

MacromoleculeName: Ribosomal protein L15 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 24.488666 KDa
SequenceString: MGAYKFVSEL WRRKQSDVMR FVQRVRCWEY RQQPAIVRIT RPTRPDRARR LGFKAKQGYV VYRIRVRRGG RKRPVPKGIV YGKPKHQGI TQLKFQRNKR SVAEERAGRR LGGLRVLNSY WVNEDSTYKY FEVILVDVAH SAVRNDPRIN WLCKPVHKHR E LRGLTSAG ...String:
MGAYKFVSEL WRRKQSDVMR FVQRVRCWEY RQQPAIVRIT RPTRPDRARR LGFKAKQGYV VYRIRVRRGG RKRPVPKGIV YGKPKHQGI TQLKFQRNKR SVAEERAGRR LGGLRVLNSY WVNEDSTYKY FEVILVDVAH SAVRNDPRIN WLCKPVHKHR E LRGLTSAG KKFRGLRGKG HRHHKNRPSR RATWKRNQTV SLRRYR

UniProtKB: Ribosomal protein L15

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Macromolecule #17: Ribosomal protein L13a

MacromoleculeName: Ribosomal protein L13a / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 23.581172 KDa
SequenceString: MVSGSGVCAK RVVVDARHHM LGRLASIVAK ELLNGQRVVV VRCEEICMSG GLVRQKMKYL RFLRKRMNTK PSHGPIHFRA PAKILWRTI RGMIPHKTAR GEAALARLKA YEGVPPPYDR TKRMVIPDAL KVLRLQPGHR YCLLGQLSKE VGWNYADTIR E LEEKRKEK ...String:
MVSGSGVCAK RVVVDARHHM LGRLASIVAK ELLNGQRVVV VRCEEICMSG GLVRQKMKYL RFLRKRMNTK PSHGPIHFRA PAKILWRTI RGMIPHKTAR GEAALARLKA YEGVPPPYDR TKRMVIPDAL KVLRLQPGHR YCLLGQLSKE VGWNYADTIR E LEEKRKEK AKVSYDRRKQ LAKLRVKAEK AAEEKLGTQL EILAPIKY

UniProtKB: Ribosomal protein L13a

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Macromolecule #18: 60S ribosomal protein uL22

MacromoleculeName: 60S ribosomal protein uL22 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 19.525549 KDa
SequenceString:
MVKYSTDPAN PTKSAKAMGR DLRVHFKNTR ETAFALRKMS LNKAKRYLED VLAHKQAIPF RRYCRGVGRT AQVKNRQPNG QGRWPAKSA KFVLDLLKNA ESNAEVKGLD VDALYISHIQ VNQAQKQRRR TYRAHGRINP YMSNPCHIEL ILSEKEEPVK K EAESQIAR KA

UniProtKB: Uncharacterized protein

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Macromolecule #19: Ribosomal protein L18e/L15P domain-containing protein

MacromoleculeName: Ribosomal protein L18e/L15P domain-containing protein / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 21.351285 KDa
SequenceString:
MGIDLVAGGR NKRTKRVAPK SDDVYLKLLV KLYRFLVRRT KSKFNAVILK RLFMSKTNRP PLSMRRLSNF MKGKEEKNIA VVVGTITDD MRIQEVPAMK VTALRFTETA RARIINAGGE CLTFDQLALR APTGENTILL RGPKNAREAV RHFGKAPGVP H SHTKPYVR SKGRKFEKAR GRRNSRGFKV

UniProtKB: Large ribosomal subunit protein uL15/eL18 domain-containing protein

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Macromolecule #20: Ribosomal protein L19

MacromoleculeName: Ribosomal protein L19 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 24.059664 KDa
SequenceString: MVSLKLQKRL ASSVLKCGKG KVWLDPNEVN EISMANSRQN IRKLVKDGFI IRKPQKIHSR SRARRAHEAK QKGRHSGYGK RRGTREARL PTKILWMRRM RVLRRLLRKY REAKKIDKHM YHDMYLKVKG NMFKNKRVLM ESIHKSKAEK AREKTLSDQF E AKRAKSKA ...String:
MVSLKLQKRL ASSVLKCGKG KVWLDPNEVN EISMANSRQN IRKLVKDGFI IRKPQKIHSR SRARRAHEAK QKGRHSGYGK RRGTREARL PTKILWMRRM RVLRRLLRKY REAKKIDKHM YHDMYLKVKG NMFKNKRVLM ESIHKSKAEK AREKTLSDQF E AKRAKSKA SRERKIARRE ERLAQGPRDH APAPAAAAPA AAPKKAKK

UniProtKB: Ribosomal protein L19

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Macromolecule #21: 60S ribosomal protein L18a

MacromoleculeName: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 21.296041 KDa
SequenceString:
MVAHRFHQYQ VVGRALPAPG DEQPKIYRMK LWATNEVRAK SKFWYFLRKL KKVKKANGQM LAINEIFEKN PTTIKNYGIW LRYQSRTGY HNMYKEYRDT TLNGGVEQMY TEMASRHRVR APCIQIIKTA TVDFKLCKRD NTKQFHNSKI KFPLVYQKVR P PTRKLKTT YKATRPNLFM

UniProtKB: 60S ribosomal protein L18a

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Macromolecule #22: 60S ribosomal protein L21

MacromoleculeName: 60S ribosomal protein L21 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 18.719801 KDa
SequenceString:
MPAGHGLRSR TRDLFARPFR KKGYIPLTTY LRTYKVGEHV DVKVNGAVHK GMPHKFYHGR TGRVWNVTKR AIGVEINKQV GNRIIKKRI HVRVEHVQPS RCNEEFLQRK LNNDKLKAEA KVRGEVISTK RQPAGPKPGF MVEGTTIETV TPIPYDVVND L KGGY

UniProtKB: 60S ribosomal protein L21

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Macromolecule #23: Genome assembly, chromosome: II

MacromoleculeName: Genome assembly, chromosome: II / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 14.372516 KDa
SequenceString:
MARGAAAAAA AAKGKKKGSV SFVIDCTKPV EDKIMEIASL EKFLQERIKV AGGKAGNLGD SVTVTRDKSK VTVTSDGAFS KRYLKYLTK KYLKKHNVRD WLRVIAANKE RNVYELRYFN IAENEGEEED

UniProtKB: Uncharacterized protein

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Macromolecule #24: Ribosomal protein L17

MacromoleculeName: Ribosomal protein L17 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 14.536206 KDa
SequenceString:
GRGGSAGNKF RMSLGLPVAA TVNCADNTGA KNLYIISVKG IKGRLNRLPS ACVGDMVMAT VKKGKPDLRK KVMPAVIVRQ RKPWRRKDG VFMYFEDNAG VIVNPKGEMK GSAITGPIGK ECADLWPRIA SAANAIV

UniProtKB: Ribosomal protein L17

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Macromolecule #25: TRASH domain-containing protein

MacromoleculeName: TRASH domain-containing protein / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 18.293646 KDa
SequenceString:
MVLKTELCRF SGQKIYPGKG IRFIRSDSQV FLFANSKCKR YFHNRLKPAK LCWTAMYRKQ HKKDIHAEAA KKRRRTTKKP YSRSIVGAT LEVIQKKRAE KPEVRDAARE AALEIKERIK KTKDEKKAKK AEVTKSQKSQ GGKGAVQKGS KGPKIGGGGG K R

UniProtKB: TRASH domain-containing protein

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Macromolecule #26: Genome assembly, chromosome: II

MacromoleculeName: Genome assembly, chromosome: II / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 16.920203 KDa
SequenceString:
MAPKVAVAKK GDAKAQAAKV AKAVKSGSIK KTAKKIRTSV TFHRPKTLSK ARDPKYPRIS TPGRNKLDQY QILKYPLTTE SAMKKIEDN NTLVFIVDLK ADKKKIKAAV KKMYDIQAKK VNTLIRPDGK KKAYVKLTPD YDALDVANKI GII

UniProtKB: Large ribosomal subunit protein uL23 N-terminal domain-containing protein

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Macromolecule #27: KOW domain-containing protein

MacromoleculeName: KOW domain-containing protein / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 14.73538 KDa
SequenceString:
MKRNPRVTSS RRKCRKAHFT APSSVRRVLM SAALSSELRH KYNVRSIPIR KDDEVQVVRG TYKGREGKVV QVYRRRWVIH VERITREKV NGSTVNVGIH PSKVIVTKLK LDKDRKALLD RKARGRAA

UniProtKB: KOW domain-containing protein

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Macromolecule #28: 60S ribosomal protein L27

MacromoleculeName: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 15.615687 KDa
SequenceString:
MVKFLKPGKA VILLQGRFAG RKAVIVRVFE EGTRDRPYGH CLVAGLAKYP KKVIRKDSAK KTAKKSRVKC FLKLVNFTHI MPTRYTLDV DFKDVASGGP DALATRDKKV ASCKAAKARL EERFKTGKNR WFFTKLRF

UniProtKB: 60S ribosomal protein L27

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Macromolecule #29: Ribosomal protein L18e/L15P domain-containing protein

MacromoleculeName: Ribosomal protein L18e/L15P domain-containing protein / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 16.068989 KDa
SequenceString:
MTTRFKKNRK KRGHVSAGHG RVGKHRKHPG GRGNAGGMHH HRILFDKYHP GYFGKVGMRY FHKLSNRFYT PTVNVERLWS MVPSEKAAE AGGDKAPVVD VSQFGYFKVL GKGMLPEKPI VVKAKLISKI AEKKIKAAGG AVVLVA

UniProtKB: Large ribosomal subunit protein uL15/eL18 domain-containing protein

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Macromolecule #30: 60S ribosomal protein L29

MacromoleculeName: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 6.994063 KDa
SequenceString:
MAKSKNHTAH NQSYKAHRNG IKKPKRQRQA STKGMDPKFL RNLRYSRKHN VKAGEGEVEK

UniProtKB: 60S ribosomal protein L29

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Macromolecule #31: Ribosomal protein L7Ae/L30e/S12e/Gadd45 domain-containing protein

MacromoleculeName: Ribosomal protein L7Ae/L30e/S12e/Gadd45 domain-containing protein
type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 12.372487 KDa
SequenceString:
MAPTKKAKKS GENINNKLQL VMKSGKYTLG YKTVLKTLRS SKGKLIILAN NCPPLRKSEI EYYAMLAKIS VHHFHGNNVD LGTACGKYY RVCCLSILDP GDSDIISTTT TTQ

UniProtKB: Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein

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Macromolecule #32: 60S ribosomal protein L31

MacromoleculeName: 60S ribosomal protein L31 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 14.152365 KDa
SequenceString:
MSEKKRAPGP RKDEVVTREY TVNLHKRLHG CTFKKKAPNA IKEIRKFAQK AMGTNDVRID VKLNKHIWSS GIRSVPRRVR VRIARKRND EEDAKEELYS LVTVAEVPQE GLKGLGTKVV EDED

UniProtKB: 60S ribosomal protein L31

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Macromolecule #33: 60S ribosomal protein eL32

MacromoleculeName: 60S ribosomal protein eL32 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 15.737636 KDa
SequenceString:
MAVPLLTRKI VKKRVKHFKR AHSDRYIGLK QSWRRPKGID SRVRRKFKGC TLMPNIGYGS DKKTRHYLPN KFKKFVVHNV SELELLMMH NRTYCAEIAH NVSTKKRKDI VERAAQLDIV VTNKLARLRS QEDE

UniProtKB: 60S ribosomal protein L32

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Macromolecule #34: 60S ribosomal protein eL33

MacromoleculeName: 60S ribosomal protein eL33 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 12.68473 KDa
SequenceString:
MKGRQGQRVR LYVRGTILGY KRSKSNQYEN TSLLQIEGVN TKEEVGWYAG KRIAYVYKAK TKSNDSTIRC IWGKVTRPHG NSGVVRAKF RSNLPPTSMG KKVRVFMYPS SI

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Macromolecule #35: 60S ribosomal protein L34

MacromoleculeName: 60S ribosomal protein L34 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 13.798365 KDa
SequenceString:
MVQRLTYRKR HSYATKSNQT RVVKTPGGKL VYQYTKKRAS GPKCPVTGKK IQGIPHLRPT EYKRSRLSRN RRTVNRPYGG VLSGPAVRE RIIRAFLVEE QKIVKKVLKI QKTKEKTTKS

UniProtKB: 60S ribosomal protein L34

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Macromolecule #36: 60S ribosomal protein L35

MacromoleculeName: 60S ribosomal protein L35 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 14.401041 KDa
SequenceString:
MSSGKVKAGE LWNKSKDDLT KQLAELKTEL GQLRIQKVAS SGSKLNRIHD IRKSIARVLT VINAKQRAQL RLFYKNKKYA PLDLRAKQT RAIRRRLSPD EKSRVLEKTK KRTVHFPQRK FAIKA

UniProtKB: Large ribosomal subunit protein uL29

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Macromolecule #37: 60S ribosomal protein L36

MacromoleculeName: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 12.594097 KDa
SequenceString:
MAPSQPKSGL FVGINKGHVV TKRELPPRPS DRKGKGTKRV HFVRNLIREV AGFAPYEKRI TELLKVGKDK RALKVAKRKL GTHKRAKKK REEMSSVLRK MRSAGTGTEK KK

UniProtKB: 60S ribosomal protein L36

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Macromolecule #38: Ribosomal protein L37

MacromoleculeName: Ribosomal protein L37 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 10.765555 KDa
SequenceString:
MTKGTGSFGK RRNKTHTLCI RCGRRSFHLQ KSTCSSCGYP AARIRKYNWS VKAIRRKTTG TGRMRYMRHV PRRFKSNFRE GTEAAPRNR AAAN

UniProtKB: Ribosomal protein L37

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Macromolecule #39: 60S ribosomal protein L38

MacromoleculeName: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 8.115617 KDa
SequenceString:
MPKQIHEIKD FLLTARRKDA RSVRIKRTKD AVKFKVRCSR YLYTLCVHDT DKANKLKQSL PPGLNVQEV

UniProtKB: 60S ribosomal protein L38

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Macromolecule #40: Ribosomal protein L39

MacromoleculeName: Ribosomal protein L39 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 6.528786 KDa
SequenceString:
MPSHKTFRIK QKLAKKQRQN RPIPYWIRMR TDNTIRYNAK RRHWRRTKLG F

UniProtKB: Ribosomal protein L39

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Macromolecule #41: Ubiquitin-like domain-containing protein

MacromoleculeName: Ubiquitin-like domain-containing protein / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 14.82842 KDa
SequenceString:
MQIFVKTLTG KTITLEVESS DTIDNVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLADYN IQKESTLHLV LRLRGGIIEP SLQALARKY NQEKMICRKC YARLHPRAVN CRKKKCGHSN QLRPKKKIKN

UniProtKB: Ubiquitin-like domain-containing protein

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Macromolecule #42: Genome assembly, chromosome: II

MacromoleculeName: Genome assembly, chromosome: II / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 12.152455 KDa
SequenceString:
MVNVPKTKKT YCKNKECKKH TLHKVTQYKK GKDSLSAQGK RRYDRKQSGY GGQT(MLZ)PVFHK KAKTTKKIVL KLQCQS CKH YSQRAIKRCK HFEIGGDKKG KGTSLF

UniProtKB: Uncharacterized protein

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Macromolecule #43: 60S ribosomal protein L37a, expressed

MacromoleculeName: 60S ribosomal protein L37a, expressed / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 10.266146 KDa
SequenceString:
MTKRTKKAGI VGKYGTRYGA SLRKQIKKME VSQHSKYFCE FCGKFAVKRK AVGIWGCKDC GKVKAGGAYT MNTASAVTVR STIRRLREQ TEA

UniProtKB: 60S ribosomal protein L37a, expressed

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Macromolecule #44: Ribosomal L28e/Mak16 domain-containing protein

MacromoleculeName: Ribosomal L28e/Mak16 domain-containing protein / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Triticum aestivum (bread wheat)
Molecular weightTheoretical: 16.607375 KDa
SequenceString:
MTTVPGSLVW ELVKKNNCFL IKQFGNSNAK VQFSKEPNNL YNVHSYKFSG LANSKTVAVQ PSAGEDKAVV LSTTKTKKQN TPAKLQHKT LMRKEFRKMA KSVKNQVCDN YYRPDLTKPA LARLSAVYRS LQVSKSGIKK KNRQPTKL

UniProtKB: Large ribosomal subunit protein eL28

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Macromolecule #45: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 45 / Number of copies: 72 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #46: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 46 / Number of copies: 242 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #47: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 47 / Number of copies: 3 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.6
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 289 K / Instrument: FEI VITROBOT MARK IV
DetailsThe sample is the large subunit of wheat ribosome

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Average electron dose: 44.6 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 2.7 µm / Calibrated magnification: 75000 / Illumination mode: OTHER / Imaging mode: OTHER / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.5 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

4v7e

Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.84 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 105563
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: NOT APPLICABLE
FSC plot (resolution estimation)

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