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- EMDB-29263: Human nucleolar pre-60S ribosomal subunit (State H) - Composite map -

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Basic information

Entry
Database: EMDB / ID: EMD-29263
TitleHuman nucleolar pre-60S ribosomal subunit (State H) - Composite map
Map data
Sample
  • Complex: Human nucleolar pre-60S ribosomal subunit (State H)
    • Protein or peptide: x 29 types
    • RNA: x 2 types
  • Ligand: x 5 types
KeywordsPre-60S ribosomal subunit / Assembly intermediate / Ribosome / Nucleoprotein complex
Function / homology
Function and homology information


inner cell mass cell differentiation / positive regulation of protein localization to chromosome, telomeric region / basal RNA polymerase II transcription machinery binding / negative regulation of collagen binding / hematopoietic stem cell homeostasis / dendrite extension / preribosome binding / lamin filament / regulation of fatty acid biosynthetic process / regulation of megakaryocyte differentiation ...inner cell mass cell differentiation / positive regulation of protein localization to chromosome, telomeric region / basal RNA polymerase II transcription machinery binding / negative regulation of collagen binding / hematopoietic stem cell homeostasis / dendrite extension / preribosome binding / lamin filament / regulation of fatty acid biosynthetic process / regulation of megakaryocyte differentiation / positive regulation of protein sumoylation / stem cell division / miRNA-mediated post-transcriptional gene silencing / negative regulation of protein neddylation / miRNA-mediated gene silencing by inhibition of translation / regulation of G1 to G0 transition / negative regulation of formation of translation preinitiation complex / positive regulation of telomere maintenance / ribosomal protein import into nucleus / protein-DNA complex disassembly / protein localization to nucleolus / GAIT complex / G1 to G0 transition / nuclear-transcribed mRNA catabolic process / skeletal system morphogenesis / positive regulation of signal transduction by p53 class mediator / regulation of reactive oxygen species metabolic process / ubiquitin ligase inhibitor activity / positive regulation of dendritic spine development / negative regulation of ubiquitin protein ligase activity / regulation of glycolytic process / maturation of 5.8S rRNA / mitotic metaphase chromosome alignment / stem cell population maintenance / homeostatic process / lung morphogenesis / macrophage chemotaxis / negative regulation of DNA replication / negative regulation of cell-cell adhesion / ribosomal subunit export from nucleus / regulation of cyclin-dependent protein serine/threonine kinase activity / ribosomal large subunit binding / Peptide chain elongation / Selenocysteine synthesis / Formation of a pool of free 40S subunits / Eukaryotic Translation Termination / preribosome, large subunit precursor / Response of EIF2AK4 (GCN2) to amino acid deficiency / SRP-dependent cotranslational protein targeting to membrane / Viral mRNA Translation / protein localization to nucleus / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / cellular response to actinomycin D / negative regulation of mitotic cell cycle / GTP hydrolysis and joining of the 60S ribosomal subunit / negative regulation of proteasomal ubiquitin-dependent protein catabolic process / L13a-mediated translational silencing of Ceruloplasmin expression / Major pathway of rRNA processing in the nucleolus and cytosol / protein targeting / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rough endoplasmic reticulum / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / hematopoietic progenitor cell differentiation / somitogenesis / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / negative regulation of ubiquitin-dependent protein catabolic process / Notch signaling pathway / ribosomal large subunit biogenesis / negative regulation of protein ubiquitination / translation initiation factor activity / cellular response to interleukin-4 / assembly of large subunit precursor of preribosome / cytosolic ribosome / negative regulation of cell migration / cytosolic ribosome assembly / positive regulation of translation / condensed nuclear chromosome / regulation of signal transduction by p53 class mediator / kidney development / mRNA 3'-UTR binding / response to insulin / mRNA 5'-UTR binding / ribosomal large subunit assembly / transcription coactivator binding / cytoplasmic ribonucleoprotein granule / rRNA processing / osteoblast differentiation / Regulation of expression of SLITs and ROBOs / cellular response to type II interferon / positive regulation of miRNA transcription / positive regulation of canonical Wnt signaling pathway / large ribosomal subunit rRNA binding / ribosome binding / large ribosomal subunit / ribosome biogenesis / mitotic cell cycle / cell body / chromosome / cytoplasmic translation
Similarity search - Function
Coiled-coil domain-containing protein 86 / Ribosome biogenesis protein Rpf2 / Learning associated protein 18-like / Learning-associated protein / Cgr1-like / Cgr1 family / Ribosomal biogenesis regulatory protein / Ribosome biogenesis regulatory protein (RRS1) / Nucleolar GTP-binding protein 2, N-terminal domain / Nucleolar GTP-binding protein 2 ...Coiled-coil domain-containing protein 86 / Ribosome biogenesis protein Rpf2 / Learning associated protein 18-like / Learning-associated protein / Cgr1-like / Cgr1 family / Ribosomal biogenesis regulatory protein / Ribosome biogenesis regulatory protein (RRS1) / Nucleolar GTP-binding protein 2, N-terminal domain / Nucleolar GTP-binding protein 2 / NGP1NT (NUC091) domain / NLE / NLE (NUC135) domain / Guanine nucleotide-binding protein-like 3, N-terminal domain / GNL3L/Grn1 putative GTPase / Ribosomal protein L24e / GTP-binding protein, orthogonal bundle domain superfamily / Ribosomal biogenesis NSA2 family / Ribosome assembly factor Mrt4 / NOG, C-terminal / Nucleolar GTP-binding protein 1 / NOGCT (NUC087) domain / Nucleolar GTP-binding protein 1, Rossman-fold domain / NOG1, N-terminal helical domain / Nucleolar GTP-binding protein 1 (NOG1) / NOG1 N-terminal helical domain / Brix domain / Brix domain / Brix domain profile. / Brix / Circularly permuted (CP)-type guanine nucleotide-binding (G) domain / Circularly permuted (CP)-type guanine nucleotide-binding (G) domain profile. / Ribosomal protein L6, N-terminal / OBG-type guanine nucleotide-binding (G) domain / OBG-type guanine nucleotide-binding (G) domain profile. / Ribosomal protein L6, N-terminal domain / Translation initiation factor IF6 / eIF-6 family / translation initiation factor 6 / Ribosomal protein L28e / 50S ribosome-binding GTPase / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / GTP binding domain / 50S ribosomal protein L10, insertion domain superfamily / 60S ribosomal protein L10P, insertion domain / Insertion domain in 60S ribosomal protein L10P / metallochaperone-like domain / TRASH domain / : / Ribosomal protein L24e, conserved site / Ribosomal protein L35Ae, conserved site / Ribosomal Protein L6, KOW domain / Ribosomal protein L14e domain / 60S ribosomal protein L6E / Ribosomal protein L35A / Ribosomal protein L35A superfamily / Ribosomal protein L32e, conserved site / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L7, eukaryotic / Ribosomal protein L6e / Ribosomal protein L14 / Ribosomal protein L30, N-terminal / Ribosomal protein L6, conserved site-2 / Ribosomal protein L14, KOW motif / Ribosomal L30 N-terminal domain / Ribosomal protein L14 / Ribosomal protein L35Ae / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / 60S ribosomal protein L4 C-terminal domain / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L24e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / Ribosomal protein L6e signature. / Ribosomal protein L21e / Ribosomal protein L21e, conserved site / Ribosomal protein L21 superfamily / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L4/L1e, eukaryotic/archaeal, conserved site / Ribosomal protein L3, domain 3, archaeal type superfamily / Ribosomal protein L3, archaeal/eukaryotic type / Ribosomal protein L21e / Ribosomal protein L32e / Ribosomal protein L32e superfamily / Ribosomal_L32e / Ribosomal protein L24e-related / Ribosomal protein L24e/L24 superfamily / Ribosomal protein L35Ae signature. / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L4, eukaryotic and archaeal type / Ribosomal protein L18e / Ribosomal protein L24e / Ribosomal protein L32 / Ribosomal protein L7, eukaryotic/archaeal / Ribosomal protein L7/L30
Similarity search - Domain/homology
Ribosome biogenesis protein NSA2 homolog / Large ribosomal subunit protein eL33 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL21 ...Ribosome biogenesis protein NSA2 homolog / Large ribosomal subunit protein eL33 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL11 / Large ribosomal subunit protein uL6 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein eL28 / Large ribosomal subunit protein eL14 / Eukaryotic translation initiation factor 6 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein eL32 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein eL20 / Large ribosomal subunit protein eL6 / Large ribosomal subunit protein eL18 / Nucleolar GTP-binding protein 2 / Ribosome biogenesis regulatory protein homolog / Protein LLP homolog / Guanine nucleotide-binding protein-like 3 / GTP-binding protein 4 / Coiled-coil domain-containing protein 86 / Ribosome production factor 2 homolog / Notchless protein homolog 1 / Probable ribosome biogenesis protein RLP24 / mRNA turnover protein 4 homolog
Similarity search - Component
Biological speciesHomo sapiens (human) / human (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.91 Å
AuthorsVanden Broeck A / Klinge S
Funding supportEuropean Union, United States, 2 items
OrganizationGrant numberCountry
European Molecular Biology Organization (EMBO)ALTF 711-2019European Union
The G. Harold and Leila Y. Mathers FoundationMF-2104-01554 United States
CitationJournal: Science / Year: 2023
Title: Principles of human pre-60 biogenesis.
Authors: Arnaud Vanden Broeck / Sebastian Klinge /
Abstract: During the early stages of human large ribosomal subunit (60) biogenesis, an ensemble of assembly factors establishes and fine-tunes the essential RNA functional centers of pre-60 particles by an ...During the early stages of human large ribosomal subunit (60) biogenesis, an ensemble of assembly factors establishes and fine-tunes the essential RNA functional centers of pre-60 particles by an unknown mechanism. Here, we report a series of cryo-electron microscopy structures of human nucleolar and nuclear pre-60 assembly intermediates at resolutions of 2.5 to 3.2 angstroms. These structures show how protein interaction hubs tether assembly factor complexes to nucleolar particles and how guanosine triphosphatases and adenosine triphosphatase couple irreversible nucleotide hydrolysis steps to the installation of functional centers. Nuclear stages highlight how a conserved RNA-processing complex, the rixosome, couples large-scale RNA conformational changes with pre-ribosomal RNA processing by the RNA degradation machinery. Our ensemble of human pre-60 particles provides a rich foundation with which to elucidate the molecular principles of ribosome formation.
History
DepositionDec 21, 2022-
Header (metadata) releaseJul 12, 2023-
Map releaseJul 12, 2023-
UpdateJul 19, 2023-
Current statusJul 19, 2023Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_29263.png

Downloads & links

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Map

FileDownload / File: emd_29263.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.072 Å
Density
Contour LevelBy AUTHOR: 0.9
Minimum - Maximum-1.6377656 - 6.5792446
Average (Standard dev.)0.021820514 (±0.12314983)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions480480480
Spacing480480480
CellA=B=C: 514.56 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_29263_msk_1.map
Projections & Slices
AxesZYX

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Slices (1/2)
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Half map: #2

Fileemd_29263_half_map_1.map
Projections & Slices
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Half map: #1

Fileemd_29263_half_map_2.map
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Sample components

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Entire : Human nucleolar pre-60S ribosomal subunit (State H)

EntireName: Human nucleolar pre-60S ribosomal subunit (State H)
Components
  • Complex: Human nucleolar pre-60S ribosomal subunit (State H)
    • Protein or peptide: 60S ribosomal protein L12
    • RNA: 28S rRNA28S ribosomal RNA
    • RNA: 5S rRNA5S ribosomal RNA
    • Protein or peptide: 60S ribosomal protein L11
    • Protein or peptide: 60S ribosomal protein L13a
    • Protein or peptide: 60S ribosomal protein L14
    • Protein or peptide: 60S ribosomal protein L18
    • Protein or peptide: 60S ribosomal protein L18a
    • Protein or peptide: 60S ribosomal protein L21
    • Protein or peptide: 60S ribosomal protein L23
    • Protein or peptide: 60S ribosomal protein L28
    • Protein or peptide: 60S ribosomal protein L3
    • Protein or peptide: 60S ribosomal protein L32
    • Protein or peptide: 60S ribosomal protein L35a
    • Protein or peptide: Guanine nucleotide-binding protein-like 3
    • Protein or peptide: Nucleolar GTP-binding protein 2
    • Protein or peptide: Ribosome production factor 2 homolog
    • Protein or peptide: Ribosome biogenesis protein NSA2 homolog
    • Protein or peptide: Notchless protein homolog 1
    • Protein or peptide: Protein LLP homolog
    • Protein or peptide: Coiled-coil domain-containing protein 86
    • Protein or peptide: 60S ribosomal protein L4
    • Protein or peptide: 60S ribosomal protein L5
    • Protein or peptide: 60S ribosomal protein L6
    • Protein or peptide: 60S ribosomal protein L7
    • Protein or peptide: 60S ribosomal protein L9
    • Protein or peptide: Eukaryotic translation initiation factor 6
    • Protein or peptide: mRNA turnover protein 4 homolog
    • Protein or peptide: GTP-binding protein 4
    • Protein or peptide: Ribosome biogenesis regulatory protein homolog
    • Protein or peptide: Probable ribosome biogenesis protein RLP24
  • Ligand: MAGNESIUM ION
  • Ligand: GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate
  • Ligand: POTASSIUM IONPotassium
  • Ligand: GUANOSINE-5'-DIPHOSPHATE
  • Ligand: ZINC ION

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Supramolecule #1: Human nucleolar pre-60S ribosomal subunit (State H)

SupramoleculeName: Human nucleolar pre-60S ribosomal subunit (State H) / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#31
Source (natural)Organism: Homo sapiens (human) / Strain: HEK293F

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Macromolecule #1: 60S ribosomal protein L12

MacromoleculeName: 60S ribosomal protein L12 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 17.847619 KDa
SequenceString:
MPPKFDPNEI KVVYLRCTGG EVGATSALAP KIGPLGLSPK KVGDDIAKAT GDWKGLRITV KLTIQNRQAQ IEVVPSASAL IIKALKEPP RDRKKQKNIK HSGNITFDEI VNIARQMRHR SLARELSGTI KEILGTAQSV GCNVDGRHPH DIIDDINSGA V ECPAS

UniProtKB: Large ribosomal subunit protein uL11

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Macromolecule #4: 60S ribosomal protein L11

MacromoleculeName: 60S ribosomal protein L11 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 20.288465 KDa
SequenceString:
MAQDQGEKEN PMRELRIRKL CLNICVGESG DRLTRAAKVL EQLTGQTPVF SKARYTVRSF GIRRNEKIAV HCTVRGAKAE EILEKGLKV REYELRKNNF SDTGNFGFGI QEHIDLGIKY DPSIGIYGLD FYVVLGRPGF SIADKKRRTG CIGAKHRISK E EAMRWFQQ KYDGIILPGK

UniProtKB: Large ribosomal subunit protein uL5

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Macromolecule #5: 60S ribosomal protein L13a

MacromoleculeName: 60S ribosomal protein L13a / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 23.633412 KDa
SequenceString: MAEVQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLP HKTKRGQAAL DRLKVFDGIP PPYDKKKRMV VPAALKVVRL KPTRKFAYLG RLAHEVGWKY QAVTATLEEK R KEKAKIHY ...String:
MAEVQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLP HKTKRGQAAL DRLKVFDGIP PPYDKKKRMV VPAALKVVRL KPTRKFAYLG RLAHEVGWKY QAVTATLEEK R KEKAKIHY RKKKQLMRLR KQAEKNVEKK IDKYTEVLKT HGLLV

UniProtKB: Large ribosomal subunit protein uL13

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Macromolecule #6: 60S ribosomal protein L14

MacromoleculeName: 60S ribosomal protein L14 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 23.485016 KDa
SequenceString: MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTQVRRQAM PFKCMQLTDF ILKFPHSAHQ KYVRQAWQKA DINTKWAAT RWAKKIEARE RKAKMTDFDR FKVMKAKKMR NRIIKNEVKK LQKAALLKAS PKKAPGTKGT AAAAAAAAAA K VPAKKITA ...String:
MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTQVRRQAM PFKCMQLTDF ILKFPHSAHQ KYVRQAWQKA DINTKWAAT RWAKKIEARE RKAKMTDFDR FKVMKAKKMR NRIIKNEVKK LQKAALLKAS PKKAPGTKGT AAAAAAAAAA K VPAKKITA ASKKAPAQKV PAQKATGQKA APAPKAQKGQ KAPAQKAPAP KASGKKA

UniProtKB: Large ribosomal subunit protein eL14

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Macromolecule #7: 60S ribosomal protein L18

MacromoleculeName: 60S ribosomal protein L18 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 21.687676 KDa
SequenceString:
MGVDIRHNKD RKVRRKEPKS QDIYLRLLVK LYRFLARRTN STFNQVVLKR LFMSRTNRPP LSLSRMIRKM KLPGRENKTA VVVGTITDD VRVQEVPKLK VCALRVTSRA RSRILRAGGK ILTFDQLALD SPKGCGTVLL SGPRKGREVY RHFGKAPGTP H SHTKPYVR SKGRKFERAR GRRASRGYKN

UniProtKB: Large ribosomal subunit protein eL18

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Macromolecule #8: 60S ribosomal protein L18a

MacromoleculeName: 60S ribosomal protein L18a / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 20.808514 KDa
SequenceString:
MKASGTLREY KVVGRCLPTP KCHTPPLYRM RIFAPNHVVA KSRFWYFVSQ LKKMKKSSGE IVYCGQVFEK SPLRVKNFGI WLRYDSRSG THNMYREYRD LTTAGAVTQC YRDMGARHRA RAHSIQIMKV EEIAASKCRR PAVKQFHDSK IKFPLPHRVL R RQHKPRFT TKRPNTFF

UniProtKB: Large ribosomal subunit protein eL20

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Macromolecule #9: 60S ribosomal protein L21

MacromoleculeName: 60S ribosomal protein L21 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 18.609988 KDa
SequenceString:
MTNTKGKRRG TRYMFSRPFR KHGVVPLATY MRIYKKGDIV DIKGMGTVQK GMPHKCYHGK TGRVYNVTQH AVGIVVNKQV KGKILAKRI NVRIEHIKHS KSRDSFLKRV KENDQKKKEA KEKGTWVQLK RQPAPPREAH FVRTNGKEPE LLEPIPYEFM A

UniProtKB: Large ribosomal subunit protein eL21

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Macromolecule #10: 60S ribosomal protein L23

MacromoleculeName: 60S ribosomal protein L23 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 14.892505 KDa
SequenceString:
MSKRGRGGSS GAKFRISLGL PVGAVINCAD NTGAKNLYII SVKGIKGRLN RLPAAGVGDM VMATVKKGKP ELRKKVHPAV VIRQRKSYR RKDGVFLYFE DNAGVIVNNK GEMKGSAITG PVAKECADLW PRIASNAGSI A

UniProtKB: Large ribosomal subunit protein uL14

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Macromolecule #11: 60S ribosomal protein L28

MacromoleculeName: 60S ribosomal protein L28 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 15.784622 KDa
SequenceString:
MSAHLQWMVV RNCSSFLIKR NKQTYSTEPN NLKARNSFRY NGLIHRKTVG VEPAADGKGV VVVIKRRSGQ RKPATSYVRT TINKNARAT LSSIRHMIRK NKYRPDLRMA AIRRASAILR SQKPVMVKRK RTRPTKSS

UniProtKB: Large ribosomal subunit protein eL28

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Macromolecule #12: 60S ribosomal protein L3

MacromoleculeName: 60S ribosomal protein L3 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 46.224133 KDa
SequenceString: MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD EDGKKQLEKD FSSMKKYCQV I RVIAHTQM ...String:
MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV DRPGSKVNKK EVVEAVTIVE TPPMVVVGI VGYVETPRGL RTFKTVFAEH ISDECKRRFY KNWHKSKKKA FTKYCKKWQD EDGKKQLEKD FSSMKKYCQV I RVIAHTQM RLLPLRQKKA HLMEIQVNGG TVAEKLDWAR ERLEQQVPVN QVFGQDEMID VIGVTKGKGY KGVTSRWHTK KL PRKT(HIC)RG LRKVACIGAW HPARVAFSVA RAGQKGYHHR TEINKKIYKI GQGYLIKDGK LIKNNASTDY DLSDKSINP LGGFVHYGEV TNDFVMLKGC VVGTKKRVLT LRKSLLVQTK RRALEKIDLK FIDTTSKFGH GRFQTMEEKK AFMGPLKKDR IAKEEGA

UniProtKB: Large ribosomal subunit protein uL3

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Macromolecule #13: 60S ribosomal protein L32

MacromoleculeName: 60S ribosomal protein L32 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 15.898932 KDa
SequenceString:
MAALRPLVKP KIVKKRTKKF IRHQSDRYVK IKRNWRKPRG IDNRVRRRFK GQILMPNIGY GSNKKTKHML PSGFRKFLVH NVKELEVLL MCNKSYCAEI AHNVSSKNRK AIVERAAQLA IRVTNPNARL RSEENE

UniProtKB: Large ribosomal subunit protein eL32

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Macromolecule #14: 60S ribosomal protein L35a

MacromoleculeName: 60S ribosomal protein L35a / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 12.564743 KDa
SequenceString:
MSGRLWSKAI FAGYKRGLRN QREHTALLKI EGVYARDETE FYLGKRCAYV YKAKNNTVTP GGKPNKTRVI WGKVTRAHGN SGMVRAKFR SNLPAKAIGH RIRVMLYPSR I

UniProtKB: Large ribosomal subunit protein eL33

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Macromolecule #15: Guanine nucleotide-binding protein-like 3

MacromoleculeName: Guanine nucleotide-binding protein-like 3 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 62.098242 KDa
SequenceString: MKRPKLKKAS KRMTCHKRYK IQKKVREHHR KLRKEAKKRG HKKPRKDPGV PNSAPFKEAL LREAELRKQR LEELKQQQKL DRQKELEKK RKLETNPDIK PSNVEPMEKE FGLCKTENKA KSGKQNSKKL YCQELKKVIE ASDVVLEVLD ARDPLGCRCP Q VEEAIVQS ...String:
MKRPKLKKAS KRMTCHKRYK IQKKVREHHR KLRKEAKKRG HKKPRKDPGV PNSAPFKEAL LREAELRKQR LEELKQQQKL DRQKELEKK RKLETNPDIK PSNVEPMEKE FGLCKTENKA KSGKQNSKKL YCQELKKVIE ASDVVLEVLD ARDPLGCRCP Q VEEAIVQS GQKKLVLILN KSDLVPKENL ESWLNYLKKE LPTVVFRAST KPKDKGKITK RVKAKKNAAP FRSEVCFGKE GL WKLLGGF QETCSKAIRV GVIGFPNVGK SSIINSLKQE QMCNVGVSMG LTRSMQVVPL DKQITIIDSP SFIVSPLNSS SAL ALRSPA SIEVVKPMEA ASAILSQADA RQVVLKYTVP GYRNSLEFFT VLAQRRGMHQ KGGIPNVEGA AKLLWSEWTG ASLA YYCHP PTSWTPPPYF NESIVVDMKS GFNLEELEKN NAQSIRAIKG PHLANSILFQ SSGLTNGIIE EKDIHEELPK RKERK QEER EDDKDSDQET VDEEVDENSS GMFAAEETGE ALSEETTAGE QSTRSFILDK IIEEDDAYDF STDYV

UniProtKB: Guanine nucleotide-binding protein-like 3

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Macromolecule #16: Nucleolar GTP-binding protein 2

MacromoleculeName: Nucleolar GTP-binding protein 2 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 83.796094 KDa
SequenceString: MVKPKYKGRS TINPSKASTN PDRVQGAGGQ NMRDRATIRR LNMYRQKERR NSRGKIIKPL QYQSTVASGT VARVEPNIKW FGNTRVIKQ SSLQKFQEEM DTVMKDPYKV VMKQSKLPMS LLHDRIRPHN LKVHILDTES FETTFGPKSQ RKRPNLFASD M QSLIENAE ...String:
MVKPKYKGRS TINPSKASTN PDRVQGAGGQ NMRDRATIRR LNMYRQKERR NSRGKIIKPL QYQSTVASGT VARVEPNIKW FGNTRVIKQ SSLQKFQEEM DTVMKDPYKV VMKQSKLPMS LLHDRIRPHN LKVHILDTES FETTFGPKSQ RKRPNLFASD M QSLIENAE MSTESYDQGK DRDLVTEDTG VRNEAQEEIY KKGQSKRIWG ELYKVIDSSD VVVQVLDARD PMGTRSPHIE TY LKKEKPW KHLIFVLNKC DLVPTWATKR WVAVLSQDYP TLAFHASLTN PFGKGAFIQL LRQFGKLHTD KKQISVGFIG YPN VGKSSV INTLRSKKVC NVAPIAGETK VWQYITLMRR IFLIDCPGVV YPSEDSETDI VLKGVVQVEK IKSPEDHIGA VLER AKPEY ISKTYKIDSW ENAEDFLEKL AFRTGKLLKG GEPDLQTVGK MVLNDWQRGR IPFFVKPPNA EPLVAPQLLP SSSLE VVPE AAQNNPGEEV TETAGEGSES IIKEETEENS HCDANTEMQQ ILTRVRQNFG KINVVPQFSG DDLVPVEVSD LEEELE SFS DEEEEEQEQQ RDDAEESSSE PEEENVGNDT KAVIKALDEK IAKYQKFLDK AKAKKFSAVR ISKGLSEKIF AKPEEQR KT LEEDVDDRAP SKKGKKRKAQ REEEQEHSNK APRALTSKER RRAVRQQRPK KVGVRYYETH NVKNRNRNKK KTNDSEGQ K HKRKKFRQKQ

UniProtKB: Nucleolar GTP-binding protein 2

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Macromolecule #17: Ribosome production factor 2 homolog

MacromoleculeName: Ribosome production factor 2 homolog / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 35.658078 KDa
SequenceString: MDTLDRVVKP KTKRAKRFLE KREPKLNENI KNAMLIKGGN ANATVTKVLK DVYALKKPYG VLYKKKNITR PFEDQTSLEF FSKKSDCSL FMFGSHNKKR PNNLVIGRMY DYHVLDMIEL GIENFVSLKD IKNSKCPEGT KPMLIFAGDD FDVTEDYRRL K SLLIDFFR ...String:
MDTLDRVVKP KTKRAKRFLE KREPKLNENI KNAMLIKGGN ANATVTKVLK DVYALKKPYG VLYKKKNITR PFEDQTSLEF FSKKSDCSL FMFGSHNKKR PNNLVIGRMY DYHVLDMIEL GIENFVSLKD IKNSKCPEGT KPMLIFAGDD FDVTEDYRRL K SLLIDFFR GPTVSNIRLA GLEYVLHFTA LNGKIYFRSY KLLLKKSGCR TPRIELEEMG PSLDLVLRRT HLASDDLYKL SM KMPKALK PKKKKNISHD TFGTTYGRIH MQKQDLSKLQ TRKMKGLKKR PAERITEDHE KKSKRIKKN

UniProtKB: Ribosome production factor 2 homolog

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Macromolecule #18: Ribosome biogenesis protein NSA2 homolog

MacromoleculeName: Ribosome biogenesis protein NSA2 homolog / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 30.136703 KDa
SequenceString: MPQNEYIELH RKRYGYRLDY HEKKRKKESR EAHERSKKAK KMIGLKAKLY HKQRHAEKIQ MKKTIKMHEK RNTKQKNDEK TPQGAVPAY LLDREGQSRA KVLSNMIKQK RKEKAGKWEV PLPKVRAQGE TEVLKVIRTG KRKKKAWKRM VTKVCFVGDG F TRKPPKYE ...String:
MPQNEYIELH RKRYGYRLDY HEKKRKKESR EAHERSKKAK KMIGLKAKLY HKQRHAEKIQ MKKTIKMHEK RNTKQKNDEK TPQGAVPAY LLDREGQSRA KVLSNMIKQK RKEKAGKWEV PLPKVRAQGE TEVLKVIRTG KRKKKAWKRM VTKVCFVGDG F TRKPPKYE RFIRPMGLRF KKAHVTHPEL KATFCLPILG VKKNPSSPLY TTLGVITKGT VIEVNVSELG LVTQGGKVIW GK YAQVTNN PENDGCINAV LLV

UniProtKB: Ribosome biogenesis protein NSA2 homolog

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Macromolecule #19: Notchless protein homolog 1

MacromoleculeName: Notchless protein homolog 1 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 53.387141 KDa
SequenceString: MAAAVPDEAV ARDVQRLLVQ FQDEGGQLLG SPFDVPVDIT PDRLQLVCNA LLAQEDPLPL AFFVHDAEIV SSLGKTLESQ AVETEKVLD IIYQPQAIFR VRAVTRCTSS LEGHSEAVIS VAFSPTGKYL ASGSGDTTVR FWDLSTETPH FTCKGHRHWV L SISWSPDG ...String:
MAAAVPDEAV ARDVQRLLVQ FQDEGGQLLG SPFDVPVDIT PDRLQLVCNA LLAQEDPLPL AFFVHDAEIV SSLGKTLESQ AVETEKVLD IIYQPQAIFR VRAVTRCTSS LEGHSEAVIS VAFSPTGKYL ASGSGDTTVR FWDLSTETPH FTCKGHRHWV L SISWSPDG RKLASGCKNG QILLWDPSTG KQVGRTLAGH SKWITGLSWE PLHANPECRY VASSSKDGSV RIWDTTAGRC ER ILTGHTQ SVTCLRWGGD GLLYSASQDR TIKVWRAHDG VLCRTLQGHG HWVNTMALST DYALRTGAFE PAEASVNPQD LQG SLQELK ERALSRYNLV RGQGPERLVS GSDDFTLFLW SPAEDKKPLT RMTGHQALIN QVLFSPDSRI VASASFDKSI KLWD GRTGK YLASLRGHVA AVYQIAWSAD SRLLVSGSSD STLKVWDVKA QKLAMDLPGH ADEVYAVDWS PDGQRVASGG KDKCL RIWR R

UniProtKB: Notchless protein homolog 1

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Macromolecule #20: Protein LLP homolog

MacromoleculeName: Protein LLP homolog / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 15.268361 KDa
SequenceString:
MAKSLRSKWK RKMRAEKRKK NAPKEASRLK SILKLDGDVL MKDVQEIATV VVPKPKHCQE KMQCEVKDEK DDMKMETDIK RNKKTLLDQ HGQYPIWMNQ RQRKRLKAKR EKRKGKSKAK AVKVAKGLAW

UniProtKB: Protein LLP homolog

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Macromolecule #21: Coiled-coil domain-containing protein 86

MacromoleculeName: Coiled-coil domain-containing protein 86 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 40.312742 KDa
SequenceString: MDTPLRRSRR LGGLRPESPE SLTSVSRTRR ALVEFESNPE ETREPGSPPS VQRAGLGSPE RPPKTSPGSP RLQQGAGLES PQGQPEPGA ASPQRQQDLH LESPQRQPEY SPESPRCQPK PSEEAPKCSQ DQGVLASELA QNKEELTPGA PQHQLPPVPG S PEPYPGQQ ...String:
MDTPLRRSRR LGGLRPESPE SLTSVSRTRR ALVEFESNPE ETREPGSPPS VQRAGLGSPE RPPKTSPGSP RLQQGAGLES PQGQPEPGA ASPQRQQDLH LESPQRQPEY SPESPRCQPK PSEEAPKCSQ DQGVLASELA QNKEELTPGA PQHQLPPVPG S PEPYPGQQ APGPEPSQPL LELTPRAPGS PRGQHEPSKP PPAGETVTGG FGAKKRKGSS SQAPASKKLN KEELPVIPKG KP KSGRVWK DRSKKRFSQM LQDKPLRTSW QRKMKERQER KLAKDFARHL EEEKERRRQE KKQRRAENLK RRLENERKAE VVQ VIRNPA KLKRAKKKQL RSIEKRDTLA LLQKQPPQQP AAKI

UniProtKB: Coiled-coil domain-containing protein 86

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Macromolecule #22: 60S ribosomal protein L4

MacromoleculeName: 60S ribosomal protein L4 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 47.804621 KDa
SequenceString: MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE G YKKTKEAV ...String:
MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH QTSAESWGTG RAVARIPRVR GGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE G YKKTKEAV LLLKKLKAWN DIKKVYASQR MRAGKGKMRN RRRIQRRGPC IIYNEDNGII KAFRNIPGIT LLNVSKLNIL KL APGGHVG RFCIWTESAF RKLDELYGTW RKAASLKSNY NLPMHKMINT DLSRILKSPE IQRALRAPRK KIHRRVLKKN PLK NLRIML KLNPYAKTMR RNTILRQARN HKLRVDKAAA AAAALQAKSD EKAAVAGKKP VVGKKGKKAA VGVKKQKKPL VGKK AAATK KPAPEKKPAE KKPTTEEKKP AA

UniProtKB: Large ribosomal subunit protein uL4

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Macromolecule #23: 60S ribosomal protein L5

MacromoleculeName: 60S ribosomal protein L5 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 34.426789 KDa
SequenceString: MGFVKVVKNK AYFKRYQVKF RRRREGKTDY YARKRLVIQD KNKYNTPKYR MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVK VGLTNYAAAY CTGLLLARRL LNRFGMDKIY EGQVEVTGDE YNVESIDGQP GAFTCYLDAG LARTTTGNKV F GALKGAVD ...String:
MGFVKVVKNK AYFKRYQVKF RRRREGKTDY YARKRLVIQD KNKYNTPKYR MIVRVTNRDI ICQIAYARIE GDMIVCAAYA HELPKYGVK VGLTNYAAAY CTGLLLARRL LNRFGMDKIY EGQVEVTGDE YNVESIDGQP GAFTCYLDAG LARTTTGNKV F GALKGAVD GGLSIPHSTK RFPGYDSESK EFNAEVHRKH IMGQNVADYM RYLMEEDEDA YKKQFSQYIK NSVTPDMMEE MY KKAHAAI RENPVYEKKP KKEVKKKRWN RPKMSLAQKK DRVAQKKASF LRAQERAAES

UniProtKB: Large ribosomal subunit protein uL18

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Macromolecule #24: 60S ribosomal protein L6

MacromoleculeName: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 32.810176 KDa
SequenceString: MAGEKVEKPD TKEKKPEAKK VDAGGKVKKG NLKAKKPKKG KPHCSRNPVL VRGIGRYSRS AMYSRKAMYK RKYSAAKSKV EKKKKEKVL ATVTKPVGGD KNGGTRVVKL RKMPRYYPTE DVPRKLLSHG KKPFSQHVRK LRASITPGTI LIILTGRHRG K RVVFLKQL ...String:
MAGEKVEKPD TKEKKPEAKK VDAGGKVKKG NLKAKKPKKG KPHCSRNPVL VRGIGRYSRS AMYSRKAMYK RKYSAAKSKV EKKKKEKVL ATVTKPVGGD KNGGTRVVKL RKMPRYYPTE DVPRKLLSHG KKPFSQHVRK LRASITPGTI LIILTGRHRG K RVVFLKQL ASGLLLVTGP LVLNRVPLRR THQKFVIATS TKIDISNVKI PKHLTDAYFK KKKLRKPRHQ EGEIFDTEKE KY EITEQRK IDQKAVDSQI LPKIKAIPQL QGYLRSVFAL TNGIYPHKLV F

UniProtKB: Large ribosomal subunit protein eL6

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Macromolecule #25: 60S ribosomal protein L7

MacromoleculeName: 60S ribosomal protein L7 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 29.290973 KDa
SequenceString: MEGVEEKKKE VPAVPETLKK KRRNFAELKI KRLRKKFAQK MLRKARRKLI YEKAKHYHKE YRQMYRTEIR MARMARKAGN FYVPAEPKL AFVIRIRGIN GVSPKVRKVL QLLRLRQIFN GTFVKLNKAS INMLRIVEPY IAWGYPNLKS VNELIYKRGY G KINKKRIA ...String:
MEGVEEKKKE VPAVPETLKK KRRNFAELKI KRLRKKFAQK MLRKARRKLI YEKAKHYHKE YRQMYRTEIR MARMARKAGN FYVPAEPKL AFVIRIRGIN GVSPKVRKVL QLLRLRQIFN GTFVKLNKAS INMLRIVEPY IAWGYPNLKS VNELIYKRGY G KINKKRIA LTDNALIARS LGKYGIICME DLIHEIYTVG KRFKEANNFL WPFKLSSPRG GMKKKTTHFV EGGDAGNRED QI NRLIRRM N

UniProtKB: Large ribosomal subunit protein uL30

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Macromolecule #26: 60S ribosomal protein L9

MacromoleculeName: 60S ribosomal protein L9 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 21.899471 KDa
SequenceString: MKTILSNQTV DIPENVDITL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELILEGN DIELVSNSAA L IQQATTVK ...String:
MKTILSNQTV DIPENVDITL KGRTVIVKGP RGTLRRDFNH INVELSLLGK KKKRLRVDKW WGNRKELATV RTICSHVQNM IKGVTLGFR YKMRSVYAHF PINVVIQENG SLVEIRNFLG EKYIRRVRMR PGVACSVSQA QKDELILEGN DIELVSNSAA L IQQATTVK NKDIRKFLDG IYVSEKGTVQ QADE

UniProtKB: Large ribosomal subunit protein uL6

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Macromolecule #27: Eukaryotic translation initiation factor 6

MacromoleculeName: Eukaryotic translation initiation factor 6 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 26.62001 KDa
SequenceString: MAVRASFENN CEIGCFAKLT NTYCLVAIGG SENFYSVFEG ELSDTIPVVH ASIAGCRIIG RMCVGNRHGL LVPNNTTDQE LQHIRNSLP DTVQIRRVEE RLSALGNVTT CNDYVALVHP DLDRETEEIL ADVLKVEVFR QTVADQVLVG SYCVFSNQGG L VHPKTSIE ...String:
MAVRASFENN CEIGCFAKLT NTYCLVAIGG SENFYSVFEG ELSDTIPVVH ASIAGCRIIG RMCVGNRHGL LVPNNTTDQE LQHIRNSLP DTVQIRRVEE RLSALGNVTT CNDYVALVHP DLDRETEEIL ADVLKVEVFR QTVADQVLVG SYCVFSNQGG L VHPKTSIE DQDELSSLLQ VPLVAGTVNR GSEVIAAGMV VNDWCAFCGL DTTSTELSVV ESVFKLNEAQ PSTIATSMRD SL IDSLT

UniProtKB: Eukaryotic translation initiation factor 6

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Macromolecule #28: mRNA turnover protein 4 homolog

MacromoleculeName: mRNA turnover protein 4 homolog / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 27.602535 KDa
SequenceString: MPKSKRDKKV SLTKTAKKGL ELKQNLIEEL RKCVDTYKYL FIFSVANMRN SKLKDIRNAW KHSRMFFGKN KVMMVALGRS PSDEYKDNL HQVSKRLRGE VGLLFTNRTK EEVNEWFTKY TEMDYARAGN KAAFTVSLDP GPLEQFPHSM EPQLRQLGLP T ALKRGVVT ...String:
MPKSKRDKKV SLTKTAKKGL ELKQNLIEEL RKCVDTYKYL FIFSVANMRN SKLKDIRNAW KHSRMFFGKN KVMMVALGRS PSDEYKDNL HQVSKRLRGE VGLLFTNRTK EEVNEWFTKY TEMDYARAGN KAAFTVSLDP GPLEQFPHSM EPQLRQLGLP T ALKRGVVT LLSDYEVCKE GDVLTPEQAR VLKLFGYEMA EFKVTIKYMW DSQSGRFQQM GDDLPESASE STEESDSEDD D

UniProtKB: mRNA turnover protein 4 homolog

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Macromolecule #29: GTP-binding protein 4

MacromoleculeName: GTP-binding protein 4 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 74.10782 KDa
SequenceString: MAHYNFKKIT VVPSAKDFID LTLSKTQRKT PTVIHKHYQI HRIRHFYMRK VKFTQQNYHD RLSQILTDFP KLDDIHPFYA DLMNILYDK DHYKLALGQI NIAKNLVDNV AKDYVRLMKY GDSLYRCKQL KRAALGRMCT VIKRQKQSLE YLEQVRQHLS R LPTIDPNT ...String:
MAHYNFKKIT VVPSAKDFID LTLSKTQRKT PTVIHKHYQI HRIRHFYMRK VKFTQQNYHD RLSQILTDFP KLDDIHPFYA DLMNILYDK DHYKLALGQI NIAKNLVDNV AKDYVRLMKY GDSLYRCKQL KRAALGRMCT VIKRQKQSLE YLEQVRQHLS R LPTIDPNT RTLLLCGYPN VGKSSFINKV TRADVDVQPY AFTTKSLFVG HMDYKYLRWQ VVDTPGILDH PLEDRNTIEM QA ITALAHL RAAVLYVMDL SEQCGHGLRE QLELFQNIRP LFINKPLIVV ANKCDVKRIA ELSEDDQKIF TDLQSEGFPV IET STLTEE GVIKVKTEAC DRLLAHRVET KMKGNKVNEV LNRLHLAIPT RRDDKERPPF IPEGVVARRK RMETEESRKK RERD LELEM GDDYILDLQK YWDLMNLSEK HDKIPEIWEG HNIADYIDPA IMKKLEELEK EEELRTAAGE YDSVSESEDE EMLEI RQLA KQIREKKKLK ILESKEKNTQ GPRMPRTAKK VQRTVLEKEM RSLGVDMDDK DDAHYAVQAR RSRSITRKRK REDSAP PSS VARSGSCSRT PRDVSGLRDV KMVKKAKTMM KNAQKKMNRL GKKGEADRHV FDMKPKHLLS GKRKAGKKDR R

UniProtKB: GTP-binding protein 4

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Macromolecule #30: Ribosome biogenesis regulatory protein homolog

MacromoleculeName: Ribosome biogenesis regulatory protein homolog / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 41.278523 KDa
SequenceString: MEGQSVEELL AKAEQDEAEK LQRITVHKEL ELQFDLGNLL ASDRNPPTGL RCAGPTPEAE LQALARDNTQ LLINQLWQLP TERVEEAIV ARLPEPTTRL PREKPLPRPR PLTRWQQFAR LKGIRPKKKT NLVWDEVSGQ WRRRWGYQRA RDDTKEWLIE V PGNADPLE ...String:
MEGQSVEELL AKAEQDEAEK LQRITVHKEL ELQFDLGNLL ASDRNPPTGL RCAGPTPEAE LQALARDNTQ LLINQLWQLP TERVEEAIV ARLPEPTTRL PREKPLPRPR PLTRWQQFAR LKGIRPKKKT NLVWDEVSGQ WRRRWGYQRA RDDTKEWLIE V PGNADPLE DQFAKRIQAK KERVAKNELN RLRNLARAHK MQLPSAAGLH PTGHQSKEEL GRAMQVAKVS TASVGRFQER LP KEKVPRG SGKKRKFQPL FGDFAAEKKN QLELLRVMNS KKPQLDVTRA TNKQMREEDQ EEAAKRRKMS QKGKRKGGRQ GPG GKRKGG PPSQGGKRKG GLGGKMNSGP PGLGGKRKGG QRPGGKRRK

UniProtKB: Ribosome biogenesis regulatory protein homolog

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Macromolecule #31: Probable ribosome biogenesis protein RLP24

MacromoleculeName: Probable ribosome biogenesis protein RLP24 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: human (human)
Molecular weightTheoretical: 19.666258 KDa
SequenceString:
MRIEKCYFCS GPIYPGHGMM FVRNDCKVFR FCKSKCHKNF KKKRNPRKVR WTKAFRKAAG KELTVDNSFE FEKRRNEPIK YQRELWNKT IDAMKRVEEI KQKRQAKFIM NRLKKNKELQ KVQDIKEVKQ NIHLIRAPLA GKGKQLEEKM VQQLQEDVDM E DAP

UniProtKB: Probable ribosome biogenesis protein RLP24

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Macromolecule #2: 28S rRNA

MacromoleculeName: 28S rRNA / type: rna / ID: 2 / Number of copies: 1
Source (natural)Organism: human (human)
Molecular weightTheoretical: 1.640222125 MDa
SequenceString: CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAG AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGCGGG GCGCGGGACA UGUGGCGUAC G GAAGACCC ...String:
CGCGACCUCA GAUCAGACGU GGCGACCCGC UGAAUUUAAG CAUAUUAGUC AGCGGAGGAG AAGAAACUAA CCAGGAUUCC CUCAGUAAC GGCGAGUGAA CAGGGAAGAG CCCAGCGCCG AAUCCCCGCC CCGCGGCGGG GCGCGGGACA UGUGGCGUAC G GAAGACCC GCUCCCCGGC GCCGCUCGUG GGGGGCCCAA GUCCUUCUGA UCGAGGCCCA GCCCGUGGAC GGUGUGAGGC CG GUAGCGG CCCCCGGCGC GCCGGGCCCG GGUCUUCCCG GAGUCGGGUU GCUUGGGAAU GCAGCCCAAA GCGGGUGGUA AAC UCCAUC UAAGGCUAAA UACCGGCACG AGACCGAUAG UCAACAAGUA CCGUAAGGGA AAGUUGAAAA GAACUUUGAA GAGA GAGUU CAAGAGGGCG UGAAACCGUU AAGAGGUAAA CGGGUGGGGU CCGCGCAGUC CGCCCGGAGG AUUCAACCCG GCGGC GGGU CCGGCCGUGU CGGCGGCCCG GCGGAUCUUU CCCGCCCCCC GUUCCUCCCG ACCCCUCCAC CCGCCCUCCC UUCCCC CGC CGCCCCUCCU CCUCCUCCCC GGAGGGGGCG GGCUCCGGCG GGUGCGGGGG UGGGCGGGCG GGGCCGGGGG UGGGGUC GG CGGGGGACCG UCCCCCGACC GGCGACCGGC CGCCGCCGGG CGCAUUUCCA CCGCGGCGGU GCGCCGCGAC CGGCUCCG G GACGGCUGGG AAGGCCCGGC GGGGAAGGUG GCUCGGGGGG CCCCGUCCGU CCGUCCGUCC GUCCUCCUCC UCCCCCGUC UCCGCCCCCC GGCCCCGCGU CCUCCCUCGG GAGGGCGCGC GGGUCGGGGC GGCGGCGGCG GCGGCGGUGG CGGCGGCGGC GGCGGCGGC GGGACCGAAA CCCCCCCCGA GUGUUACAGC CCCCCCGGCA GCAGCACUCG CCGAAUCCCG GGGCCGAGGG A GCGAGACC CGUCGCCGCG CUCUCCCCCC UCCCGGCGCC CACCCCCGCG GGGAAUCCCC CGCGAGGGGG GUCUCCCCCG CG GGGGCGC GCCGGCGUCU CCUCGUGGGG GGGCCGGGCC ACCCCUCCCA CGGCGCGACC GCUCUCCCAC CCCUCCUCCC CGC GCCCCC GCCCCGGCGA CGGGGGGGGU GCCGCGCGCG GGUCGGGGGG CGGGGCGGAC UGUCCCCAGU GCGCCCCGGG CGGG UCGCG CCGUCGGGCC CGGGGGAGGU UCUCUCGGGG CCACGCGCGC GUCCCCCGAA GAGGGGGACG GCGGAGCGAG CGCAC GGGG UCGGCGGCGA CGUCGGCUAC CCACCCGACC CGUCUUGAAA CACGGACCAA GGAGUCUAAC ACGUGCGCGA GUCGGG GGC UCGCACGAAA GCCGCCGUGG CGCAAUGAAG GUGAAGGCCG GCGCGCUCGC CGGCCGAGGU GGGAUCCCGA GGCCUCU CC AGUCCGCCGA GGGCGCACCA CCGGCCCGUC UCGCCCGCCG CGCCGGGGAG GUGGAGCACG AGCGCACGUG UUAGGACC C GAAAGAUGGU GAACUAUGCC UGGGCAGGGC GAAGCCAGAG GAAACUCUGG UGGAGGUCCG UAGCGGUCCU GACGUGCAA AUCGGUCGUC CGACCUGGGU AUAGGGGCGA AAGACUAAUC GAACCAUCUA GUAGCUGGUU CCCUCCGAAG UUUCCCUCAG GAUAGCUGG CGCUCUCGCA GACCCGACGC ACCCCCGCCA CGCAGUUUUA UCCGGUAAAG CGAAUGAUUA GAGGUCUUGG G GCCGAAAC GAUCUCAACC UAUUCUCAAA CUUUAAAUGG GUAAGAAGCC CGGCUCGCUG GCGUGGAGCC GGGCGUGGAA UG CGAGUGC CUAGUGGGCC ACUUUUGGUA AGCAGAACUG GCGCUGCGGG AUGAACCGAA CGCCGGGUUA AGGCGCCCGA UGC CGACGC UCAUCAGACC CCAGAAAAGG UGUUGGUUGA UAUAGACAGC AGGACGGUGG CCAUGGAAGU CGGAAUCCGC UAAG GAGUG UGUAACAACU CACCUGCCGA AUCAACUAGC CCUGAAAAUG GAUGGCGCUG GAGCGUCGGG CCCAUACCCG GCCGU CGCC GGCAGUCGAG AGUGGACGGG AGCGGCGGGG GCGGCGCGCG CGCGCGCGCG UGUGGUGUGC GUCGGAGGGC GGCGGC GGC GGCGGCGGCG GGGGUGUGGG GUCCUUCCCC CGCCCCCCCC CCCACGCCUC CUCCCCUCCU CCCGCCCACG CCCCGCU CC CCGCCCCCGG AGCCCCGCGG ACGCUACGCC GCGACGAGUA GGAGGGCCGC UGCGGUGAGC CUUGAAGCCU AGGGCGCG G GCCCGGGUGG AGCCGCCGCA GGUGCAGAUC UUGGUGGUAG UAGCAAAUAU UCAAACGAGA ACUUUGAAGG CCGAAGUGG AGAAGGGUUC CAUGUGAACA GCAGUUGAAC AUGGGUCAGU CGGUCCUGAG AGAUGGGCGA GCGCCGUUCC GAAGGGACGG GCGAUGGCC UCCGUUGCCC UCGGCCGAUC GAAAGGGAGU CGGGUUCAGA UCCCCGAAUC CGGAGUGGCG GAGAUGGGCG C CGCGAGGC GUCCAGUGCG GUAACGCGAC CGAUCCCGGA GAAGCCGGCG GGAGCCCCGG GGAGAGUUCU CUUUUCUUUG UG AAGGGCA GGGCGCCCUG GAAUGGGUUC GCCCCGAGAG AGGGGCCCGU GCCUUGGAAA GCGUCGCGGU UCCGGCGGCG UCC GGUGAG CUCUCGCUGG CCCUUGAAAA UCCGGGGGAG AGGGUGUAAA UCUCGCGCCG GGCCGUACCC AUAUCCGCAG CAGG UCUCC AAGGUGAACA GCCUCUGGCA UGUUGGAACA AUGUAGGUAA GGGAAGUCGG CAAGCCGGAU CCGUAACUUC GGGAU AAGG AUUGGCUCUA AGGGCUGGGU CGGUCGGGCU GGGGCGCGAA GCGGGGCUGG GCGCGCGCCG CGGCUGGACG AGGCGC CGC CGCCCCCCCC ACGCCCGGGG CACCCCCCUC GCGGCCCUCC CCCGCCCCAC CCCGCGCGCG CCGCUCGCUC CCUCCCC GC CCCGCGCCCU CUCUCUCUCU CUCUCCCCCG CUCCCCGUCC UCCCCCCUCC CCGGGGGAGC GCCGCGUGGG GGCGGCGG C GGGGGGAGAA GGGUCGGGGC GGCAGGGGCC GGCGGCGGCC CGCCGCGGGG CCCCGGCGGC GGGGGCACGG UCCCCCGCG AGGGGGGCCC GGGCACCCGG GGGGCCGGCG GCGGCGGCGA CUCUGGACGC GAGCCGGGCC CUUCCCGUGG AUCGCCCCAG CUGCGGCGG GCGUCGCGGC CGCCCCCGGG GAGCCCGGCG GGCGCCGGCG CGCCCCCCCC CCCACCCCAC GUCUCGUCGC G CGCGCGUC CGCUGGGGGC GGGGAGCGGU CGGGCGGCGG CGGUCGGCGG GCGGCGGGGC GGGGCGGUUC GUCCCCCCGC CC UACCCCC CCGGCCCCGU CCGCCCCCCG UUCCCCCCUC CUCCUCGGCG CGCGGCGGCG GCGGCGGCAG GCGGCGGAGG GGC CGCGGG CCGGUCCCCC CCGCCGGGUC CGCCCCCGGG GCCGCGGUUC CGCGCGGCGC CUCGCCUCGG CCGGCGCCUA GCAG CCGAC UUAGAACUGG UGCGGACCAG GGGAAUCCGA CUGUUUAAUU AAAACAAAGC AUCGCGAAGG CCCGCGGCGG GUGUU GACG CGAUGUGAUU UCUGCCCAGU GCUCUGAAUG UCAAAGUGAA GAAAUUCAAU GAAGCGCGGG UAAACGGCGG GAGUAA CUA UGACUCUCUU AAGGUAGCCA AAUGCCUCGU CAUCUAAUUA GUGACGCGCA UGAAUGGAUG AACGAGAUUC CCACUGU CC CUACCUACUA UCCAGCGAAA CCACAGCCAA GGGAACGGGC UUGGCGGAAU CAGCGGGGAA AGAAGACCCU GUUGAGCU U GACUCUAGUC UGGCACGGUG AAGAGACAUG AGAGGUGUAG AAUAAGUGGG AGGCCCCCGG CGCCCCCCCG GUGUCCCCG CGAGGGGCCC GGGGCGGGGU CCGCCGGCCC UGCGGGCCGC CGGUGAAAUA CCACUACUCU GAUCGUUUUU UCACUGACCC GGUGAGGCG GGGGGGCGAG CCCCGAGGGG CUCUCGCUUC UGGCGCCAAG CGCCCGGCCG CGCGCCGGCC GGGCGCGACC C GCUCCGGG GACAGUGCCA GGUGGGGAGU UUGACUGGGG CGGUACACCU GUCAAACGGU AACGCAGGUG UCCUAAGGCG AG CUCAGGG AGGACAGAAA CCUCCCGUGG AGCAGAAGGG CAAAAGCUCG CUUGAUCUUG AUUUUCAGUA CGAAUACAGA CCG UGAAAG CGGGGCCUCA CGAUCCUUCU GACCUUUUGG GUUUUAAGCA GGAGGUGUCA GAAAAGUUAC CACAGGGAUA ACUG GCUUG UGGCGGCCAA GCGUUCAUAG CGACGUCGCU UUUUGAUCCU UCGAUGUCGG CUCUUCCUAU CAUUGUGAAG CAGAA UUCA CCAAGCGUUG GAUUGUUCAC CCACUAAUAG GGAACGUGAG CUGGGUUUAG ACCGUCGUGA GACAGGUUAG UUUUAC CCU ACUGAUGAUG UGUUGUUGCC AUGGUAAUCC UGCUCAGUAC GAGAGGAACC GCAGGUUCAG ACAUUUGGUG UAUGUGC UU GGCUGAGGAG CCAAUGGGGC GAAGCUACCA UCUGUGGGAU UAUGACUGAA CGCCUCUAAG UCAGAAUCCC GCCCAGGC G GAACGAUACG GCAGCGCCGC GGAGCCUCGG UUGGCCUCGG AUAGCCGGUC CCCCGCCUGU CCCCGCCGGC GGGCCGCCC CCCCCCUCCA CGCGCCCCGC GCGCGCGGGA GGGCGCGUGC CCCGCCGCGC GCCGGGACCG GGGUCCGGUG CGGAGUGCCC UUCGUCCUG GGAAACGGGG CGCGGCCGGA GAGGCGGCCG CCCCCUCGCC CGUCACGCAC CGCACGUUCG UGGGGAACCU G GCGCUAAA CCAUUCGUAG ACGACCUGCU UCUGGGUCGG GGUUUCGUAC GUAGCAGAGC AGCUCCCUCG CUGCGAUCUA UU GAAAGUC AGCCCUCGAC ACAAGGGUUU GUC

GENBANK: GENBANK: AL592188.60

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Macromolecule #3: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: human (human)
Molecular weightTheoretical: 38.998078 KDa
SequenceString:
GUCUACGGCC AUACCACCCU GAACGCGCCC GAUCUCGUCU GAUCUCGGAA GCUAAGCAGG GUCGGGCCUG GUUAGUACUU GGAUGGGAG ACCGCCUGGG AAUACCGGGU GCUGUAGGCU UU

GENBANK: GENBANK: X12811.1

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Macromolecule #32: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 32 / Number of copies: 22 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #33: GUANOSINE-5'-TRIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 33 / Number of copies: 1 / Formula: GTP
Molecular weightTheoretical: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

+
Macromolecule #34: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 34 / Number of copies: 2 / Formula: K
Molecular weightTheoretical: 39.098 Da

+
Macromolecule #35: GUANOSINE-5'-DIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 35 / Number of copies: 1 / Formula: GDP
Molecular weightTheoretical: 443.201 Da
Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

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Macromolecule #36: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 36 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 7.6
GridModel: Quantifoil R3.5/1 / Material: GOLD / Mesh: 400 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 2 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 283 K / Instrument: FEI VITROBOT MARK IV
Details: Four applications with manual blotting before last blotting with the vitrobot..

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 64000
Specialist opticsEnergy filter - Slit width: 20 eV
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 4 / Number real images: 172699 / Average exposure time: 2.0 sec. / Average electron dose: 60.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

-
Image processing

Particle selectionNumber selected: 15679142
Startup modelType of model: INSILICO MODEL
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 4.0)
Final 3D classificationSoftware: (Name: RELION (ver. 4.0), cryoSPARC (ver. 3.3.2))
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 3.3.2)
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.91 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 3.3.2) / Number images used: 67272
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: REAL
Output model

PDB-8fl0:
Human nucleolar pre-60S ribosomal subunit (State H)

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