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- EMDB-8697: Closed State CryoEM Reconstruction of Hsp104:ATPyS and FITC casein -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-8697 | |||||||||
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Title | Closed State CryoEM Reconstruction of Hsp104:ATPyS and FITC casein | |||||||||
![]() | Closed State of Hsp104:ATPyS and FITC casein | |||||||||
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Function / homology | ![]() trehalose metabolism in response to heat stress / TRC complex / cellular heat acclimation / protein folding in endoplasmic reticulum / post-translational protein targeting to endoplasmic reticulum membrane / stress granule disassembly / chaperone cofactor-dependent protein refolding / protein unfolding / nuclear periphery / ADP binding ...trehalose metabolism in response to heat stress / TRC complex / cellular heat acclimation / protein folding in endoplasmic reticulum / post-translational protein targeting to endoplasmic reticulum membrane / stress granule disassembly / chaperone cofactor-dependent protein refolding / protein unfolding / nuclear periphery / ADP binding / unfolded protein binding / protein-folding chaperone binding / cellular response to heat / protein refolding / ATP hydrolysis activity / ATP binding / identical protein binding / nucleus / cytosol / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() ![]() ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 4.0 Å | |||||||||
![]() | Gates SN / Yokom AL / Lin J-B / Jackrel ME / Rizo AN / Kendsersky NM / Buell CE / Sweeny EA / Chuang E / Torrente MP ...Gates SN / Yokom AL / Lin J-B / Jackrel ME / Rizo AN / Kendsersky NM / Buell CE / Sweeny EA / Chuang E / Torrente MP / Mack KL / Su M / Shorter J / Southworth DR | |||||||||
![]() | ![]() Title: Ratchet-like polypeptide translocation mechanism of the AAA+ disaggregase Hsp104. Authors: Stephanie N Gates / Adam L Yokom / JiaBei Lin / Meredith E Jackrel / Alexandrea N Rizo / Nathan M Kendsersky / Courtney E Buell / Elizabeth A Sweeny / Korrie L Mack / Edward Chuang / Mariana ...Authors: Stephanie N Gates / Adam L Yokom / JiaBei Lin / Meredith E Jackrel / Alexandrea N Rizo / Nathan M Kendsersky / Courtney E Buell / Elizabeth A Sweeny / Korrie L Mack / Edward Chuang / Mariana P Torrente / Min Su / James Shorter / Daniel R Southworth / ![]() Abstract: Hsp100 polypeptide translocases are conserved members of the AAA+ family (adenosine triphosphatases associated with diverse cellular activities) that maintain proteostasis by unfolding aberrant and ...Hsp100 polypeptide translocases are conserved members of the AAA+ family (adenosine triphosphatases associated with diverse cellular activities) that maintain proteostasis by unfolding aberrant and toxic proteins for refolding or proteolytic degradation. The Hsp104 disaggregase from solubilizes stress-induced amorphous aggregates and amyloids. The structural basis for substrate recognition and translocation is unknown. Using a model substrate (casein), we report cryo-electron microscopy structures at near-atomic resolution of Hsp104 in different translocation states. Substrate interactions are mediated by conserved, pore-loop tyrosines that contact an 80-angstrom-long unfolded polypeptide along the axial channel. Two protomers undergo a ratchet-like conformational change that advances pore loop-substrate interactions by two amino acids. These changes are coupled to activation of specific nucleotide hydrolysis sites and, when transmitted around the hexamer, reveal a processive rotary translocation mechanism and substrate-responsive flexibility during Hsp104-catalyzed disaggregation. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 6.4 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 13 KB 13 KB | Display Display | ![]() |
Images | ![]() | 64.5 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5vjhMC ![]() 8744C ![]() 8745C ![]() 8746C ![]() 5vy8C ![]() 5vy9C ![]() 5vyaC C: citing same article ( M: atomic model generated by this map |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Annotation | Closed State of Hsp104:ATPyS and FITC casein | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Hsp104 ATPyS and FITC casein in Closed State
Entire | Name: Hsp104 ATPyS and FITC casein in Closed State |
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Components |
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-Supramolecule #1: Hsp104 ATPyS and FITC casein in Closed State
Supramolecule | Name: Hsp104 ATPyS and FITC casein in Closed State / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 |
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Source (natural) | Organism: ![]() ![]() |
Recombinant expression | Organism: ![]() ![]() |
-Macromolecule #1: Heat shock protein 104
Macromolecule | Name: Heat shock protein 104 / type: protein_or_peptide / ID: 1 / Number of copies: 6 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() Strain: ATCC 204508 / S288c |
Molecular weight | Theoretical: 102.173961 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MNDQTQFTER ALTILTLAQK LASDHQHPQL QPIHILAAFI ETPEDGSVPY LQNLIEKGRY DYDLFKKVVN RNLVRIPQQQ PAPAEITPS YALGKVLQDA AKIQKQQKDS FIAQDHILFA LFNDSSIQQI FKEAQVDIEA IKQQALELRG NTRIDSRGAD T NTPLEYLS ...String: MNDQTQFTER ALTILTLAQK LASDHQHPQL QPIHILAAFI ETPEDGSVPY LQNLIEKGRY DYDLFKKVVN RNLVRIPQQQ PAPAEITPS YALGKVLQDA AKIQKQQKDS FIAQDHILFA LFNDSSIQQI FKEAQVDIEA IKQQALELRG NTRIDSRGAD T NTPLEYLS KYAIDMTEQA RQGKLDPVIG REEEIRSTIR VLARRIKSNP CLIGEPGIGK TAIIEGVAQR IIDDDVPTIL QG AKLFSLD LAALTAGAKY KGDFEERFKG VLKEIEESKT LIVLFIDEIH MLMGNGKDDA ANILKPALSR GQLKVIGATT NNE YRSIVE KDGAFERRFQ KIEVAEPSVR QTVAILRGLQ PKYEIHHGVR ILDSALVTAA QLAKRYLPYR RLPDSALDLV DISC AGVAV ARDSKPEELD SKERQLQLIQ VEIKALERDE DADSTTKDRL KLARQKEASL QEELEPLRQR YNEEKHGHEE LTQAK KKLD ELENKALDAE RRYDTATAAD LRYFAIPDIK KQIEKLEDQV AEEERRAGAN SMIQNVVDSD TISETAARLT GIPVKK LSE SENEKLIHME RDLSSEVVGQ MDAIKAVSNA VRLSRSGLAN PRQPASFLFL GLSGSGKTEL AKKVAGFLFN DEDMMIR VD CSELSEKYAV SKLLGTTAGY VGYDEGGFLT NQLQYKPYSV LLFDEVEKAH PDVLTVMLQM LDDGRITSGQ GKTIDCSN C IVIMTSNLGA EFINSQQGSK IQESTKNLVM GAVRQHFRPE FLNRISSIVI FNKLSRKAIH KIVDIRLKEI EERFEQNDK HYKLNLTQEA KDFLAKYGYS DDMGARPLNR LIQNEILNKL ALRILKNEIK DKETVNVVLK KGKSRDENVP EEAEECLEVL PNHEATIGA DTLGDDDNED SMEIDDDLD |
-Macromolecule #2: FITC casein
Macromolecule | Name: FITC casein / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 2.230741 KDa |
Sequence | String: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) |
-Macromolecule #3: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER
Macromolecule | Name: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / type: ligand / ID: 3 / Number of copies: 12 / Formula: AGS |
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Molecular weight | Theoretical: 523.247 Da |
Chemical component information | ![]() ChemComp-AGS: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.5 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 0.8 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal magnification: 50000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Details | Removed first 2 frames from movie before drift correction |
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Final reconstruction | Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 4.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 146463 |
Initial angle assignment | Type: ANGULAR RECONSTITUTION |
Final angle assignment | Type: ANGULAR RECONSTITUTION |