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- EMDB-18999: Cryo-EM structure of coagulation factor beta-XIIa in complex with... -
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Open data
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Basic information
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Title | Cryo-EM structure of coagulation factor beta-XIIa in complex with the garadacimab Fab fragment (symmetric dimer) | |||||||||
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![]() | Complex / coagulation / trypsin-like serine protease / hereditary angioedema (HAE) / BLOOD CLOTTING | |||||||||
Function / homology | ![]() coagulation factor XIIa / plasma kallikrein-kinin cascade / Factor XII activation / Defective SERPING1 causes hereditary angioedema / response to misfolded protein / positive regulation of plasminogen activation / blood coagulation, intrinsic pathway / positive regulation of fibrinolysis / misfolded protein binding / zymogen activation ...coagulation factor XIIa / plasma kallikrein-kinin cascade / Factor XII activation / Defective SERPING1 causes hereditary angioedema / response to misfolded protein / positive regulation of plasminogen activation / blood coagulation, intrinsic pathway / positive regulation of fibrinolysis / misfolded protein binding / zymogen activation / Defective factor XII causes hereditary angioedema / protein autoprocessing / positive regulation of blood coagulation / rough endoplasmic reticulum / fibrinolysis / Intrinsic Pathway of Fibrin Clot Formation / protein processing / blood coagulation / collagen-containing extracellular matrix / serine-type endopeptidase activity / innate immune response / calcium ion binding / extracellular space / extracellular exosome / extracellular region / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.6 Å | |||||||||
![]() | Drulyte I | |||||||||
Funding support | 1 items
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![]() | ![]() Title: Structural basis for inhibition of beta-FXIIa by garadacimab Authors: Drulyte I / Ghai R / Ow SY / Kapp EA / Quek AJ / Panousis C / Wilson MJ / Nash AD / Pelzing M | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 79 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 23.6 KB 23.6 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 9.9 KB | Display | ![]() |
Images | ![]() | 91.2 KB | ||
Masks | ![]() ![]() | 83.7 MB 83.7 MB | ![]() | |
Filedesc metadata | ![]() | 7 KB | ||
Others | ![]() ![]() ![]() ![]() | 42.4 MB 1.9 MB 77.5 MB 77.5 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8r8dMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | Sharpened map | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.095 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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-Mask #2
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-Additional map: Unsharpened map
File | emd_18999_additional_1.map | ||||||||||||
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Annotation | Unsharpened map | ||||||||||||
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-Additional map: Local resolution-filtered map
File | emd_18999_additional_2.map | ||||||||||||
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Annotation | Local resolution-filtered map | ||||||||||||
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Density Histograms |
-Half map: Half map A
File | emd_18999_half_map_1.map | ||||||||||||
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Annotation | Half map A | ||||||||||||
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Density Histograms |
-Half map: Half map B
File | emd_18999_half_map_2.map | ||||||||||||
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Annotation | Half map B | ||||||||||||
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Sample components
-Entire : Ternary complex of coagulation factor beta-XIIa with garadacimab ...
Entire | Name: Ternary complex of coagulation factor beta-XIIa with garadacimab Fab fragment and anti-LC-lambda VHH |
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Components |
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-Supramolecule #1: Ternary complex of coagulation factor beta-XIIa with garadacimab ...
Supramolecule | Name: Ternary complex of coagulation factor beta-XIIa with garadacimab Fab fragment and anti-LC-lambda VHH type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#3 |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 174 KDa |
-Macromolecule #1: Coagulation factor XII
Macromolecule | Name: Coagulation factor XII / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 26.53775 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: VVGGLVALRG AHPYIAALYW GHSFCAGSLI APCWVLTAAH CLQDRPAPED LTVVLGQERR NHSCEPCQTL AVRSYRLHEA FSPVSYQHD LALLRLQEDA DGSCALLSPY VQPVSLPSGA ARPSETTLCQ VAGWGHQFEG AEEYASFLQE AQVPFLSLER C SAPDVHGS ...String: VVGGLVALRG AHPYIAALYW GHSFCAGSLI APCWVLTAAH CLQDRPAPED LTVVLGQERR NHSCEPCQTL AVRSYRLHEA FSPVSYQHD LALLRLQEDA DGSCALLSPY VQPVSLPSGA ARPSETTLCQ VAGWGHQFEG AEEYASFLQE AQVPFLSLER C SAPDVHGS SILPGMLCAG FLEGGTDACQ GDSGGPLVCE DQAAERRLTL QGIISWGSGC GDRNKPGVYT DVAYYLAWIR EH TVSEPEA UniProtKB: Coagulation factor XII |
-Macromolecule #2: Garadacimab heavy chain variable region
Macromolecule | Name: Garadacimab heavy chain variable region / type: protein_or_peptide / ID: 2 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 25.120281 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: EVQLLESGGG LVQPGGSLRL SCAASGFTFS KYIMQWVRQA PGKGLEWVSG IDIPTKGTVY ADSVKGRFTI SRDNSKNTLY LQMNSLRAE DTAVYYCARA LPRSGYLISP HYYYYALDVW GQGTTVTVSS ASTKGPSVFP LAPCSRSTSE STAALGCLVK D YFPEPVTV ...String: EVQLLESGGG LVQPGGSLRL SCAASGFTFS KYIMQWVRQA PGKGLEWVSG IDIPTKGTVY ADSVKGRFTI SRDNSKNTLY LQMNSLRAE DTAVYYCARA LPRSGYLISP HYYYYALDVW GQGTTVTVSS ASTKGPSVFP LAPCSRSTSE STAALGCLVK D YFPEPVTV SWNSGALTSG VHTFPAVLQS SGLYSLSSVV TVPSSSLGTK TYTCNVDHKP SNTKVDKRVE SKYGPP |
-Macromolecule #3: Garadacimab light chain variable region
Macromolecule | Name: Garadacimab light chain variable region / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 22.762174 KDa |
Recombinant expression | Organism: ![]() |
Sequence | String: QSVLTQPPSA SGTPGQRVTI SCSGSSSNIG RNYVYWYQQL PGTAPKLLIY SNNQRPSGVP DRFSGSKSGT SASLAISGLR SEDEADYYC AAWDASLRGV FGGGTKLTVL GQPKAAPSVT LFPPSSEELQ ANKATLVCLI SDFYPGAVTV AWKADSSPVK A GVETTTPS ...String: QSVLTQPPSA SGTPGQRVTI SCSGSSSNIG RNYVYWYQQL PGTAPKLLIY SNNQRPSGVP DRFSGSKSGT SASLAISGLR SEDEADYYC AAWDASLRGV FGGGTKLTVL GQPKAAPSVT LFPPSSEELQ ANKATLVCLI SDFYPGAVTV AWKADSSPVK A GVETTTPS KQSNNKYAAS SYLSLTPEQW KSHRSYSCQV THEGSTVEKT VAPTECS |
-Macromolecule #5: water
Macromolecule | Name: water / type: ligand / ID: 5 / Number of copies: 18 / Formula: HOH |
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Molecular weight | Theoretical: 18.015 Da |
Chemical component information | ![]() ChemComp-HOH: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 2 mg/mL |
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Buffer | pH: 5.5 / Details: 10 mM Na Acetate, 100 mM NaCl pH 5.5 |
Grid | Model: UltrAuFoil R1.2/1.3 / Material: GOLD / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec. / Pretreatment - Atmosphere: AIR / Details: plasma current 20 mA |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 90 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV |
Details | Immediately before blotting and plunge freezing, fluorinated octyl maltoside (FOM) was added to the sample to the final concentration of 0.005%-0.01% (w/v) |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Specialist optics | Energy filter - Name: TFS Selectris / Energy filter - Slit width: 10 eV Details: Electron source E-CFEG (cold-FEG), energy filter Selectris X |
Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Number grids imaged: 2 / Number real images: 4000 / Average electron dose: 50.0 e/Å2 / Details: 2000 with 0.005% FOM and 2000 with 0.01% FOM |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.25 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 165000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |