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- EMDB-27070: apo form Cryo-EM structure of Campylobacter jejune ketol-acid red... -
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Open data
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Basic information
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Title | apo form Cryo-EM structure of Campylobacter jejune ketol-acid reductoisommerase crosslinked by Glutaraldehyde | |||||||||
![]() | Campylobacter jejune ketol-acid reductoisommerase crosslinked by Glutaraldehyde | |||||||||
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![]() | ketol-acid reductoisomerase / Isomerase / enzyme stability / dodecamer / branched-chain amino acid | |||||||||
Function / homology | ![]() ketol-acid reductoisomerase (NADP+) / ketol-acid reductoisomerase activity / valine biosynthetic process / isoleucine biosynthetic process / isomerase activity / NADP binding / magnesium ion binding / cytosol Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | single particle reconstruction / Resolution: 2.94 Å | |||||||||
![]() | Zheng S / Guddat LW | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Enhancing the Thermal and Kinetic Stability of Ketol-Acid Reductoisomerase, a Central Catalyst of a Cell-Free Enzyme Cascade for the Manufacture of Platform Chemicals Authors: Lv Y / Zheng S / Goldenzweig A / Liu F / Gao Y / Yang X / Kandale A / McGeary RP / Williams S / Kobe B / Schembri MA / Landsberg MJ / Wu B / Bruck TB / Sieber V / Boden M / Rao Z / Fleishman ...Authors: Lv Y / Zheng S / Goldenzweig A / Liu F / Gao Y / Yang X / Kandale A / McGeary RP / Williams S / Kobe B / Schembri MA / Landsberg MJ / Wu B / Bruck TB / Sieber V / Boden M / Rao Z / Fleishman SJ / Schenk G / Guddat LW | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 323.6 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 14.3 KB 14.3 KB | Display Display | ![]() |
Images | ![]() | 107.9 KB | ||
Filedesc metadata | ![]() | 5.4 KB | ||
Others | ![]() ![]() | 318.4 MB 318.4 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 973.7 KB | Display | ![]() |
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Full document | ![]() | 973.2 KB | Display | |
Data in XML | ![]() | 17.4 KB | Display | |
Data in CIF | ![]() | 20.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8cy8MC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | Campylobacter jejune ketol-acid reductoisommerase crosslinked by Glutaraldehyde | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.82 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Half Map 1
File | emd_27070_half_map_1.map | ||||||||||||
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Annotation | Half Map 1 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half Map 2
File | emd_27070_half_map_2.map | ||||||||||||
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Annotation | Half Map 2 | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Campylobacter jejuni ketol-acid reductoisomerase
Entire | Name: Campylobacter jejuni ketol-acid reductoisomerase |
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Components |
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-Supramolecule #1: Campylobacter jejuni ketol-acid reductoisomerase
Supramolecule | Name: Campylobacter jejuni ketol-acid reductoisomerase / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 450 kDa/nm |
-Macromolecule #1: Ketol-acid reductoisomerase (NADP(+))
Macromolecule | Name: Ketol-acid reductoisomerase (NADP(+)) / type: protein_or_peptide / ID: 1 / Number of copies: 12 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 35.665953 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: ITVYYDKDCD LNLIKSKKVA IIGFGSQGHA HAMNLRDNGV NVTIGLREGS VSAVKAKNAG FEVMSVSEAS KIADVIMILA PDEIQADIF NVEIKPNLSE GKAIAFAHGF NIHYGQIVVP KGVDVIMIAP KAPGHTVRNE FTLGGGTPCL IAIHQDESKN A KNLALSYA ...String: ITVYYDKDCD LNLIKSKKVA IIGFGSQGHA HAMNLRDNGV NVTIGLREGS VSAVKAKNAG FEVMSVSEAS KIADVIMILA PDEIQADIF NVEIKPNLSE GKAIAFAHGF NIHYGQIVVP KGVDVIMIAP KAPGHTVRNE FTLGGGTPCL IAIHQDESKN A KNLALSYA SAIGGGRTGI IETTFKAETE TDLFGEQAVL CGGLSALIQA GFETLVEAGY EPEMAYFECL HEMKLIVDLI YQ GGIADMR YSISNTAEYG DYITGPKIIT EETKKAMKGV LKDIQNGVFA KDFILERRAG FARMHAERKN MNDSLIEKTG RNL RAMMPW IS UniProtKB: Ketol-acid reductoisomerase (NADP(+)) |
-Macromolecule #2: PENTANEDIAL
Macromolecule | Name: PENTANEDIAL / type: ligand / ID: 2 / Number of copies: 12 / Formula: PTD |
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Molecular weight | Theoretical: 100.116 Da |
Chemical component information | ![]() ChemComp-PTD: |
-Experimental details
-Structure determination
![]() | single particle reconstruction |
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Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.8 µm / Nominal defocus min: 1.2 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: NONE |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 2.94 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 74899 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |