- EMDB-16802: Homo sapiens Get1/Get2 heterotetramer (a3' deletion variant) in c... -
+
データを開く
IDまたはキーワード:
読み込み中...
-
基本情報
登録情報
データベース: EMDB / ID: EMD-16802
タイトル
Homo sapiens Get1/Get2 heterotetramer (a3' deletion variant) in complex with a Get3 dimer
マップデータ
試料
複合体: Homo sapiens Get1/Get2 heterotetramer (a3' deletion variant) in complex with a Get3 dimer
複合体: Tail-anchored protein insertion receptor Get1 and Get2/CAML (a3' deletion variant)
タンパク質・ペプチド: Guided entry of tail-anchored proteins factor CAMLG,Guided entry of tail-anchored proteins factor 1
複合体: Dimeric ATPase Get3
タンパク質・ペプチド: ATPase ASNA1
リガンド: ZINC ION
キーワード
membrane protein insertion / GET pathway / tail anchored membrane protein / MEMBRANE PROTEIN
機能・相同性
機能・相同性情報
arsenite transmembrane transporter activity / membrane insertase activity / GET complex / tail-anchored membrane protein insertion into ER membrane / receptor recycling / 加水分解酵素; 酸無水物に作用 / protein insertion into ER membrane / post-translational protein targeting to endoplasmic reticulum membrane / Insertion of tail-anchored proteins into the endoplasmic reticulum membrane / B cell homeostasis ...arsenite transmembrane transporter activity / membrane insertase activity / GET complex / tail-anchored membrane protein insertion into ER membrane / receptor recycling / 加水分解酵素; 酸無水物に作用 / protein insertion into ER membrane / post-translational protein targeting to endoplasmic reticulum membrane / Insertion of tail-anchored proteins into the endoplasmic reticulum membrane / B cell homeostasis / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / protein-membrane adaptor activity / vesicle-mediated transport / negative regulation of protein ubiquitination / epidermal growth factor receptor signaling pathway / defense response / protein stabilization / ubiquitin protein ligase binding / endoplasmic reticulum membrane / nucleolus / endoplasmic reticulum / signal transduction / ATP hydrolysis activity / extracellular exosome / nucleoplasm / ATP binding / membrane / nucleus / metal ion binding / cytoplasm 類似検索 - 分子機能
ジャーナル: Nat Commun / 年: 2023 タイトル: The GET insertase exhibits conformational plasticity and induces membrane thinning. 著者: Melanie A McDowell / Michael Heimes / Giray Enkavi / Ákos Farkas / Daniel Saar / Klemens Wild / Blanche Schwappach / Ilpo Vattulainen / Irmgard Sinning / 要旨: The eukaryotic guided entry of tail-anchored proteins (GET) pathway mediates the biogenesis of tail-anchored (TA) membrane proteins at the endoplasmic reticulum. In the cytosol, the Get3 chaperone ...The eukaryotic guided entry of tail-anchored proteins (GET) pathway mediates the biogenesis of tail-anchored (TA) membrane proteins at the endoplasmic reticulum. In the cytosol, the Get3 chaperone captures the TA protein substrate and delivers it to the Get1/Get2 membrane protein complex (GET insertase), which then inserts the substrate via a membrane-embedded hydrophilic groove. Here, we present structures, atomistic simulations and functional data of human and Chaetomium thermophilum Get1/Get2/Get3. The core fold of the GET insertase is conserved throughout eukaryotes, whilst thinning of the lipid bilayer occurs in the vicinity of the hydrophilic groove to presumably lower the energetic barrier of membrane insertion. We show that the gating interaction between Get2 helix α3' and Get3 drives conformational changes in both Get3 and the Get1/Get2 membrane heterotetramer. Thus, we provide a framework to understand the conformational plasticity of the GET insertase and how it remodels its membrane environment to promote substrate insertion.
全体 : Homo sapiens Get1/Get2 heterotetramer (a3' deletion variant) in c...
全体
名称: Homo sapiens Get1/Get2 heterotetramer (a3' deletion variant) in complex with a Get3 dimer
要素
複合体: Homo sapiens Get1/Get2 heterotetramer (a3' deletion variant) in complex with a Get3 dimer
複合体: Tail-anchored protein insertion receptor Get1 and Get2/CAML (a3' deletion variant)
タンパク質・ペプチド: Guided entry of tail-anchored proteins factor CAMLG,Guided entry of tail-anchored proteins factor 1
複合体: Dimeric ATPase Get3
タンパク質・ペプチド: ATPase ASNA1
リガンド: ZINC ION
-
超分子 #1: Homo sapiens Get1/Get2 heterotetramer (a3' deletion variant) in c...
超分子
名称: Homo sapiens Get1/Get2 heterotetramer (a3' deletion variant) in complex with a Get3 dimer タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1 詳細: Get2-Get1 was expressed as a fusion protein in S. frugiperda and Get3 was expressed in E. coli. The complex components were purified and reconstituted in vitro.
由来(天然)
生物種: Homo sapiens (ヒト)
-
超分子 #2: Tail-anchored protein insertion receptor Get1 and Get2/CAML (a3' ...
名称: Guided entry of tail-anchored proteins factor CAMLG,Guided entry of tail-anchored proteins factor 1 タイプ: protein_or_peptide / ID: 2 詳細: Get2-Get1 was expressed as a fusion protein in S. frugiperda コピー数: 2 / 光学異性体: LEVO