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基本情報
登録情報 | データベース: EMDB / ID: EMD-8495 | |||||||||
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タイトル | Subtomogram average of the piliated toxin-coregulated pilus machine in wild-type Vibrio cholerae cells (aligning IM-parts) | |||||||||
![]() | Subtomogram average of the piliated toxin-coregulated pilus machine in wild-type Vibrio cholerae cells (aligning IM-parts) | |||||||||
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生物種 | ![]() ![]() | |||||||||
手法 | サブトモグラム平均法 / クライオ電子顕微鏡法 / 解像度: 50.0 Å | |||||||||
![]() | Chang Y-W / Kjaer A / Jensen GJ | |||||||||
![]() | ![]() タイトル: Architecture of the Vibrio cholerae toxin-coregulated pilus machine revealed by electron cryotomography. 著者: Yi-Wei Chang / Andreas Kjær / Davi R Ortega / Gabriela Kovacikova / John A Sutherland / Lee A Rettberg / Ronald K Taylor / Grant J Jensen / ![]() ![]() 要旨: Type IV pili (T4P) are filamentous appendages found on many Bacteria and Archaea. They are helical fibres of pilin proteins assembled by a multi-component macromolecular machine we call the basal ...Type IV pili (T4P) are filamentous appendages found on many Bacteria and Archaea. They are helical fibres of pilin proteins assembled by a multi-component macromolecular machine we call the basal body. Based on pilin features, T4P are classified into type IVa pili (T4aP) and type IVb pili (T4bP). T4aP are more widespread and are involved in cell motility, DNA transfer, host predation and electron transfer. T4bP are less prevalent and are mainly found in enteropathogenic bacteria, where they play key roles in host colonization. Following similar work on T4aP machines, here we use electron cryotomography to reveal the three-dimensional in situ structure of a T4bP machine in its piliated and non-piliated states. The specific machine we analyse is the Vibrio cholerae toxin-coregulated pilus machine (TCPM). Although only about half of the components of the TCPM show sequence homology to components of the previously analysed Myxococcus xanthus T4aP machine (T4aPM), we find that their structures are nevertheless remarkably similar. Based on homologies with components of the M. xanthus T4aPM and additional reconstructions of TCPM mutants in which the non-homologous proteins are individually deleted, we propose locations for all eight TCPM components within the complex. Non-homologous proteins in the T4aPM and TCPM are found to form similar structures, suggesting new hypotheses for their functions and evolutionary histories. | |||||||||
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構造の表示
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構造ビューア | EMマップ: ![]() ![]() ![]() |
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マップデータ | ![]() | 988 KB | ![]() | |
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ヘッダ (付随情報) | ![]() ![]() | 10.7 KB 10.7 KB | 表示 表示 | ![]() |
画像 | ![]() | 48.6 KB | ||
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 3792C ![]() 8492C ![]() 8493C ![]() 8494C ![]() 8496C ![]() 8497C ![]() 8498C ![]() 8499C ![]() 8500C ![]() 8501C ![]() 8502C ![]() 8503C C: 同じ文献を引用 ( |
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類似構造データ | |
電子顕微鏡画像生データ | ![]() Data size: 51.3 Data #1: Different tilt series for the Vibrio cholerae toxin-coregulated pilus machine revealed by electron cryotomography [tilt series]) |
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リンク
EMDBのページ | ![]() ![]() |
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マップ
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注釈 | Subtomogram average of the piliated toxin-coregulated pilus machine in wild-type Vibrio cholerae cells (aligning IM-parts) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ボクセルのサイズ | X=Y=Z: 7.8 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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対称性 | 空間群: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
詳細 | EMDB XML:
CCP4マップ ヘッダ情報:
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-添付データ
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試料の構成要素
-全体 : Toxin-coregulated pilus machine
全体 | 名称: Toxin-coregulated pilus machine |
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要素 |
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-超分子 #1: Toxin-coregulated pilus machine
超分子 | 名称: Toxin-coregulated pilus machine / タイプ: complex / ID: 1 / 親要素: 0 |
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由来(天然) | 生物種: ![]() ![]() |
-実験情報
-構造解析
手法 | クライオ電子顕微鏡法 |
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![]() | サブトモグラム平均法 |
試料の集合状態 | cell |
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試料調製
緩衝液 | pH: 7 |
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凍結 | 凍結剤: ETHANE-PROPANE |
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電子顕微鏡法
顕微鏡 | FEI POLARA 300 |
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撮影 | フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 1.5 e/Å2 |
電子線 | 加速電圧: 300 kV / 電子線源: ![]() |
電子光学系 | 照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD |
実験機器 | ![]() モデル: Tecnai Polara / 画像提供: FEI Company |