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- EMDB-35823: Cryo-EM structure of the 48-nm repeat doublet microtubule from mo... -
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Open data
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Basic information
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Title | Cryo-EM structure of the 48-nm repeat doublet microtubule from mouse sperm | |||||||||
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![]() | doublet microtubule / ![]() | |||||||||
Function / homology | ![]() male germ-line stem cell population maintenance / sperm flagellum assembly / outer dense fiber / manchette assembly / outer acrosomal membrane / axonemal B tubule inner sheath / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Zhou LN / Gui M / Wu JP | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Structures of sperm flagellar doublet microtubules expand the genetic spectrum of male infertility. Authors: Lunni Zhou / Haobin Liu / Siyu Liu / Xiaoyu Yang / Yue Dong / Yun Pan / Zhuang Xiao / Beihong Zheng / Yan Sun / Pengyu Huang / Xixi Zhang / Jin Hu / Rui Sun / Shan Feng / Yi Zhu / Mingxi Liu ...Authors: Lunni Zhou / Haobin Liu / Siyu Liu / Xiaoyu Yang / Yue Dong / Yun Pan / Zhuang Xiao / Beihong Zheng / Yan Sun / Pengyu Huang / Xixi Zhang / Jin Hu / Rui Sun / Shan Feng / Yi Zhu / Mingxi Liu / Miao Gui / Jianping Wu / ![]() Abstract: Sperm motility is crucial for successful fertilization. Highly decorated doublet microtubules (DMTs) form the sperm tail skeleton, which propels the movement of spermatozoa. Using cryo-electron ...Sperm motility is crucial for successful fertilization. Highly decorated doublet microtubules (DMTs) form the sperm tail skeleton, which propels the movement of spermatozoa. Using cryo-electron microscopy (cryo-EM) and artificial intelligence (AI)-based modeling, we determined the structures of mouse and human sperm DMTs and built an atomic model of the 48-nm repeat of the mouse sperm DMT. Our analysis revealed 47 DMT-associated proteins, including 45 microtubule inner proteins (MIPs). We identified 10 sperm-specific MIPs, including seven classes of Tektin5 in the lumen of the A tubule and FAM166 family members that bind the intra-tubulin interfaces. Interestingly, the human sperm DMT lacks some MIPs compared with the mouse sperm DMT. We also discovered variants in 10 distinct MIPs associated with a subtype of asthenozoospermia characterized by impaired sperm motility without evident morphological abnormalities. Our study highlights the conservation and tissue/species specificity of DMTs and expands the genetic spectrum of male infertility. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 325.1 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 76 KB 76 KB | Display Display | ![]() |
Images | ![]() | 153.3 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8iyjMC C: citing same article ( M: atomic model generated by this map |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 1.087 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
+Entire : Mouse sperm DMT
+Supramolecule #1: Mouse sperm DMT
+Macromolecule #1: Cilia- and flagella-associated protein 95
+Macromolecule #2: EF-hand domain-containing family member B
+Macromolecule #3: Cilia- and flagella-associated protein 53
+Macromolecule #4: Nucleoside diphosphate kinase 7
+Macromolecule #5: Cilia- and flagella-associated protein 107
+Macromolecule #6: Cilia- and flagella-associated protein 141
+Macromolecule #7: Tektin-1
+Macromolecule #8: Tubulin alpha-3 chain
+Macromolecule #9: Tubulin beta-4B chain
+Macromolecule #10: Meiosis-specific nuclear structural protein 1
+Macromolecule #11: Tektin-2
+Macromolecule #12: Tektin-3
+Macromolecule #13: Sperm-associated antigen 8
+Macromolecule #14: Tektin-4
+Macromolecule #15: Cilia- and flagella-associated protein 161
+Macromolecule #16: Piercer of microtubule wall 2 protein
+Macromolecule #17: Protein FAM166A
+Macromolecule #18: Cilia- and flagella-associated protein 68
+Macromolecule #19: Cilia and flagella-associated protein 77
+Macromolecule #20: Family with sequence similarity 183, member B
+Macromolecule #21: Cilia- and flagella-associated protein 90
+Macromolecule #22: Protein FAM166C
+Macromolecule #23: RIB43A-like with coiled-coils protein 1
+Macromolecule #24: Dual specificity phosphatase 21
+Macromolecule #25: EF-hand domain-containing protein 1
+Macromolecule #26: Testis expressed 49
+Macromolecule #27: Testis-expressed sequence 37 protein
+Macromolecule #28: Tektin bundle-interacting protein 1
+Macromolecule #29: RIB43A-like with coiled-coils protein 2
+Macromolecule #30: Tektin-5
+Macromolecule #31: EF-hand domain-containing family member C2
+Macromolecule #32: Spermatid-specific manchette-related protein 1
+Macromolecule #33: Protein phosphatase 1 regulatory subunit 32
+Macromolecule #34: Testis-specific serine/threonine-protein kinase 6
+Macromolecule #35: Cilia- and flagella-associated protein 20
+Macromolecule #36: Outer dense fiber protein 3
+Macromolecule #37: Parkin coregulated gene protein homolog
+Macromolecule #38: Testis, prostate and placenta-expressed protein
+Macromolecule #39: Cilia- and flagella-associated protein 45
+Macromolecule #40: Uncharacterized protein C10orf82 homolog
+Macromolecule #41: Coiled-coil domain-containing protein 105
+Macromolecule #42: Cilia- and flagella-associated protein 52
+Macromolecule #43: Enkurin
+Macromolecule #44: Testis-expressed protein 43
+Macromolecule #45: Protein Flattop
+Macromolecule #46: EF-hand calcium-binding domain-containing protein 6
+Macromolecule #47: Cilia- and flagella-associated protein 210
+Macromolecule #48: Cilia- and flagella-associated protein 276
+Macromolecule #49: Piercer of microtubule wall 1 protein
+Macromolecule #50: GUANOSINE-5'-TRIPHOSPHATE
+Macromolecule #51: MAGNESIUM ION
+Macromolecule #52: GUANOSINE-5'-DIPHOSPHATE
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | filament |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Number real images: 25135 / Average exposure time: 2.56 sec. / Average electron dose: 50.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: EMDB MAP EMDB ID: |
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Initial angle assignment | Type: NOT APPLICABLE |
Final angle assignment | Type: NOT APPLICABLE |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 95290 |