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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | Tom core complex with Tom20 and Tom22 subunits. | |||||||||
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![]() | Tom complex / Tom20 / Cryo-EM / TRANSLOCASE | |||||||||
Function / homology | ![]() TOM complex / mitochondrion targeting sequence binding / mitochondrial outer membrane translocase complex / positive regulation of mitophagy in response to mitochondrial depolarization / protein import into mitochondrial matrix / Mitochondrial protein import / protein targeting to mitochondrion / positive regulation of protein targeting to mitochondrion / protein insertion into mitochondrial outer membrane / protein transmembrane transporter activity ...TOM complex / mitochondrion targeting sequence binding / mitochondrial outer membrane translocase complex / positive regulation of mitophagy in response to mitochondrial depolarization / protein import into mitochondrial matrix / Mitochondrial protein import / protein targeting to mitochondrion / positive regulation of protein targeting to mitochondrion / protein insertion into mitochondrial outer membrane / protein transmembrane transporter activity / PINK1-PRKN Mediated Mitophagy / regulation of protein stability / mitochondrial outer membrane / mitochondrion / membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.54 Å | |||||||||
![]() | Liu DS / Sui SF | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Tom core complex with Tom20 and Tom22 subunits. Authors: Liu DS / Sui SF | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 62.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 13.8 KB 13.8 KB | Display Display | ![]() |
Images | ![]() | 73.5 KB | ||
Filedesc metadata | ![]() | 5.5 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7vbyMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 0.8433 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
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Sample components
-Entire : Tox complex
Entire | Name: Tox complex |
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Components |
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-Supramolecule #1: Tox complex
Supramolecule | Name: Tox complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#5 |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: Translocase of the Outer Membrane
Macromolecule | Name: Translocase of the Outer Membrane / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 89.999117 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MGNVLAASSP PAGPPPPPAP ALVGLPPPPP SPPGFTLPPL GGSLGAGTST SRSSERTPGA ATASASGAAE DGACGCLPNP GTFEECHRK CKELFPIQME GVKLTVNKGL SNHFQVNHTV ALSTIGESNY HFGVTYVGTK QLSPTEAFPV LVGDMDNSGS L NAQVIHQL ...String: MGNVLAASSP PAGPPPPPAP ALVGLPPPPP SPPGFTLPPL GGSLGAGTST SRSSERTPGA ATASASGAAE DGACGCLPNP GTFEECHRK CKELFPIQME GVKLTVNKGL SNHFQVNHTV ALSTIGESNY HFGVTYVGTK QLSPTEAFPV LVGDMDNSGS L NAQVIHQL GPGLRSKMAI QTQQSKFVNW QVDGEYRGSD FTAAVTLGNP DVLVGSGILV AHYLQSITPC LALGGELVYH RR PGEEGTV MSLAGKYTLN NWLATVTLGQ AGMHATYYHK ASDQLQVGVE FEASTRMQDT SVSFGYQLDL PKANLLFKGS VDS NWIVGA TLEKKLPPLP LTLALGAFLN HRKNKFQCGF GLTIGMVGRN SAIAAGVCGA LFIGYCIYFD RKRRSDPNFK NRLR ERRKK QKLAKERAGL SKLPDLKDAE AVQKFFLEEI QLGEELLAQG EYEKGVDHLT NAIAVCGQPQ QLLQVLQQTL PPPVF QMLL TKLPTISQRI VSAQSLAEDD VEMAAAVAAA GAGEPQSPDE LLPKGDAEKP EEELEEDDDE ELDETLSERL WGLTEM FPE RVRSAAGATF DLSLFVAQKM YRFSRAALWI GTTSFMILVL PVVFETEKLQ MEQQQQLQQR QILLGPNTGL SGGMPGA LP SLPGKIMFRI EGLAPKLDPE EMKRKMREDV ISSIRNFLIY VALLRVTPFI LKKLDSIMAS STVPVSAAGS ANETPEIP D NVGDWLRGVY RFATDRNDFR RNLILNLGLF AAGVWLARNL SDIDLMAPQP GVMVKLSKEA KQRLQQLFKG SQFAIRWGF IPLVIYLGFK RGADPGMPEP TVLSLLWG |
-Macromolecule #2: Mitochondrial import receptor subunit TOM6 homolog
Macromolecule | Name: Mitochondrial import receptor subunit TOM6 homolog / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 8.007988 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MASSTVPVSA AGSANETPEI PDNVGDWLRG VYRFATDRND FRRNLILNLG LFAAGVWLAR NLSDIDLMAP QPGV UniProtKB: Mitochondrial import receptor subunit TOM6 homolog |
-Macromolecule #3: Mitochondrial import receptor subunit TOM5 homolog
Macromolecule | Name: Mitochondrial import receptor subunit TOM5 homolog / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 6.045318 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MFRIEGLAPK LDPEEMKRKM REDVISSIRN FLIYVALLRV TPFILKKLDS I UniProtKB: Mitochondrial import receptor subunit TOM5 homolog |
-Macromolecule #4: Mitochondrial import receptor subunit TOM7 homolog
Macromolecule | Name: Mitochondrial import receptor subunit TOM7 homolog / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 6.256473 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MVKLSKEAKQ RLQQLFKGSQ FAIRWGFIPL VIYLGFKRGA DPGMPEPTVL SLLWG UniProtKB: Mitochondrial import receptor subunit TOM7 homolog |
-Macromolecule #5: Mitochondrial import receptor subunit TOM22 homolog
Macromolecule | Name: Mitochondrial import receptor subunit TOM22 homolog / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 15.532528 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: MAAAVAAAGA GEPQSPDELL PKGDAEKPEE ELEEDDDEEL DETLSERLWG LTEMFPERVR SAAGATFDLS LFVAQKMYRF SRAALWIGT TSFMILVLPV VFETEKLQME QQQQLQQRQI LLGPNTGLSG GMPGALPSLP GKI UniProtKB: Mitochondrial import receptor subunit TOM22 homolog |
-Macromolecule #6: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
Macromolecule | Name: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / type: ligand / ID: 6 / Number of copies: 11 / Formula: PC1 |
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Molecular weight | Theoretical: 790.145 Da |
Chemical component information | ![]() ChemComp-PC1: |
-Macromolecule #7: UNDECANE
Macromolecule | Name: UNDECANE / type: ligand / ID: 7 / Number of copies: 12 / Formula: UND |
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Molecular weight | Theoretical: 156.308 Da |
Chemical component information | ![]() ChemComp-UND: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | cell |
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Sample preparation
Buffer | pH: 7.4 |
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Sugar embedding | Material: DIG |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: PDB ENTRY PDB model - PDB ID: |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 2.54 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 288736 |
Initial angle assignment | Type: COMMON LINE |
Final angle assignment | Type: COMMON LINE |