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- EMDB-40471: Human cardiac interacting heads motif (IHM-C) in complete form -

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Basic information

Entry
Database: EMDB / ID: EMD-40471
TitleHuman cardiac interacting heads motif (IHM-C) in complete form
Map dataHuman cardiac interacting heads motif (IHM-C) in complete form
Sample
  • Complex: In situ human cardiac thick filament in the relaxed state
Keywordsmyosin / cMyBP-C / titin / STRUCTURAL PROTEIN
Biological speciesHomo sapiens (human)
Methodsubtomogram averaging / cryo EM / Resolution: 20.3 Å
AuthorsChen L / Liu J / Rastegarpouyani H / Janssen PML / Pinto JR / Taylor KA
Funding support United States, 6 items
OrganizationGrant numberCountry
National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)HL128683 United States
National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)HL160966 United States
National Institutes of Health/National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIH/NIAMS)R21 AR077802 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI087846 United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI152421 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35 GM139616 United States
CitationJournal: Proc Natl Acad Sci U S A / Year: 2024
Title: Structure of mavacamten-free human cardiac thick filaments within the sarcomere by cryoelectron tomography.
Authors: Liang Chen / Jun Liu / Hosna Rastegarpouyani / Paul M L Janssen / Jose R Pinto / Kenneth A Taylor /
Abstract: Heart muscle has the unique property that it can never rest; all cardiomyocytes contract with each heartbeat which requires a complex control mechanism to regulate cardiac output to physiological ...Heart muscle has the unique property that it can never rest; all cardiomyocytes contract with each heartbeat which requires a complex control mechanism to regulate cardiac output to physiological requirements. Changes in calcium concentration regulate the thin filament activation. A separate but linked mechanism regulates the thick filament activation, which frees sufficient myosin heads to bind the thin filament, thereby producing the required force. Thick filaments contain additional nonmyosin proteins, myosin-binding protein C and titin, the latter being the protein that transmits applied tension to the thick filament. How these three proteins interact to control thick filament activation is poorly understood. Here, we show using 3-D image reconstruction of frozen-hydrated human cardiac muscle myofibrils lacking exogenous drugs that the thick filament is structured to provide three levels of myosin activation corresponding to the three crowns of myosin heads in each 429Å repeat. In one crown, the myosin heads are almost completely activated and disordered. In another crown, many myosin heads are inactive, ordered into a structure called the interacting heads motif. At the third crown, the myosin heads are ordered into the interacting heads motif, but the stability of that motif is affected by myosin-binding protein C. We think that this hierarchy of control explains many of the effects of length-dependent activation as well as stretch activation in cardiac muscle control.
History
DepositionApr 13, 2023-
Header (metadata) releaseFeb 21, 2024-
Map releaseFeb 21, 2024-
UpdateJun 5, 2024-
Current statusJun 5, 2024Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_40471.png

Downloads & links

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Map

FileDownload / File: emd_40471.map.gz / Format: CCP4 / Size: 27 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationHuman cardiac interacting heads motif (IHM-C) in complete form
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
4.24 Å/pix.
x 192 pix.
= 813.466 Å		4.24 Å/pix.
x 192 pix.
= 813.466 Å		4.24 Å/pix.
x 192 pix.
= 813.466 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 4.2368 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 3.21
Minimum - Maximum-15.9965925 - 20.046361999999998
Average (Standard dev.)0.01076582 (±1.0002084)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin-96-96-96
Dimensions192192192
Spacing192192192
CellA=B=C: 813.46564 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: Human cardiac interacting heads motif (IHM-C) in complete...

Fileemd_40471_half_map_1.map
AnnotationHuman cardiac interacting heads motif (IHM-C) in complete form even half map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Human cardiac interacting heads motif (IHM-C) in complete...

Fileemd_40471_half_map_2.map
AnnotationHuman cardiac interacting heads motif (IHM-C) in complete form odd half map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : In situ human cardiac thick filament in the relaxed state

EntireName: In situ human cardiac thick filament in the relaxed state
Components
  • Complex: In situ human cardiac thick filament in the relaxed state

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Supramolecule #1: In situ human cardiac thick filament in the relaxed state

SupramoleculeName: In situ human cardiac thick filament in the relaxed state
type: complex / ID: 1 / Parent: 0
Source (natural)Organism: Homo sapiens (human)

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Experimental details

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Structure determination

Methodcryo EM
Processingsubtomogram averaging
Aggregation statetissue

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Sample preparation

BufferpH: 6.8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 1.5 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 6.0 µm / Nominal defocus min: 6.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 20.3 Å / Resolution method: FSC 0.143 CUT-OFF / Number subtomograms used: 1103
ExtractionNumber tomograms: 5 / Number images used: 8636
Final angle assignmentType: OTHER
FSC plot (resolution estimation)

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