- EMDB-24191: structure of C2 projections and MIPs -
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Basic information
Entry
Database: EMDB / ID: EMD-24191
Title
structure of C2 projections and MIPs
Map data
cryo-EM map of C2 of Central Pair
Sample
Complex: Structure of C2 projections and MIPs
Protein or peptide: x 18 types
Ligand: x 3 types
Keywords
central pair apparatus / C2 projection / kinesin motor protein / STRUCTURAL PROTEIN
Function / homology
Function and homology information
axonemal central apparatus / sperm axoneme assembly / cilium movement / axoneme assembly / motile cilium / microtubule motor activity / kinesin complex / microtubule-based movement / regulation of cytoskeleton organization / axoneme ...axonemal central apparatus / sperm axoneme assembly / cilium movement / axoneme assembly / motile cilium / microtubule motor activity / kinesin complex / microtubule-based movement / regulation of cytoskeleton organization / axoneme / cilium assembly / sperm flagellum / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / structural constituent of cytoskeleton / microtubule cytoskeleton organization / mitotic cell cycle / microtubule binding / microtubule / hydrolase activity / GTPase activity / calcium ion binding / GTP binding / ATP hydrolysis activity / extracellular exosome / ATP binding / metal ion binding / cytoplasm Similarity search - Function
MYCBP-associated protein / Protein FAM228 / MYCBP-associated protein family / Flagellar-associated PapD-like / CH-like domain in sperm protein / CH-like domain in sperm protein / Kinesin-like protein / Kinesin motor domain signature. / Kinesin motor domain, conserved site / Kinesin motor domain ...MYCBP-associated protein / Protein FAM228 / MYCBP-associated protein family / Flagellar-associated PapD-like / CH-like domain in sperm protein / CH-like domain in sperm protein / Kinesin-like protein / Kinesin motor domain signature. / Kinesin motor domain, conserved site / Kinesin motor domain / Kinesin motor domain profile. / Kinesin motor, catalytic domain. ATPase. / Kinesin motor domain / Calponin homology domain / CH domain superfamily / Calponin homology (CH) domain profile. / Quinoprotein alcohol dehydrogenase-like superfamily / Kinesin motor domain superfamily / Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / Tetratricopeptide-like helical domain superfamily / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / Immunoglobulin-like fold / P-loop containing nucleoside triphosphate hydrolase Similarity search - Domain/homology
Uncharacterized protein / Calponin-homology (CH) domain-containing protein / Cilia- and flagella-associated protein 70 / Abnormal spindle-like microcephaly-associated protein ASH domain-containing protein / Flagellar associated protein / Flagellar associated protein / EF-hand domain-containing protein / Kinesin-like protein / Flagellar associated protein / Tubulin beta-1/beta-2 chain ...Uncharacterized protein / Calponin-homology (CH) domain-containing protein / Cilia- and flagella-associated protein 70 / Abnormal spindle-like microcephaly-associated protein ASH domain-containing protein / Flagellar associated protein / Flagellar associated protein / EF-hand domain-containing protein / Kinesin-like protein / Flagellar associated protein / Tubulin beta-1/beta-2 chain / Tubulin alpha-1 chain / Flagellar WD repeat-containing protein Pf20 Similarity search - Component
Biological species
Chlamydomonas reinhardtii (plant)
Method
single particle reconstruction / cryo EM / Resolution: 3.5 Å
Journal: Nat Struct Mol Biol / Year: 2022 Title: Cryo-EM structure of an active central apparatus. Authors: Long Han / Qinhui Rao / Renbin Yang / Yue Wang / Pengxin Chai / Yong Xiong / Kai Zhang / Abstract: Accurately regulated ciliary beating in time and space is critical for diverse cellular activities, which impact the survival and development of nearly all eukaryotic species. An essential beating ...Accurately regulated ciliary beating in time and space is critical for diverse cellular activities, which impact the survival and development of nearly all eukaryotic species. An essential beating regulator is the conserved central apparatus (CA) of motile cilia, composed of a pair of microtubules (C1 and C2) associated with hundreds of protein subunits per repeating unit. It is largely unclear how the CA plays its regulatory roles in ciliary motility. Here, we present high-resolution structures of Chlamydomonas reinhardtii CA by cryo-electron microscopy (cryo-EM) and its dynamic conformational behavior at multiple scales. The structures show how functionally related projection proteins of CA are clustered onto a spring-shaped scaffold of armadillo-repeat proteins, facilitated by elongated rachis-like proteins. The two halves of the CA are brought together by elastic chain-like bridge proteins to achieve coordinated activities. We captured an array of kinesin-like protein (KLP1) in two different stepping states, which are actively correlated with beating wave propagation of cilia. These findings establish a structural framework for understanding the role of the CA in cilia.
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