+Open data
-Basic information
Entry | Database: PDB / ID: 1ar0 | ||||||
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Title | NUCLEAR TRANSPORT FACTOR 2 (NTF2) E42K MUTANT | ||||||
Components | NUCLEAR TRANSPORT FACTOR 2 | ||||||
Keywords | TRANSPORT / NUCLEAR TRANSPORT PROTEIN | ||||||
Function / homology | Function and homology information negative regulation of vascular endothelial growth factor production / protein localization to nuclear pore / nuclear pore central transport channel / structural constituent of nuclear pore / nuclear outer membrane / nuclear inner membrane / nuclear import signal receptor activity / mRNA transport / protein export from nucleus / small GTPase binding ...negative regulation of vascular endothelial growth factor production / protein localization to nuclear pore / nuclear pore central transport channel / structural constituent of nuclear pore / nuclear outer membrane / nuclear inner membrane / nuclear import signal receptor activity / mRNA transport / protein export from nucleus / small GTPase binding / positive regulation of protein import into nucleus / protein import into nucleus / nuclear membrane / nucleoplasm / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.3 Å | ||||||
Authors | Mccoy, A.J. / Stewart, M.J. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1997 Title: Nuclear protein import is decreased by engineered mutants of nuclear transport factor 2 (NTF2) that do not bind GDP-Ran. Authors: Clarkson, W.D. / Corbett, A.H. / Paschal, B.M. / Kent, H.M. / McCoy, A.J. / Gerace, L. / Silver, P.A. / Stewart, M. #1: Journal: J.Mol.Biol. / Year: 1996 Title: The 1.6 Angstroms Resolution Crystal Structure of Nuclear Transport Factor 2 (Ntf2) Authors: Bullock, T.L. / Clarkson, W.D. / Kent, H.M. / Stewart, M. #2: Journal: Structure / Year: 1994 Title: Crystal Structure of Scytalone Dehydratase--A Disease Determinant of the Rice Pathogen, Magnaporthe Grisea Authors: Lundqvist, T. / Rice, J. / Hodge, C.N. / Basarab, G.S. / Pierce, J. / Lindqvist, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ar0.cif.gz | 60.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ar0.ent.gz | 46.2 KB | Display | PDB format |
PDBx/mmJSON format | 1ar0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1ar0_validation.pdf.gz | 426.2 KB | Display | wwPDB validaton report |
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Full document | 1ar0_full_validation.pdf.gz | 429.8 KB | Display | |
Data in XML | 1ar0_validation.xml.gz | 12.1 KB | Display | |
Data in CIF | 1ar0_validation.cif.gz | 16 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ar/1ar0 ftp://data.pdbj.org/pub/pdb/validation_reports/ar/1ar0 | HTTPS FTP |
-Related structure data
Related structure data | 1askC 1ounS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.914096, 0.124306, 0.385975), Vector: |
-Components
#1: Protein | Mass: 14491.491 Da / Num. of mol.: 2 / Mutation: E42K Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Organ: LIVER / Plasmid: PET VECTOR / Production host: Escherichia coli (E. coli) / Strain (production host): PET / References: UniProt: P61972 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.47 Å3/Da / Density % sol: 47 % | |||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 4.5 / Details: pH 4.5 | |||||||||||||||||||||||||||||||||||||||||||||
Crystal | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion / Details: Kent, H.M., (1996) J. Struct. Biol., 116, 325. | |||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ROTATING ANODE / Type: ELLIOTT GX-13 / Wavelength: 1.5418 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Nov 1, 1996 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→100 Å / Num. obs: 13342 / % possible obs: 99.5 % / Observed criterion σ(I): 0 / Redundancy: 4.2 % / Rmerge(I) obs: 0.077 / Net I/σ(I): 6.5 |
Reflection shell | Resolution: 2.3→2.42 Å / Redundancy: 4 % / Rmerge(I) obs: 0.217 / Mean I/σ(I) obs: 3.4 / % possible all: 98.1 |
Reflection shell | *PLUS % possible obs: 98.1 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: NTF2 (PDB ENTRY 1OUN) Resolution: 2.3→20 Å / Cross valid method: THROUGHOUT / σ(F): 0 Details: ASP 92 IN BOTH CHAINS WAS DISTORTED BY BEING IN AN EXTREMELY TIGHT SURFACE LOOP AND, ALTHOUGH IT HAD UNFAVORABLE PHI/PSI ANGLES, THE MODEL FITTED THE ELECTRON DENSITY CLOSELY.
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Refinement step | Cycle: LAST / Resolution: 2.3→20 Å
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||
Refinement | *PLUS Rfactor Rfree: 0.228 / Rfactor Rwork: 0.2 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS
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