Entry Database : EMDB / ID : EMD-18529 Downloads & linksTitle Cryo-EM structure of a human spliceosomal B complex protomer Map data Details SampleComplex : human spliceosomal B complexProtein or peptide : x 48 typesRNA : x 5 types Details Keywords spliceosome / SPLICINGFunction / homology Function and homology informationFunction Domain/homology Component
DNA topoisomerase binding / microfibril / Lsm2-8 complex / U6 snRNA 3'-end binding / spliceosomal snRNP complex / ribonucleoprotein complex localization / snRNP binding / U4atac snRNP / positive regulation of cytotoxic T cell differentiation / maturation of 5S rRNA ... DNA topoisomerase binding / microfibril / Lsm2-8 complex / U6 snRNA 3'-end binding / spliceosomal snRNP complex / ribonucleoprotein complex localization / snRNP binding / U4atac snRNP / positive regulation of cytotoxic T cell differentiation / maturation of 5S rRNA / RNA localization / protein kinase B binding / U4atac snRNA binding / mRNA decay by 5' to 3' exoribonuclease / Lsm1-7-Pat1 complex / U11/U12 snRNP / U6 snRNP / box C/D sno(s)RNA binding / positive regulation of primary miRNA processing / PH domain binding / U2 snRNP binding / U7 snRNA binding / histone pre-mRNA DCP binding / U7 snRNP / alternative mRNA splicing, via spliceosome / dense fibrillar component / B-WICH complex / histone pre-mRNA 3'end processing complex / cis assembly of pre-catalytic spliceosome / SLBP independent Processing of Histone Pre-mRNAs / SLBP Dependent Processing of Replication-Dependent Histone Pre-mRNAs / mRNA splice site recognition / splicing factor binding / spliceosome conformational change to release U4 (or U4atac) and U1 (or U11) / transcription elongation factor activity / protein methylation / U12-type spliceosomal complex / methylosome / P-body assembly / U4/U6 snRNP / 7-methylguanosine cap hypermethylation / positive regulation of androgen receptor activity / U1 snRNP binding / mRNA 3'-end processing / blastocyst formation / pICln-Sm protein complex / U2-type catalytic step 1 spliceosome / protein localization to kinetochore / RNA splicing, via transesterification reactions / small nuclear ribonucleoprotein complex / sno(s)RNA-containing ribonucleoprotein complex / SMN-Sm protein complex / spliceosomal tri-snRNP complex / P granule / U4 snRNA binding / telomerase holoenzyme complex / box C/D methylation guide snoRNP complex / U2-type spliceosomal complex / mRNA cis splicing, via spliceosome / U2-type precatalytic spliceosome / commitment complex / Transport of Mature mRNA derived from an Intron-Containing Transcript / telomerase RNA binding / proline-rich region binding / U2-type prespliceosome assembly / U2-type catalytic step 2 spliceosome / ubiquitin-like protein conjugating enzyme binding / U4 snRNP / RNA polymerase binding / rRNA modification in the nucleus and cytosol / box C/D snoRNP assembly / SAGA complex / U2 snRNP / positive regulation of mRNA splicing, via spliceosome / RNA Polymerase II Transcription Termination / positive regulation of transcription by RNA polymerase III / tRNA processing / U1 snRNP / positive regulation of protein targeting to mitochondrion / U3 snoRNA binding / nuclear-transcribed mRNA catabolic process / U2-type prespliceosome / K63-linked polyubiquitin modification-dependent protein binding / cyclosporin A binding / positive regulation of miRNA metabolic process / positive regulation of transcription by RNA polymerase I / precatalytic spliceosome / mitotic spindle assembly checkpoint signaling / spliceosomal complex assembly / oligodendrocyte differentiation / mRNA Splicing - Minor Pathway / negative regulation of transcription elongation by RNA polymerase II / regulation of alternative mRNA splicing, via spliceosome / mRNA catabolic process / mRNA 5'-splice site recognition / regulation of RNA splicing / mRNA 3'-splice site recognition / Processing of Capped Intron-Containing Pre-mRNA / MLL1 complex / spliceosomal tri-snRNP complex assembly Similarity search - Function Protein RED, C-terminal / RED-like, N-terminal / Protein Red-like / RED-like protein C-terminal region / RED-like protein N-terminal region / : / SRSF1, RNA recognition motif 1 / WD40 repeat-containing protein SMU1 / WW domain-binding protein 4 / Ubiquitin-like modifier Hub1/Ubl5 ... Protein RED, C-terminal / RED-like, N-terminal / Protein Red-like / RED-like protein C-terminal region / RED-like protein N-terminal region / : / SRSF1, RNA recognition motif 1 / WD40 repeat-containing protein SMU1 / WW domain-binding protein 4 / Ubiquitin-like modifier Hub1/Ubl5 / LisH-like dimerisation domain / Pre-mRNA-splicing factor 38, C-terminal / Pre-mRNA-splicing factor 38-associated hydrophilic C-term / Micro-fibrillar-associated protein 1, C-terminal / Microfibrillar-associated protein 1 / Microfibril-associated/Pre-mRNA processing / U4/U6.U5 small nuclear ribonucleoprotein component Snu23 / Pre-mRNA-splicing factor 38 / Transcription elongation regulator 1-like / PRP38 family / U1-C, C2H2-type zinc finger / U1 zinc finger / PRP4-like superfamily / pre-mRNA processing factor 4 (PRP4) like / C-terminal to LisH motif. / CTLH, C-terminal LisH motif / C-terminal to LisH (CTLH) motif profile. / SF3B6, RNA recognition motif / FF domain / SF3B4, RNA recognition motif 2 / SNU66/SART1 family / HIND motif / SART-1 family / HIND motif / Splicing factor SF3a60 binding domain / Splicing factor SF3a60 binding domain / FF domain / PWI domain superfamily / FF domain superfamily / PWI domain / PWI domain profile. / FF domain profile. / Contains two conserved F residues / Sm-like protein Lsm8 / : / Replication stress response SDE2 C-terminal / Cactus-binding C-terminus of cactin protein / U6 snRNA-associated Sm-like protein Lsm1/8 / Pre-mRNA processing factor 4 (PRP4)-like / U6 snRNA-associated Sm-like protein LSm2 / Sm-like protein Lsm4 / Splicing factor 3A subunit 1, ubiquitin domain / Splicing Factor Motif, present in Prp18 and Pr04 / Sm-like protein Lsm7 / Sm-like protein LSm5 / Sm-like protein Lsm3 / U2 small nuclear ribonucleoprotein B'', RNA recognition motif 2 / U2 small nuclear ribonucleoprotein B'', RNA recognition motif 1 / U6 snRNA-associated Sm-like protein Lsm3 / Pre-mRNA-splicing factor 3 / U4/U6 small nuclear ribonucleoprotein Prp3 / pre-mRNA processing factor 3 domain / Prp31 C-terminal / U4/U6 small nuclear ribonucleoprotein Prp31 / Prp31 C terminal domain / Small nuclear ribonucleoprotein Prp3, C-terminal domain / SF3A2 domain / Small nuclear ribonucleoprotein Prp3, C-terminal domain / Pre-mRNA-splicing factor SF3a complex subunit 2 (Prp11) / PWI domain / Splicing factor SF3a60 /Prp9 subunit, C-terminal / SF3A3 domain / SF3B4, RNA recognition motif 1 / SF3a60/Prp9 C-terminal / Pre-mRNA-splicing factor SF3A3, of SF3a complex, Prp9 / PWI, domain in splicing factors / BUD31/G10-related, conserved site / G10 protein signature 1. / G10 protein signature 2. / Dim1 family / Mitosis protein DIM1 / Mitosis protein DIM1 / Splicing factor 3B, subunit 5 / PRP1 splicing factor, N-terminal / PRP1 splicing factor, N-terminal / Splicing factor 3A subunit 1 / Splicing factor 3B subunit 1 / Splicing factor 3B subunit 1 / Splicing factor 3A subunit 1, conserved domain / Pre-mRNA splicing factor PRP21 like protein / G10 protein / Domain of unknown function DUF382 / Pre-mRNA-splicing factor BUD31 / Domain of unknown function (DUF382) / SWAP/Surp / SWAP/Surp superfamily / Surp module / Zinc-finger of C2H2 type / SURP motif repeat profile. / Suppressor-of-White-APricot splicing regulator Similarity search - Domain/homology Transcription elongation regulator 1 / U4/U6 small nuclear ribonucleoprotein Prp4 / U4/U6.U5 tri-snRNP-associated protein 1 / U4/U6 small nuclear ribonucleoprotein Prp3 / Peptidyl-prolyl cis-trans isomerase H / Splicing factor 3B subunit 1 / WW domain-binding protein 4 / U5 small nuclear ribonucleoprotein 200 kDa helicase / Pre-mRNA-processing factor 6 / U6 snRNA-associated Sm-like protein LSm8 ... Transcription elongation regulator 1 / U4/U6 small nuclear ribonucleoprotein Prp4 / U4/U6.U5 tri-snRNP-associated protein 1 / U4/U6 small nuclear ribonucleoprotein Prp3 / Peptidyl-prolyl cis-trans isomerase H / Splicing factor 3B subunit 1 / WW domain-binding protein 4 / U5 small nuclear ribonucleoprotein 200 kDa helicase / Pre-mRNA-processing factor 6 / U6 snRNA-associated Sm-like protein LSm8 / U2 small nuclear ribonucleoprotein B'' / U2 small nuclear ribonucleoprotein A' / Small nuclear ribonucleoprotein-associated proteins B and B' / Protein BUD31 homolog / Microfibrillar-associated protein 1 / NHP2-like protein 1 / Small nuclear ribonucleoprotein E / Small nuclear ribonucleoprotein F / Small nuclear ribonucleoprotein G / U6 snRNA-associated Sm-like protein LSm3 / U6 snRNA-associated Sm-like protein LSm6 / Small nuclear ribonucleoprotein Sm D1 / Small nuclear ribonucleoprotein Sm D2 / Small nuclear ribonucleoprotein Sm D3 / Thioredoxin-like protein 4A / Serine/arginine-rich splicing factor 1 / Splicing factor 3A subunit 3 / Protein Red / Splicing factor 3B subunit 2 / 116 kDa U5 small nuclear ribonucleoprotein component / Splicing factor 3B subunit 3 / Splicing factor 3B subunit 4 / Splicing factor 3A subunit 2 / Splicing factor 3A subunit 1 / WD40 repeat-containing protein SMU1 / Pre-mRNA-processing-splicing factor 8 / PHD finger-like domain-containing protein 5A / Pre-mRNA-splicing factor 38A / U4/U6 small nuclear ribonucleoprotein Prp31 / U5 small nuclear ribonucleoprotein 40 kDa protein / Zinc finger matrin-type protein 2 / Splicing factor 3B subunit 5 / Ubiquitin-like protein 5 / U6 snRNA-associated Sm-like protein LSm7 / U6 snRNA-associated Sm-like protein LSm2 / Splicing factor 3B subunit 6 / U6 snRNA-associated Sm-like protein LSm5 / U6 snRNA-associated Sm-like protein LSm4 Similarity search - ComponentBiological species Homo sapiens (human)Method single particle reconstruction / cryo EM / Resolution : 5.29 Å Details AuthorsZhang Z / Kumar V / Dybkov O / Will CL / Urlaub H / Stark H / Luehrmann R Funding support Germany, 1 items Details Hide detailsOrganization Grant number Country German Research Foundation (DFG) Germany
CitationJournal : To Be Published Title : New insights into the functions of B complex proteins revealed by cryo-EM of dimerized spliceosomesAuthors : Zhang Z / Kumar V / Dybkov O / Will CL / Urlaub H / Stark H / Luehrmann R History Deposition Sep 28, 2023 - Header (metadata) release Jan 24, 2024 - Map release Jan 24, 2024 - Update Jan 24, 2024 - Current status Jan 24, 2024 Processing site : PDBe / Status : Released
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