+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-24613 | |||||||||
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Title | CLC-ec1 at pH 4.5 100 mM NaGluconate Swap | |||||||||
Map data | CLC-ec1 pH 4.5 0 mM Cl Swap | |||||||||
Sample |
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Function / homology | Chloride channel, ClcA / Chloride channel, voltage gated / Chloride channel, core / Voltage gated chloride channel / voltage-gated chloride channel activity / antiporter activity / plasma membrane / H(+)/Cl(-) exchange transporter ClcA Function and homology information | |||||||||
Biological species | Escherichia coli (E. coli) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.78 Å | |||||||||
Authors | Fortea E / Boudker O / Accardi A | |||||||||
Funding support | United States, 1 items
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Citation | Journal: To Be Published Title: Structural basis of common gate activation in CLC transporters Authors: Fortea E / Lee S / Argyros Y / Chadda R / Ciftci D / Huysmans G / Robertson JL / Boudker O / Accardi A | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_24613.map.gz | 2.2 MB | EMDB map data format | |
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Header (meta data) | emd-24613-v30.xml emd-24613.xml | 9.7 KB 9.7 KB | Display Display | EMDB header |
Images | emd_24613.png | 47.6 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-24613 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-24613 | HTTPS FTP |
-Validation report
Summary document | emd_24613_validation.pdf.gz | 327.8 KB | Display | EMDB validaton report |
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Full document | emd_24613_full_validation.pdf.gz | 327.3 KB | Display | |
Data in XML | emd_24613_validation.xml.gz | 5.8 KB | Display | |
Data in CIF | emd_24613_validation.cif.gz | 6.6 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-24613 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-24613 | HTTPS FTP |
-Related structure data
Related structure data | 7rp6MC 7n8pC 7n9wC 7rnxC 7ro0C 7rp5C 7rq7C 7rsbC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_24613.map.gz / Format: CCP4 / Size: 28.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Annotation | CLC-ec1 pH 4.5 0 mM Cl Swap | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.048 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Sample components
-Entire : Structure of ecCLC at pH 4.5 in 100mM NaGluconate
Entire | Name: Structure of ecCLC at pH 4.5 in 100mM NaGluconate |
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Components |
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-Supramolecule #1: Structure of ecCLC at pH 4.5 in 100mM NaGluconate
Supramolecule | Name: Structure of ecCLC at pH 4.5 in 100mM NaGluconate / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: Escherichia coli (E. coli) |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Molecular weight | Experimental: 100 kDa/nm |
-Macromolecule #1: H(+)/Cl(-) exchange transporter ClcA
Macromolecule | Name: H(+)/Cl(-) exchange transporter ClcA / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: Escherichia coli (E. coli) |
Molecular weight | Theoretical: 50.390402 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: MKTDTPSLET PQAARLRRRQ LIRQLLERDK TPLAILFMAA VVGTLVGLAA VAFDKGVAWL QNQRMGALVH TADNYPLLLT VAFLCSAVL AMFGYFLVRK YAPEAGGSGI PEIEGALEDQ RPVRWWRVLP VKFFGGLGTL GGGMVLGREG PTVQIGGNIG R MVLDIFRL ...String: MKTDTPSLET PQAARLRRRQ LIRQLLERDK TPLAILFMAA VVGTLVGLAA VAFDKGVAWL QNQRMGALVH TADNYPLLLT VAFLCSAVL AMFGYFLVRK YAPEAGGSGI PEIEGALEDQ RPVRWWRVLP VKFFGGLGTL GGGMVLGREG PTVQIGGNIG R MVLDIFRL KGDEARHTLL ATGAAAGLAA AFNAPLAGIL FIIEEMRPQF RYTLISIKAV FIGVIMSTIM YRIFNHEVAL ID VGKLSDA PLNTLWLYLI LGIIFGIFGP IFNKWVLGMQ DLLHRVHGGN ITKWVLMGGA IGGLCGLLGF VAPATSGGGF NLI PIATAG NFSMGMLVFI FVARVITTLL CFSSGAPGGI FAPMLALGTV LGTAFGMVAV ELFPQYHLEA GTFAIAGMGA LLAA SIRAP LTGIILVLEM TDNYQLILPM IITGLGATLL AQFTGGKPLY SAILARTLAK QEAEQLARSK AASASENT |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 1.7 mg/mL |
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Buffer | pH: 4.5 |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 294.15 K |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 64.08 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.2 µm / Nominal defocus min: 1.2 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Final reconstruction | Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.78 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 115851 |
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Initial angle assignment | Type: OTHER |
Final angle assignment | Type: OTHER |