- EMDB-13324: Central row of the protein scaffold at rod outer segment disk rim... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-13324
タイトル
Central row of the protein scaffold at rod outer segment disk rims in ABCA4 knockout mice (Volta phase plate data).
マップデータ
Protein scaffold located at the outer periphery of rod outer segment disk rims in ABCA4 ko mice. The alignment mask focused on 4 repeats along the central row. Used here was a phase plate dataset.
ジャーナル: Elife / 年: 2021 タイトル: Determinants shaping the nanoscale architecture of the mouse rod outer segment. 著者: Matthias Pöge / Julia Mahamid / Sanae S Imanishi / Jürgen M Plitzko / Krzysztof Palczewski / Wolfgang Baumeister / 要旨: The unique membrane organization of the rod outer segment (ROS), the specialized sensory cilium of rod photoreceptor cells, provides the foundation for phototransduction, the initial step in vision. ...The unique membrane organization of the rod outer segment (ROS), the specialized sensory cilium of rod photoreceptor cells, provides the foundation for phototransduction, the initial step in vision. ROS architecture is characterized by a stack of identically shaped and tightly packed membrane disks loaded with the visual receptor rhodopsin. A wide range of genetic aberrations have been reported to compromise ROS ultrastructure, impairing photoreceptor viability and function. Yet, the structural basis giving rise to the remarkably precise arrangement of ROS membrane stacks and the molecular mechanisms underlying genetically inherited diseases remain elusive. Here, cryo-electron tomography (cryo-ET) performed on native ROS at molecular resolution provides insights into key structural determinants of ROS membrane architecture. Our data confirm the existence of two previously observed molecular connectors/spacers which likely contribute to the nanometer-scale precise stacking of the ROS disks. We further provide evidence that the extreme radius of curvature at the disk rims is enforced by a continuous supramolecular assembly composed of peripherin-2 (PRPH2) and rod outer segment membrane protein 1 (ROM1) oligomers. We suggest that together these molecular assemblies constitute the structural basis of the highly specialized ROS functional architecture. Our Cryo-ET data provide novel quantitative and structural information on the molecular architecture in ROS and substantiate previous results on proposed mechanisms underlying pathologies of certain PRPH2 mutations leading to blindness.
EMPIAR-10771 (タイトル: Cryo-electron tomography of rod outer segments in ABCA4 knockout mice acquired with Volta phase plate in focus Data size: 3.0 Data #1: Tomograms of rod outer segments in ABCA4 knockout mice acquired with Volta phase plate [reconstructed volumes])
ダウンロード / ファイル: emd_13324.map.gz / 形式: CCP4 / 大きさ: 8 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
Protein scaffold located at the outer periphery of rod outer segment disk rims in ABCA4 ko mice. The alignment mask focused on 4 repeats along the central row. Used here was a phase plate dataset.
名称: Rod outer segment disk rim scaffold / タイプ: complex / ID: 1 / 親要素: 0 詳細: Protein scaffold located at the outer periphery of rod outer segment disk rims in ABCA4 ko mice - the central row.