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- EMDB-25395: Goslar chimallin C4 tetramer localized reconstruction -

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Basic information

Entry
Database: EMDB / ID: EMD-25395
TitleGoslar chimallin C4 tetramer localized reconstruction
Map data
Sample
  • Complex: Goslar chimallin tetramer localized reconstruction
    • Protein or peptide: Chimallin
Function / homologyhost cell cytoplasm / Chimallin
Function and homology information
Biological speciesGoslarvirus / Escherichia phage vB_EcoM_Goslar (virus)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.6 Å
AuthorsLaughlin TG / Deep A / Prichard AM / Seitz C / Gu Y / Enustun E / Suslov S / Khanna K / Birkholz EA / Amaro RE ...Laughlin TG / Deep A / Prichard AM / Seitz C / Gu Y / Enustun E / Suslov S / Khanna K / Birkholz EA / Amaro RE / Pogliano J / Corbett KD / Villa E
Funding support United States, 4 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R01-GM129245 United States
National Science Foundation (NSF, United States)NSF DBI 1920374 United States
Howard Hughes Medical Institute (HHMI) United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R21AI148814 United States
CitationJournal: Nature / Year: 2022
Title: Architecture and self-assembly of the jumbo bacteriophage nuclear shell.
Authors: Thomas G Laughlin / Amar Deep / Amy M Prichard / Christian Seitz / Yajie Gu / Eray Enustun / Sergey Suslov / Kanika Khanna / Erica A Birkholz / Emily Armbruster / J Andrew McCammon / Rommie ...Authors: Thomas G Laughlin / Amar Deep / Amy M Prichard / Christian Seitz / Yajie Gu / Eray Enustun / Sergey Suslov / Kanika Khanna / Erica A Birkholz / Emily Armbruster / J Andrew McCammon / Rommie E Amaro / Joe Pogliano / Kevin D Corbett / Elizabeth Villa /
Abstract: Bacteria encode myriad defences that target the genomes of infecting bacteriophage, including restriction-modification and CRISPR-Cas systems. In response, one family of large bacteriophages uses a ...Bacteria encode myriad defences that target the genomes of infecting bacteriophage, including restriction-modification and CRISPR-Cas systems. In response, one family of large bacteriophages uses a nucleus-like compartment to protect its replicating genomes by excluding host defence factors. However, the principal composition and structure of this compartment remain unknown. Here we find that the bacteriophage nuclear shell assembles primarily from one protein, which we name chimallin (ChmA). Combining cryo-electron tomography of nuclear shells in bacteriophage-infected cells and cryo-electron microscopy of a minimal chimallin compartment in vitro, we show that chimallin self-assembles as a flexible sheet into closed micrometre-scale compartments. The architecture and assembly dynamics of the chimallin shell suggest mechanisms for its nucleation and growth, and its role as a scaffold for phage-encoded factors mediating macromolecular transport, cytoskeletal interactions, and viral maturation.
History
DepositionNov 6, 2021-
Header (metadata) releaseJul 27, 2022-
Map releaseJul 27, 2022-
UpdateAug 24, 2022-
Current statusAug 24, 2022Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_25395.png

Downloads & links

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Map

FileDownload / File: emd_25395.map.gz / Format: CCP4 / Size: 30.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 0.99019 Å
Density
Contour LevelBy AUTHOR: 0.008
Minimum - Maximum-0.017383745 - 0.055120423
Average (Standard dev.)0.00040104345 (±0.002962553)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions200200200
Spacing200200200
CellA=B=C: 198.03801 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_25395_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_25395_half_map_1.map
Projections & Slices
AxesZYX

Projections

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Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_25395_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Goslar chimallin tetramer localized reconstruction

EntireName: Goslar chimallin tetramer localized reconstruction
Components
  • Complex: Goslar chimallin tetramer localized reconstruction
    • Protein or peptide: Chimallin

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Supramolecule #1: Goslar chimallin tetramer localized reconstruction

SupramoleculeName: Goslar chimallin tetramer localized reconstruction / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Goslarvirus
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Macromolecule #1: Chimallin

MacromoleculeName: Chimallin / type: protein_or_peptide / ID: 1 / Number of copies: 12 / Enantiomer: LEVO
Source (natural)Organism: Escherichia phage vB_EcoM_Goslar (virus)
Molecular weightTheoretical: 69.889445 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: SNAMGLDVRN NGNDNVEIRA AETRTAQRAD EALETAADFA GQPKVTHTMR TINRTLSRRI SRNTGSEQVL NLRRLMEKYL EDTRFKDDF IFVAVDPNQY SVPYPTLVVM SGAKVGDHNH FFGYVLPLVA GLAPLPRREE QGPHGNILVP RTWVDNLNGT F INEVMAAM ...String:
SNAMGLDVRN NGNDNVEIRA AETRTAQRAD EALETAADFA GQPKVTHTMR TINRTLSRRI SRNTGSEQVL NLRRLMEKYL EDTRFKDDF IFVAVDPNQY SVPYPTLVVM SGAKVGDHNH FFGYVLPLVA GLAPLPRREE QGPHGNILVP RTWVDNLNGT F INEVMAAM YAAIGGKSNG TARIAGLAVV TNEITAESAH LATTLLSAAD NAIQTAIEIR LGDKLGLPQF NLGMMASDQP IS SVQYNTS GMQDSDIVGN PVRSDITVTI SNRIRQAMSD YDSQQRLVAT TGYIDLTYSP QNPTFNQGPV LVNGYPVPPT VQY QPRYVM TSAYPLELDA FTPNTFVLGL IGTIATLNSG MAWAQSLISN AARGIGPHNP GALAMVLDPE VTAPLDLSTQ TNEQ IYKFL QQVLYPSLLI SIDVPEEGEY SWLLRMIPAA EKIYTGKVEG EVREISEGYK ALYRAFDDVT LGCFSKKYQY GLPLV YATG NRIPLGHYNH QDGHRHDIRD MDDLYMMNIT NPDTVEAWED SFDRTDMTMS QRVVARHEII DRVLSGSWEQ TGWAMR YDF DPLALQALIE AAADAGFTIR PENIQHLAGT AVRGNMAARA RGLGNISGNI YARSDRPNVG VNNMGGAFNL F

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration2.5 mg/mL
BufferpH: 7.5
GridModel: Quantifoil R2/2 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: HOLEY ARRAY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Atmosphere: AIR / Pretreatment - Pressure: 0.019 kPa / Details: 20 mA
VitrificationCryogen name: ETHANE-PROPANE / Chamber humidity: 100 % / Chamber temperature: 289 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm
Specialist opticsEnergy filter - Name: GIF Quantum LS / Energy filter - Slit width: 20 eV
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Digitization - Frames/image: 1-44 / Number grids imaged: 1 / Number real images: 3921 / Average electron dose: 40.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 471192
CTF correctionSoftware: (Name: Warp, RELION)
Startup modelType of model: OTHER / Details: sub-volume of parent map
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION
Final reconstructionApplied symmetry - Point group: C4 (4 fold cyclic) / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 2.6 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: SerialEM / Number images used: 351835
FSC plot (resolution estimation)

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