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- EMDB-24191: structure of C2 projections and MIPs -

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Basic information

Entry
Database: EMDB / ID: EMD-24191
Titlestructure of C2 projections and MIPs
Map datacryo-EM map of C2 of Central Pair
Sample
  • Complex: Structure of C2 projections and MIPs
    • Protein or peptide: x 18 types
  • Ligand: x 3 types
Function / homology
Function and homology information


clathrin vesicle coat / cilium movement / motile cilium / kinesin complex / microtubule motor activity / regulation of cytoskeleton organization / clathrin binding / microtubule-based movement / axoneme / cilium assembly ...clathrin vesicle coat / cilium movement / motile cilium / kinesin complex / microtubule motor activity / regulation of cytoskeleton organization / clathrin binding / microtubule-based movement / axoneme / cilium assembly / microtubule-based process / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / phospholipid binding / structural constituent of cytoskeleton / endocytosis / microtubule binding / microtubule / hydrolase activity / endosome / GTPase activity / calcium ion binding / GTP binding / ATP hydrolysis activity / ATP binding / metal ion binding / plasma membrane / cytoplasm
Similarity search - Function
MYCBP-associated protein / Protein FAM228 / MYCBP-associated protein family / CH-like domain in sperm protein / CH-like domain in sperm protein / Kinesin-like protein / EF-hand domain pair / Kinesin motor domain signature. / Kinesin motor domain, conserved site / Kinesin motor domain ...MYCBP-associated protein / Protein FAM228 / MYCBP-associated protein family / CH-like domain in sperm protein / CH-like domain in sperm protein / Kinesin-like protein / EF-hand domain pair / Kinesin motor domain signature. / Kinesin motor domain, conserved site / Kinesin motor domain / Kinesin motor domain profile. / Kinesin motor, catalytic domain. ATPase. / Kinesin motor domain / Calponin homology domain / CH domain superfamily / Calponin homology (CH) domain profile. / Quinoprotein alcohol dehydrogenase-like superfamily / Kinesin motor domain superfamily / Tubulin-beta mRNA autoregulation signal. / Alpha tubulin / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / Tetratricopeptide-like helical domain superfamily / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / Immunoglobulin-like fold / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
WD_REPEATS_REGION domain-containing protein / Calponin-homology (CH) domain-containing protein / TPR_REGION domain-containing protein / ASH domain-containing protein / Flagellar associated protein / Uncharacterized protein / Calmodulin / Kinesin-like protein / Flagellar associated protein / Tubulin beta-1/beta-2 chain ...WD_REPEATS_REGION domain-containing protein / Calponin-homology (CH) domain-containing protein / TPR_REGION domain-containing protein / ASH domain-containing protein / Flagellar associated protein / Uncharacterized protein / Calmodulin / Kinesin-like protein / Flagellar associated protein / Tubulin beta-1/beta-2 chain / Tubulin alpha-1 chain / Flagellar WD repeat-containing protein Pf20
Similarity search - Component
Biological speciesChlamydomonas reinhardtii (plant) / Chlamydomonas smithii (plant)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.5 Å
AuthorsHan L / Zhang K
Funding support United States, 1 items
OrganizationGrant numberCountry
Other private United States
CitationJournal: Nat Struct Mol Biol / Year: 2022
Title: Cryo-EM structure of an active central apparatus.
Authors: Long Han / Qinhui Rao / Renbin Yang / Yue Wang / Pengxin Chai / Yong Xiong / Kai Zhang /
Abstract: Accurately regulated ciliary beating in time and space is critical for diverse cellular activities, which impact the survival and development of nearly all eukaryotic species. An essential beating ...Accurately regulated ciliary beating in time and space is critical for diverse cellular activities, which impact the survival and development of nearly all eukaryotic species. An essential beating regulator is the conserved central apparatus (CA) of motile cilia, composed of a pair of microtubules (C1 and C2) associated with hundreds of protein subunits per repeating unit. It is largely unclear how the CA plays its regulatory roles in ciliary motility. Here, we present high-resolution structures of Chlamydomonas reinhardtii CA by cryo-electron microscopy (cryo-EM) and its dynamic conformational behavior at multiple scales. The structures show how functionally related projection proteins of CA are clustered onto a spring-shaped scaffold of armadillo-repeat proteins, facilitated by elongated rachis-like proteins. The two halves of the CA are brought together by elastic chain-like bridge proteins to achieve coordinated activities. We captured an array of kinesin-like protein (KLP1) in two different stepping states, which are actively correlated with beating wave propagation of cilia. These findings establish a structural framework for understanding the role of the CA in cilia.
History
DepositionJun 7, 2021-
Header (metadata) releaseMay 18, 2022-
Map releaseMay 18, 2022-
UpdateJun 1, 2022-
Current statusJun 1, 2022Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_24191.png

Downloads & links

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Map

FileDownload / File: emd_24191.map.gz / Format: CCP4 / Size: 639.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationcryo-EM map of C2 of Central Pair
Voxel sizeX=Y=Z: 1.33 Å
Density
Contour LevelBy AUTHOR: 0.5
Minimum - Maximum0.0 - 9.615782
Average (Standard dev.)0.046113964 (±0.13833103)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions526606526
Spacing606526526
CellA: 805.9794 Å / B: 699.57947 Å / C: 699.57947 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: C2 microtubule map with overall mask, GSFSC resolution 3.49 angstrom.

Fileemd_24191_additional_1.map
AnnotationC2 microtubule map with overall mask, GSFSC resolution 3.49 angstrom.
Projections & Slices
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Additional map: C2 microtubule map with local mask, GSFSC resolution 3.02 angstrom.

Fileemd_24191_additional_10.map
AnnotationC2 microtubule map with local mask, GSFSC resolution 3.02 angstrom.
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Additional map: C2 microtubule map with local mask, GSFSC resolution 3.08 angstrom.

Fileemd_24191_additional_11.map
AnnotationC2 microtubule map with local mask, GSFSC resolution 3.08 angstrom.
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Additional map: C2 microtubule map with local mask, GSFSC resolution 3.12 angstrom.

Fileemd_24191_additional_12.map
AnnotationC2 microtubule map with local mask, GSFSC resolution 3.12 angstrom.
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Additional map: C2 microtubule map with local mask, GSFSC resolution 3.02 angstrom.

Fileemd_24191_additional_13.map
AnnotationC2 microtubule map with local mask, GSFSC resolution 3.02 angstrom.
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Additional map: C2 microtubule map with local mask, GSFSC resolution 3.16 angstrom.

Fileemd_24191_additional_14.map
AnnotationC2 microtubule map with local mask, GSFSC resolution 3.16 angstrom.
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Additional map: C2 microtubule map with local mask, GSFSC resolution 3.19 angstrom.

Fileemd_24191_additional_15.map
AnnotationC2 microtubule map with local mask, GSFSC resolution 3.19 angstrom.
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Additional map: C2 microtubule map with local mask, GSFSC resolution 3.07 angstrom.

Fileemd_24191_additional_16.map
AnnotationC2 microtubule map with local mask, GSFSC resolution 3.07 angstrom.
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Additional map: C2 microtubule map with local mask, GSFSC resolution 3.16 angstrom.

Fileemd_24191_additional_17.map
AnnotationC2 microtubule map with local mask, GSFSC resolution 3.16 angstrom.
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Additional map: C2 microtubule map with local mask, GSFSC resolution 3.04 angstrom.

Fileemd_24191_additional_18.map
AnnotationC2 microtubule map with local mask, GSFSC resolution 3.04 angstrom.
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Additional map: C2 microtubule map with local mask, GSFSC resolution 3.02 angstrom.

Fileemd_24191_additional_19.map
AnnotationC2 microtubule map with local mask, GSFSC resolution 3.02 angstrom.
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Additional map: C2 microtubule map with local mask, GSFSC resolution 3.07 angstrom.

Fileemd_24191_additional_2.map
AnnotationC2 microtubule map with local mask, GSFSC resolution 3.07 angstrom.
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Additional map: C2 microtubule map with local mask, GSFSC resolution 2.99 angstrom.

Fileemd_24191_additional_20.map
AnnotationC2 microtubule map with local mask, GSFSC resolution 2.99 angstrom.
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Additional map: C2 microtubule map with local mask, GSFSC resolution 2.93 angstrom.

Fileemd_24191_additional_21.map
AnnotationC2 microtubule map with local mask, GSFSC resolution 2.93 angstrom.
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Additional map: C2 microtubule map with local mask, GSFSC resolution 3.04 angstrom.

Fileemd_24191_additional_22.map
AnnotationC2 microtubule map with local mask, GSFSC resolution 3.04 angstrom.
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Additional map: KLP1 map with local mask, GSFSC resolution 3.41 angstrom.

Fileemd_24191_additional_23.map
AnnotationKLP1 map with local mask, GSFSC resolution 3.41 angstrom.
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Additional map: C2a arm map with overall mask, GSFSC resolution 4.41 angstrom.

Fileemd_24191_additional_24.map
AnnotationC2a arm map with overall mask, GSFSC resolution 4.41 angstrom.
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Additional map: C2a arm map with local mask, GSFSC resolution 3.89 angstrom.

Fileemd_24191_additional_25.map
AnnotationC2a arm map with local mask, GSFSC resolution 3.89 angstrom.
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Additional map: C2a arm map with local mask, GSFSC resolution 3.66 angstrom.

Fileemd_24191_additional_26.map
AnnotationC2a arm map with local mask, GSFSC resolution 3.66 angstrom.
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Additional map: C2a arm map with local mask, GSFSC resolution 3.63 angstrom.

Fileemd_24191_additional_27.map
AnnotationC2a arm map with local mask, GSFSC resolution 3.63 angstrom.
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Additional map: C2a arm map with local mask, GSFSC resolution 3.92 angstrom.

Fileemd_24191_additional_28.map
AnnotationC2a arm map with local mask, GSFSC resolution 3.92 angstrom.
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Additional map: C2a arm map with local mask, GSFSC resolution 3.92 angstrom.

Fileemd_24191_additional_29.map
AnnotationC2a arm map with local mask, GSFSC resolution 3.92 angstrom.
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Additional map: C2 microtubule map with local mask, GSFSC resolution 3.05 angstrom.

Fileemd_24191_additional_3.map
AnnotationC2 microtubule map with local mask, GSFSC resolution 3.05 angstrom.
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Additional map: C2a arm map with local mask, GSFSC resolution 3.87 angstrom.

Fileemd_24191_additional_30.map
AnnotationC2a arm map with local mask, GSFSC resolution 3.87 angstrom.
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Additional map: C2 microtubule map with local mask, GSFSC resolution 3.01 angstrom.

Fileemd_24191_additional_4.map
AnnotationC2 microtubule map with local mask, GSFSC resolution 3.01 angstrom.
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Additional map: C2 microtubule map with local mask, GSFSC resolution 2.99 angstrom.

Fileemd_24191_additional_5.map
AnnotationC2 microtubule map with local mask, GSFSC resolution 2.99 angstrom.
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Additional map: C2 microtubule map with local mask, GSFSC resolution 3.15 angstrom.

Fileemd_24191_additional_6.map
AnnotationC2 microtubule map with local mask, GSFSC resolution 3.15 angstrom.
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Additional map: C2 microtubule map with local mask, GSFSC resolution 3.09 angstrom.

Fileemd_24191_additional_7.map
AnnotationC2 microtubule map with local mask, GSFSC resolution 3.09 angstrom.
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Additional map: C2 microtubule map with local mask, GSFSC resolution 3.08 angstrom.

Fileemd_24191_additional_8.map
AnnotationC2 microtubule map with local mask, GSFSC resolution 3.08 angstrom.
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Additional map: C2 microtubule map with local mask, GSFSC resolution 3.09 angstrom.

Fileemd_24191_additional_9.map
AnnotationC2 microtubule map with local mask, GSFSC resolution 3.09 angstrom.
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Sample components

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Entire : Structure of C2 projections and MIPs

EntireName: Structure of C2 projections and MIPs
Components
  • Complex: Structure of C2 projections and MIPs
    • Protein or peptide: Flagellar WD repeat-containing protein Pf20
    • Protein or peptide: FAP178
    • Protein or peptide: FAP147
    • Protein or peptide: FAP239
    • Protein or peptide: FAP225
    • Protein or peptide: FAP213
    • Protein or peptide: FAP196
    • Protein or peptide: Unassigned protein-1
    • Protein or peptide: Unassigned protein-2
    • Protein or peptide: Unassigned protein-3
    • Protein or peptide: Unassigned protein-4
    • Protein or peptide: Kinesin-like protein
    • Protein or peptide: FAP65
    • Protein or peptide: FAP70
    • Protein or peptide: Unassigned protein-5
    • Protein or peptide: Unassigned protein-6
    • Protein or peptide: Tubulin beta
    • Protein or peptide: Tubulin alpha
  • Ligand: ADENOSINE-5'-DIPHOSPHATE
  • Ligand: GUANOSINE-5'-DIPHOSPHATE
  • Ligand: GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate

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Supramolecule #1: Structure of C2 projections and MIPs

SupramoleculeName: Structure of C2 projections and MIPs / type: complex / Chimera: Yes / ID: 1 / Parent: 0 / Macromolecule list: #1-#18
Source (natural)Organism: Chlamydomonas reinhardtii (plant) / Strain: CC124

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Macromolecule #1: Flagellar WD repeat-containing protein Pf20

MacromoleculeName: Flagellar WD repeat-containing protein Pf20 / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 65.929133 KDa
SequenceString: MAAAGLDTLR LEDSDDDFKY EEVDVMSDGE DDASEDLDAA LRKLQQFTSK QEAATGPART TEVKPGQVVK KPEVIDDFLR NFFIKMGLS RTCECFEAEW YELKATGRLD NSTTVPDVYL RNAELEDDVA GLRRELAEAK SIAGRASATW DKFRKERDFH R MHHKRVAQ ...String:
MAAAGLDTLR LEDSDDDFKY EEVDVMSDGE DDASEDLDAA LRKLQQFTSK QEAATGPART TEVKPGQVVK KPEVIDDFLR NFFIKMGLS RTCECFEAEW YELKATGRLD NSTTVPDVYL RNAELEDDVA GLRRELAEAK SIAGRASATW DKFRKERDFH R MHHKRVAQ EKNKLLTDLR RLKEHYAKYE PTILELKKKY ETLMKEKMMM SLERDKLAAR VDALEQVASS PLPGAERSLG GQ STAAAGG GASGRALGAT NRALTGDVPP AAGAAAAAAT GRSGAVSAGP RSGWASLNAP PRRNPYADLE FPAAPVKMLS LNK TFKGHL LSVANLALHP TKPILVTASD DKTWKMWHMP GGDLIMCGEG HKDWVAGVDF HPAGTCLASG GGDSAVKIWD FEKQ RCVTT FTDHKQAIWS VRFHHLGEVV ASGSLDHTVR LWDLPAGKCR MALRGHVDSV NDLAWQPFSS SLATASSDKT VSVWD ARAG LCTQTYYGHQ NSCNGVSFNI LGTQLASTDA DGVVKLWDTR MTAEVATINT GKHPANKSCF DRSGQVLAVA CDDGKV KAY STTDGVLQAE LAGHEDAVQA VLFDPAGQYL VSCGSDNTFR LWS

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Macromolecule #2: FAP178

MacromoleculeName: FAP178 / type: protein_or_peptide / ID: 2 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 23.511406 KDa
SequenceString: MTNRATLSQT ATSLTSTTGR RDRLQRQEDT TLFGYESPPD TPALHRDVLK WVQGLDLSQS LKNCRRDVAN GFLVAEIFSR YFPADIQMH SFANAASSHF KRDNWTQLQA FCGRQGINLP GDLVEGCVQG VHGAAIALLE HLYEAFTGKK VPRLKVPDAA A AAAAAAAA ...String:
MTNRATLSQT ATSLTSTTGR RDRLQRQEDT TLFGYESPPD TPALHRDVLK WVQGLDLSQS LKNCRRDVAN GFLVAEIFSR YFPADIQMH SFANAASSHF KRDNWTQLQA FCGRQGINLP GDLVEGCVQG VHGAAIALLE HLYEAFTGKK VPRLKVPDAA A AAAAAAAA RGGPGAVGGS GEGAKLISST VKTAQNLEFG AVTTQQLAGA DATALRRRLA GGTS

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Macromolecule #3: FAP147

MacromoleculeName: FAP147 / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 102.141609 KDa
SequenceString: MVGPSEPAGL PPKAKLKAYK VLGDVGEFQE RLAATLPPPK APEPPNVFNI SDTSEFPPDV WFAATISPDN GVAVIASYRE LCKARALYQ SQLTNIHTAR RQYGMGGSMG PGAGAPLPLP PAQQAAAENR TIAETMRVAA DTRKLGKFEQ QQAKWDEVSS T LAYRVGRA ...String:
MVGPSEPAGL PPKAKLKAYK VLGDVGEFQE RLAATLPPPK APEPPNVFNI SDTSEFPPDV WFAATISPDN GVAVIASYRE LCKARALYQ SQLTNIHTAR RQYGMGGSMG PGAGAPLPLP PAQQAAAENR TIAETMRVAA DTRKLGKFEQ QQAKWDEVSS T LAYRVGRA PTELAMQRGP AWRTRAELTE LLYRAQPRDA RGSNPDEVWT ASLRDAWERI LPLGSIFSGL AIKIRDRPGE LP ATRAARV GRPLDPLLAP LGGGGTTLSP ATLGHPAAAA AHAQHVATLA ANGVMLGATT NKPPLGRSLS ARGRAWEDSE MLK QRVAEY GTRLRALAPH DPDFGALVVA GEALESQLEA LAGAPITLAE VESHVRGHAP EAFEAYKAVK DEMEAQLRAA LEAE EAQRA AQEAAAAAAA PQPGPHVAFS SPFVSLACHV GEKAHGSVTL VSRGTAAVNW SWRRVPAPQH AHAATELSQP PCFAA SLQS GVLLPGQSLT VAVTFEAAAA GTYREAWELV TRPPLQGSEG PCLTLRLRGA AEVRDESGTG RGALEEALAE KEKRAK VAA ALERVLRDVR MPRRPQPHES VEELAAGDAW DRVNGCAAGA VGGPYAWPPL FHSPGVQSEL AEVYREAEAA LAAALKP SV DEDPKKKKKE TSARKGKKGD EPPPPPPKYP PSWTEPAPAG AGCSLAVLDE LLTELAAAAP AAAAPLAVRA EEAKARAR V PPNNRKVLRR AMRVLVGKMV DAVEEKFNVI RTDLEAKSAA EAAAAAAAAA AAAGEDGDDA DGEASAISVG AVKEKDKEK EKDGKEKKGG AKEKPGAKGG AGKDKKGGSA SHTDDGAAPE PGAGLPNVEH LQPVFHDKAT TAAKAAIKAA LNTLLDEVHR HKVAAGDAL EANIRSLDSR IEAAQADAQA NQEPAAAEAA AAAGLALFAP AAGGVPAPGP GPAARARSAE LEQLYWERLC V LRTWTGLG IPTRADVR

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Macromolecule #4: FAP239

MacromoleculeName: FAP239 / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 57.734598 KDa
SequenceString: MPPQLGREVQ ERVKVYGPLN ELTYEGRLLT QTLQDELNRS ISAPAGPRSP WYEGDPELES MRERVRQQRA IREAQRRRDH AALTASIQK RNLQEEQRRD AMLGSLLGDV IGGLTDPNSP LAEAEAALSH ADKVRRKKKE SLHNEWSTQV FDTIQGRLQA A VDARDPAA ...String:
MPPQLGREVQ ERVKVYGPLN ELTYEGRLLT QTLQDELNRS ISAPAGPRSP WYEGDPELES MRERVRQQRA IREAQRRRDH AALTASIQK RNLQEEQRRD AMLGSLLGDV IGGLTDPNSP LAEAEAALSH ADKVRRKKKE SLHNEWSTQV FDTIQGRLQA A VDARDPAA IESRLKTQYD QYLHTTNTKV AVFRDVIIEQ DYNPLAAADA AIRVPTGDIR DPLKRDVLKG EYERRLMTGG RG GGGASPT GRGGAAAAGA GSIYGPLGKE TLGTQQWGEL AVKATPYGHC TDGQGGYVAR PLSGSAVALR ASRVPMDHYD YPV GNAAAA AEVPPGKRIV PGPEQRRGRQ DLFDVVQHTV HLKPQGYTGG DQWLEHKGKG NAPGPEQRRG RRDLADVLQQ KAVA DGPRG TSAPARGDQL QHKEQGDAWL DAKGKRRVEG PEMRRGRQGL YETLQQTSNP YQGGNKVGDA WLEHKGRKVQ PRPEP EAAA ALSAVPPLPT VRPPRVGDDK KYAVNIEAAM GQMTVKDGAK VTGW

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Macromolecule #5: FAP225

MacromoleculeName: FAP225 / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 81.430711 KDa
SequenceString: MATYEPPRSP GSRRVRRHAM GVSNASSIDE CEASSSARST VTLIQSGRLV RLQPHERPTD SVARETRTED RPIVDKVHDK LFKAHRERF VHKVLRSYAQ DDSGLLTPDQ LRSALDRLHT GLDAAEKDRI VARVAPAQHG KVHYMDFIRS LESPQPLGGP G LGVGFPGV ...String:
MATYEPPRSP GSRRVRRHAM GVSNASSIDE CEASSSARST VTLIQSGRLV RLQPHERPTD SVARETRTED RPIVDKVHDK LFKAHRERF VHKVLRSYAQ DDSGLLTPDQ LRSALDRLHT GLDAAEKDRI VARVAPAQHG KVHYMDFIRS LESPQPLGGP G LGVGFPGV TPQRAAAAGT APTFWNWQRH KKQHVPGLLE EVREGTFEQA QSDALLTSLM STKLNQYRDK LRLIFRQMDG NR NSLIDRE EFIRGIAKLR INVSKAQVER LFDLCDVDKS GELDYEEFVN RFEENGLASA ARNQTRAQPQ QPDGAPATVP LAQ SLGLTQ DEVAGALSHP MVHELARSLY GKASGATSVF VRNDLTRCGQ LPVRDMTRCC QALVPGISER QVAAVMAVVD PNSA GGVDY RAFVQKLTET GVANPRMLHS APARGEGFTA TGALTRFGEG GSLTAPDALP SAGSRSLSAV GTCRSPGGSG TTVLP ISAG LGVVLQPGGT LPPGAVTTRP AADRTSLDDL HDVCVAPFLE SLSRAGNNDL PAQHDNNNNN GGAGGPATSL PSVTRS IDM GTLSRSASLP NAHGGSPTRF GGAGGGGGFG GTAAGLSATG SKAPTHATGR FSRFWDRRYA DTSHITSVDP CSASYAP SE GTYVRKGWGS GDASSDFLTY QGADRDQRAR QRQAVAVRTT ARSEVEAKLS GLDDASGLDD GRLQVARATK QRYEERAE M YDRTRQQHEG GSCIFGRLPP FHEHQLEAAN TPARVFW

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Macromolecule #6: FAP213

MacromoleculeName: FAP213 / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 22.262674 KDa
SequenceString: MEGAAGPSGF RNVEPLSRQE RAAARDKDLL EKSRLQARNR GGPLKQPENV VGNPVMPARN APAFCDEYDR FNRDVAGEMN AKKQQNLQK KEEVYAVKRA EQYHRERSNW ETQAQAAARE AARLEASRTT GTGAKRNQGS ESYNIISLNY NNSSGGQQLA A KDTAVKEA ...String:
MEGAAGPSGF RNVEPLSRQE RAAARDKDLL EKSRLQARNR GGPLKQPENV VGNPVMPARN APAFCDEYDR FNRDVAGEMN AKKQQNLQK KEEVYAVKRA EQYHRERSNW ETQAQAAARE AARLEASRTT GTGAKRNQGS ESYNIISLNY NNSSGGQQLA A KDTAVKEA RQARAVNLYS KSHSVSHNII TGEPIKFPTA GKE

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Macromolecule #7: FAP196

MacromoleculeName: FAP196 / type: protein_or_peptide / ID: 7 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 64.880324 KDa
SequenceString: MPLVLRHIAG LNGQMFGAMR SIPNSRGPYN IAQISGVSVI LGNENNLAPQ FTLRGHDDVI TAFAMSHGGS MLATGQKGSN SDVIVWDVA ACAPKYKFQE HDVEVATVSF SADDRLLLSA SCFTDAKLYV WDMATGKIVA KHGLEPGKRI TCSAWSPALA N GRAYTCAT ...String:
MPLVLRHIAG LNGQMFGAMR SIPNSRGPYN IAQISGVSVI LGNENNLAPQ FTLRGHDDVI TAFAMSHGGS MLATGQKGSN SDVIVWDVA ACAPKYKFQE HDVEVATVSF SADDRLLLSA SCFTDAKLYV WDMATGKIVA KHGLEPGKRI TCSAWSPALA N GRAYTCAT AAGEELALWT VDPFTGMVSG QKVVLGTVRR EYTSLAFSAD GAWLYGGTTS ADVVTVNVQR ASVQMTHPVC SG GVGALLL SPDGGGACLV GGRDGSLSTF NAAGGAWREL RPFAKVPGCV TSLSLSADGG AFLVGTAQGG VYRLDRTSLQ LHT ISQAQQ GQLTAVAFAP GQPEAVATSS TDGSIYVWST ADYTLQCRVQ EPTQAGGALC CVLTRDAVIS GWGDGNIRCH ARAA GGATC AQMWMIPGAH ALAHSVGVTA LRLSNGGAFL LSGGMGGELR VWDLRSREMA AHMKMHNSRI VGAAVMADDK HVAAA SEDR TWSLWDVGSE RVRTTWRGQS MFRGMAVSAD KVSVVTVTLD RKIQMWDVRG PDPRWTKVEA HGKEVGCVAS DHRGAA FAT GGADQAVRVW DWRNGGEVGG AACHSAPVAA MAFSPDDAGL VSVALDGSTC FWQLT

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Macromolecule #8: Unassigned protein-1

MacromoleculeName: Unassigned protein-1 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant) / Strain: CC124
Molecular weightTheoretical: 14.400733 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #9: Unassigned protein-2

MacromoleculeName: Unassigned protein-2 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant) / Strain: CC124
Molecular weightTheoretical: 14.485838 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #10: Unassigned protein-3

MacromoleculeName: Unassigned protein-3 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant) / Strain: CC124
Molecular weightTheoretical: 13.634798 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #11: Unassigned protein-4

MacromoleculeName: Unassigned protein-4 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant) / Strain: CC124
Molecular weightTheoretical: 3.337105 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)

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Macromolecule #12: Kinesin-like protein

MacromoleculeName: Kinesin-like protein / type: protein_or_peptide / ID: 12 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 83.108039 KDa
SequenceString: MVKQAVKVFV RTRPTATSGS GLKLGPDGQS VSVNVPKDLS AGPVNNQQEQ FSFKFDGVLE NVSQEAAYTT LAHEVVDSLM AGYNGTIFA YGQTGAGKTF TMSGGGTAYA HRGLIPRAIH HVFREVDMRA DKMYRVHVSY LEIYNEQLYD LLGDTPGTSD A LAVLEDSN ...String:
MVKQAVKVFV RTRPTATSGS GLKLGPDGQS VSVNVPKDLS AGPVNNQQEQ FSFKFDGVLE NVSQEAAYTT LAHEVVDSLM AGYNGTIFA YGQTGAGKTF TMSGGGTAYA HRGLIPRAIH HVFREVDMRA DKMYRVHVSY LEIYNEQLYD LLGDTPGTSD A LAVLEDSN SNTYVRGLTL VPVRSEEEAL AQFFLGEQGR TTAGHVLNAE SSRSHTVFTI HVEMRTSDAA SERAVLSKLN LV DLAGSER TKKTGVTGQT LKEAQFINRS LSFLEQTVNA LSRKDTYVPF RQTKLTAVLR DALGGNCKTV MVANIWAEPS HNE ETLSTL RFASRVRTLT TDLALNESND PALLLRRYER QIKELKAELA MRDTLSGKGR VSYDDLTDDE LRELHATCRR FLHG EAEPE DLPADSMKRV RETFKALRAV HVAIKADMAT QMATLRRATE EGSGAAARGG DSAGPSGVGD VDLRATGGFT VGHAP LDAR PPVRSELGSP GAGASGAEAL GEPRSPGGGL HAQASSHTDA GSNWGDAGPL SSPGGTRLAG IFGVSGDRNA VFRRYK VDV GEGRELAASL KAASIALADT KASIRSLGAS VNDAKQRIDE LSSALALRRG ATPAGGDGEV LDSEAYALMQ ELKSAKS RY RTDFDSLKSA REELEPQIQA VAVARAGLLE AFDRWAAAQS DTTLKRMATA GRAMSGIAPG EEDEMDAGEQ FERMQIAR I SERDPDSLAF HTALKRTGAA VSRPATVATG GNAKAAAMAT RKMEHTQAVN RGLAR

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Macromolecule #13: FAP65

MacromoleculeName: FAP65 / type: protein_or_peptide / ID: 13 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 233.221547 KDa
SequenceString: MPASNGADQG TAPGGTMAYG VLSREQRKQM FGLDCPEQLE WRHWNPGTEY VKQLVLKNVS TSVLKIRFKQ PTSKAFGMDF PEPFKLRPG MSQPLKVVFR PLKQQHYSDN VELFVGNVSC LVPVHAYTPV THIEVPPALD FGFTPTKETV TAQLPVRNTG D VRVDVLWK ...String:
MPASNGADQG TAPGGTMAYG VLSREQRKQM FGLDCPEQLE WRHWNPGTEY VKQLVLKNVS TSVLKIRFKQ PTSKAFGMDF PEPFKLRPG MSQPLKVVFR PLKQQHYSDN VELFVGNVSC LVPVHAYTPV THIEVPPALD FGFTPTKETV TAQLPVRNTG D VRVDVLWK LDAPFSISPL FASLAPGEVV AFTASFTPPE ACSYTASAAC QLESGAAAIC KISGIGKFPY LSVEQAGVDF GA VVVGQRV ERLVRFGNHS VVPAHFAVTH DEAGPDDGVF TVGPARGTLG PEEYSMLKLS YTPRATGTFS SETFHIGTAG GNR VALNLR GTAVAPLVTL STRAFNFGNV AAGATASRVL YIRNHSAVPV PYDFQVDPLD VFAISRTRGV LAPDSTAHVT ITFR PMAGL AANLWRRVTL LLKDAEPQGV DLVATAYTDK SRPPPLSQRH VERYMARVLS GGPAVEESQL DSKPPSAATG AGGLD GASE GGAGPLLAGG GFGSLLPPLG DAAEGGEGAA SKLLGPDGWS LLFGGQDPAA ALTVDSDALD FGSCSRLSAS EYRSVT VTN HTPAKLTAFV LVPEWADPGA APGDAPQRVF QVFPESADMR PYGQATFKVA FRPPRDAAFF SQQLALVAHT KSQRNFR LV AEHQVLPAWC VPLRATGNTF LHSNPEFGPK VELSSRAVAF PPCRPGERVH QTLLIANSGD TPVAFSFGAA GAALGPLF S AKPAAGVVPP QGHVLVALRF APTDTRPAAA NALVTFNGVA SNAVSVSLRG AAHTPRLTTD LPSSTLYFRP TCVGASSQR AVTLHNPSRV PVAFRWRLPA RLQGLVTVSP AAGALRGNES VQLQWSFTPA AQKLYEARAA CLVMSPPEAA NGNAAGATAS AASLFPGAG LATGIEWYEG AGATAALAGM GEEEAADAVL LSLVGEGTQG AVALEPPSLE LGDLRVGHPV RRPLLLQNCS D GVLRYSLE VGPAEDDQPP AGDATLVDFT AGLDSAAEGG VGGGAGGAGG AALECWVDEP EGALPARASK TVMVTLFPRY RK RYSLQVR CRSSTVAPLL TGPNRPPSGG AGPRPLDAGS EAAAAAAAAA AAAAAPLPPP VIAPLTASTT FPTLEVTDVY CEG LPKQLL WQLLGLNDLN HHLRTEVTAT ELRLRAAQDR GALTTEAASA AMRPFLMEFG THGLGGRPRV VHVEISNPTP LPAS WQLHS FDDPDGVELE NWVEPGRPRT EGERMRDLIA EYKLFEMRPR SGELEPGARC TVTIEFRPSV EGSFELPVFL HITDG KRLR LQLQAVTTPE PLQLLALPPP LRTFRLEPVA LGERAPPLQM YVLRNGGPAP LHWRLDTAPL AALAEASWGH PVLELV GPE EGDIEEGGVA AINWRFSPLE AKEYRVEVPV LLGDGGIEVI ELLGRGFAPP PAPPHGAAAV QLIGPGAATS PAAPTTA AE DDDTPAAALD SVGGDAEAEA ARAAAAADRD WITWRGLSSA PSAGMAGRLA LVDHDLVSLG VTPVRGLTRR IIVLTNKS R YPLAFDWDLG CLAPPPPPGL AATQRAAAAS AMMGLGGGSL LPASQLQLLA GRPLQGALAI SPAAGSLEPG ERLVCRVSL HAGVTPQVFE GEVRCHVRID DDAVAEAEAA AAAAAAAGAA AVAAELPFVE QVEEVIAEAP VRGPPAPPPA VAAAQRASRL RSRLPVHQY MTTAVRTRIE PLNAAFTATM EARTRRLADA TRPPSWPEPQ SISVTLRGRI LDERQLGALR YVPPHERAAA R AAVVAGAA WVPPAMVPFW EEEGRSTPAV SAQPSSQGGG GYGVGGYGGG YGSGGSQGGE ASVAPSGGDP GGYSPRGGMA DS WPSLEPG LGMEETVVGP PTVGLDLSLS LQHAGGAGGA PGGDGDSDSR PGTPSMTAAA HHHHHHPRHA GPAPGSGTAE VME VTFSGD QQPTGGPMPS PPSISGAPDP DSATPDDDFA TSDAAVNAAA AVRAAGGGMG SPQPVDPGGS AAAAASELAP PALP PAPPR GGDIEYDALV SAREVLAGLF AELMEDADVV RAVAFLPEHE VPSFAEVRAT PPPCYDPAVR AAAEAARAEA EARAA AEAA TKAAAEAEAV AARAAAMLGP KHEASSSSSS SSSSSSSSSS SSSSSSAASS PGASPTGKAP VVEAATPRLV SAVGGM APS QPALALRPAP STPPRTPPKP SQGAAFAIPA AAAQAATEPP ALEDELAADT ELARVLVAPE WRAFADFVLE SAVLGLM QE SAAGDWEMPE EEVGR

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Macromolecule #14: FAP70

MacromoleculeName: FAP70 / type: protein_or_peptide / ID: 14 / Number of copies: 4 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 111.116258 KDa
SequenceString: MAPKKKEDKD APPPEDTNPY GVFVSVTMPS ATVVLDVQDD AAAQRETHIT LNIPGCHVPW ASAHTAPSEG GVYQYSVVKH FRRSGGQDG LLQLINGVLT VTVNDSATNA VLASATVDQL QGFAIGQNTW STEGLDLVPA AEVPEGVPKA RSARLAFASI A LQERPLPE ...String:
MAPKKKEDKD APPPEDTNPY GVFVSVTMPS ATVVLDVQDD AAAQRETHIT LNIPGCHVPW ASAHTAPSEG GVYQYSVVKH FRRSGGQDG LLQLINGVLT VTVNDSATNA VLASATVDQL QGFAIGQNTW STEGLDLVPA AEVPEGVPKA RSARLAFASI A LQERPLPE GADAAALAAA PPPPEAELPP LLPYSYVSGE AAEKGNMVEL VVSGLSPLPP NLQAAADAAG GKLHVTVGLA LP GGGPAVA VSLAVAGGRV AAPAFRRELL PPGCLQALQY ALEDGLPVVL EVARYVSAEG MADPAFEGYH AAAAAPALAA GLG AAGATE AAAEGLSLTA WAASGAKTCL PPYTAPAGKP KPVEAEVPPA GTCLWEKAGA QLAVSLRFAR PVVPAWRPPP PPPR PLLEL IPPRDLTPKP PPTTAVDEFK AKVRVIARAL AEEYKAVLPP PDASVAAAAA GGGAEGRHKA LIFELNRSGK YAQMR DSLK TAVVSLVREK YRKSGSMSPN EMALLYNDLY GSLLAAVHSS LNDLVDAAAA RPRAPPPAPV PDKQRLGELL ELAAQA EAM GDTDRAELLH QRRLLAKNDA QVWYEYGTYC LRRGGAKRGR AEECFREALA LEPAHRGALL ALLGCSVAAG RNTDPAY LE SAEAAAHRLL DVAGRSSLDA WAALAVVYRA YGEAKRAELA SCEQEMARLE KQQLAAAAAA ASAGVSPSPS YGEGRAGG N GGGVPATEPS TASAGSVAGS AGELQARSFI SLANTLLESL ALPAEAALAL ELAAGLRHWP SVGPDTRTLH ALAGALAEQ ALARAAGGGA ASAAAEAMLT PGSSVLSMMR ADAGEAVSSV AAEAAWRCRL LVAQLHKARG ATDEAIRFYQ EYIEAARSSG RLAEVPLSA WLELAEAYAA RGQARFAADV FLLGASARPG CAVLWRGAGR CFVGAEELGP ADMALSEANV LDPEDPEAWG W LALVALRE GRAEDAEKAL AFGLRCGLGD PGLLLDIAAE YRAAGQRRAE QRVLQEVAVK LMPESCSARL LLARCLVAQR CG AEAAEAV AAARQLAAHE DDEAAVAELE AELRGMA

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Macromolecule #15: Unassigned protein-5

MacromoleculeName: Unassigned protein-5 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant) / Strain: CC124
Molecular weightTheoretical: 7.507245 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

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Macromolecule #16: Unassigned protein-6

MacromoleculeName: Unassigned protein-6 / type: protein_or_peptide / ID: 16 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas smithii (plant) / Strain: CC124
Molecular weightTheoretical: 14.826253 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

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Macromolecule #17: Tubulin beta

MacromoleculeName: Tubulin beta / type: protein_or_peptide / ID: 17 / Number of copies: 52 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 49.665809 KDa
SequenceString: MREIVHIQGG QCGNQIGAKF WEVVSDEHGI DPTGTYHGDS DLQLERINVY FNEATGGRYV PRAILMDLEP GTMDSVRSGP YGQIFRPDN FVFGQTGAGN NWAKGHYTEG AELIDSVLDV VRKEAESCDC LQGFQVCHSL GGGTGSGMGT LLISKIREEY P DRMMLTFS ...String:
MREIVHIQGG QCGNQIGAKF WEVVSDEHGI DPTGTYHGDS DLQLERINVY FNEATGGRYV PRAILMDLEP GTMDSVRSGP YGQIFRPDN FVFGQTGAGN NWAKGHYTEG AELIDSVLDV VRKEAESCDC LQGFQVCHSL GGGTGSGMGT LLISKIREEY P DRMMLTFS VVPSPKVSDT VVEPYNATLS VHQLVENADE CMVLDNEALY DICFRTLKLT TPTFGDLNHL ISAVMSGITC CL RFPGQLN ADLRKLAVNL IPFPRLHFFM VGFTPLTSRG SQQYRALTVP ELTQQMWDAK NMMCAADPRH GRYLTASALF RGR MSTKEV DEQMLNVQNK NSSYFVEWIP NNVKSSVCDI PPKGLKMSAT FIGNSTAIQE MFKRVSEQFT AMFRRKAFLH WYTG EGMDE MEFTEAESNM NDLVSEYQQY QDASAEEEGE FEGEEEEA

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Macromolecule #18: Tubulin alpha

MacromoleculeName: Tubulin alpha / type: protein_or_peptide / ID: 18 / Number of copies: 52 / Enantiomer: LEVO
Source (natural)Organism: Chlamydomonas reinhardtii (plant)
Molecular weightTheoretical: 49.638008 KDa
SequenceString: MREVISIHIG QAGIQVGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDAFN TFFSETGAGK HVPRCIFLDL EPTVVDEVRT GTYRQLFHP EQLISGKEDA ANNFARGHYT IGKEIVDLAL DRIRKLADNC TGLQGFLVFN AVGGGTGSGL GSLLLERLSV D YGKKSKLG ...String:
MREVISIHIG QAGIQVGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDAFN TFFSETGAGK HVPRCIFLDL EPTVVDEVRT GTYRQLFHP EQLISGKEDA ANNFARGHYT IGKEIVDLAL DRIRKLADNC TGLQGFLVFN AVGGGTGSGL GSLLLERLSV D YGKKSKLG FTVYPSPQVS TAVVEPYNSV LSTHSLLEHT DVAVMLDNEA IYDICRRSLD IERPTYTNLN RLIAQVISSL TA SLRFDGA LNVDITEFQT NLVPYPRIHF MLSSYAPIIS AEKAYHEQLS VAEITNAAFE PASMMVKCDP RHGKYMACCL MYR GDVVPK DVNASVATIK TKRTIQFVDW CPTGFKCGIN YQPPTVVPGG DLAKVQRAVC MISNSTAIGE IFSRLDHKFD LMYA KRAFV HWYVGEGMEE GEFSEAREDL AALEKDFEEV GAESAEGAGE GEGEEY

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Macromolecule #19: ADENOSINE-5'-DIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 19 / Number of copies: 4 / Formula: ADP
Molecular weightTheoretical: 427.201 Da
Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

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Macromolecule #20: GUANOSINE-5'-DIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 20 / Number of copies: 52 / Formula: GDP
Molecular weightTheoretical: 443.201 Da
Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

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Macromolecule #21: GUANOSINE-5'-TRIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 21 / Number of copies: 52 / Formula: GTP
Molecular weightTheoretical: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statefilament

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 100.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.2 µm
Image recordingFilm or detector model: GATAN K2 QUANTUM (4k x 4k) / Average electron dose: 38.6 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 192253

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