- EMDB-28269: Mouse apoferritin heavy chain without zinc determined using singl... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-28269
タイトル
Mouse apoferritin heavy chain without zinc determined using single-particle cryo-EM with Apollo camera.
マップデータ
試料
複合体: Mouse apoferritin heavy chain
タンパク質・ペプチド: Ferritin heavy chain, N-terminally processed
リガンド: FE (III) ION
リガンド: water
キーワード
bind with iron / METAL BINDING PROTEIN
機能・相同性
機能・相同性情報
Iron uptake and transport / Golgi Associated Vesicle Biogenesis / iron ion sequestering activity / negative regulation of ferroptosis / autolysosome / ferroxidase / intracellular sequestering of iron ion / ferroxidase activity / negative regulation of fibroblast proliferation / endocytic vesicle lumen ...Iron uptake and transport / Golgi Associated Vesicle Biogenesis / iron ion sequestering activity / negative regulation of ferroptosis / autolysosome / ferroxidase / intracellular sequestering of iron ion / ferroxidase activity / negative regulation of fibroblast proliferation / endocytic vesicle lumen / Neutrophil degranulation / ferric iron binding / ferrous iron binding / iron ion transport / iron ion binding / immune response / negative regulation of cell population proliferation / mitochondrion / extracellular region / identical protein binding / membrane / cytoplasm / cytosol 類似検索 - 分子機能
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM143805
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM139616
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
GM119032
米国
引用
ジャーナル: J Struct Biol X / 年: 2023 タイトル: Characterizing the resolution and throughput of the Apollo direct electron detector. 著者: Ruizhi Peng / Xiaofeng Fu / Joshua H Mendez / Peter S Randolph / Benjamin E Bammes / Scott M Stagg / 要旨: Advances in electron detection have been essential to the success of high-resolution cryo-EM structure determination. A new generation of direct electron detector called the Apollo, has been ...Advances in electron detection have been essential to the success of high-resolution cryo-EM structure determination. A new generation of direct electron detector called the Apollo, has been developed by Direct Electron. The Apollo uses a novel event-based MAPS detector custom designed for ultra-fast electron counting. We have evaluated this new camera, finding that it delivers high detective quantum efficiency (DQE) and low coincidence loss, enabling high-quality electron counting data acquisition at up to nearly 80 input electrons per pixel per second. We further characterized the performance of Apollo for single particle cryo-EM on real biological samples. Using mouse apoferritin, Apollo yielded better than 1.9 Å resolution reconstructions at all three tested dose rates from a half-day data collection session each. With longer collection time and improved specimen preparation, mouse apoferritin was reconstructed to 1.66 Å resolution. Applied to a more challenging small protein aldolase, we obtained a 2.24 Å resolution reconstruction. The high quality of the map indicates that the Apollo has sufficiently high DQE to reconstruct smaller proteins and complexes with high-fidelity. Our results demonstrate that the Apollo camera performs well across a broad range of dose rates and is capable of capturing high quality data that produce high-resolution reconstructions for large and small single particle samples.
タンパク質・ペプチド: Ferritin heavy chain, N-terminally processed
リガンド: FE (III) ION
リガンド: water
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超分子 #1: Mouse apoferritin heavy chain
超分子
名称: Mouse apoferritin heavy chain / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1 詳細: Ferritin is a universal intracellular protein that stores iron and releases it in a controlled fashion.
由来(天然)
生物種: Mus musculus (ハツカネズミ)
分子量
理論値: 506 KDa
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分子 #1: Ferritin heavy chain, N-terminally processed