- EMDB-24191: structure of C2 projections and MIPs -
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基本情報
登録情報
データベース: EMDB / ID: EMD-24191
タイトル
structure of C2 projections and MIPs
マップデータ
cryo-EM map of C2 of Central Pair
試料
複合体: Structure of C2 projections and MIPs
タンパク質・ペプチド: x 18種
リガンド: x 3種
キーワード
central pair apparatus / C2 projection / kinesin motor protein / STRUCTURAL PROTEIN
機能・相同性
機能・相同性情報
cilium movement / kinesin complex / microtubule motor activity / motile cilium / microtubule-based movement / regulation of cytoskeleton organization / axoneme / cilium assembly / microtubule-based process / structural constituent of cytoskeleton ...cilium movement / kinesin complex / microtubule motor activity / motile cilium / microtubule-based movement / regulation of cytoskeleton organization / axoneme / cilium assembly / microtubule-based process / structural constituent of cytoskeleton / microtubule binding / 加水分解酵素; 酸無水物に作用; GTPに作用・細胞または細胞小器官の運動に関与 / microtubule / hydrolase activity / cilium / GTPase activity / calcium ion binding / GTP binding / ATP hydrolysis activity / ATP binding / metal ion binding / cytoplasm 類似検索 - 分子機能
MYCBP-associated protein / Protein FAM228 / : / : / : / : / MYCBP-associated protein family / Cilia- and flagella-associated protein 65-like, Ig-like domain / Cilia- and flagella-associated protein 65-like, Ig-like domain / : ...MYCBP-associated protein / Protein FAM228 / : / : / : / : / MYCBP-associated protein family / Cilia- and flagella-associated protein 65-like, Ig-like domain / Cilia- and flagella-associated protein 65-like, Ig-like domain / : / : / CFAP74 first Ig-like domain / CH-like domain in sperm protein / CH-like domain in sperm protein / CFAP65, fourth Ig-like domain / : / : / Kinesin-like protein / Kinesin motor domain signature. / Kinesin motor domain, conserved site / Kinesin motor domain / Kinesin motor domain profile. / Kinesin motor, catalytic domain. ATPase. / Kinesin motor domain / Calponin homology domain / CH domain superfamily / Calponin homology (CH) domain profile. / Quinoprotein alcohol dehydrogenase-like superfamily / Kinesin motor domain superfamily / Tubulin-beta mRNA autoregulation signal. / Alpha tubulin / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / Tetratricopeptide-like helical domain superfamily / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / WD domain, G-beta repeat / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / Immunoglobulin-like fold / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性
Uncharacterized protein / Calponin-homology (CH) domain-containing protein / Cilia- and flagella-associated protein 70 / Abnormal spindle-like microcephaly-associated protein ASH domain-containing protein / Flagellar associated protein / Flagellar associated protein / EF-hand domain-containing protein / Kinesin-like protein / Flagellar associated protein / Tubulin beta-1/beta-2 chain ...Uncharacterized protein / Calponin-homology (CH) domain-containing protein / Cilia- and flagella-associated protein 70 / Abnormal spindle-like microcephaly-associated protein ASH domain-containing protein / Flagellar associated protein / Flagellar associated protein / EF-hand domain-containing protein / Kinesin-like protein / Flagellar associated protein / Tubulin beta-1/beta-2 chain / Tubulin alpha-1 chain / Flagellar WD repeat-containing protein Pf20 類似検索 - 構成要素
ジャーナル: Nat Struct Mol Biol / 年: 2022 タイトル: Cryo-EM structure of an active central apparatus. 著者: Long Han / Qinhui Rao / Renbin Yang / Yue Wang / Pengxin Chai / Yong Xiong / Kai Zhang / 要旨: Accurately regulated ciliary beating in time and space is critical for diverse cellular activities, which impact the survival and development of nearly all eukaryotic species. An essential beating ...Accurately regulated ciliary beating in time and space is critical for diverse cellular activities, which impact the survival and development of nearly all eukaryotic species. An essential beating regulator is the conserved central apparatus (CA) of motile cilia, composed of a pair of microtubules (C1 and C2) associated with hundreds of protein subunits per repeating unit. It is largely unclear how the CA plays its regulatory roles in ciliary motility. Here, we present high-resolution structures of Chlamydomonas reinhardtii CA by cryo-electron microscopy (cryo-EM) and its dynamic conformational behavior at multiple scales. The structures show how functionally related projection proteins of CA are clustered onto a spring-shaped scaffold of armadillo-repeat proteins, facilitated by elongated rachis-like proteins. The two halves of the CA are brought together by elastic chain-like bridge proteins to achieve coordinated activities. We captured an array of kinesin-like protein (KLP1) in two different stepping states, which are actively correlated with beating wave propagation of cilia. These findings establish a structural framework for understanding the role of the CA in cilia.
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機能・相同性情報
Chlamydomonas reinhardtii (クラミドモナス)
データ登録者
米国, 1件 
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