- EMDB-16224: CryoEM structure of the RAD51 nucleoprotein filament in the prese... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-16224
タイトル
CryoEM structure of the RAD51 nucleoprotein filament in the presence of ADP and Ca2+
マップデータ
Density modified map
試料
複合体: Filament of human RAD51 bound to ADP and Ca2+
タンパク質・ペプチド: DNA repair protein RAD51 homolog 1
リガンド: CALCIUM ION
リガンド: ADENOSINE-5'-DIPHOSPHATE
キーワード
DNA repair / Homologous Recombination / DNA-strand exchange / ATPase / DNA BINDING PROTEIN
機能・相同性
機能・相同性情報
presynaptic intermediate filament cytoskeleton / mitotic recombination-dependent replication fork processing / cellular response to camptothecin / chromosome organization involved in meiotic cell cycle / telomere maintenance via telomere lengthening / positive regulation of DNA ligation / nuclear ubiquitin ligase complex / double-strand break repair involved in meiotic recombination / cellular response to hydroxyurea / replication-born double-strand break repair via sister chromatid exchange ...presynaptic intermediate filament cytoskeleton / mitotic recombination-dependent replication fork processing / cellular response to camptothecin / chromosome organization involved in meiotic cell cycle / telomere maintenance via telomere lengthening / positive regulation of DNA ligation / nuclear ubiquitin ligase complex / double-strand break repair involved in meiotic recombination / cellular response to hydroxyurea / replication-born double-strand break repair via sister chromatid exchange / lateral element / DNA recombinase assembly / telomere maintenance via recombination / regulation of DNA damage checkpoint / mitotic recombination / DNA strand invasion / Impaired BRCA2 binding to PALB2 / DNA strand exchange activity / reciprocal meiotic recombination / single-stranded DNA helicase activity / Defective homologous recombination repair (HRR) due to BRCA1 loss of function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA1 binding function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA2/RAD51/RAD51C binding function / Homologous DNA Pairing and Strand Exchange / Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA) / Resolution of D-loop Structures through Holliday Junction Intermediates / ATP-dependent DNA damage sensor activity / HDR through Single Strand Annealing (SSA) / Impaired BRCA2 binding to RAD51 / regulation of double-strand break repair via homologous recombination / nuclear chromosome / condensed chromosome / DNA unwinding involved in DNA replication / replication fork processing / Transcriptional Regulation by E2F6 / Presynaptic phase of homologous DNA pairing and strand exchange / condensed nuclear chromosome / ATP-dependent activity, acting on DNA / meiotic cell cycle / male germ cell nucleus / interstrand cross-link repair / DNA polymerase binding / Meiotic recombination / cellular response to ionizing radiation / regulation of protein phosphorylation / double-strand break repair via homologous recombination / HDR through Homologous Recombination (HRR) / PML body / double-stranded DNA binding / site of double-strand break / single-stranded DNA binding / chromosome, telomeric region / DNA recombination / mitochondrial matrix / DNA repair / chromatin binding / centrosome / DNA damage response / chromatin / nucleolus / perinuclear region of cytoplasm / enzyme binding / ATP hydrolysis activity / protein-containing complex / mitochondrion / nucleoplasm / ATP binding / identical protein binding / nucleus / cytoplasm / cytosol 類似検索 - 分子機能
DNA recombination/repair protein Rad51 / DNA recombination and repair protein, RecA-like / DNA recombination and repair protein Rad51-like, C-terminal / Rad51 / DNA recombination and repair protein RecA, monomer-monomer interface / RecA family profile 2. / DNA recombination and repair protein RecA-like, ATP-binding domain / RecA family profile 1. / DNA repair Rad51/transcription factor NusA, alpha-helical / Helix-hairpin-helix domain ...DNA recombination/repair protein Rad51 / DNA recombination and repair protein, RecA-like / DNA recombination and repair protein Rad51-like, C-terminal / Rad51 / DNA recombination and repair protein RecA, monomer-monomer interface / RecA family profile 2. / DNA recombination and repair protein RecA-like, ATP-binding domain / RecA family profile 1. / DNA repair Rad51/transcription factor NusA, alpha-helical / Helix-hairpin-helix domain / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性
ジャーナル: iScience / 年: 2023 タイトル: A metal ion-dependent mechanism of RAD51 nucleoprotein filament disassembly. 著者: Robert Appleby / Daniel Bollschweiler / Dimitri Y Chirgadze / Luay Joudeh / Luca Pellegrini / 要旨: The RAD51 ATPase polymerizes on single-stranded DNA to form nucleoprotein filaments (NPFs) that are critical intermediates in the reaction of homologous recombination. ATP binding maintains the NPF ...The RAD51 ATPase polymerizes on single-stranded DNA to form nucleoprotein filaments (NPFs) that are critical intermediates in the reaction of homologous recombination. ATP binding maintains the NPF in a competent conformation for strand pairing and exchange. Once strand exchange is completed, ATP hydrolysis licenses the filament for disassembly. Here we show that the ATP-binding site of the RAD51 NPF contains a second metal ion. In the presence of ATP, the metal ion promotes the local folding of RAD51 into the conformation required for DNA binding. The metal ion is absent in the ADP-bound RAD51 filament, that rearranges in a conformation incompatible with DNA binding. The presence of the second metal ion explains how RAD51 couples the nucleotide state of the filament to DNA binding. We propose that loss of the second metal ion upon ATP hydrolysis drives RAD51 dissociation from the DNA and weakens filament stability, contributing to NPF disassembly.
全体 : Filament of human RAD51 bound to ADP and Ca2+
全体
名称: Filament of human RAD51 bound to ADP and Ca2+
要素
複合体: Filament of human RAD51 bound to ADP and Ca2+
タンパク質・ペプチド: DNA repair protein RAD51 homolog 1
リガンド: CALCIUM ION
リガンド: ADENOSINE-5'-DIPHOSPHATE
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超分子 #1: Filament of human RAD51 bound to ADP and Ca2+
超分子
名称: Filament of human RAD51 bound to ADP and Ca2+ / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: #1 詳細: Sample was prepared by mixing RAD51, dsDNA, ADP in Ca2+ buffer
由来(天然)
生物種: Homo sapiens (ヒト)
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分子 #1: DNA repair protein RAD51 homolog 1
分子
名称: DNA repair protein RAD51 homolog 1 / タイプ: protein_or_peptide / ID: 1 / コピー数: 7 / 光学異性体: LEVO