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- EMDB-16155: Structure of rabbit 80S ribosome translating beta-tubulin in comp... -

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Basic information

Entry
Database: EMDB / ID: EMD-16155
TitleStructure of rabbit 80S ribosome translating beta-tubulin in complex with tetratricopeptide protein 5 (TTC5) and S-phase Cyclin A Associated Protein residing in the ER (SCAPER)
Map dataSharpened map of the 60S subunit bound to SCAPER and TTC5
Sample
  • Complex: rabbit 80S ribosome translating beta-tubulin in complex with tetratricopeptide protein 5 (TTC5) and S-phase Cyclin A Associated Protein residing in the ER (SCAPER)
    • RNA: x 3 types
    • Protein or peptide: x 48 types
  • Ligand: x 2 types
Keywordstubulin auto-regulation mRNA decay translation SCAPER / RIBOSOME
Function / homology
Function and homology information


ooplasm / odontoblast differentiation / positive regulation of mRNA catabolic process / sperm head / cytoskeleton-dependent intracellular transport / natural killer cell mediated cytotoxicity / GTPase activating protein binding / regulation of G1 to G0 transition / exit from mitosis / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator ...ooplasm / odontoblast differentiation / positive regulation of mRNA catabolic process / sperm head / cytoskeleton-dependent intracellular transport / natural killer cell mediated cytotoxicity / GTPase activating protein binding / regulation of G1 to G0 transition / exit from mitosis / positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / regulation of translation involved in cellular response to UV / protein-DNA complex disassembly / positive regulation of DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / optic nerve development / antral ovarian follicle growth / retinal ganglion cell axon guidance / G1 to G0 transition / intercellular bridge / regulation of synapse organization / seminiferous tubule development / nuclear envelope lumen / MHC class I protein binding / microtubule-based process / positive regulation of signal transduction by p53 class mediator / ubiquitin ligase inhibitor activity / protein-RNA complex assembly / spindle assembly / cellular response to actinomycin D / rough endoplasmic reticulum / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of mitotic centrosome proteins and complexes / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest / cellular response to starvation / negative regulation of ubiquitin-dependent protein catabolic process / AURKA Activation by TPX2 / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / positive regulation of translation / Regulation of TP53 Activity through Methylation / structural constituent of cytoskeleton / cellular response to gamma radiation / mitotic spindle / mRNA 5'-UTR binding / transcription coactivator binding / cytoplasmic ribonucleoprotein granule / microtubule cytoskeleton organization / rRNA processing / antimicrobial humoral immune response mediated by antimicrobial peptide / microtubule cytoskeleton / Regulation of PLK1 Activity at G2/M Transition / azurophil granule lumen / ribosome biogenesis / ribosome binding / retina development in camera-type eye / regulation of translation / mitotic cell cycle / cell body / 5S rRNA binding / large ribosomal subunit rRNA binding / cytoplasmic vesicle / spermatogenesis / defense response to Gram-negative bacterium / microtubule / killing of cells of another organism / Potential therapeutics for SARS / nucleic acid binding / cytosolic large ribosomal subunit / tRNA binding / cytoplasmic translation / postsynaptic density / cytoskeleton / protein stabilization / rRNA binding / ribosome / nuclear speck / structural constituent of ribosome / mitochondrial matrix / ribonucleoprotein complex / translation / membrane raft / protein domain specific binding / cell division / DNA repair / GTPase activity / mRNA binding / ubiquitin protein ligase binding / synapse / DNA damage response / chromatin binding / positive regulation of cell population proliferation / Neutrophil degranulation / protein-containing complex binding / positive regulation of gene expression / nucleolus / GTP binding / structural molecule activity
Similarity search - Function
S phase cyclin A-associated protein in the endoplasmic reticulum, N-terminal / S phase cyclin A-associated protein in the endoplasmic reticulum / Tetratricopeptide repeat protein 5, OB fold domain / TTC5, OB fold domain superfamily / Tetratricopeptide repeat protein 5 OB fold domain / Zinc-finger of C2H2 type / Matrin/U1-C-like, C2H2-type zinc finger / U1-like zinc finger / 60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 ...S phase cyclin A-associated protein in the endoplasmic reticulum, N-terminal / S phase cyclin A-associated protein in the endoplasmic reticulum / Tetratricopeptide repeat protein 5, OB fold domain / TTC5, OB fold domain superfamily / Tetratricopeptide repeat protein 5 OB fold domain / Zinc-finger of C2H2 type / Matrin/U1-C-like, C2H2-type zinc finger / U1-like zinc finger / 60s Acidic ribosomal protein / 60S acidic ribosomal protein P0 / Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ribosomal protein L30e / Ribosomal protein L28e / Ribosomal L15/L27a, N-terminal / Ribosomal protein L23 / 50S ribosomal protein L10, insertion domain superfamily / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / Ribosomal protein L41 / Ribosomal protein L41 / metallochaperone-like domain / TRASH domain / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L44e signature. / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L10e / Ribosomal protein L13e / Ribosomal protein L13e / : / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L6e signature. / Ribosomal protein L30e signature 1. / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Ribosomal protein L30e signature 2. / Ribosomal protein L30e, conserved site / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L36e signature. / Ribosomal protein L39e, conserved site / Ribosomal protein L39e signature. / Ribosomal protein L34Ae / Ribosomal protein L34e / Ribosomal protein L35Ae, conserved site / Ribosomal protein L35Ae signature. / Ribosomal protein L30/YlxQ / 60S ribosomal protein L35 / Ribosomal Protein L6, KOW domain / Ribosomal protein L7A/L8 / Ribosomal protein L6e / TPR repeat region circular profile. / 60S ribosomal protein L6E / Tubulin-beta mRNA autoregulation signal. / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L4 C-terminal domain / Ribosomal protein L37ae / Beta tubulin, autoregulation binding site / Ribosomal protein L31e, conserved site / Ribosomal L37ae protein family / Ribosomal protein L31e signature. / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal protein L36e / Ribosomal protein L39e / Ribosomal protein L14e domain / Ribosomal protein L39e domain superfamily / Ribosomal L39 protein / Ribosomal protein L14 / Beta tubulin / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal large subunit proteins 60S L5, and 50S L18 / Ribosomal protein L35A / Ribosomal protein L35Ae / Ribosomal protein L35A superfamily / Ribosomal protein L32e, conserved site / Ribosomal protein L32e signature. / TPR repeat profile. / Ribosomal protein L14 / Ribosomal protein L6, conserved site-2 / Ribosomal protein L6 signature 2. / Ribosomal protein L14, KOW motif
Similarity search - Domain/homology
Small ribosomal subunit protein eS32 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL33 / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein uL29 ...Small ribosomal subunit protein eS32 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein eL24 / Large ribosomal subunit protein uL23 / Large ribosomal subunit protein eL33 / Large ribosomal subunit protein eL29 / Large ribosomal subunit protein eL31 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein uL10 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein eL8 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein eL43 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL14 / Large ribosomal subunit protein eL15 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein eL30 / Large ribosomal subunit protein eL13 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein eL39 / Large ribosomal subunit protein eL36 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein eL27 / Large ribosomal subunit protein eL38 / Large ribosomal subunit protein eL42 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein eL34 / Tubulin beta chain / Tetratricopeptide repeat protein 5 / S phase cyclin A-associated protein in the endoplasmic reticulum / Large ribosomal subunit protein eL37
Similarity search - Component
Biological speciesOryctolagus cuniculus (rabbit) / rabbit (rabbit) / Homo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.8 Å
AuthorsHopfler M / Absmeier E / Passmore LA / Hegde RS
Funding support United Kingdom, European Union, Germany, Switzerland, United States, 7 items
OrganizationGrant numberCountry
Medical Research Council (MRC, United Kingdom)MC_UP_A022_1007 United Kingdom
Medical Research Council (MRC, United Kingdom)MC_U105192715 United Kingdom
European Molecular Biology Organization (EMBO)ALTF 116-2020European Union
H2020 Marie Curie Actions of the European Commission101029853European Union
German Research Foundation (DFG)429892960 Germany
Swiss National Science FoundationPCEFP3_194312 Switzerland
Damon Runyon Cancer Research FoundationDRG:2279-16 United States
CitationJournal: Mol Cell / Year: 2023
Title: Mechanism of ribosome-associated mRNA degradation during tubulin autoregulation.
Authors: Markus Höpfler / Eva Absmeier / Sew-Yeu Peak-Chew / Evangelia Vartholomaiou / Lori A Passmore / Ivana Gasic / Ramanujan S Hegde /
Abstract: Microtubules play crucial roles in cellular architecture, intracellular transport, and mitosis. The availability of free tubulin subunits affects polymerization dynamics and microtubule function. ...Microtubules play crucial roles in cellular architecture, intracellular transport, and mitosis. The availability of free tubulin subunits affects polymerization dynamics and microtubule function. When cells sense excess free tubulin, they trigger degradation of the encoding mRNAs, which requires recognition of the nascent polypeptide by the tubulin-specific ribosome-binding factor TTC5. How TTC5 initiates the decay of tubulin mRNAs is unknown. Here, our biochemical and structural analysis reveals that TTC5 recruits the poorly studied protein SCAPER to the ribosome. SCAPER, in turn, engages the CCR4-NOT deadenylase complex through its CNOT11 subunit to trigger tubulin mRNA decay. SCAPER mutants that cause intellectual disability and retinitis pigmentosa in humans are impaired in CCR4-NOT recruitment, tubulin mRNA degradation, and microtubule-dependent chromosome segregation. Our findings demonstrate how recognition of a nascent polypeptide on the ribosome is physically linked to mRNA decay factors via a relay of protein-protein interactions, providing a paradigm for specificity in cytoplasmic gene regulation.
History
DepositionNov 17, 2022-
Header (metadata) releaseJul 5, 2023-
Map releaseJul 5, 2023-
UpdateJul 19, 2023-
Current statusJul 19, 2023Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_16155.png

Downloads & links

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Map

FileDownload / File: emd_16155.map.gz / Format: CCP4 / Size: 536.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationSharpened map of the 60S subunit bound to SCAPER and TTC5
Projections & slices

Image control

Size
Brightness
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Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.83 Å/pix.
x 520 pix.
= 431.08 Å		0.83 Å/pix.
x 520 pix.
= 431.08 Å		0.83 Å/pix.
x 520 pix.
= 431.08 Å

Surface

Projections

Slices (1/3)

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.829 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.008
Minimum - Maximum-0.027295088 - 0.064459495
Average (Standard dev.)0.00013388802 (±0.0019254888)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions520520520
Spacing520520520
CellA=B=C: 431.08 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_16155_msk_1.map
Projections & Slices
AxesZYX

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Additional map: unsharpened map of the 80S ribosome after focussed...

Fileemd_16155_additional_1.map
Annotationunsharpened map of the 80S ribosome after focussed classification around the expansion segment
Projections & Slices
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Additional map: unsharpened map of the 80S ribosome after focussed...

Fileemd_16155_additional_2.map
Annotationunsharpened map of the 80S ribosome after focussed classification around the P-site tRNA
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Additional map: unsharpened map of the 80S ribosome after focussed...

Fileemd_16155_additional_3.map
Annotationunsharpened map of the 80S ribosome after focussed classification around SCAPER
Projections & Slices
AxesZYX

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Half map: Half-map 1 of the 60S subunit bound to SCAPER and TTC5

Fileemd_16155_half_map_1.map
AnnotationHalf-map 1 of the 60S subunit bound to SCAPER and TTC5
Projections & Slices
AxesZYX

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Histogram (log scale)

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Half map: Half-map 2 of the 60S subunit bound to SCAPER and TTC5

Fileemd_16155_half_map_2.map
AnnotationHalf-map 2 of the 60S subunit bound to SCAPER and TTC5
Projections & Slices
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Sample components

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Entire : rabbit 80S ribosome translating beta-tubulin in complex with tetr...

EntireName: rabbit 80S ribosome translating beta-tubulin in complex with tetratricopeptide protein 5 (TTC5) and S-phase Cyclin A Associated Protein residing in the ER (SCAPER)
Components
  • Complex: rabbit 80S ribosome translating beta-tubulin in complex with tetratricopeptide protein 5 (TTC5) and S-phase Cyclin A Associated Protein residing in the ER (SCAPER)
    • RNA: 28S ribosomal RNA
    • RNA: 5S ribosomal RNA
    • RNA: 5.8S ribosomal RNA
    • Protein or peptide: Nascent polypeptide-associated complex subunit alpha N-terminal region
    • Protein or peptide: 60S ribosomal protein L8
    • Protein or peptide: Ribosomal protein L3
    • Protein or peptide: 60S ribosomal protein L4
    • Protein or peptide: Ribosomal_L18_c domain-containing protein
    • Protein or peptide: 60S ribosomal protein L6
    • Protein or peptide: 60S ribosomal protein L7
    • Protein or peptide: 60S ribosomal protein L7a
    • Protein or peptide: 60S ribosomal protein L9
    • Protein or peptide: Ribosomal protein L10
    • Protein or peptide: 60S ribosomal protein L11
    • Protein or peptide: 60S ribosomal protein L13
    • Protein or peptide: 60S ribosomal protein L14
    • Protein or peptide: Ribosomal protein L15
    • Protein or peptide: 60S ribosomal protein L13a
    • Protein or peptide: 60S ribosomal protein L17
    • Protein or peptide: Ribosomal protein L18
    • Protein or peptide: 60S ribosomal protein L19
    • Protein or peptide: 60S ribosomal protein L18a
    • Protein or peptide: 60S ribosomal protein L21
    • Protein or peptide: 60S ribosomal protein L22
    • Protein or peptide: 60S ribosomal protein L23
    • Protein or peptide: Ribosomal protein L24
    • Protein or peptide: Ribosomal_L23eN domain-containing protein
    • Protein or peptide: Ribosomal protein L26
    • Protein or peptide: 60S ribosomal protein L27
    • Protein or peptide: 60S ribosomal protein L27a
    • Protein or peptide: 60S ribosomal protein L29
    • Protein or peptide: 60S ribosomal protein L30
    • Protein or peptide: 60S ribosomal protein L31
    • Protein or peptide: Ribosomal protein L32
    • Protein or peptide: 60S ribosomal protein L35a
    • Protein or peptide: 60S ribosomal protein L34
    • Protein or peptide: 60S ribosomal protein L35
    • Protein or peptide: 60S ribosomal protein L36
    • Protein or peptide: Ribosomal protein L37
    • Protein or peptide: 60S ribosomal protein L38
    • Protein or peptide: 60S ribosomal protein L39-like
    • Protein or peptide: Ubiquitin-60S ribosomal protein L40
    • Protein or peptide: 60S ribosomal protein L41
    • Protein or peptide: 60S ribosomal protein L36a-like
    • Protein or peptide: 60S ribosomal protein L37a
    • Protein or peptide: 60S ribosomal protein L28
    • Protein or peptide: 60S acidic ribosomal protein P0
    • Protein or peptide: 60S ribosomal protein L12
    • Protein or peptide: S phase cyclin A-associated protein in the endoplasmic reticulum
    • Protein or peptide: Tetratricopeptide repeat protein 5
    • Protein or peptide: Tubulin beta chain
  • Ligand: MAGNESIUM ION
  • Ligand: ZINC ION

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Supramolecule #1: rabbit 80S ribosome translating beta-tubulin in complex with tetr...

SupramoleculeName: rabbit 80S ribosome translating beta-tubulin in complex with tetratricopeptide protein 5 (TTC5) and S-phase Cyclin A Associated Protein residing in the ER (SCAPER)
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#51
Source (natural)Organism: Oryctolagus cuniculus (rabbit)

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Macromolecule #1: 28S ribosomal RNA

MacromoleculeName: 28S ribosomal RNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 1.418701625 MDa
SequenceString: CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAA AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGCGGG GCGCGGGAAA UGUGGCGUAC G GAAGACCC ...String:
CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAA AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGCGGG GCGCGGGAAA UGUGGCGUAC G GAAGACCC ACUCCCCGGC GCCGGGGGGC CCAAGUCCUU CUGAUCGAGG CCCAGCCCGU GGACGGUGUG AGGCCGGUAG CG GCCCCCG GCGCGCCGGG CCCGGGUCUU CCCGGAGUCG GGUUGCUUGG GAAUGCAGCC CAAAGCGGGU GGUAAACUCC AUC UAAGGC UAAAUACCGG CACGAGACCG AUAGUCAACA AGUACCGUAA GGGAAAGUUG AAAAGAACUU UGAAGAGAGA GUUC AAGAG GGCGUGAAAC CGUUAAGAGG UAAACGGGUG GGGUCCGCGC AGUCCGCCCG GAGGAUUCAA CCCGGCGGCG CGGUC CGGC CGUGCCGGCG GGGCGGAUCU CCCGCUCCCG UCGGCGGGGG ACCGCCCCCC GGCCGGCGAC CGGCCGCCGC CGGGCG CAU UUCCACCGCG GCGGUGCGCC GCGACCGGCU CCGGGACGGC UGGGAAGGCC CCGGCGGGGA AGGUGGCCCG GGGGCCC CG GGUGUUACAG CCCCGCCCCG GCAGCAGCAG CUCGCCGAAU CCCGGGGCCG AGGGAGCGAG ACCCGUGCGC CGCGCUCU C CCCCCUCGGG GGGGCCGGGC CACCCCUCCC ACGGCGCGAC CGCUGGGGCG GACUGUCCAG UGCGCCCCGG GCGGGUCGC GCCGUCGGGC CCGGGGGGCC ACGCGUCCCC CGCGAGCGCA CGGGGUCGGC GGCGAUGUCG GCUACCCACC CGACCCGUCU UGAAACACG GACCAAGGAG UCUAACACGU GCGCGAGUCG GGGGCUACGA AAGCCGCCGU GGCGCAAUGA AGGUGAAGGC C GGCGGCGC GCCGCCGGCC GAGGUGGGAU CCCGAGGCCU UCCAGUCCGC CGAGGGCGCA CCACCGGCCC GUCUCGCCCG CC GCGCCGG GGAGGUGGAG CACGAGCGCA CGUGUUAGGA CCCGAAAGAU GGUGAACUAU GCCUGGGCAG GGCGAAGCCA GAG GAAACU CUGGUGGAGG UCCGUAGCGG UCCUGACGUG CGAAUCGGUC GUCCGACCUG GGUAUAGGGG CGAAAGACUA AUCG AACCA UCUAGUAGCU GGUUCCCUCC AAAGUUUCCC UCAGGAUAGC UGGCGCUCUC GCAACCCCGC AGUUUUAUCC GGUAA AGCG AAUGAUUAGA GGUCUUGGGG CCGAAACGAU CUCAACCUAU UCUCAAACUU UAAAUGGGUA AGAAGCCCGG CUCGCU GGU GUGGAGCCGG GCGUGGAAUG CGAGUGCCUA GUGGGCCACU UUUGGUAAGC AGAACUGGCG CUGCGGGAUG AACCGAA CG CCGGGUUAAG GCGCCCGAUG CCGACGCUCA UCAGACCCCA GAAAAGGUGU UGGUUGAUAU AGACAGCAGG AUGGUGGC C AUGGAAGUCG GAAUCCGCUA AGGAGUGUGU AACAACUCAC CUGCCGAAUC AACUAGCCCU GAAAAUGGAU GGCGCUGGA GCGUCGGGCC CAUACCCGGC CGUCGCUGGC AGUCGGCAGA GCGAGAAAGA GAGUGGAGCG CGCCGCGGGA CUCCCCCGCG GGGGUCCCG GCCACCCCCC CCGCGGACGC UACGCCGCGA CGAGUAGGAG GGCCGCUGCG GUGAGCCUUG AAGCCUAGGG C GCGGGCCC GGGUGGAGCC GCCGCAGGUG CAGAUCUUGG UGGUAGUAGC AAAUAUUCAA ACGAGAACUU UGAAGGCCGA AG UGGAGAA GGGUUCCAUG UGAACAGCAG UUGAACAUGG GUCAGUCGGU CCUGAGAGAU GGGCGAGCGC CGUUCCGAAG GGA CGGGCG AUGGCCUCCG UUGCCCUCAG CCGAUCGAAA GGGAGUCGGG UUCAGAUCCC CGAAUCCGGA GUGGCGGAGA UGGG CGCCG CGAGGCGUCC AGUGCGGUAA CGCGACCGAU CCCGGAGAAG CCGGCGGGAG CCCCGGGGAG AGUUCUCUUU UCUUU GUGA AGGGCAGGGC GCCCUGGAAU GGGUUCGCCC CGAGAGAGGG GCCCGUGCCU UGGAAAGCGU CGCGGUUCCG GCGGCG UCC GGUGAGCUCU CGCUGGCCCU UGAAAAUCCG GGGGAGAGGG UGUAAAUCUC GCGCCGGGCC GUACCCAUAU CCGCAGC AG GUCUCCAAGG UGAACAGCCU CUGGCAUGUU GGAACAAUGU AGGUAAGGGA AGUCGGCAAG CCGGAUCCGU AACUUCGG G AUAAGGAUUG GCUCUAAGGG CUGGGUCGGU CGGGCUGGGG CGCGAAGCGG GGCUGGGCGC GCGCCGCGGC UGGACGAGG CGCCGCCGCC CCCCCCACGC CCGGGCGCCC UCCCCCGGCC GGGUCCGCUC CUCGCGCGCG CACGUGCGCC GUGGCGCGCG CCGCGCGCG CGGCCGAGCC CCGUCUCCCC CCCUCUCCCC UCCCGGGGCG GGGUGCGGGG AGGGGCGGGC GGGGCCGUCG C UCGCCGCG CGUGCGCGUC GCUGCGCGUG CGCCGCGCGG GGGUCCGGCG GGCCUCCGGA CGGGGGCUCC GGGCACCCGG GC ACCCGGG GGGCCGGCGG CGGCGGCGAC UCUGGACGCG AGCCGGGCCC UUCCCGUGGA UCGCCCCAGC UGCGGCGGGC GUC GCGGCC GCCCGGGGAG CCCGGCGGGC GCCGCGUGCG CGCGCGUGCG CGCCCGGGGU CGGGGGGGUG CGAGGCAGGG GAGC CCUCU CCUCCUCCCC CACCCCGGCG CCGCGGCGCC GCGCGUGCGC GCGCGGUCUC CCCCCGCCGG GUCCGCCCCC GGGGC CGCG GUUCCGCGCG GCGCCUCGCC UCGGCCGGCG CCUAGCAGCC GACUUAGAAC UGGUGCGGAC CAGGGGAAUC CGACUG UUU AAUUAAAACA AAGCAUCGCG AAGGCCCGCG GCGGGUGUUG ACGCGAUGUG AUUUCUGCCC AGUGCUCUGA AUGUCAA AG UGAAGAAAUU CAAUGAAGCG CGGGUAAACG GCGGGAGUAA CUAUGACUCU CUUAAGGUAG CCAAAUGCCU CGUCAUCU A AUUAGUGACG CGCAUGAAUG GAUGAACGAG AUUCCCACUG UCCCUACCUA CUAUCCAGCG AAACCACAGC CAAGGGAAC GGGCUUGGCG GAAUCAGCGG GGAAAGAAGA CCCUGUUGAG CUUGACUCUA GUCUGGCACG GUGAAGAGAC AUGAGAGGUG UAGAAUAAG UGGGAGGCCC CCGCGCCCUC CCGCCCCCGC GAGGGGCGGG GCGGGGGUGC CGGCCUUGCG GGCCGCCGGU G AAAUACCA CUACUCUUAU CGUUUUUUCA CUGACCCGGU GAGGCGGGGG GGCGAGCCCC GAGGGGCUCU CGCUUCUGGC GC CAAGCGC CCGGCCCCGU CGGUCCGCGC GGGCGGCGGC CGGGCGCGAC CCGCUCCGGG GACAGUGCCA GGUGGGGAGU UUG ACUGGG GCGGUACACC UGUCAAACGG UAACGCAGGU GUCCUAAGGC GAGCUCAGGG AGGACAGAAA CCUCCCGUGG AGCA GAAGG GCAAAAGCUC GCUUGAUCUU GAUUUUCAGU ACGAAUACAG ACCGUGAAAG CGGGGCCUCA CGAUCCUUCU GACCU UUUG GGUUUUAAGC AGGAGGUGUC AGAAAAGUUA CCACAGGGAU AACUGGCUUG UGGCGGCCAA GCGUUCAUAG CGACGU CGC UUUUUGAUCC UUCGAUGUCG GCUCUUCCUA UCAUUGUGAA GCAGAAUUCA CCAAGCGUUG GAUUGUUCAC CCACUAA UA GGGAACGUGA GCUGGGUUUA GACCGUCGUG AGACAGGUUA GUUUUACCCU ACUGAUGAUG UGUUGUUGCC AUGGUAAU C CUGCUCAGUA CGAGAGGAAC CGCAGGUUCA GACAUUUGGU GUAUGUGCUU GGCUGAGGAG CCAAUGGGGC GAAGCUACC AUCUGUGGGA UUAUGACUGA ACGCCUCUAA GUCAGAAUCC CGCCCAGGCG GAACGAUAGC GCCGGAGCCU CGGUUGGCCU CGGAUAGCC GGUCCCCCGC CGUCCCCGCC GGCGGCCCGC GUCGUGCGUC CGCCUCGGCG GCGAGCGGCC CGCCCCCCGC C GCGCGUUG GGACGGGGUC CGGUGCGGAG AGCCCUUCGU CCCGGGAAAC GGGGCGGCCG GAAAGGGGGC CGCCCUCUCG CC CGUCACG CACCGCACGU UCGUGGGGAA CCUGGCGCUA AACCAUUCGU AGACGACCUG CUUCUGGGUC GGGGUUUCGU ACG UAGCAG AGCAGCUCCC UCGCUGCGAU CUAUUGAAAG UCAGCCCUCG ACACAAGGGU UUGU

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Macromolecule #2: 5S ribosomal RNA

MacromoleculeName: 5S ribosomal RNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 38.691914 KDa
SequenceString:
GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAG ACCGCCUGGG AAUACCGGGU GCUGUAGGCU U

GENBANK: GENBANK: 4CXE_4

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Macromolecule #3: 5.8S ribosomal RNA

MacromoleculeName: 5.8S ribosomal RNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 50.143648 KDa
SequenceString:
CGACUCUUAG CGGUGGAUCA CUCGGCUCGU GCGUCGAUGA AGAACGCAGC UAGCUGCGAG AAUUAAUGUG AAUUGCAGGA CACAUUGAU CAUCGACACU UCGAACGCAC UUGCGGCCCC GGGUUCCUCC CGGGGCUACG CCUGUCUGAG CGUCGCU

GENBANK: GENBANK: 4CXE_3

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Macromolecule #4: Nascent polypeptide-associated complex subunit alpha N-terminal region

MacromoleculeName: Nascent polypeptide-associated complex subunit alpha N-terminal region
type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Oryctolagus cuniculus (rabbit)
Molecular weightTheoretical: 2.526119 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) L(UNK)(UNK)(UNK)(UNK)(UNK)P (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #5: 60S ribosomal protein L8

MacromoleculeName: 60S ribosomal protein L8 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 28.088863 KDa
SequenceString: MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVY CGKKAQLNIG NVLPVGTMPE GTIVCCLEEK PGDRGKLARA SGNYATVISH NPETKKTRVK LPSGSKKVIS S ANRAVVGV ...String:
MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVY CGKKAQLNIG NVLPVGTMPE GTIVCCLEEK PGDRGKLARA SGNYATVISH NPETKKTRVK LPSGSKKVIS S ANRAVVGV VAGGGRIDKP ILKAGRAYHK YKAKRNCWPR VRGVAMNPVE HPFGGGNHQH IGKPSTIRRD APAGRKVGLI AA RRTGRLR GTKTVQEKEN

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Macromolecule #6: Ribosomal protein L3

MacromoleculeName: Ribosomal protein L3 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 46.107977 KDa
SequenceString: MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD DAGKRQLDKD FSSMKKYCQV I RVLAHTQM ...String:
MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD DAGKRQLDKD FSSMKKYCQV I RVLAHTQM RLLPLRQKKA HLMEIQVNGG TVAEKLDWAR ERLEQQVPVS QVFGQDEMID VIGVTKGKGY KGVTSRWHTK KL PRKTHRG LRKVACIGAW HPARVAFSVA RAGQKGYHHR TEINKKIYKI GQGYLIKDGK LIKNNASTDY DLSDKSINPL GGF VHYGEV TNDFVMLKGC VVGTKKRVLT LRKSLLVQTK RRALEKIDLK FIDTTSKFGH GRFQTVEEKK AFMGPLKKDR IAKE EGA

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #7: 60S ribosomal protein L4

MacromoleculeName: 60S ribosomal protein L4 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 47.727559 KDa
SequenceString: MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE G YKKTKEAV ...String:
MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE G YKKTKEAV LLLKKLKAWN DIKKVYASQR MRAGKGKMRN RRRIQRRGPC VIYNEDNGIV KAFRNIPGIT LLNVTKLNIL KL APGGHVG RFCIWTESAF RKLDDLYGTW RKAASLKSNY NLPMHKMLNT DLSRILKSPE IQRALRAPRK KIHRRVLKKN PLK NLRIML KLNPYAKTMR RNTILRQARN HKLRVERAAA ALAAKSDPKE APAKKKPVVG KKVKKPRAVG IKQKKKPVVG RKAA AAKKP AADKKAADKR AGPEDKKPAA

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #8: Ribosomal_L18_c domain-containing protein

MacromoleculeName: Ribosomal_L18_c domain-containing protein / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 34.481828 KDa
SequenceString: MGFVKVVKNK AYFKRYQVKF RRRREGKTDY YARKRLVIQD KNKYNTPKYR MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVK VGLTNYAAAY CTGLLLARRL LNRFGMDKIY EGQVEVTGDE YNVESIDGQP GAFTCYLDAG LARTTTGNKV F GALKGAVD ...String:
MGFVKVVKNK AYFKRYQVKF RRRREGKTDY YARKRLVIQD KNKYNTPKYR MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVK VGLTNYAAAY CTGLLLARRL LNRFGMDKIY EGQVEVTGDE YNVESIDGQP GAFTCYLDAG LARTTTGNKV F GALKGAVD GGLSIPHSTK RFPGYDSESK EFNAEVHRKH IMGQNVADYM RYLMEEDEDA YKKQFSQYIK NNVTPDMMEE MY KKAHAAI RENPVYEKKP KREVKKKRWN RPKMSLAQKK DRVAQKKASF LRAQERAAES

UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #9: 60S ribosomal protein L6

MacromoleculeName: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 33.028336 KDa
SequenceString: MAGEKAPAAK PDATKKSPAK KADHARGKAK KKTLAEKKPK KGKPHCSRNP VLVRGIGRYS RSAMYSRKAL YKRKYAAPKS RIERKKKRE KVLATVTKPV GGDKNGGTRV VKLRKMPRYY PTEDVPRKLL SHGKKPFSQH VRKLRASITP GTILIILTGR H RGKRVVFL ...String:
MAGEKAPAAK PDATKKSPAK KADHARGKAK KKTLAEKKPK KGKPHCSRNP VLVRGIGRYS RSAMYSRKAL YKRKYAAPKS RIERKKKRE KVLATVTKPV GGDKNGGTRV VKLRKMPRYY PTEDVPRKLL SHGKKPFSQH VRKLRASITP GTILIILTGR H RGKRVVFL KQLSSGLLLV TGPLSLNRVP LRRTHQKFVI ATSTKIDISG VKIPKHLTDA YFKKKKLRKP RHQEGEIFDT EK EKYEITE QRKVDQKAVD SQILPKIKAV PQLQGYLRSV FALTNGVYPH KLVF

UniProtKB: Large ribosomal subunit protein eL6

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Macromolecule #10: 60S ribosomal protein L7

MacromoleculeName: 60S ribosomal protein L7 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 29.201836 KDa
SequenceString: MEGAEEKKKV PAVPETLKKK RRNFAELKIK RLRKKFAQKM LRKARRKLIY EKAKHYHKEY RQMYRTEIRM ARMARKAGNF YVPAEPKLA FVIRIRGING VSPKVRKVLQ LLRLRQIFNG TFVKLNKASI NMLRIVEPYI AWGYPNLKSV NELIYKRGYG K INKKRIAL ...String:
MEGAEEKKKV PAVPETLKKK RRNFAELKIK RLRKKFAQKM LRKARRKLIY EKAKHYHKEY RQMYRTEIRM ARMARKAGNF YVPAEPKLA FVIRIRGING VSPKVRKVLQ LLRLRQIFNG TFVKLNKASI NMLRIVEPYI AWGYPNLKSV NELIYKRGYG K INKKRIAL TDNTLIARSL GKYNIICMED LIHEIYTVGK HFKEANNFLW PFKLSSPRGG MKKKTTHFVE GGDAGNREDQ IN RLIRRMN

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Macromolecule #11: 60S ribosomal protein L7a

MacromoleculeName: 60S ribosomal protein L7a / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 36.221516 KDa
SequenceString: MSSYRLGYCM KEERHNLVLC LWSQSPGILN SKCLWPFTNI HLLVGALPRE GAGGAWGGGR SEQLPTCSTT HHDFTWDKKV VNPLFEKRP KNFGIGQDIQ PKRDLTRFVK WPRYIRLQRQ RAILYKRLKV PPAINQFTQV LDRQTATQLL KLAHKYRPET K QEKKQRLL ...String:
MSSYRLGYCM KEERHNLVLC LWSQSPGILN SKCLWPFTNI HLLVGALPRE GAGGAWGGGR SEQLPTCSTT HHDFTWDKKV VNPLFEKRP KNFGIGQDIQ PKRDLTRFVK WPRYIRLQRQ RAILYKRLKV PPAINQFTQV LDRQTATQLL KLAHKYRPET K QEKKQRLL ARAEKKAAGK GDVPTKRPPV LRAGVNTVTT LVENKKAQLV VIAHDVDPIE LVVFLPALCR KMGVPYCILK GK ARLGRLV HRKTCTTVAF TQVNSEDKGA LAKLVEAIRT NYNDRYDEIR RHWGGNVLGP KSVARIAKLE KAKAKELATK LG

UniProtKB: Large ribosomal subunit protein eL8

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Macromolecule #12: 60S ribosomal protein L9

MacromoleculeName: 60S ribosomal protein L9 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 21.871418 KDa
SequenceString: MKTILSNQTV DIPENVDISL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELVLEGN DIELVSNSAA L IQQATTVK ...String:
MKTILSNQTV DIPENVDISL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELVLEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QADE

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #13: Ribosomal protein L10

MacromoleculeName: Ribosomal protein L10 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 24.643057 KDa
SequenceString: MGRRPARCYR YCKNKPYPKS RFCRGVPDAK IRIFDLGRKK AKVDEFPLCG HMVSDEYEQL SSEALEAARI CANKYMVKSC GKDGFHIRV RLHPFHVIRI NKMLSCAGAD RLQTGMRGAF GKPQGTVARV HIGQVIMSIR TKLQNKEHVV EALRRAKFKF P GRQKIHIS ...String:
MGRRPARCYR YCKNKPYPKS RFCRGVPDAK IRIFDLGRKK AKVDEFPLCG HMVSDEYEQL SSEALEAARI CANKYMVKSC GKDGFHIRV RLHPFHVIRI NKMLSCAGAD RLQTGMRGAF GKPQGTVARV HIGQVIMSIR TKLQNKEHVV EALRRAKFKF P GRQKIHIS KKWGFTKFNA DEFEDMVAEK RLIPDGCGVK YIPNRGPLDK WRALHS

UniProtKB: Large ribosomal subunit protein uL16

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Macromolecule #14: 60S ribosomal protein L11

MacromoleculeName: 60S ribosomal protein L11 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 20.288465 KDa
SequenceString:
MAQDQGEKEN PMRELRIRKL CLNICVGESG DRLTRAAKVL EQLTGQTPVF SKARYTVRSF GIRRNEKIAV HCTVRGAKAE EILEKGLKV REYELRKNNF SDTGNFGFGI QEHIDLGIKY DPSIGIYGLD FYVVLGRPGF SIADKKRRTG CIGAKHRISK E EAMRWFQQ KYDGIILPGK

UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #15: 60S ribosomal protein L13

MacromoleculeName: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 24.331723 KDa
SequenceString: MAPSRNGMIL KPHFHKDWQR RVATWFNQPA RKIRRRKARQ ARARRIAPRP AAGPIRPIVR CPTVRYHTKV RAGRGFSLEE LRVAGIHKK VARTIGISVD PRRRNKSTES LQANVQRLKE YRSKLVLFPR KPSAPKKGDS SAEELKLATQ LTGPVMPIRN V FKKEKARV ...String:
MAPSRNGMIL KPHFHKDWQR RVATWFNQPA RKIRRRKARQ ARARRIAPRP AAGPIRPIVR CPTVRYHTKV RAGRGFSLEE LRVAGIHKK VARTIGISVD PRRRNKSTES LQANVQRLKE YRSKLVLFPR KPSAPKKGDS SAEELKLATQ LTGPVMPIRN V FKKEKARV ITEEEKNFKA FASLRMARAN ARLFGIRAKR AKEAAEQDVE KKK

UniProtKB: Large ribosomal subunit protein eL13

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Macromolecule #16: 60S ribosomal protein L14

MacromoleculeName: 60S ribosomal protein L14 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 23.870549 KDa
SequenceString: MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTRVRRQAM PFKCMQLTDF ILKFPHSARQ KYVRKAWEKA DINTKWAAT RWAKKIEARE RKAKMTDFDR YKVMKAKKMR NRIIKNEVKK LQRAALLKAS PKKAPVAKGA VAAAAAAAKV P AKKATAAG ...String:
MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTRVRRQAM PFKCMQLTDF ILKFPHSARQ KYVRKAWEKA DINTKWAAT RWAKKIEARE RKAKMTDFDR YKVMKAKKMR NRIIKNEVKK LQRAALLKAS PKKAPVAKGA VAAAAAAAKV P AKKATAAG KKAAAQKAPA QKAPAQKAAG QKAAQPPKAQ KGQKPPAQKA PAPKASGKKA

UniProtKB: Large ribosomal subunit protein eL14

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Macromolecule #17: Ribosomal protein L15

MacromoleculeName: Ribosomal protein L15 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 24.207285 KDa
SequenceString: MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGAT YGKPVHHGV NQLKFARSLQ SVAEERAGRH CGALRVLNSY WVGEDSTYKF FEVILIDPFH KAIRRNPDTQ WITKPVHKHR E MRGLTSAG ...String:
MGAYKYIQEL WRKKQSDVMR FLLRVRCWQY RQLSALHRAP RPTRPDKARR LGYKAKQGYV IYRIRVRRGG RKRPVPKGAT YGKPVHHGV NQLKFARSLQ SVAEERAGRH CGALRVLNSY WVGEDSTYKF FEVILIDPFH KAIRRNPDTQ WITKPVHKHR E MRGLTSAG RKSRGLGKGH KFHHTIGGSR RAAWRRRNTL QLHRYR

UniProtKB: Large ribosomal subunit protein eL15

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Macromolecule #18: 60S ribosomal protein L13a

MacromoleculeName: 60S ribosomal protein L13a / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 23.533299 KDa
SequenceString: MAEGQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLP HKTKRGQAAL DRLKVFDGIP PPYDKKKRMV VPAALKVVRL KPTRKFAYLG RLAHEVGWKY QAVTATLEEK R KEKAKIHY ...String:
MAEGQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLP HKTKRGQAAL DRLKVFDGIP PPYDKKKRMV VPAALKVVRL KPTRKFAYLG RLAHEVGWKY QAVTATLEEK R KEKAKIHY RKKKQLMRLR KQAEKNVEKK ISKFTDVLKT HGLLV

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Macromolecule #19: 60S ribosomal protein L17

MacromoleculeName: 60S ribosomal protein L17 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 21.444221 KDa
SequenceString:
MVRYSLDPEN PTKSCKSRGS NLRVHFKNTR ETAQAIKGMH IRKATKYLKD VTLKKQCVPF RRYNGGVGRC AQAKQWGWTQ GRWPKKSAE FLLHMLKNAE SNAELKGLDV DSLVIEHIQV NKAPKMRRRT YRAHGRINPY MSSPCHIEMI LTEKEQIVPK P EEEVAQKK KISQKKLKKQ KLMARE

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Macromolecule #20: Ribosomal protein L18

MacromoleculeName: Ribosomal protein L18 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 21.568492 KDa
SequenceString:
GVDIRHNKDR KVRRKEPKSQ DIYLRLLVKL YRFLARRTNS TFNQVVLKRL FMSRTNRPPL SLSRMIRKMK LPGRENKTAV VVGTVTDDV RVQEVPKLKV CALRVTSRAR SRILKAGGKI LTFDQLALDS PKGRGTVLLS GPRKGREVYR HFGKAPGTPH S HTKPYVRS KGRKFERARG RRASRGYKN

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Macromolecule #21: 60S ribosomal protein L19

MacromoleculeName: 60S ribosomal protein L19 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 23.535281 KDa
SequenceString: MSMLRLQKRL ASSVLRCGKK KVWLDPNETN EIANANSRQQ IRKLIKDGLI IRKPVTVHSR ARCRKNTLAR RKGRHMGIGK RKGTANARM PEKVTWMRRM RILRRLLRRY RESKKIDRHM YHSLYLKVKG NVFKNKRILM EHIHKLKADK ARKKLLADQA E ARRSKTKE ...String:
MSMLRLQKRL ASSVLRCGKK KVWLDPNETN EIANANSRQQ IRKLIKDGLI IRKPVTVHSR ARCRKNTLAR RKGRHMGIGK RKGTANARM PEKVTWMRRM RILRRLLRRY RESKKIDRHM YHSLYLKVKG NVFKNKRILM EHIHKLKADK ARKKLLADQA E ARRSKTKE ARKRREERLQ AKKEEIIKTL SKEEETKK

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Macromolecule #22: 60S ribosomal protein L18a

MacromoleculeName: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 20.827561 KDa
SequenceString:
MKASGTLREY KVVGRCLPTP KCRTPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSG THNMYREYRD LTTAGAVTQC YRDMGARHRA RAHSIQIMKV EEIAASKCRR PAVKQFHDSK IKFPLPHRVL R RQHKPRFT TKRPNTFF

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Macromolecule #23: 60S ribosomal protein L21

MacromoleculeName: 60S ribosomal protein L21 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 18.609988 KDa
SequenceString:
MTNTKGKRRG TRYMFSRPFR KHGVVPLATY MRIYKKGDIV DIKGMGTVQK GMPHKCYHGK TGRVYNVTQH AVGIVVNKQV KGKILAKRI NVRIEHIKHS KSRDSFLKRV KENDQKKKEA KEKGTWVQLK RQPAPPREAH FVRTNGKEPE LLEPIPYEFM A

UniProtKB: Large ribosomal subunit protein eL21

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Macromolecule #24: 60S ribosomal protein L22

MacromoleculeName: 60S ribosomal protein L22 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 14.813015 KDa
SequenceString:
MAPVKKLVAK GGKKKKQLLK FTLDCTHPVE DGIMDAANFE QFLQERIKVN GKAGNLGGGV VSLERSKSKI TVTSEVPFSK RYLKYLTKK YLKKNNLRDW LRVVANTKES YELRYFQINQ DEEEEEEED

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Macromolecule #25: 60S ribosomal protein L23

MacromoleculeName: 60S ribosomal protein L23 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 14.892505 KDa
SequenceString:
MSKRGRGGSS GAKFRISLGL PVGAVINCAD NTGAKNLYII SVKGIKGRLN RLPAAGVGDM VMATVKKGKP ELRKKVHPAV VIRQRKSYR RKDGVFLYFE DNAGVIVNNK GEMKGSAITG PVAKECADLW PRIASNAGSI A

UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #26: Ribosomal protein L24

MacromoleculeName: Ribosomal protein L24 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 17.825111 KDa
SequenceString:
MKVELCSFSG YKIYPGHGRR YARTDGKVFQ FLNAKCESAF LSKRNPRQIN WTVLYRRKHK KGQSEEIQKK RTRRAVKFQR AITGASLAD IMAKRNQKPE VRKAQREQAI RAAKEAKKAK QASKKTAMAA AKAPTKAAPK QKIVKPVKVS APRVGGKR

UniProtKB: Large ribosomal subunit protein eL24

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Macromolecule #27: Ribosomal_L23eN domain-containing protein

MacromoleculeName: Ribosomal_L23eN domain-containing protein / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 17.768246 KDa
SequenceString:
MAPKAKKEAP APPKVEAKAK ALKAKKAVLK GVHSHKKKKI RTSPTFRRPK TLRLRRQPKY PRKSAPRRNK LDHYAIIKFP LTTESAMKK IEDNNTLVFI VDVKANKHQI KQAVKKLYDI DVAKVNTLIR PDGEKKAYVR LAPDYDALDV ANKIGII

UniProtKB: Large ribosomal subunit protein uL23

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Macromolecule #28: Ribosomal protein L26

MacromoleculeName: Ribosomal protein L26 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 17.303363 KDa
SequenceString:
MKFNPFVTSD RSKNRKRHFN APSHIRRKIM SSPLSKELRQ KYNVRSMPIR KDDEVQVVRG HYKGQQIGKV VQVYRKKYVI YIERVQREK ANGTTVHVGI HPSKVVITRL KLDKDRKKIL ERKAKSRQVG KEKGKYKEET IEKMQE

UniProtKB: Large ribosomal subunit protein uL24

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Macromolecule #29: 60S ribosomal protein L27

MacromoleculeName: 60S ribosomal protein L27 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 15.835831 KDa
SequenceString:
MGKFMKPGKV VLVLAGRYSG RKAVIVKNID DGTSDRPYSH ALVAGIDRYP RKVTAAMGKK KIAKRSKIKS FVKVYNYNHL MPTRYSVDI PLDKTVVNKD VFRDPALKRK ARREAKVKFE ERYKTGKNKW FFQKLRF

UniProtKB: Large ribosomal subunit protein eL27

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Macromolecule #30: 60S ribosomal protein L27a

MacromoleculeName: 60S ribosomal protein L27a / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 16.620561 KDa
SequenceString:
MPSRLRKTRK LRGHVSHGHG RIGKHRKHPG GRGNAGGMHH HRINFDKYHP GYFGKVGMRH YHLKRNQSFC PTVNLDKLWT LVSEQTRVN AAKNKTGAAP IIDVVRSGYY KVLGKGKLPK QPVIVKAKFF SRRAEEKIKG VGGACVLVA

UniProtKB: Large ribosomal subunit protein uL15

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Macromolecule #31: 60S ribosomal protein L29

MacromoleculeName: 60S ribosomal protein L29 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 24.931625 KDa
SequenceString: MAKSKNHTTH NQSRKWHRNG IKKPRSQRYE SLKGVDPKFL RNMRFAKKHN KKGLKKMQAN NAKAMAARAE AIKALVKPKE VKPTIPKGV SRKLHRLAYI AHPKLGRRAR ARIARGLRLS RPQTKAKAKT EPQIKGKVKA QIKAQAQAQI KSKGKGKAQA E TKPKAQAE ...String:
MAKSKNHTTH NQSRKWHRNG IKKPRSQRYE SLKGVDPKFL RNMRFAKKHN KKGLKKMQAN NAKAMAARAE AIKALVKPKE VKPTIPKGV SRKLHRLAYI AHPKLGRRAR ARIARGLRLS RPQTKAKAKT EPQIKGKVKA QIKAQAQAQI KSKGKGKAQA E TKPKAQAE TKPKAQAQAK PKAQAQGKPK AQAQGKPKAQ AQAKPKAQAQ AKPKAQAQTK PKAQATPA

UniProtKB: Large ribosomal subunit protein eL29

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Macromolecule #32: 60S ribosomal protein L30

MacromoleculeName: 60S ribosomal protein L30 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 12.807065 KDa
SequenceString:
MVAAKKTKKS LESINSRLQL VMKSGKYVLG YKQSLKMIRQ GKAKLVILAN NCPALRKSEI EYYAMLAKTG VHHYSGNNIE LGTACGKYY RVCTLSIIDP GDSDIIRSMP EQTGEK

UniProtKB: Large ribosomal subunit protein eL30

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Macromolecule #33: 60S ribosomal protein L31

MacromoleculeName: 60S ribosomal protein L31 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 14.494938 KDa
SequenceString:
MAPAKKGGEK KKGRSAINEV VTREYTINIH KRIHGVGFKK RAPRALKEIR KFAMKEMGTP DVRIDTRLNK AVWAKGIRNV PYRIRVRLS RKRNEDEDSP NKLYTLVTYV PVTTFKNLQT VNVDEN

UniProtKB: Large ribosomal subunit protein eL31

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Macromolecule #34: Ribosomal protein L32

MacromoleculeName: Ribosomal protein L32 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 15.898932 KDa
SequenceString:
MAALRPLVKP KIVKKRTKKF IRHQSDRYVK IKRNWRKPRG IDNRVRRRFK GQILMPNIGY GSNKKTKHML PSGFRKFLVH NVKELEVLL MCNKSYCAEI AHNVSSKNRK AIVERAAQLA IRVTNPNARL RSEENE

UniProtKB: Large ribosomal subunit protein eL32

+
Macromolecule #35: 60S ribosomal protein L35a

MacromoleculeName: 60S ribosomal protein L35a / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 12.580809 KDa
SequenceString:
MSGRLWCKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFR SNLPAKAIGH RIRVMLYPSR I

UniProtKB: Large ribosomal subunit protein eL33

+
Macromolecule #36: 60S ribosomal protein L34

MacromoleculeName: 60S ribosomal protein L34 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 13.196894 KDa
SequenceString:
MVQRLTYRRR LSYNTASNKT RLSRTPGNRI VYLYTKKVGK APKSACGVCP GRLRGVRAVR PKVLMRLSKT KKHVSRAYGG SMCAKCVRD RIKRAFLIEE QKIVVKVLKA QAQSQKA

UniProtKB: Large ribosomal subunit protein eL34

+
Macromolecule #37: 60S ribosomal protein L35

MacromoleculeName: 60S ribosomal protein L35 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 14.435403 KDa
SequenceString:
AKIKARDLRG KKKEELLKQL DDLKVELSQL RVAKVTGGAA SKLSKIRVVR KSIARVLTVI NQTQKENLRK FYKGKKYKPL DLRPKKTRA MRRRLNKHEE SLKTKKQQRK ERLYPLRKYA VKA

UniProtKB: Large ribosomal subunit protein uL29

+
Macromolecule #38: 60S ribosomal protein L36

MacromoleculeName: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 12.263834 KDa
SequenceString:
MALRYPMAVG LNKGHKVTKN VSKPRHSRRR GRLTKHTKFV RDMIREVCGF APYERRAMEL LKVSKDKRAL KFIKKRVGTH IRAKRKREE LSSVLAAMRK AAAKKD

UniProtKB: Large ribosomal subunit protein eL36

+
Macromolecule #39: Ribosomal protein L37

MacromoleculeName: Ribosomal protein L37 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 11.111032 KDa
SequenceString:
MTKGTSSFGK RRNKTHTLCR RCGSKAYHLQ KSTCGKCGYP AKRKRKYNWS AKAKRRNTTG TGRMRHLKIV YRRFRHGFRE GTTPKPKRA AVAASSSS

UniProtKB: Large ribosomal subunit protein eL37

+
Macromolecule #40: 60S ribosomal protein L38

MacromoleculeName: 60S ribosomal protein L38 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 8.238948 KDa
SequenceString:
MPRKIEEIKD FLLTARRKDA KSVKIKKNKD NVKFKVRCSR YLYTLVITDK EKAEKLKQSL PPGLAVKELK

UniProtKB: Large ribosomal subunit protein eL38

+
Macromolecule #41: 60S ribosomal protein L39-like

MacromoleculeName: 60S ribosomal protein L39-like / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 6.455775 KDa
SequenceString:
MSSHKTFRIK RFLAKKQKQN RPIPQWIWMK TGNKIRYNSK RRHWRRTKLG L

UniProtKB: Large ribosomal subunit protein eL39

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Macromolecule #42: Ubiquitin-60S ribosomal protein L40

MacromoleculeName: Ubiquitin-60S ribosomal protein L40 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 11.69979 KDa
SequenceString:
MGDPESGGCI PPDQQRLIFA GKQLEDGRTL SDYNIQKEST LHLVLRLRGG IIEPSLRQLA QKYNCDKMIC RKCYARLHPR AVNCRKKKC GHTNNLRPKK KVK

+
Macromolecule #43: 60S ribosomal protein L41

MacromoleculeName: 60S ribosomal protein L41 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 3.473451 KDa
SequenceString:
MRAKWRKKRM RRLKRKRRKM RQRSK

UniProtKB: Small ribosomal subunit protein eS32

+
Macromolecule #44: 60S ribosomal protein L36a-like

MacromoleculeName: 60S ribosomal protein L36a-like / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 12.476973 KDa
SequenceString:
MVNVPKTRRT FCKKCGKHQP HKVTQYKKGK DSLYAQGKRR YDRKQSGYGG QTKPIFRKKA KTTKKIVLRL ECVEPNCRSK RMLAIKRCK HFELGGDKKR KGQVIQF

UniProtKB: Large ribosomal subunit protein eL42

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Macromolecule #45: 60S ribosomal protein L37a

MacromoleculeName: 60S ribosomal protein L37a / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 10.29935 KDa
SequenceString:
MAKRTKKVGI VGKYGTRYGA SLRKMVKKIE ISQHAKYTCS FCGKTKMKRR AVGIWHCGSC MKTVAGGAWT YNTTSAVTVK SAIRRLKEL KDQ

UniProtKB: Large ribosomal subunit protein eL43

+
Macromolecule #46: 60S ribosomal protein L28

MacromoleculeName: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 15.783614 KDa
SequenceString:
MSAHLQWMVV RNCSSFLIKR NKQTYSTEPN NLKARNSFRY NGLIHRKTVG VEPAADGKGV VVVMKRRSGQ RKPATSYVRT TINKNARAT LSSIRHMIRK NKYHPDLRMA AIRRASAILR SQKPVMVKRK RTRPTKSS

UniProtKB: Large ribosomal subunit protein eL28

+
Macromolecule #47: 60S acidic ribosomal protein P0

MacromoleculeName: 60S acidic ribosomal protein P0 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 34.380504 KDa
SequenceString: MPREDRATWK SNYFLKIIQL LDDYPKCFIV GADNVGSKQM QQIRMSLRGK AVVLMGKNTM MRKAIRGHLE NNPALEKLLP HIRGNVGFV FTKEDLTEIR DMLLANKVPA AARAGAIAPC EVTVPAQNTG LGPEKTSFFQ ALGITTKISR GTIEILSDVQ L IKTGDKVG ...String:
MPREDRATWK SNYFLKIIQL LDDYPKCFIV GADNVGSKQM QQIRMSLRGK AVVLMGKNTM MRKAIRGHLE NNPALEKLLP HIRGNVGFV FTKEDLTEIR DMLLANKVPA AARAGAIAPC EVTVPAQNTG LGPEKTSFFQ ALGITTKISR GTIEILSDVQ L IKTGDKVG ASEATLLNML NISPFSFGLI IQQVFDNGSI YNPEVLDITE DTLHSRFLEG VRNVASVCLQ IGYPTVASVP HS IINGYKR VLALSVETEY TFPLAEKVKA FLADPSAFVA AAPVAAASTA APAAAAAAPA KVEAKEESEE SDEDMGFGLF D

UniProtKB: Large ribosomal subunit protein uL10

+
Macromolecule #48: 60S ribosomal protein L12

MacromoleculeName: 60S ribosomal protein L12 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: rabbit (rabbit)
Molecular weightTheoretical: 17.847619 KDa
SequenceString:
MPPKFDPNEI KVVYLRCTGG EVGATSALAP KIGPLGLSPK KVGDDIAKAT GDWKGLRITV KLTIQNRQAQ IEVVPSASAL IIKALKEPP RDRKKQKNIK HSGNITFDEI VNIARQMRHR SLARELSGTI KEILGTAQSV GCNVDGRHPH DIIDDINSGA V ECPAS

UniProtKB: Large ribosomal subunit protein uL11

+
Macromolecule #49: S phase cyclin A-associated protein in the endoplasmic reticulum

MacromoleculeName: S phase cyclin A-associated protein in the endoplasmic reticulum
type: protein_or_peptide / ID: 49
Details: First 11 N-terminal residues correspond to a FLAG-tag
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 159.795547 KDa
Recombinant expressionOrganism: Homo sapiens (human)
SequenceString: MDYKDDDDKG SMMASFQRSN SHDKVRRIVA EEGRTARNLI AWSVPLESKD DDGKPKCQTG GKSKRTIQGT HKTTKQSTAV DCKITSSTT GDKHFDKSPT KTRHPRKIDL RARYWAFLFD NLRRAVDEIY VTCESDQSVV ECKEVLMMLD NYVRDFKALI D WIQLQEKL ...String:
MDYKDDDDKG SMMASFQRSN SHDKVRRIVA EEGRTARNLI AWSVPLESKD DDGKPKCQTG GKSKRTIQGT HKTTKQSTAV DCKITSSTT GDKHFDKSPT KTRHPRKIDL RARYWAFLFD NLRRAVDEIY VTCESDQSVV ECKEVLMMLD NYVRDFKALI D WIQLQEKL EKTDAQSRPT SLAWEVKKMS PGRHVIPSPS TDRINVTSNA RRSLNFGGST GTVPAPRLAP TGVSWADKVK AH HTGSTAS SEITPAQSCP PMTVQKASRK NERKDAEGWE TVQRGRPIRS RSTAVMPKVS LATEATRSKD DSDKENVCLL PDE SIQKGQ FVGDGTSNTI ESHPKDSLHS CDHPLAEKTQ FTVSTLDDVK NSGSIRDNYV RTSEISAVHI DTECVSVMLQ AGTP PLQVN EEKFPAEKAR IENEMDPSDI SNSMAEVLAK KEELADRLEK ANEEAIASAI AEEEQLTREI EAEENNDINI ETDND SDFS ASMGSGSVSF CGMSMDWNDV LADYEARESW RQNTSWGDIV EEEPARPPGH GIHMHEKLSS PSRKRTIAES KKKHEE KQM KAQQLREKLR EEKTLKLQKL LEREKDVRKW KEELLDQRRR MMEEKLLHAE FKREVQLQAI VKKAQEEEAK VNEIAFI NT LEAQNKRHDV LSKLKEYEQR LNELQEERQR RQEEKQARDE AVQERKRALE AERQARVEEL LMKRKEQEAR IEQQRQEK E KAREDAARER ARDREERLAA LTAAQQEAME ELQKKIQLKH DESIRRHMEQ IEQRKEKAAE LSSGRHANTD YAPKLTPYE RKKQCSLCNV LISSEVYLFS HVKGRKHQQA VRENTSIQGR ELSDEEVEHL SLKKYIIDIV VESTAPAEAL KDGEERQKNK KKAKKIKAR MNFRAKEYES LMETKNSGSD SPYKAKLQRL AKDLLKQVQV QDSGSWANNK VSALDRTLGE ITRILEKENV A DQIAFQAA GGLTALEHIL QAVVPATNVN TVLRIPPKSL CNAINVYNLT CNNCSENCSD VLFSNKITFL MDLLIHQLTV YV PDENNTI LGRNTNKQVF EGLTTGLLKV SAVVLGCLIA NRPDGNCQPA TPKIPTQEMK NKPSQGDPFN NRVQDLISYV VNM GLIDKL CACFLSVQGP VDENPKMAIF LQHAAGLLHA MCTLCFAVTG RSYSIFDNNR QDPTGLTAAL QATDLAGVLH MLYC VLFHG TILDPSTASP KENYTQNTIQ VAIQSLRFFN SFAALHLPAF QSIVGAEGLS LAFRHMASSL LGHCSQVSCE SLLHE VIVC VGYFTVNHPD NQVIVQSGRH PTVLQKLCQL PFQYFSDPRL IKVLFPSLIA ACYNNHQNKI ILEQEMSCVL LATFIQ DLA QTPGQAENQP YQPKGKCLGS QDYLELANRF PQQAWEEARQ FFLKKEKK

UniProtKB: S phase cyclin A-associated protein in the endoplasmic reticulum

+
Macromolecule #50: Tetratricopeptide repeat protein 5

MacromoleculeName: Tetratricopeptide repeat protein 5 / type: protein_or_peptide / ID: 50
Details: The first 49 N-terminal residues correspond to a 6x His and a twin strep tag.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 54.176863 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MGHHHHHHEN LYFQGSAWSH PQFEKGGGSG GGSGGSAWSH PQFEKGSSGM MADEEEEVKP ILQKLQELVD QLYSFRDCYF ETHSVEDAG RKQQDVQKEM EKTLQQMEEV VGSVQGKAQV LMLTGKALNV TPDYSPKAEE LLSKAVKLEP ELVEAWNQLG E VYWKKGDV ...String:
MGHHHHHHEN LYFQGSAWSH PQFEKGGGSG GGSGGSAWSH PQFEKGSSGM MADEEEEVKP ILQKLQELVD QLYSFRDCYF ETHSVEDAG RKQQDVQKEM EKTLQQMEEV VGSVQGKAQV LMLTGKALNV TPDYSPKAEE LLSKAVKLEP ELVEAWNQLG E VYWKKGDV AAAHTCFSGA LTHCRNKVSL QNLSMVLRQL RTDTEDEHSH HVMDSVRQAK LAVQMDVHDG RSWYILGNSY LS LYFSTGQ NPKISQQALS AYAQAEKVDR KASSNPDLHL NRATLHKYEE SYGEALEGFS RAAALDPAWP EPRQREQQLL EFL DRLTSL LESKGKVKTK KLQSMLGSLR PAHLGPCSDG HYQSASGQKV TLELKPLSTL QPGVNSGAVI LGKVVFSLTT EEKV PFTFG LVDSDGPCYA VMVYNIVQSW GVLIGDSVAI PEPNLRLHRI QHKGKDYSFS SVRVETPLLL VVNGKPQGSS SQAVA TVAS RPQCE

UniProtKB: Tetratricopeptide repeat protein 5

+
Macromolecule #51: Tubulin beta chain

MacromoleculeName: Tubulin beta chain / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 7.163992 KDa
Recombinant expressionOrganism: Oryctolagus cuniculus (rabbit)
SequenceString:
MREIVHIQAG QCGNQIGAKF WEVISDEHGI DPTGTYHGDS DLQLDRISVY YNEATGGKYV MKLV

UniProtKB: Tubulin beta chain

+
Macromolecule #52: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 52 / Number of copies: 214 / Formula: MG
Molecular weightTheoretical: 24.305 Da

+
Macromolecule #53: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 53 / Number of copies: 5 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

-
Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.4
GridModel: UltrAuFoil R1.2/1.3 / Material: GOLD / Support film - Material: GRAPHENE OXIDE / Support film - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 1 / Number real images: 20932 / Average exposure time: 1.8 sec. / Average electron dose: 44.7 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.7 µm / Nominal defocus min: 1.2 µm / Nominal magnification: 105000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

Particle selectionNumber selected: 1227269
Startup modelType of model: EMDB MAP
EMDB ID:

10181

Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 18949
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 4.0)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 4.0)
Final 3D classificationSoftware - Name: RELION (ver. 4.0)
FSC plot (resolution estimation)

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