- EMDB-12478: Cryo-EM structure of the KdpFABC complex in an E1-ATP conformatio... -
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データベース: EMDB / ID: EMD-12478
タイトル
Cryo-EM structure of the KdpFABC complex in an E1-ATP conformation loaded with K+
マップデータ
KdpFABC loaded with K in a E1-ATP state at 3.1 A resolution, sharpened at -92 A2. Data obtained in presence of 5 mM AMPPCP and 50 mM KCl. Contains KdpB D307N mutation.
Netherlands Organisation for Scientific Research (NWO)
722.017.001
オランダ
Netherlands Organisation for Scientific Research (NWO)
740.018.016
オランダ
German Research Foundation (DFG)
6322/3
ドイツ
German Research Foundation (DFG)
CRC807
ドイツ
Wellcome Trust
208361/Z/17/Z
英国
引用
ジャーナル: Nat Commun / 年: 2021 タイトル: Deciphering ion transport and ATPase coupling in the intersubunit tunnel of KdpFABC. 著者: Jakob M Silberberg / Robin A Corey / Lisa Hielkema / Charlott Stock / Phillip J Stansfeld / Cristina Paulino / Inga Hänelt / 要旨: KdpFABC, a high-affinity K pump, combines the ion channel KdpA and the P-type ATPase KdpB to secure survival at K limitation. Here, we apply a combination of cryo-EM, biochemical assays, and MD ...KdpFABC, a high-affinity K pump, combines the ion channel KdpA and the P-type ATPase KdpB to secure survival at K limitation. Here, we apply a combination of cryo-EM, biochemical assays, and MD simulations to illuminate the mechanisms underlying transport and the coupling to ATP hydrolysis. We show that ions are transported via an intersubunit tunnel through KdpA and KdpB. At the subunit interface, the tunnel is constricted by a phenylalanine, which, by polarized cation-π stacking, controls K entry into the canonical substrate binding site (CBS) of KdpB. Within the CBS, ATPase coupling is mediated by the charge distribution between an aspartate and a lysine. Interestingly, individual elements of the ion translocation mechanism of KdpFABC identified here are conserved among a wide variety of P-type ATPases from different families. This leads us to the hypothesis that KdpB might represent an early descendant of a common ancestor of cation pumps.
ダウンロード / ファイル: emd_12478.map.gz / 形式: CCP4 / 大きさ: 52.7 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
KdpFABC loaded with K in a E1-ATP state at 3.1 A resolution, sharpened at -92 A2. Data obtained in presence of 5 mM AMPPCP and 50 mM KCl. Contains KdpB D307N mutation.