- EMDB-15308: Complex of RecO-RecR-DNA from Thermus thermophilus. -
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基本情報
登録情報
データベース: EMDB / ID: EMD-15308
タイトル
Complex of RecO-RecR-DNA from Thermus thermophilus.
マップデータ
map sharpened locally with Local Filter
試料
複合体: Complex of RecO-RecR-DNA from Thermus thermophilus.
タンパク質・ペプチド: Recombination protein RecR
タンパク質・ペプチド: DNA repair protein RecO
DNA: Oligo1
DNA: Oligo2
リガンド: ZINC ION
機能・相同性
機能・相同性情報
DNA recombination / DNA repair / DNA binding / metal ion binding 類似検索 - 分子機能
Recombination protein O, C-terminal / Recombination protein O, RecO / DNA replication/recombination mediator RecO, N-terminal / Recombination protein O C terminal / Recombination protein O N terminal / RecR, C-terminal / RecR, helix-hairpin-helix / DNA recombination protein RecR / Recombination protein RecR, conserved site / Recombination protein RecR ...Recombination protein O, C-terminal / Recombination protein O, RecO / DNA replication/recombination mediator RecO, N-terminal / Recombination protein O C terminal / Recombination protein O N terminal / RecR, C-terminal / RecR, helix-hairpin-helix / DNA recombination protein RecR / Recombination protein RecR, conserved site / Recombination protein RecR / RecR, TOPRIM domain / RecR, Cys4-zinc finger motif / RecR protein signature. / Toprim domain / ARFGAP/RecO-like zinc finger / TOPRIM / Toprim domain profile. / TOPRIM domain / Helix-hairpin-helix DNA-binding motif, class 1 / Helix-hairpin-helix DNA-binding motif class 1 / Nucleic acid-binding, OB-fold 類似検索 - ドメイン・相同性
Recombination protein RecR / DNA repair protein RecO 類似検索 - 構成要素
ジャーナル: Nat Struct Mol Biol / 年: 2023 タイトル: Mechanism of RecF-RecO-RecR cooperation in bacterial homologous recombination. 著者: Shivlee Nirwal / Mariusz Czarnocki-Cieciura / Anuradha Chaudhary / Weronika Zajko / Krzysztof Skowronek / Sebastian Chamera / Małgorzata Figiel / Marcin Nowotny / 要旨: In bacteria, one type of homologous-recombination-based DNA-repair pathway involves RecFOR proteins that bind at the junction between single-stranded (ss) and double-stranded (ds) DNA. They ...In bacteria, one type of homologous-recombination-based DNA-repair pathway involves RecFOR proteins that bind at the junction between single-stranded (ss) and double-stranded (ds) DNA. They facilitate the replacement of SSB protein, which initially covers ssDNA, with RecA, which mediates the search for homologous sequences. However, the molecular mechanism of RecFOR cooperation remains largely unknown. We used Thermus thermophilus proteins to study this system. Here, we present a cryo-electron microscopy structure of the RecF-dsDNA complex, and another reconstruction that shows how RecF interacts with two different regions of the tetrameric RecR ring. Lower-resolution reconstructions of the RecR-RecO subcomplex and the RecFOR-DNA assembly explain how RecO is positioned to interact with ssDNA and SSB, which is proposed to lock the complex on a ssDNA-dsDNA junction. Our results integrate the biochemical data available for the RecFOR system and provide a framework for its complete understanding.