+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-31529 | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Title | Fivefold block reconstruction of the Melbournevirus capsid | |||||||||||||||
Map data | Block reconstruction of five-fold vertex of Melbournevirus | |||||||||||||||
Sample |
| |||||||||||||||
Function / homology | Major capsid protein, N-terminal / Major capsid protein N-terminus / Major capsid protein, C-terminal / Major capsid protein, C-terminal domain superfamily / Large eukaryotic DNA virus major capsid protein / Group II dsDNA virus coat/capsid protein / viral capsid / structural molecule activity / Major capsid protein Function and homology information | |||||||||||||||
Biological species | Melbournevirus | |||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.42 Å | |||||||||||||||
Authors | Burton-Smith RN / Murata K | |||||||||||||||
Funding support | Japan, Sweden, 4 items
| |||||||||||||||
Citation | Journal: To Be Published Title: The 4.4A structure of the giant Melbournevirus virion belonging to the Marseilleviridae family Authors: Burton-Smith RN / Reddy HKN / Svenda M / Abergel C / Okamoto K / Murata K | |||||||||||||||
History |
|
-Structure visualization
Supplemental images |
---|
-Downloads & links
-EMDB archive
Map data | emd_31529.map.gz | 54 MB | EMDB map data format | |
---|---|---|---|---|
Header (meta data) | emd-31529-v30.xml emd-31529.xml | 7.8 KB 7.8 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_31529_fsc.xml | 15.5 KB | Display | FSC data file |
Images | emd_31529.png | 162.2 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-31529 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-31529 | HTTPS FTP |
-Validation report
Summary document | emd_31529_validation.pdf.gz | 494 KB | Display | EMDB validaton report |
---|---|---|---|---|
Full document | emd_31529_full_validation.pdf.gz | 493.6 KB | Display | |
Data in XML | emd_31529_validation.xml.gz | 14.4 KB | Display | |
Data in CIF | emd_31529_validation.cif.gz | 20 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-31529 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-31529 | HTTPS FTP |
-Related structure data
Related structure data | 7yjlMC M: atomic model generated by this map C: citing same article (ref.) |
---|---|
Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
---|---|
Related items in Molecule of the Month |
-Map
File | Download / File: emd_31529.map.gz / Format: CCP4 / Size: 325 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Annotation | Block reconstruction of five-fold vertex of Melbournevirus | ||||||||||||||||||||
Voxel size | X=Y=Z: 2.21 Å | ||||||||||||||||||||
Density |
| ||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
|
-Supplemental data
-Sample components
-Entire : Melbournevirus
Entire | Name: Melbournevirus |
---|---|
Components |
|
-Supramolecule #1: Melbournevirus
Supramolecule | Name: Melbournevirus / type: virus / ID: 1 / Parent: 0 / NCBI-ID: 1560514 / Sci species name: Melbournevirus / Virus type: VIRION / Virus isolate: OTHER / Virus enveloped: No / Virus empty: No |
---|
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
---|---|
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 26.4 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |