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- EMDB-13164: Cryo-EM structure of encapsulin from Mycobacterium tuberculosis -
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Open data
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Basic information
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Title | Cryo-EM structure of encapsulin from Mycobacterium tuberculosis | |||||||||
![]() | LocSpiral map | |||||||||
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Function / homology | Type 1 encapsulin shell protein / Encapsulating protein for peroxidase / ![]() ![]() ![]() | |||||||||
Biological species | ![]() ![]() ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Lewis CJ / Berger C / Ravelli RBG | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Encapsulins in mycobacteria: from single particle to in situ Authors: Lewis CJ / Berger C / Gao Y / Lopez-Iglesias C / Peters PJ / Ravelli RBG | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 41.9 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 18.2 KB 18.2 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 13.5 KB | Display | ![]() |
Images | ![]() | 237.9 KB | ||
Masks | ![]() | 216 MB | ![]() | |
Others | ![]() ![]() ![]() | 40.4 MB 170.7 MB 170.7 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7p1tMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||
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Annotation | LocSpiral map | ||||||||||||||||||||
Voxel size | X=Y=Z: 0.834 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Additional map: relion postprocess masked
File | emd_13164_additional_1.map | ||||||||||||
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Annotation | relion postprocess masked | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: halfmap
File | emd_13164_half_map_1.map | ||||||||||||
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Annotation | halfmap | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: halfmap
File | emd_13164_half_map_2.map | ||||||||||||
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Annotation | halfmap | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : encapsulin shell from Mycobacterium tuberculosis
Entire | Name: encapsulin shell from Mycobacterium tuberculosis |
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Components |
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-Supramolecule #1: encapsulin shell from Mycobacterium tuberculosis
Supramolecule | Name: encapsulin shell from Mycobacterium tuberculosis / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 1.729 MDa |
Recombinant expression | Organism: ![]() ![]() ![]() |
-Macromolecule #1: 29 kDa antigen, Cfp29
Macromolecule | Name: 29 kDa antigen, Cfp29 / type: protein_or_peptide / ID: 1 / Number of copies: 60 / Enantiomer: LEVO / EC number: ![]() |
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Source (natural) | Organism: ![]() ![]() ![]() |
Molecular weight | Theoretical: 28.863346 KDa |
Recombinant expression | Organism: ![]() ![]() ![]() |
Sequence | String: MNNLYRDLAP VTEAAWAEIE LEAARTFKRH IAGRRVVDVS DPGGPVTAAV STGRLIDVKA PTNGVIAHLR ASKPLVRLRV PFTLSRNEI DDVERGSKDS DWEPVKEAAK KLAFVEDRTI FEGYSAASIE GIRSASSNPA LTLPEDPREI PDVISQALSE L RLAGVDGP ...String: MNNLYRDLAP VTEAAWAEIE LEAARTFKRH IAGRRVVDVS DPGGPVTAAV STGRLIDVKA PTNGVIAHLR ASKPLVRLRV PFTLSRNEI DDVERGSKDS DWEPVKEAAK KLAFVEDRTI FEGYSAASIE GIRSASSNPA LTLPEDPREI PDVISQALSE L RLAGVDGP YSVLLSADVY TKVSETSDHG YPIREHLNRL VDGDIIWAPA IDGAFVLTTR GGDFDLQLGT DVAIGYASHD TD TVRLYLQ ETLTFLCYTA EASVALSH |
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Concentration | 2.5 mg/mL |
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Buffer | pH: 7 |
Grid | Model: Quantifoil R1.2/1.3 / Material: GOLD / Mesh: 300 / Support film - Material: GOLD / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 90 % / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average exposure time: 1.8 sec. / Average electron dose: 40.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |