+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-18792 | ||||||||||||
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Title | Capsid structure of Giardiavirus (GLV) CAT strain | ||||||||||||
Map data | Full map of GLV-CAT capsid | ||||||||||||
Sample |
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Keywords | Giardia parsite / dsRNA virus / capsid structure / Totiviridae / Giardia lambia virus (GLV) / VIRUS | ||||||||||||
Function / homology | Capsid protein Function and homology information | ||||||||||||
Biological species | Giardia lamblia virus | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.6 Å | ||||||||||||
Authors | Wang H / Gianluca M / Munke A / Hassan MM / Lalle M / Okamoto K | ||||||||||||
Funding support | Sweden, Norway, 3 items
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Citation | Journal: To Be Published Title: Capsid structure of Giardiavirus (GLV) CAT strain Authors: Wang H / Gianluca M / Munke A / Hassan MM / Lalle M / Okamoto K | ||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_18792.map.gz | 937.4 MB | EMDB map data format | |
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Header (meta data) | emd-18792-v30.xml emd-18792.xml | 16.1 KB 16.1 KB | Display Display | EMDB header |
Images | emd_18792.png | 266.2 KB | ||
Filedesc metadata | emd-18792.cif.gz | 5.9 KB | ||
Others | emd_18792_half_map_1.map.gz emd_18792_half_map_2.map.gz | 928.8 MB 928.8 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-18792 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-18792 | HTTPS FTP |
-Validation report
Summary document | emd_18792_validation.pdf.gz | 1.2 MB | Display | EMDB validaton report |
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Full document | emd_18792_full_validation.pdf.gz | 1.2 MB | Display | |
Data in XML | emd_18792_validation.xml.gz | 22.2 KB | Display | |
Data in CIF | emd_18792_validation.cif.gz | 26.5 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-18792 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-18792 | HTTPS FTP |
-Related structure data
Related structure data | 8r0gMC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_18792.map.gz / Format: CCP4 / Size: 1000 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||
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Annotation | Full map of GLV-CAT capsid | ||||||||||||||||||||
Voxel size | X=Y=Z: 1.06 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: Half map A of GLV-CAT capsid
File | emd_18792_half_map_1.map | ||||||||||||
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Annotation | Half map A of GLV-CAT capsid | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Half map B of GLV-CAT capsid
File | emd_18792_half_map_2.map | ||||||||||||
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Annotation | Half map B of GLV-CAT capsid | ||||||||||||
Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Giardia lamblia virus
Entire | Name: Giardia lamblia virus |
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Components |
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-Supramolecule #1: Giardia lamblia virus
Supramolecule | Name: Giardia lamblia virus / type: virus / ID: 1 / Parent: 0 / Macromolecule list: all Details: GLV isolated from G. duodenalis isolates CAT-1 from a cat NCBI-ID: 29255 / Sci species name: Giardia lamblia virus / Sci species strain: CAT-1 / Virus type: VIRION / Virus isolate: STRAIN / Virus enveloped: No / Virus empty: No |
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Host (natural) | Organism: Giardia intestinalis (eukaryote) / Strain: CAT-1 |
Virus shell | Shell ID: 1 / Name: Capsid / Diameter: 450.0 Å / T number (triangulation number): 1 |
-Macromolecule #1: Capsid protein
Macromolecule | Name: Capsid protein / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: Giardia lamblia virus |
Molecular weight | Theoretical: 103.492406 KDa |
Sequence | String: PENITLDTLN TQNDYEETHG ESPEVPKASI APAGRQNVPV LQQNQENKDN HALGGSEDAK DEREIRFSAI KTLYNYYSEG PSTPIMPHL VNRLRGLDAL AKIDATLTKV DMNAAYIFAL RPTFPYSYGY KQRFSNRRLT TSAVCYARTG LSSFLTVNKT Y TSNSPLKG ...String: PENITLDTLN TQNDYEETHG ESPEVPKASI APAGRQNVPV LQQNQENKDN HALGGSEDAK DEREIRFSAI KTLYNYYSEG PSTPIMPHL VNRLRGLDAL AKIDATLTKV DMNAAYIFAL RPTFPYSYGY KQRFSNRRLT TSAVCYARTG LSSFLTVNKT Y TSNSPLKG GSRGWPIFNV GVSPHVAEPH MRTLSPIGLE VFSQNLATFS KTLLTASSKV FTQSLYTADI LSIFGEVFLP HV MQPVSNY TPILVRALLA LIHILGPGSG NCSLSSSIFE SSIPQFFTVS HSTNMSNRTR YCLHTWSAYK DMFRNGIPPQ STL PPTLAP EGSSARVLIP AALVTSPMFP WLLALVSSGP QFFLYSKDAS INTVDIGSRG RITSPIPDVA NLDLHRLWNL FRFD GHRYI DVVIVGVDRD YVWPYQNGVY VHGGKGPNGT DNYGNADVHD GIGTIFSSFN DNVNVQTSDL ILGLLTLWNH ITTTY ATEE EVTMAIKIAA AFALVYPVQP IVYSGCPRAF QSHTSYYQPS SENCYTTDTA EVKSTWDTVE LSVQVNNAMV LGMTLP FGQ PTVSSAQWFN NIDKAEISMF KVGNLPLQNL DYLSLDMMEF YAPTTGQLYD IRSDNLILSA HRTVNLGIGY TALADFF AY LASVPAQSFY HDRMVTSPIS KQTYSVYERF IERFVDDFVG WDRCDLFNLD TLLAAKRIAG VASSPIPWHC SLQRCPLP I IMHYTGLHFG QEHITVRDVA GVEGLQQIVM RNDQGKIVVD ALGTAAPSRL AVKLDWSRLS AWYSDTTCAI PLSDRVMEI INYAAIWDPT QERRATGFVY TYFSPNFLSS FNASEPIFNT SINLTPPYDD TSHTVIQNLS MPQMLSFDPY YESTFYVVSA DNEWIPTSG PAWKVPYLEN VVKRSGRRLL AELRIASNNG SGDRTFLDDV UniProtKB: Capsid protein |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 1.0 mg/mL |
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Buffer | pH: 7.4 / Details: 1 x PBS (-) |
Grid | Model: Quantifoil R2/2 / Material: COPPER / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 20 sec. |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV |
Details | Purified GLV-CAT particles |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 30.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.7000000000000001 µm / Nominal magnification: 81000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: EMDB MAP EMDB ID: |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 2.6 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 5804 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |