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- EMDB-17337: CryoEM structure of 20S Trichomonas vaginalis proteasome in compl... -
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Open data
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Basic information
Entry | ![]() | |||||||||
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Title | CryoEM structure of 20S Trichomonas vaginalis proteasome in complex with proteasome inhibitor CP-17 | |||||||||
![]() | CryoEM map of 20S Trichomonas vaginalis proteasome in complex with proteasome inhibitor CP-17 | |||||||||
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![]() | Proteasome / 20S / Trichomonas vaginalis / covalently bound protease inhibitor CP-17 / HYDROLASE | |||||||||
Function / homology | ![]() proteasome endopeptidase complex / proteasome core complex, beta-subunit complex / proteasome core complex, alpha-subunit complex / threonine-type endopeptidase activity / proteasome-mediated ubiquitin-dependent protein catabolic process / endopeptidase activity / nucleus / cytoplasm / cytosol Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.65 Å | |||||||||
![]() | Silhan J / Boura E / Fajtova P | |||||||||
Funding support | European Union, 1 items
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![]() | ![]() Title: Tv20S proteasome in the complex with marizomib Authors: Silhan J / Fajtova P / O'Donoghue A / Boura E | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 323.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 33 KB 33 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 14.9 KB | Display | ![]() |
Images | ![]() | 158.7 KB | ||
Filedesc metadata | ![]() | 8.9 KB | ||
Others | ![]() ![]() | 318.2 MB 318.2 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8p0tMC ![]() 8oixC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | CryoEM map of 20S Trichomonas vaginalis proteasome in complex with proteasome inhibitor CP-17 | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.7 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: CryoEM half map A of 20S Trichomonas vaginalis...
File | emd_17337_half_map_1.map | ||||||||||||
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Annotation | CryoEM half map A of 20S Trichomonas vaginalis proteasome in complex with proteasome inhibitor CP-17 | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: CryoEM half map B of 20S Trichomonas vaginalis...
File | emd_17337_half_map_2.map | ||||||||||||
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Annotation | CryoEM half map B of 20S Trichomonas vaginalis proteasome in complex with proteasome inhibitor CP-17 | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
+Entire : Ternary complex of 20S proteasome from Trichomonas vaginalis
+Supramolecule #1: Ternary complex of 20S proteasome from Trichomonas vaginalis
+Macromolecule #1: Family T1, proteasome alpha subunit, threonine peptidase
+Macromolecule #2: Family T1, proteasome alpha subunit, threonine peptidase
+Macromolecule #3: Proteasome subunit alpha type
+Macromolecule #4: Proteasome subunit alpha type
+Macromolecule #5: Proteasome subunit alpha type
+Macromolecule #6: Family T1, proteasome alpha subunit, threonine peptidase
+Macromolecule #7: Family T1, proteasome alpha subunit, threonine peptidase
+Macromolecule #8: Proteasome subunit beta
+Macromolecule #9: proteasome endopeptidase complex
+Macromolecule #10: Proteasome subunit beta
+Macromolecule #11: Proteasome subunit beta
+Macromolecule #12: Proteasome subunit beta
+Macromolecule #13: Proteasome subunit beta
+Macromolecule #14: Family T1, proteasome beta subunit, threonine peptidase
+Macromolecule #15: N-[(2S)-1-[[(2S)-1-[[(1S)-1-[(2S,3R,5S,6R)-3-(hydroxymethyl)-5-me...
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 1 mg/mL | |||||||||
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Buffer | pH: 7.5 Component:
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Grid | Model: Quantifoil R2/1 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY ARRAY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec. | |||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Specialist optics | Energy filter - Name: TFS Selectris |
Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Number grids imaged: 1 / Number real images: 7983 / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.6 µm / Nominal defocus min: 0.8 µm |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |