EMDB-15793: Translating 70S ribosome in the unrotated state (P and E, tRNAs)

Basic information

Entry

Database: EMDB / ID: EMD-15793

• Title: Translating 70S ribosome in the unrotated state (P and E, tRNAs)
• Map data: Post-processed (localfilter from cryoSPARC) map from Homogeneous Refinement

Sample

• Complex: 70S ribosomeRibosome
  • Protein or peptide: x 46 types
  • RNA: x 5 types
• Protein or peptide: x 2 types
• Ligand: x 4 types

• Keywords: 70S / bacterial / translation / high-resolution / RIBOSOME

Function / homology

Function:

mRNA base-pairing translational repressor activity / ornithine decarboxylase inhibitor activity / misfolded RNA binding / transcription antitermination factor activity, RNA binding / Group I intron splicing / RNA folding / transcriptional attenuation / endoribonuclease inhibitor activity / RNA-binding transcription regulator activity / positive regulation of ribosome biogenesis / negative regulation of cytoplasmic translation / DnaA-L2 complex / four-way junction DNA binding / negative regulation of translational initiation / translation repressor activity / negative regulation of DNA-templated DNA replication initiation / regulation of mRNA stability / ribosome assembly / mRNA regulatory element binding translation repressor activity / response to reactive oxygen species / assembly of large subunit precursor of preribosome / positive regulation of RNA splicing / DNA endonuclease activity / transcription elongation factor complex / cytosolic ribosome assembly / regulation of DNA-templated transcription elongation / transcription antitermination / regulation of cell growth / maintenance of translational fidelity / DNA-templated transcription termination / response to radiation / mRNA 5'-UTR binding / ribosomal small subunit biogenesis / ribosomal large subunit assembly / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / ribosome binding / large ribosomal subunit / ribosome biogenesis / regulation of translation / cytoplasmic translation / small ribosomal subunit / 5S rRNA binding / cytosolic large ribosomal subunit / transferase activity / tRNA binding / negative regulation of translation / rRNA binding / molecular adaptor activity / ribosome / structural constituent of ribosome / translation / response to antibiotic / mRNA binding / negative regulation of DNA-templated transcription / DNA binding / RNA binding / zinc ion binding / membrane / cytosol / cytoplasm

Domain/homology:

Ribosomal protein S21, conserved site / Ribosomal protein S21 signature. / Ribosomal protein L25, short-form / Ribosomal protein S14, bacterial/plastid / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S16, conserved site / Ribosomal protein S16 signature. / Ribosomal protein S21 / Ribosomal protein L21, conserved site / Ribosomal protein L21 signature. / Ribosomal protein L16 signature 1. / : / Ribosomal protein L6, conserved site / Ribosomal protein L6 signature 1. / Ribosomal protein L16, conserved site / Ribosomal protein L16 signature 2. / Ribosomal protein L17 signature. / Ribosomal protein L9 signature. / Ribosomal protein L9, bacteria/chloroplast / Ribosomal protein L9, C-terminal / Ribosomal protein L9, C-terminal domain / Ribosomal protein L9, C-terminal domain superfamily / Ribosomal L25p family / Ribosomal protein L25 / Ribosomal protein L28/L24 superfamily / Ribosomal protein L36 signature. / Ribosomal protein L25/Gln-tRNA synthetase, N-terminal / Ribosomal protein L32p, bacterial type / Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain superfamily / Ribosomal protein L9, N-terminal domain superfamily / Ribosomal protein L9 / Ribosomal protein L9, N-terminal / Ribosomal protein L9, N-terminal domain / Ribosomal protein L28 / Ribosomal protein L35, conserved site / Ribosomal protein L35 signature. / Ribosomal protein L33, conserved site / Ribosomal protein L33 signature. / Ribosomal protein L35, non-mitochondrial / Ribosomal protein L5, bacterial-type / Ribosomal protein L6, bacterial-type / Ribosomal protein L18, bacterial-type / Ribosomal protein L19, conserved site / Ribosomal protein L19 signature. / Ribosomal protein L36 / Ribosomal protein L36 superfamily / Ribosomal protein L36 / Ribosomal protein L9/RNase H1, N-terminal / Ribosomal protein L20 signature. / Ribosomal protein S3, bacterial-type / Ribosomal protein S6, conserved site / Ribosomal protein S6 signature. / Ribosomal protein L27, conserved site / Ribosomal protein L27 signature. / Ribosomal protein S19, bacterial-type / Ribosomal protein S7, bacterial/organellar-type / Ribosomal protein S11, bacterial-type / Ribosomal protein S13, bacterial-type / Ribosomal protein S20 / Ribosomal protein S20 superfamily / Ribosomal protein S20 / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S4, bacterial-type / Ribosomal protein L14P, bacterial-type / Ribosomal protein L34, conserved site / Ribosomal protein L34 signature. / 30S ribosomal protein S17 / Ribosomal protein S5, bacterial-type / Ribosomal protein L22, bacterial/chloroplast-type / Ribosomal protein S6, plastid/chloroplast / Ribosomal protein L35 / Ribosomal protein L35 superfamily / Ribosomal protein L2, bacterial/organellar-type / Ribosomal protein L35 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal L28 family / Ribosomal protein L33 / Ribosomal protein L33 / Ribosomal protein L28/L24 / Ribosomal protein L33 superfamily / : / Ribosomal protein L30, bacterial-type / Ribosomal protein L16 / Ribosomal protein L18 / Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / L28p-like / Ribosomal protein L20 / Ribosomal protein S16 / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein L20 / Ribosomal protein L20, C-terminal / Ribosomal protein L21 / Ribosomal protein L27 / Ribosomal L27 protein / Ribosomal protein L19 / Ribosomal protein L19 superfamily

Component:

Small ribosomal subunit protein bS6 / Small ribosomal subunit protein uS7 / Large ribosomal subunit protein uL15 / Large ribosomal subunit protein bL19 / Large ribosomal subunit protein bL20 / Large ribosomal subunit protein bL27 / Large ribosomal subunit protein bL28 / Large ribosomal subunit protein uL29 / Large ribosomal subunit protein bL32 / Large ribosomal subunit protein bL33 / Large ribosomal subunit protein bL34 / Large ribosomal subunit protein bL35 / Large ribosomal subunit protein bL36A / Large ribosomal subunit protein bL9 / Small ribosomal subunit protein uS10 / Small ribosomal subunit protein uS11 / Small ribosomal subunit protein uS12 / Small ribosomal subunit protein uS13 / Small ribosomal subunit protein bS16 / Small ribosomal subunit protein bS18 / Small ribosomal subunit protein uS19 / Small ribosomal subunit protein bS20 / Small ribosomal subunit protein uS2 / Small ribosomal subunit protein uS3 / Small ribosomal subunit protein uS4 / Small ribosomal subunit protein uS5 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein uL13 / Large ribosomal subunit protein uL14 / Large ribosomal subunit protein uL16 / Large ribosomal subunit protein uL23 / Small ribosomal subunit protein uS15 / Large ribosomal subunit protein bL17 / Large ribosomal subunit protein bL21 / Large ribosomal subunit protein uL30 / Large ribosomal subunit protein uL6 / Small ribosomal subunit protein uS14 / Small ribosomal subunit protein uS17 / Large ribosomal subunit protein uL18 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein uL24 / Large ribosomal subunit protein uL4 / Large ribosomal subunit protein uL22 / Large ribosomal subunit protein uL5 / Small ribosomal subunit protein bS21 / Large ribosomal subunit protein bL25

• Biological species: Escherichia coli B (bacteria)
• Method: single particle reconstruction / cryo EM / Resolution: 1.55 Å
• Authors: Fromm SA / O'Connor KM / Purdy M / Bhatt PR / Loughran G / Atkins JF / Jomaa A / Mattei S

Funding support

Ireland, European Union, 4 items

Organization	Grant number	Country
Other government
Other government
Irish Research Council	IRCLA/2019/74	Ireland
iNEXT-Discovery	871037	European Union

Citation

Journal: Nat Commun / Year: 2023
Title: The translating bacterial ribosome at 1.55 Å resolution generated by cryo-EM imaging services.
Authors: Simon A Fromm / Kate M O'Connor / Michael Purdy / Pramod R Bhatt / Gary Loughran / John F Atkins / Ahmad Jomaa / Simone Mattei /
Abstract: Our understanding of protein synthesis has been conceptualised around the structure and function of the bacterial ribosome. This complex macromolecular machine is the target of important antimicrobial drugs, an integral line of defence against infectious diseases. Here, we describe how open access to cryo-electron microscopy facilities combined with bespoke user support enabled structural determination of the translating ribosome from Escherichia coli at 1.55 Å resolution. The obtained structures allow for direct determination of the rRNA sequence to identify ribosome polymorphism sites in the E. coli strain used in this study and enable interpretation of the ribosomal active and peripheral sites at unprecedented resolution. This includes scarcely populated chimeric hybrid states of the ribosome engaged in several tRNA translocation steps resolved at ~2 Å resolution. The current map not only improves our understanding of protein synthesis but also allows for more precise structure-based drug design of antibiotics to tackle rising bacterial resistance.

#1: Journal: Biorxiv / Year: 2022
Title: The translating bacterial ribosome at 1.55 angstrom resolution by open access cryo-EM
Authors: Fromm SA / O'Connor KM / Purdy M / Bhatt PR / Loughran G / Atkins JF / Jomaa A / Mattei S

History

Deposition	Sep 8, 2022	-
Header (metadata) release	Nov 30, 2022	-
Map release	Nov 30, 2022	-
Update	Apr 24, 2024	-
Current status	Apr 24, 2024	Processing site: PDBe / Status: Released

Map

• File: Download / File: emd_15793.map.gz / Format: CCP4 / Size: 669.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Post-processed (localfilter from cryoSPARC) map from Homogeneous Refinement
• Voxel size: X=Y=Z: 0.731 Å

Density

Contour Level	By AUTHOR: 0.2
Minimum - Maximum	-2.4977798 - 7.947491
Average (Standard dev.)	0.007840831 (±0.09315578)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 560 560 560 Spacing 560 560 560
Cell	A=B=C: 409.36002 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_15793_msk_1.map

Mask #2

• File: emd_15793_msk_2.map

Half map: half map A

• File: emd_15793_half_map_1.map
• Annotation: half map A

Half map: half map B

• File: emd_15793_half_map_2.map
• Annotation: half map B

Sample components

Entire : 70S ribosome

• Entire: Name: 70S ribosomeRibosome

Components

• Complex: 70S ribosomeRibosome
  • Protein or peptide: 50S ribosomal protein L33
  • Protein or peptide: 50S ribosomal protein L34
  • Protein or peptide: 50S ribosomal protein L35
  • Protein or peptide: 50S ribosomal protein L36
  • RNA: 16S rRNA
  • Protein or peptide: 30S ribosomal protein S2
  • Protein or peptide: 30S ribosomal protein S3
  • Protein or peptide: 30S ribosomal protein S4
  • Protein or peptide: 30S ribosomal protein S5
  • Protein or peptide: 30S ribosomal protein S6
  • Protein or peptide: 30S ribosomal protein S7
  • Protein or peptide: 30S ribosomal protein S8
  • Protein or peptide: 30S ribosomal protein S9
  • Protein or peptide: 30S ribosomal protein S10
  • Protein or peptide: 30S ribosomal protein S12
  • Protein or peptide: 30S ribosomal protein S13
  • Protein or peptide: 30S ribosomal protein S14
  • Protein or peptide: 30S ribosomal protein S15
  • Protein or peptide: 30S ribosomal protein S16
  • Protein or peptide: 30S ribosomal protein S17
  • Protein or peptide: 30S ribosomal protein S18
  • Protein or peptide: 30S ribosomal protein S19
  • Protein or peptide: 30S ribosomal protein S20
  • Protein or peptide: 30S ribosomal protein S21
  • RNA: mRNAMessenger RNA
  • RNA: P-site tRNA
  • RNA: 23S rRNA23S ribosomal RNA
  • RNA: 5S rRNA5S ribosomal RNA
  • Protein or peptide: 50S ribosomal protein L2
  • Protein or peptide: 50S ribosomal protein L3
  • Protein or peptide: 50S ribosomal protein L4
  • Protein or peptide: 50S ribosomal protein L5
  • Protein or peptide: 50S ribosomal protein L6
  • Protein or peptide: 50S ribosomal protein L9
  • Protein or peptide: 50S ribosomal protein L13
  • Protein or peptide: 50S ribosomal protein L14
  • Protein or peptide: 50S ribosomal protein L15
  • Protein or peptide: 50S ribosomal protein L17
  • Protein or peptide: 50S ribosomal protein L18
  • Protein or peptide: 50S ribosomal protein L19
  • Protein or peptide: 50S ribosomal protein L20
  • Protein or peptide: 50S ribosomal protein L21
  • Protein or peptide: 50S ribosomal protein L22
  • Protein or peptide: 50S ribosomal protein L23
  • Protein or peptide: 50S ribosomal protein L24
  • Protein or peptide: 50S ribosomal protein L25
  • Protein or peptide: 50S ribosomal protein L27
  • Protein or peptide: 50S ribosomal protein L28
  • Protein or peptide: 50S ribosomal protein L29
  • Protein or peptide: 50S ribosomal protein L30
  • Protein or peptide: 50S ribosomal protein L32
• Protein or peptide: 30S ribosomal protein S11
• Protein or peptide: 50S ribosomal protein L16
• Ligand: ZINC ION
• Ligand: POTASSIUM IONPotassium
• Ligand: MAGNESIUM ION
• Ligand: water

Supramolecule #1: 70S ribosome

• Supramolecule: Name: 70S ribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#14, #16-#38, #40-#53 / Details: translating with P-site tRNA and mRNA
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 2.5 MDa

Macromolecule #1: 50S ribosomal protein L33

• Macromolecule: Name: 50S ribosomal protein L33 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 6.388631 KDa

Sequence

String:

MAKGIREKIK LVSSAGTGHF YTTTKNKRTK PEKLELKKFD PVVRQHVIYK EAKIK

UniProtKB: Large ribosomal subunit protein bL33

Macromolecule #2: 50S ribosomal protein L34

• Macromolecule: Name: 50S ribosomal protein L34 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 5.397463 KDa

Sequence

String:

MKRTFQPSVL KRNRSHGFRA RMATKNGRQV LARRRAKGRA RLTVSK

UniProtKB: Large ribosomal subunit protein bL34

Macromolecule #3: 50S ribosomal protein L35

• Macromolecule: Name: 50S ribosomal protein L35 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 7.313032 KDa

Sequence

String:

MPKIKTVRGA AKRFKKTGKG GFKHKHANLR HILTKKATKR KRHLRPKAMV SKGDLGLVIA CLPYA

UniProtKB: Large ribosomal subunit protein bL35

Macromolecule #4: 50S ribosomal protein L36

• Macromolecule: Name: 50S ribosomal protein L36 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 4.37739 KDa

Sequence

String:

MKVRASVKKL CRNCKIVKRD GVIRVICSAE PKHKQRQG

UniProtKB: Large ribosomal subunit protein bL36A

Macromolecule #6: 30S ribosomal protein S2

• Macromolecule: Name: 30S ribosomal protein S2 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 26.78167 KDa

Sequence

String:

MATVSMRDML KAGVHFGHQT RYWNPKMKPF IFGARNKVHI INLEKTVPMF NEALAELNKI ASRKGKILFV GTKRAASEAV KDAALSCDQ FFVNHRWLGG MLTNWKTVRQ SIKRLKDLET QSQDGTFDKL TKKEALMRTR ELEKLENSLG GIKDMGGLPD A LFVIDADH EHIAIKEANN LGIPVFAIVD TNSDPDGVDF VIPGNDDAIR AVTLYLGAVA ATVREGRSQD LASQAEESFV EA E

UniProtKB: Small ribosomal subunit protein uS2

Macromolecule #7: 30S ribosomal protein S3

• Macromolecule: Name: 30S ribosomal protein S3 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 26.031316 KDa

Sequence

String:

MGQKVHPNGI RLGIVKPWNS TWFANTKEFA DNLDSDFKVR QYLTKELAKA SVSRIVIERP AKSIRVTIHT ARPGIVIGKK GEDVEKLRK VVADIAGVPA QINIAEVRKP ELDAKLVADS ITSQLERRVM FRRAMKRAVQ NAMRLGAKGI KVEVSGRLGG A EIARTEWY REGRVPLHTL RADIDYNTSE AHTTYGVIGV KVWIFKGEIL GGMAAVEQPE KPAAQPKKQQ RKGRK

UniProtKB: Small ribosomal subunit protein uS3

Macromolecule #8: 30S ribosomal protein S4

• Macromolecule: Name: 30S ribosomal protein S4 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 23.514199 KDa

Sequence

String:

MARYLGPKLK LSRREGTDLF LKSGVRAIDT KCKIEQAPGQ HGARKPRLSD YGVQLREKQK VRRIYGVLER QFRNYYKEAA RLKGNTGEN LLALLEGRLD NVVYRMGFGA TRAEARQLVS HKAIMVNGRV VNIASYQVSP NDVVSIREKA KKQSRVKAAL E LAEQREKP TWLEVDAGKM EGTFKRKPER SDLSADINEH LIVELYSK

UniProtKB: Small ribosomal subunit protein uS4

Macromolecule #9: 30S ribosomal protein S5

• Macromolecule: Name: 30S ribosomal protein S5 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 17.629398 KDa

Sequence

String:

MAHIEKQAGE LQEKLIAVNR VSKTVKGGRI FSFTALTVVG DGNGRVGFGY GKAREVPAAI QKAMEKARRN MINVALNNGT LQHPVKGVH TGSRVFMQPA SEGTGIIAGG AMRAVLEVAG VHNVLAKAYG STNPINVVRA TIDGLENMNS PEMVAAKRGK S VEEILGK

UniProtKB: Small ribosomal subunit protein uS5

Macromolecule #10: 30S ribosomal protein S6

• Macromolecule: Name: 30S ribosomal protein S6 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 15.727512 KDa

Sequence

String:

MRHYEIVFMV HPDQSEQVPG MIERYTAAIT GAEGKIHRLE DWGRRQLAYP INKLHKAHYV LMNVEAPQEV IDELETTFRF NDAVIRSMV MRTKHAVTEA SPMVKAKDER RERRDDFANE TADDAEAGDS EEEEEE

UniProtKB: Small ribosomal subunit protein bS6

Macromolecule #11: 30S ribosomal protein S7

• Macromolecule: Name: 30S ribosomal protein S7 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 20.055156 KDa

Sequence

String:

MPRRRVIGQR KILPDPKFGS ELLAKFVNIL MVDGKKSTAE SIVYSALETL AQRSGKSELE AFEVALENVR PTVEVKSRRV GGSTYQVPV EVRPVRRNAL AMRWIVEAAR KRGDKSMALR LANELSDAAE NKGTAVKKRE DVHRMAEANK AFAHYRWLSL R SFSHQAGA SSKQPALGYL N

UniProtKB: Small ribosomal subunit protein uS7

Macromolecule #12: 30S ribosomal protein S8

• Macromolecule: Name: 30S ribosomal protein S8 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 14.146557 KDa

Sequence

String:

MSMQDPIADM LTRIRNGQAA NKAAVTMPSS KLKVAIANVL KEEGFIEDFK VEGDTKPELE LTLKYFQGKA VVESIQRVSR PGLRIYKRK DELPKVMAGL GIAVVSTSKG VMTDRAARQA GLGGEIICYV A

UniProtKB: Small ribosomal subunit protein uS8

Macromolecule #13: 30S ribosomal protein S9

• Macromolecule: Name: 30S ribosomal protein S9 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 14.88627 KDa

Sequence

String:

MAENQYYGTG RRKSSAARVF IKPGNGKIVI NQRSLEQYFG RETARMVVRQ PLELVDMVEK LDLYITVKGG GISGQAGAIR HGITRALME YDESLRSELR KAGFVTRDAR QVERKKVGLR KARRRPQFSK R

UniProtKB: Small ribosomal subunit protein uS9

Macromolecule #14: 30S ribosomal protein S10

• Macromolecule: Name: 30S ribosomal protein S10 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 11.755597 KDa

Sequence

String:

MQNQRIRIRL KAFDHRLIDQ ATAEIVETAK RTGAQVRGPI PLPTRKERFT VLISPHVNKD ARDQYEIRTH LRLVDIVEPT EKTVDALMR LDLAAGVDVQ ISLG

UniProtKB: Small ribosomal subunit protein uS10

Macromolecule #15: 30S ribosomal protein S11

• Macromolecule: Name: 30S ribosomal protein S11 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 13.871959 KDa

Sequence

String:

MAKAPIRARK RVRKQVSDGV AHIHASFNNT IVTITDRQGN ALGWATAGGS GFRGSRKSTP FAAQVAAERC ADAVKEYGIK NLEVMVKGP GPGRESTIRA LNAAGFRITN ITDVTPIPH(IAS) GCRPPKKRRV

UniProtKB: Small ribosomal subunit protein uS11

Macromolecule #16: 30S ribosomal protein S12

• Macromolecule: Name: 30S ribosomal protein S12 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 13.814249 KDa

Sequence

String:

MATVNQLVRK PRARKVAKSN VPALEACPQK RGVCTRVYTT TPKKPNSALR KVCRVRLTNG FEVTSYIGGE GHNLQEHSVI LIRGGRVK(D2T) LPGVRYHTVR GALDCSGVKD RKQARSKYGV KRPKA

UniProtKB: Small ribosomal subunit protein uS12

Macromolecule #17: 30S ribosomal protein S13

• Macromolecule: Name: 30S ribosomal protein S13 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 13.128467 KDa

Sequence

String:

MARIAGINIP DHKHAVIALT SIYGVGKTRS KAILAAAGIA EDVKISELSE GQIDTLRDEV AKFVVEGDLR REISMSIKRL MDLGCYRGL RHRRGLPVRG QRTKTNARTR KGPRKPIKK

UniProtKB: Small ribosomal subunit protein uS13

Macromolecule #18: 30S ribosomal protein S14

• Macromolecule: Name: 30S ribosomal protein S14 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 11.60656 KDa

Sequence

String:

MAKQSMKARE VKRVALADKY FAKRAELKAI ISDVNASDED RWNAVLKLQT LPRDSSPSRQ RNRCRQTGRP HGFLRKFGLS RIKVREAAM RGEIPGLKKA SW

UniProtKB: Small ribosomal subunit protein uS14

Macromolecule #19: 30S ribosomal protein S15

• Macromolecule: Name: 30S ribosomal protein S15 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 10.290816 KDa

Sequence

String:

MSLSTEATAK IVSEFGRDAN DTGSTEVQVA LLTAQINHLQ GHFAEHKKDH HSRRGLLRMV SQRRKLLDYL KRKDVARYTQ LIERLGLRR

UniProtKB: Small ribosomal subunit protein uS15

Macromolecule #20: 30S ribosomal protein S16

• Macromolecule: Name: 30S ribosomal protein S16 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 9.207572 KDa

Sequence

String:

MVTIRLARHG AKKRPFYQVV VADSRNARNG RFIERVGFFN PIASEKEEGT RLDLDRIAHW VGQGATISDR VAALIKEVNK AA

UniProtKB: Small ribosomal subunit protein bS16

Macromolecule #21: 30S ribosomal protein S17

• Macromolecule: Name: 30S ribosomal protein S17 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 9.724491 KDa

Sequence

String:

MTDKIRTLQG RVVSDKMEKS IVVAIERFVK HPIYGKFIKR TTKLHVHDEN NECGIGDVVE IRECRPLSKT KSWTLVRVVE KAVL

UniProtKB: Small ribosomal subunit protein uS17

Macromolecule #22: 30S ribosomal protein S18

• Macromolecule: Name: 30S ribosomal protein S18 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 9.005472 KDa

Sequence

String:

MARYFRRRKF CRFTAEGVQE IDYKDIATLK NYITESGKIV PSRITGTRAK YQRQLARAIK RARYLSLLPY TDRHQ

UniProtKB: Small ribosomal subunit protein bS18

Macromolecule #23: 30S ribosomal protein S19

• Macromolecule: Name: 30S ribosomal protein S19 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 10.455355 KDa

Sequence

String:

MPRSLKKGPF IDLHLLKKVE KAVESGDKKP LRTWSRRSTI FPNMIGLTIA VHNGRQHVPV FVTDEMVGHK LGEFAPTRTY RGHAADKKA KKK

UniProtKB: Small ribosomal subunit protein uS19

Macromolecule #24: 30S ribosomal protein S20

• Macromolecule: Name: 30S ribosomal protein S20 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 9.708464 KDa

Sequence

String:

MANIKSAKKR AIQSEKARKH NASRRSMMRT FIKKVYAAIE AGDKAAAQKA FNEMQPIVDR QAAKGLIHKN KAARHKANLT AQINKLA

UniProtKB: Small ribosomal subunit protein bS20

Macromolecule #25: 30S ribosomal protein S21

• Macromolecule: Name: 30S ribosomal protein S21 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 8.524039 KDa

Sequence

String:

MPVIKVRENE PFDVALRRFK RSCEKAGVLA EVRRREFYEK PTTERKRAKA SAVKRHAKKL ARENARRTRL Y

UniProtKB: Small ribosomal subunit protein bS21

Macromolecule #30: 50S ribosomal protein L2

• Macromolecule: Name: 50S ribosomal protein L2 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 29.923619 KDa

Sequence

String:

MAVVKCKPTS PGRRHVVKVV NPELHKGKPF APLLEKNSKS GGRNNNGRIT TRHIGGGHKQ AYRIVDFKRN KDGIPAVVER LEYDPNRSA NIALVLYKDG ERRYILAPKG LKAGDQIQSG VDAAIKPGNT LPMRNIPVGS TVHNVEMKPG KGGQLARSAG T YVQIVARD GAYVTLRLRS GEMRKVEADC RATLGEVGNA EHMLRVLGKA GAARWRGVRP TVRGTAMNPV DHPHGGGEGR NF GKHPVTP WGVQTKGKKT RSNKRTDKFI VRRRSK

UniProtKB: Large ribosomal subunit protein uL2

Macromolecule #31: 50S ribosomal protein L3

• Macromolecule: Name: 50S ribosomal protein L3 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 22.291562 KDa

Sequence

String:

MIGLVGKKVG MTRIFTEDGV SIPVTVIEVE ANRVTQVKDL ANDGYRAIQV TTGAKKANRV TKPEAGHFAK AGVEAGRGLW EFRLAEGEE FTVGQSISVE LFADVKKVDV TGTSKGKGFA GTVKRWNFRT QDATHGNSLS HRVPGSIGQN (MEQ)TPGKVF KG KKMAGQMGNE RVTVQSLDVV RVDAERNLLL VKGAVPGATG SDLIVKPAVK A

UniProtKB: Large ribosomal subunit protein uL3

Macromolecule #32: 50S ribosomal protein L4

• Macromolecule: Name: 50S ribosomal protein L4 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 22.121566 KDa

Sequence

String:

MELVLKDAQS ALTVSETTFG RDFNEALVHQ VVVAYAAGAR QGTRAQKTRA EVTGSGKKPW RQKGTGRARS GSIKSPIWRS GGVTFAARP QDHSQKVNKK MYRGALKSIL SELVRQDRLI VVEKFSVEAP KTKLLAQKLK DMALEDVLII TGELDENLFL A ARNLHKVD VRDATGIDPV SLIAFDKVVM TADAVKQVEE MLA

UniProtKB: Large ribosomal subunit protein uL4

Macromolecule #33: 50S ribosomal protein L5

• Macromolecule: Name: 50S ribosomal protein L5 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 20.333611 KDa

Sequence

String:

MAKLHDYYKD EVVKKLMTEF NYNSVMQVPR VEKITLNMGV GEAIADKKLL DNAAADLAAI SGQKPLITKA RKSVAGFKIR QGYPIGCKV TLRGERMWEF FERLITIAVP RIRDFRGLSA KSFDGRGNYS MGVREQIIFP EIDYDKVDRV RGLDITITTT A KSDEEGRA LLAAFDFPFR K

UniProtKB: Large ribosomal subunit protein uL5

Macromolecule #34: 50S ribosomal protein L6

• Macromolecule: Name: 50S ribosomal protein L6 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 18.932791 KDa

Sequence

String:

MSRVAKAPVV VPAGVDVKIN GQVITIKGKN GELTRTLNDA VEVKHADNTL TFGPRDGYAD GWAQAGTARA LLNSMVIGVT EGFTKKLQL VGVGYRAAVK GNVINLSLGF SHPVDHQLPA GITAECPTQT EIVLKGADKQ VIGQVAADLR AYRRPEPYKG K GVRYADEV VRTKEAKKK

UniProtKB: Large ribosomal subunit protein uL6

Macromolecule #35: 50S ribosomal protein L9

• Macromolecule: Name: 50S ribosomal protein L9 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 15.78902 KDa

Sequence

String:

MQVILLDKVA NLGSLGDQVN VKAGYARNFL VPQGKAVPAT KKNIEFFEAR RAELEAKLAE VLAAANARAE KINALETVTI ASKAGDEGK LFGSIGTRDI ADAVTAAGVE VAKSEVRLPN GVLRTTGEHE VSFQVHSEVF AKVIVNVVAE

UniProtKB: Large ribosomal subunit protein bL9

Macromolecule #36: 50S ribosomal protein L13

• Macromolecule: Name: 50S ribosomal protein L13 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 16.050606 KDa

Sequence

String:

MKTFTAKPET VKRDWYVVDA TGKTLGRLAT ELARRLRGKH KAEYTPHVDT GDYIIVLNAD KVAVTGNKRT DKVYYHHTGH IGGIKQATF EEMIARRPER VIEIAVKGML PKGPLGRAMF RKLKVYAGNE HNHAAQQPQV LDI

UniProtKB: Large ribosomal subunit protein uL13

Macromolecule #37: 50S ribosomal protein L14

• Macromolecule: Name: 50S ribosomal protein L14 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 13.565067 KDa

Sequence

String:

MIQEQTMLNV ADNSGARRVM CIKVLGGSHR RYAGVGDIIK ITIKEAIPRG KVKKGDVLKA VVVRTKKGVR RPDGSVIRFD GNACVLLNN NSEQPIGTRI FGPVTRELRS EKFMKIISLA PEVL

UniProtKB: Large ribosomal subunit protein uL14

Macromolecule #38: 50S ribosomal protein L15

• Macromolecule: Name: 50S ribosomal protein L15 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 15.008471 KDa

Sequence

String:

MRLNTLSPAE GSKKAGKRLG RGIGSGLGKT GGRGHKGQKS RSGGGVRRGF EGGQMPLYRR LPKFGFTSRK AAITAEIRLS DLAKVEGGV VDLNTLKAAN IIGIQIEFAK VILAGEVTTP VTVRGLRVTK GARAAIEAAG GKIEE

UniProtKB: Large ribosomal subunit protein uL15

Macromolecule #39: 50S ribosomal protein L16

• Macromolecule: Name: 50S ribosomal protein L16 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 15.329343 KDa

Sequence

String:

MLQPKRTKFR KMHKGRNRGL AQGTDVSFGS FGLKAVGRGR LTARQIEAAR RAMTRAVKRQ GKIWIRVFPD KPITEKPLAV (4D4)(MS6)GKGKGNV EYWVALIQPG KVLYEMDGVP EELAREAFKL AAAKLPIKTT FVTKTVM

UniProtKB: Large ribosomal subunit protein uL16

Macromolecule #40: 50S ribosomal protein L17

• Macromolecule: Name: 50S ribosomal protein L17 / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 14.393657 KDa

Sequence

String:

MRHRKSGRQL NRNSSHRQAM FRNMAGSLVR HEIIKTTLPK AKELRRVVEP LITLAKTDSV ANRRLAFART RDNEIVAKLF NELGPRFAS RAGGYTRILK CGFRAGDNAP MAYIELVDRS EKAEAAAE

UniProtKB: Large ribosomal subunit protein bL17

Macromolecule #41: 50S ribosomal protein L18

• Macromolecule: Name: 50S ribosomal protein L18 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 12.794668 KDa

Sequence

String:

MDKKSARIRR ATRARRKLQE LGATRLVVHR TPRHIYAQVI APNGSEVLVA ASTVEKAIAE QLKYTGNKDA AAAVGKAVAE RALEKGIKD VSFDRSGFQY HGRVQALADA AREAGLQF

UniProtKB: Large ribosomal subunit protein uL18

Macromolecule #42: 50S ribosomal protein L19

• Macromolecule: Name: 50S ribosomal protein L19 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 13.159278 KDa

Sequence

String:

MSNIIKQLEQ EQMKQDVPSF RPGDTVEVKV WVVEGSKKRL QAFEGVVIAI RNRGLHSAFT VRKISNGEGV ERVFQTHSPV VDSISVKRR GAVRKAKLYY LRERTGKAAR IKERLN

UniProtKB: Large ribosomal subunit protein bL19

Macromolecule #43: 50S ribosomal protein L20

• Macromolecule: Name: 50S ribosomal protein L20 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 13.528024 KDa

Sequence

String:

MARVKRGVIA RARHKKILKQ AKGYYGARSR VYRVAFQAVI KAGQYAYRDR RQRKRQFRQL WIARINAAAR QNGISYSKFI NGLKKASVE IDRKILADIA VFDKVAFTAL VEKAKAALA

UniProtKB: Large ribosomal subunit protein bL20

Macromolecule #44: 50S ribosomal protein L21

• Macromolecule: Name: 50S ribosomal protein L21 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 11.586374 KDa

Sequence

String:

MYAVFQSGGK QHRVSEGQTV RLEKLDIATG ETVEFAEVLM IANGEEVKIG VPFVDGGVIK AEVVAHGRGE KVKIVKFRRR KHYRKQQGH RQWFTDVKIT GISA

UniProtKB: Large ribosomal subunit protein bL21

Macromolecule #45: 50S ribosomal protein L22

• Macromolecule: Name: 50S ribosomal protein L22 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 12.253359 KDa

Sequence

String:

METIAKHRHA RSSAQKVRLV ADLIRGKKVS QALDILTYTN KKAAVLVKKV LESAIANAEH NDGADIDDLK VTKIFVDEGP SMKRIMPRA KGRADRILKR TSHITVVVSD R

UniProtKB: Large ribosomal subunit protein uL22

Macromolecule #46: 50S ribosomal protein L23

• Macromolecule: Name: 50S ribosomal protein L23 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 11.22216 KDa

Sequence

String:

MIREERLLKV LRAPHVSEKA STAMEKSNTI VLKVAKDATK AEIKAAVQKL FEVEVEVVNT LVVKGKVKRH GQRIGRRSDW KKAYVTLKE GQNLDFVGGA E

UniProtKB: Large ribosomal subunit protein uL23

Macromolecule #47: 50S ribosomal protein L24

• Macromolecule: Name: 50S ribosomal protein L24 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 11.33925 KDa

Sequence

String:

MAAKIRRDDE VIVLTGKDKG KRGKVKNVLS SGKVIVEGIN LVKKHQKPVP ALNQPGGIVE KEAAIQVSNV AIFNAATGKA DRVGFRFED GKKVRFFKSN SETIK

UniProtKB: Large ribosomal subunit protein uL24

Macromolecule #48: 50S ribosomal protein L25

• Macromolecule: Name: 50S ribosomal protein L25 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 10.713465 KDa

Sequence

String:

MFTINAEVRK EQGKGASRRL RAANKFPAII YGGKEAPLAI ELDHDKVMNM QAKAEFYSEV LTIVVDGKEI KVKAQDVQRH PYKPKLQHI DFVRA

UniProtKB: Large ribosomal subunit protein bL25

Macromolecule #49: 50S ribosomal protein L27

• Macromolecule: Name: 50S ribosomal protein L27 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 9.14654 KDa

Sequence

String:

MAHKKAGGST RNGRDSEAKR LGVKRFGGES VLAGSIIVRQ RGTKFHAGAN VGCGRDHTLF AKADGKVKFE VKGPKNRKFI SIEAE

UniProtKB: Large ribosomal subunit protein bL27

Macromolecule #50: 50S ribosomal protein L28

• Macromolecule: Name: 50S ribosomal protein L28 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 9.027551 KDa

Sequence

String:

MSRVCQVTGK RPVTGNNRSH ALNATKRRFL PNLHSHRFWV ESEKRFVTLR VSAKGMRVID KKGIDTVLAE LRARGEKY

UniProtKB: Large ribosomal subunit protein bL28

Macromolecule #51: 50S ribosomal protein L29

• Macromolecule: Name: 50S ribosomal protein L29 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 7.286464 KDa

Sequence

String:

MKAKELREKS VEELNTELLN LLREQFNLRM QAASGQLQQS HLLKQVRRDV ARVKTLLNEK AGA

UniProtKB: Large ribosomal subunit protein uL29

Macromolecule #52: 50S ribosomal protein L30

• Macromolecule: Name: 50S ribosomal protein L30 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 6.55482 KDa

Sequence

String:

MAKTIKITQT RSAIGRLPKH KATLLGLGLR RIGHTVERED TPAIRGMINA VSFMVKVEE

UniProtKB: Large ribosomal subunit protein uL30

Macromolecule #53: 50S ribosomal protein L32

• Macromolecule: Name: 50S ribosomal protein L32 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Escherichia coli B (bacteria) / Strain: K12
• Molecular weight: Theoretical: 6.463445 KDa

Sequence

String:

MAVQQNKPTR SKRGMRRSHD ALTAVTSLSV DKTSGEKHLR HHITADGYYR GRKVIAK

UniProtKB: Large ribosomal subunit protein bL32

Macromolecule #5: 16S rRNA

• Macromolecule: Name: 16S rRNA / type: rna / ID: 5 / Number of copies: 1
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 503.775938 KDa

Sequence

String:

AAAUUGAAGA GUUUGAUCAU GGCUCAGAUU GAACGCUGGC GGCAGGCCUA ACACAUGCAA GUCGAACGGU AACAGGAAGC AGCUUGCUG CUUCGCUGAC GAGUGGCGGA CGGGUGAGUA AUGUCUGGGA AACUGCCUGA UGGAGGGGGA UAACUACUGG A AACGGUAG CUAAUACCGC AUAACGUCGC AAGACCAAAG AGGGGGACCU UAGGGCCUCU UGCCAUCGGA UGUGCCCAGA UG GGAUUAG CUAGUAGGUG GGGUAACGGC UCACCUAGGC GACGAUCCCU AGCUGGUCUG AGAGGAUGAC CAGCCACACU GGA ACUGAG ACACGGUCCA GACUCCUACG GGAGGCAGCA GUGGGGAAUA UUGCACAAUG GGCGCAAGCC UGAUGCAGCC AUGC CGCGU GUAUGAAGAA GGCCUUCGGG UUGUAAAGUA CUUUCAGCGG GGAGGAAGGG AGUAAAGUUA AUACCUUUGC UCAUU GACG UUACCCGCAG AAGAAGCACC GGCUAACUCC G(PSU)GCCAGCAG CC(G7M)CGGUAAU ACGGAGGGUG CAAGCGUU A AUCGGAAUUA CUGGGCGUAA AGCGCACGCA GGCGGUUUGU UAAGUCAGAU GUGAAAUCCC CGGGCUCAAC CUGGGAACU GCAUCUGAUA CUGGCAAGCU UGAGUCUCGU AGAGGGGGGU AGAAUUCCAG GUGUAGCGGU GAAAUGCGUA GAGAUCUGGA GGAAUACCG GUGGCGAAGG CGGCCCCCUG GACGAAGACU GACGCUCAGG UGCGAAAGCG UGGGGAGCAA ACAGGAUUAG A UACCCUGG UAGUCCACGC CGUAAACGAU GUCGACUUGG AGGUUGUGCC CUUGAGGCGU GGCUUCCGGA GCUAACGCGU UA AGUCGAC CGCCUGGGGA GUACGGCCGC AAGGUUAAAA CUCAAAUGAA UUGACGGGGG CCCGCACAAG CGGUGGAGCA UGU GGUUUA AUUCGAU(2MG)(5MC)A ACGCGAAGAA CCUUACCUGG UCUUGACAUC CACGGAAGUU UUCAGAGAUG AGAAUG UGC CUUCGGGAAC CGUGAGACAG GUGCUGCAUG GCUGUCGUCA GCUCGUGUUG UGAAAUGUUG GGUUAAGUCC CGCAACG AG CGCAACCCUU AUCCUUUGUU GCCAGCGGUC CGGCCGGGAA CUCAAAGGAG ACUGCCAGUG AUAAACUGGA GGAAGGUG G GGAUGACGUC AAGUCAUCAU G(2MG)CCCUUACG ACCAGGGCUA CACACGUGCU ACAAUGGCGC AUACAAAGAG AAGCG ACCU CGCGAGAGCA AGCGGACCUC AUAAAGUGCG UCGUAGUCCG GAUUGGAGUC UGCAACUCGA CUCCAUGAAG UCGGAA UCG CUAGUAAUCG UGGAUCAGAA UGCCACGGUG AAUACGUUCC CGGGCCUUGU ACACACCG(4OC)C CGU(5MC)ACACC AUGGGAGUGG GUUGCAAAAG AAGUAGGUAG CUUAACCUUC GGGAGGGCGC UUACCACUUU GUGAUUCAUG ACUGGGGUGA AGUCG(UR3)AAC AAGGUAACCG UAGG(2MG)G(MA6)(MA6)CC UGCGGUUGGA UCACCUCCUU ACCUUAAAGA AGC

GENBANK: GENBANK: CP014269.1

Macromolecule #26: mRNA

• Macromolecule: Name: mRNA / type: rna / ID: 26 / Number of copies: 1
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 1.923237 KDa

Sequence

String:

AAAGUA

Macromolecule #27: P-site tRNA

• Macromolecule: Name: P-site tRNA / type: rna / ID: 27 / Number of copies: 1
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 24.842811 KDa

Sequence

String:

GGCGGGGUGG AGCAGCCUGG UAGCUCGUCG GGCUUACAAC CCGAAGGUCG UCGGUUCAAA UCCGGCCCCC GCAACCA

GENBANK: GENBANK: AP022351.1

Macromolecule #28: 23S rRNA

• Macromolecule: Name: 23S rRNA / type: rna / ID: 28 / Number of copies: 1
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 948.625938 KDa

Sequence

String:

GGUUAAGCGA CUAAGCGUAC ACGGUGGAUG CCCUGGCAGU CAGAGGCGAU GAAGGACGUG CUAAUCUGCG AUAAGCGUCG GUAAGGUGA UAUGAACCGU UAUAACCGGC GAUUUCCGAA UGGGGAAACC CAGUGUGUUU CGACACACUA UCAUUAACUG A AUCCAUAG GUUAAUGAGG CGAACCGGGG GAACUGAAAC AUCUAAGUAC CCCGAGGAAA AGAAAUCAAC CGAGAUUCCC CC AGUAGCG GCGAGCGAAC GGGGAGCAGC CCAGAGCCUG AAUCAGUGUG UGUGUUAGUG GAAGCGUCUG GAAAGGCGCG CGA UACAGG GUGACAGCCC CGUACACAAA AAUGCACAUG CUGUGAGCUC GAUGAGUAGG GCGGGACACG UGGUAUCCUG UCUG AAUAU GGGGGGACCA UCCUCCAAGG CUAAAUACUC CUGACUGACC GAUAGUGAAC CAGUACCGUG AGGGAAAGGC GAAAA GAAC CCCGGCGAGG GGAGUGAAAA AGAACCUGAA ACCGUGUACG UACAAGCAGU GGGAGCACAG AGCAAUCUGU GUGACU GCG UACCUUUUGU AUAAUGGGUC AGCGACUUAU AUUCUGUAGC AAGGUUAACC GAAUAGGGGA GCCGAAGGGA AACCGAG UC UUAACUGGGC GUUAAGUUGC AGGGUAUAGA CCCGAAACCC GGUGAUCUAG CCAUGGGCAG GUUGAAGGUU GGGUAACA C UAACUGGAGG ACCGAACCGA CUAAU(1MG)(PSU)(5MU)GA AAAAUUAGCG GAUGACUUGU GGCUGGGGGU GAAAGGC CA AUCAAACCGG GAGAUAGCUG GUUCUCCCCG AAAGCUAUUU AGGUAGCGCC UCGUGAAUUC AUCUCCGGGG GUAGAGCA C UGUUUCGGCA AGGGGGUCAU CCCGACUUAC CAACCCGAUG CAAACUGCGA AUACCGGAGA AUGUUAUCAC GGGAGACAC ACGGCGGG(PSU)G CUAACGUCCG UCGUGAAGAG GGAAACAACC CAGACCGCCA GCUAAGGUCC CAAAGUCAUG GUUAAG UGG GAAACGAUGU GGGAAGGCCC AGACAGCCAG GAUGUUGGCU UAGAAGCAGC CAUCAUUUAA AGAAAGCGUA AUAGCUC AC UGGUCGAGUC GGCCUGCGCG GAAGAUGUAA CGGGGCUAAA CCAUGCACCG AAGCUGCGGC AGCGACGCUU AUGCGUUG U UGGGUAGGGG AGCGUUCUGU AAGCCUGUGA AGGUGUGCUG UGAGGCAUGC UGGAGGUAUC AGAAGUGCGA AUGCUGACA UAAGUAACGA UAAAGCGGGU GAAAAGCCCG CUCGCCGGAA GACCAAGGGU UCCUGUCCAA CGUUAAUCGG GGCAGGGUGA GUCGACCCC UAAGGCGAGG CCGAAAGGCG UAGUCGAUGG GAAACAGGUU AAUAUUCCUG UACUUGGUGU UACUGCGAAG G GGGGACGG AGAAGGCUAU GUUGGCCGGG CGACGGUUGU CCCGGUUUAA GCGUGUAGGC UGGUUUUCCA GGCAAAUCCG GA AAAUCAA GGCUGAGGCG UGAUGACGAG GCACUACGGU GCUGAAGCAA CAAAUGCCCU GCUUCCAGGA AAAGCCUCUA AGC AUCAGG UAACAUCAAA UCGUACCCCA AACCGACAC(6MZ) GGUGGUCAGG UAGAGAAUAC CAAGGCGCUU GAGAGAACUC GGGUGAAGG AACUAGGCAA AAUGGUGCCG UAACUUCGGG AGAAGGCACG CUGAUAUGUA GGUGAGGUCC CUCGCGGAUG G AGCUGAAA UCAGUCGAAG AUACCAGCUG GCUGCAACUG UUUAUUAAAA ACACAGCACU GUGCAAACAC GAAAGUGGAC GU AUACGGU GUGACGCCU(2MG) CCCGGUGCCG GAAGGUUAAU UGAUGGGGUU AGCGGUAACG CGAAGCUCUU GAUCGAAGC CCCGGUAAAC GGCGGCCG(PSU)A AC(3TD)A(PSU)AACGG UCCUAAGGUA GCGAAA(5MU)UCC UUGUCGGGUA AGUU CCGAC (5MC)UGCACGAAU GGCGUAAUGA UGGCCAGGCU GUCUCCACCC GAGACUCAGU GAAAUUGAAC UCGCUGUG (6MZ) AGAUGCAGUG UACCCGCGGC AAGACGGAAA GACCCCGU(G7M)A ACCUUUACUA UAGCUUGACA CUGAACAUUG A GCCUUGAU GUGUAGGAUA GGUGGGAGGC UUUGAAGUGU GGACGCCAGU CUGCAUGGAG CCGGCCUUGA AAUACCACCC UU UAAUGUU UGAUGUUCUA ACGUUGGCCC CUGAUCGGGG UUGCGGACAG UGUCUGGUGG GUAGUUUGAC UG(OMG)GGCGGU CUCCUCCUAA AGAGUAACGG AGGAGCACGA AGGUUGGCUA AUCCUGGUCG GACAUCAGGA GGUUAGUGCA AUGGCAUAAG CCAGCUUGA CUGCGAGCGU GACGGCGCGA GCAGGUGCGA AAGCAGGUCA UAGUGAUCCG GUGGUUCUGA AUGGAAGGGC C AUCGCUCA ACGGAUAAAA GGUACUCCG(2MG) GGA(H2U)AACAGG C(PSU)GAUACCGC CCAAGAGUUC AUAUCGACGG CGGUGUUUG GCA(OMC)CUCG(2MA)(PSU) GUCGGCUCAU CACAUCCUGG GGCUGAAGUA GGUCCCAAGG GUAUGGC (OMU)G UUCGCCAUUU AAAGUGGUAC GCGAGC(PSU)GGG UUUAGAACGU CGUGAGACAG (PSU)(PSU)CGGUCCCU AU CUGCCGU GGGCGCUGGA GAACUGAGGG GGGCUGCUCC UAGUACGAGA GGACCGGAGU GGACGCAUCA CUGGUGUUCG GGU UGUCAU GCCAAUGGCA CUGCCCGGUA GCUAAAUGCG GAAGAGAUAA GUGCUGAAAG CAUCUAAGCA CGAAACUUGC CCCG AGAUG AGUUCUCCCU GACUCCUUGA GAGUCCUGAA GGAACGUUGA AGACGACGAC GUUGAUAGGC CGGGUGUGUA AGCGC AGCG AUGCGUUGAG CUAACCGGUA CUAAUGAACC GUGAGGCUUA ACCUUACAAC GCCGAAGCUG UU

Macromolecule #29: 5S rRNA

• Macromolecule: Name: 5S rRNA / type: rna / ID: 29 / Number of copies: 1
• Source (natural): Organism: Escherichia coli B (bacteria)
• Molecular weight: Theoretical: 38.483926 KDa

Sequence

String:

UGCCUGGCGG CCGUAGCGCG GUGGUCCCAC CUGACCCCAU GCCGAACUCA GAAGUGAAAC GCCGUAGCGC CGAUGGUAGU GUGGGGUCU CCCCAUGCGA GAGUAGGGAA CUGCCAGGCA

GENBANK: GENBANK: CP053605.1

Macromolecule #54: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 54 / Number of copies: 1 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #55: POTASSIUM ION

• Macromolecule: Name: POTASSIUM ION / type: ligand / ID: 55 / Number of copies: 168 / Formula: K
• Molecular weight: Theoretical: 39.098 Da

Macromolecule #56: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 56 / Number of copies: 361 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #57: water

• Macromolecule: Name: water / type: ligand / ID: 57 / Number of copies: 11461 / Formula: HOH
• Molecular weight: Theoretical: 18.015 Da

Chemical component information

ChemComp-HOH:
WATER / Water

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 0.5 mg/mL

Buffer

pH: 7.4
Component:

Concentration	Name
50.0 mM	HEPES
100.0 mM	KOAc
25.0 mM	Mg(OAc)2
1.0 mM	DTT

• Grid: Model: Quantifoil R2/1 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 0.2 / Pretreatment - Type: PLASMA CLEANING / Pretreatment - Time: 30 sec. / Pretreatment - Atmosphere: OTHER / Details: Fischione 1070
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 279 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: FEI TITAN KRIOS
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: C2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 165000
• Specialist optics: Energy filter - Name: TFS Selectris X / Energy filter - Slit width: 10 eV
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Image recording: Film or detector model: FEI FALCON IV (4k x 4k) / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Number grids imaged: 1 / Number real images: 19449 / Average exposure time: 5.2 sec. / Average electron dose: 40.0 e/Ų; Details: saved in EER format; 16 exposures per hole/stage movement. 11 in outer ring around the hole edge, 5 in inner ring around the hole center.
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 1579392
• Startup model: Type of model: NONE
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC / Details: Ab-initio reconstruction in cryoSPARC
• Final 3D classification: Software - Name: cryoSPARC; Details: 3D Variability analysis in cryoSPARC; analyzed in cluster mode
• Final angle assignment: Type: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC / Details: as implemented in cryoSPARC Homogeneous Refinement
• Final reconstruction: Number classes used: 2 / Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 1.55 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 506020

Atomic model buiding 1

Initial model

PDB ID:

7k00

Chain - Source name: PDB / Chain - Initial model type: experimental model

• Refinement: Space: REAL / Protocol: FLEXIBLE FIT

Output model

PDB-8b0x:
Translating 70S ribosome in the unrotated state (P and E, tRNAs)