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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | C1 of central pair | |||||||||
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Function / homology | ![]() axonemal central pair projection / : / axonemal central apparatus / axonemal central apparatus assembly / organelle / establishment of meiotic spindle localization / cilium movement involved in cell motility / cilium movement / guanylate kinase activity / axoneme assembly ...axonemal central pair projection / : / axonemal central apparatus / axonemal central apparatus assembly / organelle / establishment of meiotic spindle localization / cilium movement involved in cell motility / cilium movement / guanylate kinase activity / axoneme assembly / calcium-dependent cysteine-type endopeptidase activity / phosphopyruvate hydratase / phosphopyruvate hydratase complex / phosphopyruvate hydratase activity / adenylate kinase / adenylate kinase activity / MLL3/4 complex / Set1C/COMPASS complex / motile cilium / spindle organization / myosin phosphatase activity / protein serine/threonine phosphatase activity / protein-serine/threonine phosphatase / axoneme / chaperone cofactor-dependent protein refolding / cilium assembly / subtelomeric heterochromatin formation / enzyme regulator activity / protein folding chaperone / heat shock protein binding / tubulin binding / glycolytic process / electron transport chain / ATP-dependent protein folding chaperone / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / cilium / structural constituent of cytoskeleton / spindle pole / microtubule cytoskeleton organization / microtubule cytoskeleton / mitotic cell cycle / chromosome, telomeric region / protein refolding / microtubule binding / microtubule / calmodulin binding / hydrolase activity / negative regulation of cell population proliferation / GTPase activity / nucleotide binding / calcium ion binding / GTP binding / magnesium ion binding / ATP hydrolysis activity / proteolysis / ATP binding / membrane / nucleus / metal ion binding / cytoplasm / cytosol Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.6 Å | |||||||||
![]() | Han L / Zhang K | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM structure of an active central apparatus. Authors: Long Han / Qinhui Rao / Renbin Yang / Yue Wang / Pengxin Chai / Yong Xiong / Kai Zhang / ![]() Abstract: Accurately regulated ciliary beating in time and space is critical for diverse cellular activities, which impact the survival and development of nearly all eukaryotic species. An essential beating ...Accurately regulated ciliary beating in time and space is critical for diverse cellular activities, which impact the survival and development of nearly all eukaryotic species. An essential beating regulator is the conserved central apparatus (CA) of motile cilia, composed of a pair of microtubules (C1 and C2) associated with hundreds of protein subunits per repeating unit. It is largely unclear how the CA plays its regulatory roles in ciliary motility. Here, we present high-resolution structures of Chlamydomonas reinhardtii CA by cryo-electron microscopy (cryo-EM) and its dynamic conformational behavior at multiple scales. The structures show how functionally related projection proteins of CA are clustered onto a spring-shaped scaffold of armadillo-repeat proteins, facilitated by elongated rachis-like proteins. The two halves of the CA are brought together by elastic chain-like bridge proteins to achieve coordinated activities. We captured an array of kinesin-like protein (KLP1) in two different stepping states, which are actively correlated with beating wave propagation of cilia. These findings establish a structural framework for understanding the role of the CA in cilia. | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
-Validation report
Summary document | ![]() | 375.4 KB | Display | ![]() |
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Full document | ![]() | 375 KB | Display | |
Data in XML | ![]() | 4 KB | Display | |
Data in CIF | ![]() | 4.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7n6gMC ![]() 7n61C M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||
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Voxel size | X=Y=Z: 1.33 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
+Additional map: C1 microtubule map with overall mask, GSFSC resolution 3.49 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.41 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.11 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.19 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 2.99 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.24 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.20 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.17 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.32 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.33 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.16 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 2.94 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.29 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.13 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.00 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.20 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.06 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.09 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.37 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.04 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.17 angstrom.
+Additional map: C1a arm map with local mask, GSFSC resolution 3.18 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 2.99 angstrom.
+Additional map: C1a arm map with local mask, GSFSC resolution 3.28 angstrom.
+Additional map: C1a arm map with local mask, GSFSC resolution 3.15 angstrom.
+Additional map: C1a arm map with overall mask, GSFSC resolution 3.65 angstrom.
+Additional map: C1a arm map with local mask, GSFSC resolution 3.54 angstrom.
+Additional map: C1a arm map with local mask, GSFSC resolution 3.44 angstrom.
+Additional map: C1a arm map with local mask, GSFSC resolution 3.11 angstrom.
+Additional map: C1b arm map with local mask, GSFSC resolution 3.16 angstrom.
+Additional map: C1b arm map with local mask, GSFSC resolution 3.29 angstrom.
+Additional map: C1b arm map with local mask, GSFSC resolution 3.81 angstrom.
+Additional map: C1b arm map with local mask, GSFSC resolution 3.24 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.32 angstrom.
+Additional map: C1b arm map with local mask, GSFSC resolution 3.39 angstrom.
+Additional map: C1d complex map with local mask, GSFSC resolution 3.11 angstrom.
+Additional map: C1d complex map with local mask, GSFSC resolution 3.38 angstrom.
+Additional map: C1d complex map with local mask, GSFSC resolution 3.13 angstrom.
+Additional map: C1d complex map with over mask, GSFSC resolution 3.70 angstrom.
+Additional map: C1 F81 complex map with overall mask, GSFSC resolution 3.54 angstrom.
+Additional map: C1b arm map with local mask, GSFSC resolution 3.31 angstrom.
+Additional map: C1d complex map with local mask, GSFSC resolution 3.40 angstrom.
+Additional map: C1b arm map with over mask, GSFSC resolution 3.95 angstrom.
+Additional map: C1 F81 complex map with local mask, GSFSC resolution 3.50 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.29 angstrom.
+Additional map: C1 F81 complex map with local mask, GSFSC resolution 3.72 angstrom.
+Additional map: C1b arm map with local mask, GSFSC resolution 3.78 angstrom.
+Additional map: C1d complex map with local mask, GSFSC resolution 3.27 angstrom.
+Additional map: C1 spring layer map with local mask, GSFSC resolution 3.64 angstrom.
+Additional map: C1 spring layer map with local mask, GSFSC resolution 3.69 angstrom.
+Additional map: C1 spring layer map with local mask, GSFSC resolution 3.57 angstrom.
+Additional map: C1 spring layer map with local mask, GSFSC resolution 3.82 angstrom.
+Additional map: C1 spring layer map with local mask, GSFSC resolution 3.72 angstrom.
+Additional map: C1 spring layer map with local mask, GSFSC resolution 3.98 angstrom.
+Additional map: C1 spring layer map with local mask, GSFSC resolution 3.55 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 2.97 angstrom.
+Additional map: C1 spring layer map with local mask, GSFSC resolution 3.67 angstrom.
+Additional map: C1 spring layer map with local mask, GSFSC resolution 3.42 angstrom.
+Additional map: C1 spring layer map with local mask, GSFSC resolution 3.76 angstrom.
+Additional map: C1 spring layer map with overall mask, GSFSC...
+Additional map: C1 spring layer map with local mask, GSFSC resolution 3.54 angstrom.
+Additional map: C1 spring layer map with local mask, GSFSC resolution 3.40 angstrom.
+Additional map: C1 spring layer map with local mask, GSFSC resolution 3.79 angstrom.
+Additional map: C1 spring layer map with local mask, GSFSC resolution 3.74 angstrom.
+Additional map: C1 spring layer map with local mask, GSFSC resolution 3.49 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.30 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 3.07 angstrom.
+Additional map: C1 microtubule map with local mask, GSFSC resolution 2.98 angstrom.
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Sample components
+Entire : C1 microtubule and its associated projection of central pair
+Supramolecule #1: C1 microtubule and its associated projection of central pair
+Macromolecule #1: PF16
+Macromolecule #2: FAP194
+Macromolecule #3: FAP69
+Macromolecule #4: HYDIN
+Macromolecule #5: FAP47
+Macromolecule #6: FAP46
+Macromolecule #7: FAP54
+Macromolecule #8: HTH_9 domain-containing protein
+Macromolecule #9: FAP297
+Macromolecule #10: FAP108
+Macromolecule #11: FAP76
+Macromolecule #12: Tubulin beta
+Macromolecule #13: Tubulin alpha
+Macromolecule #14: FAP275
+Macromolecule #15: Unknown protein
+Macromolecule #16: Unknown protein
+Macromolecule #17: Unknown protein
+Macromolecule #18: Unknown protein
+Macromolecule #19: Unknown protein
+Macromolecule #20: Unknown protein
+Macromolecule #21: Unknown protein
+Macromolecule #22: Unknown protein
+Macromolecule #23: Unknown protein
+Macromolecule #24: FAP289
+Macromolecule #25: Unknown protein
+Macromolecule #26: Unknown protein
+Macromolecule #27: Unknown protein
+Macromolecule #28: FAP216
+Macromolecule #29: FAP92
+Macromolecule #30: FAP99
+Macromolecule #31: FAP74
+Macromolecule #32: FAP360
+Macromolecule #33: FAP279
+Macromolecule #34: FAP7
+Macromolecule #35: FAP81
+Macromolecule #36: FAP15
+Macromolecule #37: PF6
+Macromolecule #38: DPY30
+Macromolecule #39: FAP305
+Macromolecule #40: FAP101
+Macromolecule #41: FAP227
+Macromolecule #42: FAP114
+Macromolecule #43: FAP119
+Macromolecule #44: Calmodulin
+Macromolecule #45: Unknown protein
+Macromolecule #46: Unknown protein
+Macromolecule #47: CPC1
+Macromolecule #48: FAP246
+Macromolecule #49: Phosphopyruvate hydratase
+Macromolecule #50: Heat shock protein 70A
+Macromolecule #51: FAP42
+Macromolecule #52: Unknown protein
+Macromolecule #53: GUANOSINE-5'-DIPHOSPHATE
+Macromolecule #54: GUANOSINE-5'-TRIPHOSPHATE
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | filament |
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Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 QUANTUM (4k x 4k) / Detector mode: SUPER-RESOLUTION / Average electron dose: 38.6 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.2 µm |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 190727 |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |