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- EMDB-15581: BA.1 SARS-CoV-2 Spike bound to mouse ACE2 (three up, full) -

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Basic information

Entry
Database: EMDB / ID: EMD-15581
TitleBA.1 SARS-CoV-2 Spike bound to mouse ACE2 (three up, full)
Map dataBA13
Sample
  • Complex: BA.1 SARS-CoV-2 Spike bound to mouse ACE2
Biological speciesSevere acute respiratory syndrome coronavirus 2
Methodsingle particle reconstruction / cryo EM / Resolution: 3.03 Å
AuthorsLau K / Ni D / Beckert B / Nazarov S / Myasnikov A / Pojer F / Stahlberg H / Uchikawa E
Funding support Switzerland, 1 items
OrganizationGrant numberCountry
Swiss National Science FoundationNCCR transcure Switzerland
Citation
Journal: Plos Pathog. / Year: 2023
Title: Cryo-EM structures and binding of mouse and human ACE2 to SARS-CoV-2 variants of concern indicate that mutations enabling immune escape could expand host range
Authors: Ni D / Turelli P / Beckert B / Nazarov S / Uchikawa E / Myasnikov A / Pojer F / Trono D / Stahlberg H / Lau K
#1: Journal: Biorxiv / Year: 2023
Title: Cryo-EM structures and binding of mouse and human ACE2 to SARS-CoV-2 variants of concern indicate that mutations enabling immune escape could expand host range
Authors: Ni D / Turelli P / Beckert B / Nazarov S / Uchikawa E / Myasnikov A / Pojer F / Trono D / Stahlberg H / Lau K
History
DepositionAug 11, 2022-
Header (metadata) releaseMar 1, 2023-
Map releaseMar 1, 2023-
UpdateMar 15, 2023-
Current statusMar 15, 2023Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_15581.png

Downloads & links

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Map

FileDownload / File: emd_15581.map.gz / Format: CCP4 / Size: 59.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationBA13
Voxel sizeX=Y=Z: 1.452 Å
Density
Contour LevelBy AUTHOR: 0.0824
Minimum - Maximum-0.1823121 - 0.62305677
Average (Standard dev.)0.000140098 (±0.024023995)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions250250250
Spacing250250250
CellA=B=C: 363.0 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: BA13

Fileemd_15581_half_map_1.map
AnnotationBA13
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: BA13

Fileemd_15581_half_map_2.map
AnnotationBA13
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : BA.1 SARS-CoV-2 Spike bound to mouse ACE2

EntireName: BA.1 SARS-CoV-2 Spike bound to mouse ACE2
Components
  • Complex: BA.1 SARS-CoV-2 Spike bound to mouse ACE2

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Supramolecule #1: BA.1 SARS-CoV-2 Spike bound to mouse ACE2

SupramoleculeName: BA.1 SARS-CoV-2 Spike bound to mouse ACE2 / type: complex / ID: 1 / Chimera: Yes / Parent: 0
Source (natural)Organism: Severe acute respiratory syndrome coronavirus 2
Molecular weightTheoretical: 430 KDa

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE
DetailsBA.1 SARS-CoV-2 Spike bound to mouse ACE2

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.8000000000000003 µm / Nominal defocus min: 0.8 µm
Image recordingFilm or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 58.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Initial angle assignmentType: ANGULAR RECONSTITUTION
Final angle assignmentType: ANGULAR RECONSTITUTION
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.03 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 24621
FSC plot (resolution estimation)

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