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データを開く
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基本情報
登録情報 | ![]() | ||||||||||||||||||
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タイトル | X. laevis CMG dimer bound to dimeric DONSON - MCM ATPase | ||||||||||||||||||
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![]() | DNA replication initiation / Xenopus egg extract / primordial dwarfism / replicative helicase / genome stability / REPLICATION | ||||||||||||||||||
機能・相同性 | ![]() nuclear DNA replication / premeiotic DNA replication / mitotic DNA replication / CMG complex / single-stranded 3'-5' DNA helicase activity / MCM complex / mitotic DNA replication checkpoint signaling / mitotic DNA replication initiation / double-strand break repair via break-induced replication / replisome ...nuclear DNA replication / premeiotic DNA replication / mitotic DNA replication / CMG complex / single-stranded 3'-5' DNA helicase activity / MCM complex / mitotic DNA replication checkpoint signaling / mitotic DNA replication initiation / double-strand break repair via break-induced replication / replisome / regulation of DNA-templated DNA replication initiation / single-stranded DNA helicase activity / DNA strand elongation involved in DNA replication / replication fork processing / DNA unwinding involved in DNA replication / mitotic G2 DNA damage checkpoint signaling / 3'-5' DNA helicase activity / DNA replication origin binding / DNA replication initiation / replication fork / DNA damage checkpoint signaling / single-stranded DNA binding / DNA helicase / DNA replication / cell cycle / cell division / chromatin binding / chromatin / ATP hydrolysis activity / ATP binding / nucleus / metal ion binding 類似検索 - 分子機能 | ||||||||||||||||||
生物種 | ![]() | ||||||||||||||||||
手法 | 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.53 Å | ||||||||||||||||||
![]() | Cvetkovic MA / Costa A | ||||||||||||||||||
資金援助 | ![]()
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![]() | ![]() タイトル: The structural mechanism of dimeric DONSON in replicative helicase activation. 著者: Milos A Cvetkovic / Paolo Passaretti / Agata Butryn / Alicja Reynolds-Winczura / Georgia Kingsley / Aggeliki Skagia / Cyntia Fernandez-Cuesta / Divyasree Poovathumkadavil / Roger George / ...著者: Milos A Cvetkovic / Paolo Passaretti / Agata Butryn / Alicja Reynolds-Winczura / Georgia Kingsley / Aggeliki Skagia / Cyntia Fernandez-Cuesta / Divyasree Poovathumkadavil / Roger George / Anoop S Chauhan / Satpal S Jhujh / Grant S Stewart / Agnieszka Gambus / Alessandro Costa / ![]() 要旨: The MCM motor of the replicative helicase is loaded onto origin DNA as an inactive double hexamer before replication initiation. Recruitment of activators GINS and Cdc45 upon S-phase transition ...The MCM motor of the replicative helicase is loaded onto origin DNA as an inactive double hexamer before replication initiation. Recruitment of activators GINS and Cdc45 upon S-phase transition promotes the assembly of two active CMG helicases. Although work with yeast established the mechanism for origin activation, how CMG is formed in higher eukaryotes is poorly understood. Metazoan Downstream neighbor of Son (DONSON) has recently been shown to deliver GINS to MCM during CMG assembly. What impact this has on the MCM double hexamer is unknown. Here, we used cryoelectron microscopy (cryo-EM) on proteins isolated from replicating Xenopus egg extracts to identify a double CMG complex bridged by a DONSON dimer. We find that tethering elements mediating complex formation are essential for replication. DONSON reconfigures the MCM motors in the double CMG, and primordial dwarfism patients' mutations disrupting DONSON dimerization affect GINS and MCM engagement in human cells and DNA synthesis in Xenopus egg extracts. | ||||||||||||||||||
履歴 |
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構造の表示
添付画像 |
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-EMDBアーカイブ
マップデータ | ![]() | 567.7 MB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 25.4 KB 25.4 KB | 表示 表示 | ![]() |
FSC (解像度算出) | ![]() | 17.9 KB | 表示 | ![]() |
画像 | ![]() | 112 KB | ||
Filedesc metadata | ![]() | 9.1 KB | ||
その他 | ![]() ![]() | 557.5 MB 557.5 MB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-検証レポート
文書・要旨 | ![]() | 1.2 MB | 表示 | ![]() |
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文書・詳細版 | ![]() | 1.2 MB | 表示 | |
XML形式データ | ![]() | 27 KB | 表示 | |
CIF形式データ | ![]() | 35.3 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
EMDBのページ | ![]() ![]() |
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「今月の分子」の関連する項目 |
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マップ
ファイル | ![]() | ||||||||||||||||||||
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ボクセルのサイズ | X=Y=Z: 0.95 Å | ||||||||||||||||||||
密度 |
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対称性 | 空間群: 1 | ||||||||||||||||||||
詳細 | EMDB XML:
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-添付データ
-ハーフマップ: #2
ファイル | emd_18192_half_map_1.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
-ハーフマップ: #1
ファイル | emd_18192_half_map_2.map | ||||||||||||
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投影像・断面図 |
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密度ヒストグラム |
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試料の構成要素
-全体 : X. laevis CMG dimer bound to dimeric DONSON
全体 | 名称: X. laevis CMG dimer bound to dimeric DONSON |
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要素 |
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-超分子 #1: X. laevis CMG dimer bound to dimeric DONSON
超分子 | 名称: X. laevis CMG dimer bound to dimeric DONSON / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1-#7 |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 1.54 MDa |
-分子 #1: DNA replication licensing factor mcm2
分子 | 名称: DNA replication licensing factor mcm2 / タイプ: protein_or_peptide / ID: 1 / コピー数: 1 / 光学異性体: LEVO / EC番号: DNA helicase |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 100.397648 KDa |
配列 | 文字列: MADSSESFNI ATSPRAGSRR DALTSSPGRD LPPFEDESEG MFGDGVVPEE EEDGEELIGD AMERDYRPIS ELDRYEVEGL DDEEDVEDL TASQREAAEQ SMRMRDREMG RELGRMRRGL LYDSDEEEED RPARKRRMAE RAAEGAPEED EEMIESIENL E DMKGHTVR ...文字列: MADSSESFNI ATSPRAGSRR DALTSSPGRD LPPFEDESEG MFGDGVVPEE EEDGEELIGD AMERDYRPIS ELDRYEVEGL DDEEDVEDL TASQREAAEQ SMRMRDREMG RELGRMRRGL LYDSDEEEED RPARKRRMAE RAAEGAPEED EEMIESIENL E DMKGHTVR EWVSMAATRL EIYHRFKNFL RTHVDEHGHN VFKEKISDMC KENKESLPVN YEDLAAREHV LAYFLPEAPA EM LKIFDEA AKEVVLVMYP KYDRIAREIH VRISHLPLVE ELRSLRQLHL NQLIRTSGVV TCCTGVLPQL SMVKYNCNKC NFI LGPFFQ SQNQEVRPGS CPECQSFGPF EINMEETVYQ NYQRITIQES PGKVAAGRLP RSKDAILLAD LVDSCKPGDE IELT GIYHN NYDGSLNTAN GFPVFATVIL ANHITKKDDK VAVGELTDED VKAIVALSKD ERIGERIFAS IAPSIYGHED IKRGL ALAL FGGEAKNPGG KHKVRGDINV LLCGDPGTAK SQFLKYVEKV ASRAVFTTGQ GASAVGLTAY VQRHPVTKEW TLEAGA LVL ADRGVCLIDE FDKMNDQDRT SIHEAMEQQS ISISKAGIVT SLQARCTVIA ASNPIGGRYD PSLTFSENVD LTEPIVS RF DILCVVRDTV DPVQDEMLAR FVVSSHIKHH PSSKDIANGD AAEFALPNTF GVEALPQEVL KKYIMYAKEK IRPKLNQM D QDKVAKMYSD LRKESMATGS IPITVRHIES MIRMAEAHAR MHLRDYVVED DVNMAIRVML ESFIDTQKFS VMRSMRKTF ARYLAFRRDN NELLLFVLKQ LIAEQVTYQR NRYGAQQDTI EVPEKDLVDK ARQINIHNLS AFYDSDLFKM NKFTHDVKKK LIIQQF UniProtKB: DNA replication licensing factor mcm2 |
-分子 #2: Maternal DNA replication licensing factor mcm3
分子 | 名称: Maternal DNA replication licensing factor mcm3 / タイプ: protein_or_peptide / ID: 2 / コピー数: 1 / 光学異性体: LEVO / EC番号: DNA helicase |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 90.5365 KDa |
配列 | 文字列: MDYGGGFEDH ELREAQREYL DFLDDDQDQG LYHGKVRDMI GSNEHRLIVN LNDVRRKNDK RANLMLNDAF AETIAFQRAL KDLVASIDA TYAKQFEEFS VGFEGSFGSK HVSPRTLTAS LLGSLVCVEG IVTKCSLVRP KVMRSVHYCP ATKKTLERKY S DLTSLEAF ...文字列: MDYGGGFEDH ELREAQREYL DFLDDDQDQG LYHGKVRDMI GSNEHRLIVN LNDVRRKNDK RANLMLNDAF AETIAFQRAL KDLVASIDA TYAKQFEEFS VGFEGSFGSK HVSPRTLTAS LLGSLVCVEG IVTKCSLVRP KVMRSVHYCP ATKKTLERKY S DLTSLEAF PSSSIYPTKD EENNPLETEY GLSTYKDHQT LSIQEMPEKA PAGQLPRSVD IIADDDLVDK CKPGDRVQIV GI YRCLPSK QGGFTSGTFR TILLANNIKL MSKEIAPTFS ADDVAKIKKF CKAHSKDIFE HLSKSLAPSI HGHEYIKKAI LCM LLGGNE KVLENGTRIR GDINVLLIGD PSVAKSQLLR YVLHTAPRAI PTTGRGSSGV GLTAAVTTDQ ETGERRLEAG AMVL ADRGV VCIDEFDKMS DMDRTAIHEV MEQGRVTIAK AGIQARLNAR CSVLAAANPV YGRYDQYRTP MENIGLQDSL LSRFD LLFI VLDKMDADND QEIADHVLRM HRYRTPGEQD GYALPLGCSV EIFATDDPNA SDVTDQELQI YEKHDNLLHG PRKNKS KIV SMQFIRKYIH VAKLIKPVLT SEAADYISQE YAKIRNHDQI NNDSARTMPV TARALETMIR LSTAHAKVRM SKTIERQ DA ETALELVQFA YFKKVLAKEK KKTDKDLHDE NLSQDTLSQE SVRKSSRRAG KIADSQDDSM DPYSFSEQDS SLNENLSQ S LRPQRKKAES QDGKRSLSQN RTKEFKAALL KAFKSSRSQS VAVSQLLELI NKGNPEPFER SEVKEALDNM QNDNQVMVS EDVVFLI UniProtKB: Maternal DNA replication licensing factor mcm3 |
-分子 #3: DNA replication licensing factor mcm4-B
分子 | 名称: DNA replication licensing factor mcm4-B / タイプ: protein_or_peptide / ID: 3 / コピー数: 1 / 光学異性体: LEVO / EC番号: DNA helicase |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 97.256305 KDa |
配列 | 文字列: MSSPTSTPSR RRNKRGRGSN PPTPHGEEVQ SPPSQRRRTE DSTSIGELLP MPTSPSGDVQ SPSGQELLFS SPAPSRHSAH QSELDLSSP LTYGTPSSRV EGTPRSGIRG TPARQRPDLG SARKVKQVDL HSDQPAAEEL VTSEQSLGQK LVIWGTDVNV A TCKEKFQR ...文字列: MSSPTSTPSR RRNKRGRGSN PPTPHGEEVQ SPPSQRRRTE DSTSIGELLP MPTSPSGDVQ SPSGQELLFS SPAPSRHSAH QSELDLSSP LTYGTPSSRV EGTPRSGIRG TPARQRPDLG SARKVKQVDL HSDQPAAEEL VTSEQSLGQK LVIWGTDVNV A TCKEKFQR FVQRFIDPSA KEEDNVGLDL NEPIYMQRLE EINVVGDPFL NIDCDHLRNF DQDLYRQLVC YPQEVIPTFD MA ANEIFFE RYPDSILEHQ IQVRPYNALK TRNMRSLNPE DIDQLITISG MVIRTSQIIP EMQEAFFKCQ VCAFTTRVEI DRG RIAEPS VCKHCNTTHS MALIHNRSMF SDKQMIKLQE SPEDMPAGQT PHTTILYGHN DLVDKVQPGD RVNVTGIYRA VPIR VNPRV RNVKSVYKTH IDVIHYRKTD SKRLHGIDED TEQKLFTEER VAMLKELAAK PDIYERLAAA LAPSIYEHED IKKGI LLQL FGGTRKDFSH TGRGKFRAEV NILLCGDPGT SKSQLLQYVF NLVPRGQYTS GKGSSAVGLT AYVMKDPETR QLVLQT GAL VLSDNGICCI DEFDKMNEST RSVLHEVMEQ QTLSIAKAGI ICQLNARTSV LAAANPVESQ WNPKKTTIEN IQLPHTL LS RFDLIFLMLD PQDEAYDRRL AHHLVALYYQ SEEQMKEEHL DMAVLKDYIA YARTYVNPRL SEEASQALIE AYVSMRKI G SGRGMVSAYP RQLESLIRLS EAHAKVRFSN KVETIDVEEA KRLHREALKQ SATDPRTGIV DISILTTGMS ATARKRKEE LAQVLKKLIQ SKGKTPALKY QQLFEDLRGQ SDAAITKDMF DEALHALADD DYLTVTGKTV RLL UniProtKB: DNA replication licensing factor mcm4-B |
-分子 #4: DNA replication licensing factor mcm5-A
分子 | 名称: DNA replication licensing factor mcm5-A / タイプ: protein_or_peptide / ID: 4 / コピー数: 1 / 光学異性体: LEVO / EC番号: DNA helicase |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 82.556414 KDa |
配列 | 文字列: MSGFDDLGVY YSDSFGGEQQ VGDDGQAKKS QLKKRFREFL RQYRIGTDRT GFTFKYRDEL KRHYNLGEYW IEVEMEDLAS FDEDLADYL YKQPTEHLQL LEEAAQEVAD EVTRPRPAGE ETIQEIQVML RSDANPANIR SLKSEQMSHL VKIPGIIIAA T AVRAKATK ...文字列: MSGFDDLGVY YSDSFGGEQQ VGDDGQAKKS QLKKRFREFL RQYRIGTDRT GFTFKYRDEL KRHYNLGEYW IEVEMEDLAS FDEDLADYL YKQPTEHLQL LEEAAQEVAD EVTRPRPAGE ETIQEIQVML RSDANPANIR SLKSEQMSHL VKIPGIIIAA T AVRAKATK ISIQCRSCRN TIGNIAVRPG LEGYAMPRKC NTEQAGRPNC PLDPYFIIPD KCKCVDFQTL KLQESPDAVP HG ELPRHMQ LYCDRYLCDK VVPGNRVTIM GIYSIRKSGK TSTKGRDRVG VGIRSSYIRV VGIQVDTEGT GRSAAGAITP QEE EEFRRL AAKPDIYETV AKSIAPSIYG SSDIKKAIAC LLFGGSRKRL PDGLTRRGDV NLLMLGDPGT AKSQLLKFVE RCSP IGVYT SGKGSSAAGL TASVMRDPVS RNFIMEGGAM VLADGGVVCI DEFDKMREDD RVAIHEAMEQ QTISIAKAGI TTTLN SRCS VLAAANSVYG RWDDTKGEEN IDFMPTILSR FDMIFIVKDE HNEQRDMTLA KHVMNVHLSA RTQSSSVEGE VDLNTL KKY IAYCRAKCGP RLSAEAAEKL KNRYILMRSG AREHERETEK RSSIPITVRQ LEAIVRISES LGKMKLQPFA TETDVEE AL RLFQVSTLDA AMSGSLSGVE GFTTQEDQEM LSRIEKQMKK RFAIGSQVSE HSIIQDFLKQ KYPEHAIHKV LSLMMRRG E IQHRLQRKVL YRIK UniProtKB: DNA replication licensing factor mcm5-A |
-分子 #5: Maternal DNA replication licensing factor mcm6
分子 | 名称: Maternal DNA replication licensing factor mcm6 / タイプ: protein_or_peptide / ID: 5 / コピー数: 1 / 光学異性体: LEVO / EC番号: DNA helicase |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 92.752969 KDa |
配列 | 文字列: MELGGPAAAG DTDIAGQQLF KDELSDKCQK LFLEFLEECK GKDGSNLYVS AAEELIRPER NTLAVNFTDI EYYNQQLATT IQEEYYRVY PHLCRAVRSF ARQMGNIPAN KEFYIAFSDF PARQKIRELS SAKIGTLLRI SGQVVRTHPV HPELVSGTFL C MDCQSIVK ...文字列: MELGGPAAAG DTDIAGQQLF KDELSDKCQK LFLEFLEECK GKDGSNLYVS AAEELIRPER NTLAVNFTDI EYYNQQLATT IQEEYYRVY PHLCRAVRSF ARQMGNIPAN KEFYIAFSDF PARQKIRELS SAKIGTLLRI SGQVVRTHPV HPELVSGTFL C MDCQSIVK DVEQQFRYTQ PTICKNPVCA NRRRFTLDTN KSRFVDFQKV RIQETQAELP RGAIPRSVEI ILRAEAVESA MA GDRCDFT GTLIVVPDVS ALAAGDARME TGAKVTGGEG FNSEGVQGLK ALGVRDLSYR LAFLACYVGA TNPRFGGKDL REE DQTAES IKNQMTVQEW EKVFEMSQDK NLYHNLCTSL FPTIHGNDEI KRGVLLMLFG GVPKTTMEGT SLRGDINVCI VGDP STSKS QFLKHVEEFS PRAVYTSGKA SSAAGLTAAV VKDEESHEFV IEAGALMLAD NGVCCIDEFD KMDLKDQVAI HEAME QQTI SITKAGVKAT LNARTSILAA ANPVGGRYER SKSLKHNVNL SAPIMSRFDL FFILVDECNE VTDYAIARRI VDLHAR NEE SIERVYSIED IQRYLLFARQ FQPKITKEAE EFIVEQYRRL RQRDGSGVAK SSWRITVRQL ESLIRLSESM ARMHCSD EV QPKHVKEAFR LLSKSIIRVD TPDVSFDQGE DEKNIEGENN GNLNNGEEAM ETNQDEPINE KPSSNAGLKM SFAEYKQI S NLLVLYMQKM EETEEECHLT TTDLVNWYLK EMEAEIETET ELILKKRLIE KVIHRLIYYD HILIELNKSE LKTMDDTKE TGEDAAEDRI LVVNPNYMLE D UniProtKB: Maternal DNA replication licensing factor mcm6 |
-分子 #6: DNA replication licensing factor mcm7-B
分子 | 名称: DNA replication licensing factor mcm7-B / タイプ: protein_or_peptide / ID: 6 / コピー数: 1 / 光学異性体: LEVO / EC番号: DNA helicase |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 81.841539 KDa |
配列 | 文字列: MPRDYQAEKE KCKTFLQEFY KDDEFGKKNF KYGVQLANIA HREQVALCID LDDLAEEDPE LVDAICENTR RYTNLFADAV QELLPQYKE REVVHKDALD VYIEHRLMME QRGRDPNEMR DPHNQYPPEL MRRFELYFKA PSSSKARVVR DVKADSIGKL V TVRGIVTR ...文字列: MPRDYQAEKE KCKTFLQEFY KDDEFGKKNF KYGVQLANIA HREQVALCID LDDLAEEDPE LVDAICENTR RYTNLFADAV QELLPQYKE REVVHKDALD VYIEHRLMME QRGRDPNEMR DPHNQYPPEL MRRFELYFKA PSSSKARVVR DVKADSIGKL V TVRGIVTR VTEVKPMMVV ATYTCDQCGA ETYQPIQSPT FMPLIMCPSR ECQTNRSGGR LYLQTRGSKF IKFQELKIQE HS DQVPVGN IPRCMSVYVR GENTRLAQPG DHVGITGVFL PMLRTGFRQV VQGLLSETYL ESHRLVKMNK TEDDELGTEE LSE EELRQI TEEDFYEKLA ASIAPEIYGH EDVKKALLLL LVGGVDHSPR GMKIRGNINV CLMGDPGVAK SQLLSYIDRL APRS QYTTG RGSSGVGLTA AVMKDPVTGE MTLEGGALVL ADQGVCCIDE FDKMMDSDRT AIHEVMEQQT ISIAKAGIMT TLNAR CSIL AAANPAYGRY NPKKTVEQNI QLPAALLSRF DLLWLIQDKP DRDNDLRLAQ HITYVHQHSK QPPSQFQPMD MKLMRR YIT MCKSKQPAIP ESLADYLTAA YVEMRKEART NKDMTFTSAR TLLSILRLST ALARLRLEDV VEKEDVNEAM RLTEMSK DS LQGDKGHASR TQRPADVIFS TIREMVPEKG ARSVKYSEAE QRCVSKGFTP AQFEAALEEY EELNVWLVNQ ARTKITFV UniProtKB: DNA replication licensing factor mcm7-B |
-分子 #7: Protein downstream neighbor of son homolog
分子 | 名称: Protein downstream neighbor of son homolog / タイプ: protein_or_peptide / ID: 7 / コピー数: 1 / 光学異性体: LEVO |
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由来(天然) | 生物種: ![]() |
分子量 | 理論値: 63.864559 KDa |
配列 | 文字列: MAELLPGYSP SFKKPSEILR LSRRRSRSEA SKTGLSPFSP GDVIKRVPGL RPFSPGPNKG AGVKRRNPFA SLENTVCSPV KRRAETVAE YGAASLEPRA LSAVRPSCLG QDSPEPPQFN SVEDVIWGDP LAADADPLVK TESPEKPAPA CEIPKGSVTF P ADWSLKTR ...文字列: MAELLPGYSP SFKKPSEILR LSRRRSRSEA SKTGLSPFSP GDVIKRVPGL RPFSPGPNKG AGVKRRNPFA SLENTVCSPV KRRAETVAE YGAASLEPRA LSAVRPSCLG QDSPEPPQFN SVEDVIWGDP LAADADPLVK TESPEKPAPA CEIPKGSVTF P ADWSLKTR LLFTSSHSFS WADHLKAQEE AQGLVMQCRA TAVNLPHSIQ EPKLSTDLRC AFQQSLVHWI HPSLPWVQLF PR IGVDRKM AGKNTPWSQD ESLQQVLMSE WALSFTSLYN LLKAKLCPYF YVCTYQFTVL FRAAGLAGSD VITAVMSPTT RGL REAMKN EGITFSQPLV EDDTGKKQKK PEAASQGDIN PEKENGTAEA DEASDESDED ESFSWLEEMG VEDKIKKPDS ISIK LRKEK NEVKLDHKPE SVVLVKGTNT FTLLNFLINC KSIVAAAGLQ AGLPPTLLSP VAFRGATMHA LKARSVNVKT RVNSG YKDQ FSLEITGPIM PHSLHSLTML LQSAQRGSFS AGLYTHEPTA VFNTPIHSQA VKEISADLQN CGLHPCTVEQ LTQVNE LGK LSLRHLEMTD YRYTWK UniProtKB: Protein downstream neighbor of son homolog |
-分子 #8: ADENOSINE-5'-TRIPHOSPHATE
分子 | 名称: ADENOSINE-5'-TRIPHOSPHATE / タイプ: ligand / ID: 8 / コピー数: 5 / 式: ATP |
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分子量 | 理論値: 507.181 Da |
Chemical component information | ![]() ChemComp-ATP: |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | 単粒子再構成法 |
試料の集合状態 | particle |
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試料調製
緩衝液 | pH: 7.5 |
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凍結 | 凍結剤: ETHANE / 装置: FEI VITROBOT MARK IV |
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電子顕微鏡法
顕微鏡 | FEI TITAN KRIOS |
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撮影 | フィルム・検出器のモデル: FEI FALCON IV (4k x 4k) 平均電子線量: 33.0 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 3.4 µm / 最小 デフォーカス(公称値): 1.3 µm |
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |