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-Structure paper
| タイトル | High-resolution comparative atomic structures of two Giardiavirus prototypes infecting G. duodenalis parasite. |
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| ジャーナル・号・ページ | PLoS Pathog, Vol. 20, Issue 4, Page e1012140, Year 2024 |
| 掲載日 | 2024年4月10日 |
著者 | Han Wang / Gianluca Marucci / Anna Munke / Mohammad Maruf Hassan / Marco Lalle / Kenta Okamoto / ![]() |
| PubMed 要旨 | The Giardia lamblia virus (GLV) is a non-enveloped icosahedral dsRNA and endosymbiont virus that infects the zoonotic protozoan parasite Giardia duodenalis (syn. G. lamblia, G. intestinalis), which ...The Giardia lamblia virus (GLV) is a non-enveloped icosahedral dsRNA and endosymbiont virus that infects the zoonotic protozoan parasite Giardia duodenalis (syn. G. lamblia, G. intestinalis), which is a pathogen of mammals, including humans. Elucidating the transmission mechanism of GLV is crucial for gaining an in-depth understanding of the virulence of the virus in G. duodenalis. GLV belongs to the family Totiviridae, which infects yeast and protozoa intracellularly; however, it also transmits extracellularly, similar to the phylogenetically, distantly related toti-like viruses that infect multicellular hosts. The GLV capsid structure is extensively involved in the longstanding discussion concerning extracellular transmission in Totiviridae and toti-like viruses. Hence, this study constructed the first high-resolution comparative atomic models of two GLV strains, namely GLV-HP and GLV-CAT, which showed different intracellular localization and virulence phenotypes, using cryogenic electron microscopy single-particle analysis. The atomic models of the GLV capsids presented swapped C-terminal extensions, extra surface loops, and a lack of cap-snatching pockets, similar to those of toti-like viruses. However, their open pores and absence of the extra crown protein resemble those of other yeast and protozoan Totiviridae viruses, demonstrating the essential structures for extracellular cell-to-cell transmission. The structural comparison between GLV-HP and GLV-CAT indicates the first evidence of critical structural motifs for the transmission and virulence of GLV in G. duodenalis. |
リンク | PLoS Pathog / PubMed:38598600 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 2.14 - 2.6 Å |
| 構造データ | EMDB-18791, PDB-8r0f: EMDB-18792, PDB-8r0g: |
| 由来 |
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キーワード | VIRUS / Giardia parasite / dsRNA virus / capsid structure / cryo / Totiviridae / Giardia lambia virus (GLV) / Giardia parsite |
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giardia lamblia virus (ウイルス)
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