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- EMDB-8480: Cryo-EM map of the Mediator-RNAPII complex -

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Basic information

Entry
Database: EMDB / ID: EMD-8480
TitleCryo-EM map of the Mediator-RNAPII complex
Map dataMediator-RNAPII complex
Sample
  • Complex: complex of Mediator and RNAPII
    • Protein or peptide: x 28 types
Function / homology
Function and homology information


RNA Polymerase I Transcription Initiation / RNA polymerase II transcribes snRNA genes / Processing of Capped Intron-Containing Pre-mRNA / Formation of TC-NER Pre-Incision Complex / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / Estrogen-dependent gene expression / RNA Polymerase I Promoter Escape ...RNA Polymerase I Transcription Initiation / RNA polymerase II transcribes snRNA genes / Processing of Capped Intron-Containing Pre-mRNA / Formation of TC-NER Pre-Incision Complex / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / Estrogen-dependent gene expression / RNA Polymerase I Promoter Escape / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / mRNA Capping / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / RNA Polymerase II Pre-transcription Events / TP53 Regulates Transcription of DNA Repair Genes / RNA Polymerase II Transcription Elongation / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / regulation of septum digestion after cytokinesis / regulatory ncRNA-mediated heterochromatin formation / DNA-templated transcription elongation / core mediator complex / mediator complex / : / : / positive regulation of nuclear-transcribed mRNA poly(A) tail shortening / termination of RNA polymerase II transcription / maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II / tRNA transcription by RNA polymerase III / transcription elongation by RNA polymerase I / positive regulation of translational initiation / RNA polymerase II activity / transcription-coupled nucleotide-excision repair / positive regulation of transcription initiation by RNA polymerase II / RNA polymerase I complex / transcription by RNA polymerase I / RNA polymerase III complex / transcription by RNA polymerase III / RNA polymerase II, core complex / pericentric heterochromatin / translation initiation factor binding / P-body / transcription initiation at RNA polymerase II promoter / transcription coregulator activity / euchromatin / ribonucleoside binding / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / single-stranded DNA binding / transcription by RNA polymerase II / single-stranded RNA binding / nucleic acid binding / transcription coactivator activity / protein dimerization activity / RNA polymerase II cis-regulatory region sequence-specific DNA binding / nucleotide binding / chromatin / nucleolus / regulation of transcription by RNA polymerase II / DNA binding / RNA binding / zinc ion binding / metal ion binding / nucleus / cytosol / cytoplasm
Similarity search - Function
Mediator complex, subunit Med27 / Mediator complex subunit 27 / Mediator complex, subunit Med6, fungi / Mediator complex, subunit Med7 superfmaily / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / SOH1 / Subunit 21 of Mediator complex / Mediator complex, subunit Med7 ...Mediator complex, subunit Med27 / Mediator complex subunit 27 / Mediator complex, subunit Med6, fungi / Mediator complex, subunit Med7 superfmaily / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / SOH1 / Subunit 21 of Mediator complex / Mediator complex, subunit Med7 / Mediator complex, subunit Med7/Med21-like / MED7 protein / Mediator complex, subunit Med20 / Mediator complex, subunit Med14 / TATA-binding related factor (TRF) of subunit 20 of Mediator complex / Mediator complex subunit MED14 / Mediator complex, subunit Med6 / Mediator complex, subunit Med17 / Mediator complex, subunit Med6 superfamily / MED6 mediator sub complex component / Subunit 17 of Mediator complex / Mediator of RNA polymerase II transcription subunit 22 / Mediator complex, subunit Med8, fungi/metazoa / Mediator complex, subunit Med11 / Surfeit locus protein 5 subunit 22 of Mediator complex / Mediator of RNA polymerase II transcription complex subunit 8 / Mediator complex protein / Mediator complex, subunit Med18 / Med18 protein / DNA-directed RNA polymerase II subunit Rpb4-like / RNA polymerase Rpb4/RPC9, core / DNA-directed RNA-polymerase II subunit / RNA polymerase II, heptapeptide repeat, eukaryotic / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb1, domain 6 / RNA polymerase Rpb1 C-terminal repeat / Eukaryotic RNA polymerase II heptapeptide repeat. / RNA polymerase Rpb1, domain 7 / RNA polymerase Rpb1, domain 7 superfamily / RNA polymerase Rpb1, domain 7 / Pol II subunit B9, C-terminal zinc ribbon / RNA polymerase RBP11 / Rpb4/RPC9 superfamily / RNA polymerase subunit Rpb4/RPC9 / RNA polymerase Rpb4 / Zinc finger TFIIS-type signature. / HRDC-like superfamily / RNA polymerase subunit Rpb7-like / RNA polymerase Rpb7-like , N-terminal / RNA polymerase Rpb7-like, N-terminal domain superfamily / SHS2 domain found in N terminus of Rpb7p/Rpc25p/MJ0397 / RNA polymerase Rpb2, domain 4 / RNA polymerase Rpb2, domain 4 / RNA polymerase Rpb2, domain 5 / RNA polymerase Rpb2, domain 5 / DNA-directed RNA polymerase subunit RPABC5/Rpb10 / RNA polymerases, subunit N, zinc binding site / RNA polymerase subunit RPB10 / RNA polymerases N / 8 kDa subunit / RNA polymerases N / 8 Kd subunits signature. / DNA-directed RNA polymerase, M/15kDa subunit / RNA polymerases M/15 Kd subunit / RNA polymerase subunit 9 / DNA-directed RNA polymerase M, 15kDa subunit, conserved site / RNA polymerases M / 15 Kd subunits signature. / DNA-directed RNA polymerase subunit/transcription factor S / RNA polymerase, Rpb8 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / RNA polymerase Rpb8 / RNA polymerase subunit 8 / RNA polymerase, Rpb5, N-terminal / RNA polymerase Rpb5, N-terminal domain superfamily / RNA polymerase Rpb5, N-terminal domain / DNA-directed RNA polymerase, subunit RPB6 / DNA-directed RNA polymerase subunit Rpo11 / DNA directed RNA polymerase, 7 kDa subunit / RNA polymerase archaeal subunit P/eukaryotic subunit RPABC4 / RNA polymerase, subunit H/Rpb5, conserved site / RNA polymerases H / 23 Kd subunits signature. / RNA polymerase subunit CX / DNA-directed RNA polymerase Rpb11, 13-16kDa subunit, conserved site / DNA-directed RNA polymerase subunit Rpo5/Rpb5 / RNA polymerases L / 13 to 16 Kd subunits signature. / DNA-directed RNA polymerase, 30-40kDa subunit, conserved site / DNA-directed RNA polymerase subunit Rpo3/Rpb3/RPAC1 / RNA polymerases D / 30 to 40 Kd subunits signature. / DNA-directed RNA polymerase, RBP11-like dimerisation domain / RNA polymerase Rpb3/Rpb11 dimerisation domain / RNA polymerase, subunit H/Rpb5 C-terminal / RNA polymerase subunit RPABC4/transcription elongation factor Spt4 / RPB5-like RNA polymerase subunit superfamily / RNA polymerase Rpb5, C-terminal domain / Zinc finger, TFIIS-type / Transcription factor S-II (TFIIS) / Zinc finger TFIIS-type profile. / C2C2 Zinc finger / Archaeal Rpo6/eukaryotic RPB6 RNA polymerase subunit / DNA-directed RNA polymerase, 14-18kDa subunit, conserved site / RNA polymerases K / 14 to 18 Kd subunits signature. / S1 RNA binding domain
Similarity search - Domain/homology
DNA-directed RNA polymerases I, II, and III subunit RPABC5 / Uncharacterized protein C24C9.04 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 18 / DNA-directed RNA polymerase II subunit rpb7 / Mediator of RNA polymerase II transcription subunit 7 / DNA-directed RNA polymerase II subunit RPB9 / DNA-directed RNA polymerase II subunit rpb4 / Mediator of RNA polymerase II transcription subunit 21 / Mediator of RNA polymerase II transcription subunit 8 ...DNA-directed RNA polymerases I, II, and III subunit RPABC5 / Uncharacterized protein C24C9.04 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 18 / DNA-directed RNA polymerase II subunit rpb7 / Mediator of RNA polymerase II transcription subunit 7 / DNA-directed RNA polymerase II subunit RPB9 / DNA-directed RNA polymerase II subunit rpb4 / Mediator of RNA polymerase II transcription subunit 21 / Mediator of RNA polymerase II transcription subunit 8 / DNA-directed RNA polymerase II subunit rpb1 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerase II subunit RPB3 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / DNA-directed RNA polymerase II subunit RPB11 / Mediator of RNA polymerase II transcription subunit 17 / DNA-directed RNA polymerase II subunit RPB2 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / Mediator of RNA polymerase II transcription subunit 20 / Mediator of RNA polymerase II transcription subunit 27 / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / Mediator of RNA polymerase II transcription subunit 11 / Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 6 / Mediator of RNA polymerase II transcription subunit 31
Similarity search - Component
Biological speciesSchizosaccharomyces pombe (fission yeast) / Fission yeast (fission yeast)
Methodsingle particle reconstruction / cryo EM / Resolution: 7.8 Å
AuthorsTsai K-L / Yu X / Gopalan S / Chao T-C / Zhang Y / Florens L / Washburn MP / Murakami K / Conaway RC / Conaway JW / Asturias F
Funding support United States, 2 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM67167 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM41628 United States
CitationJournal: Nature / Year: 2017
Title: Mediator structure and rearrangements required for holoenzyme formation.
Authors: Kuang-Lei Tsai / Xiaodi Yu / Sneha Gopalan / Ti-Chun Chao / Ying Zhang / Laurence Florens / Michael P Washburn / Kenji Murakami / Ronald C Conaway / Joan W Conaway / Francisco J Asturias /
Abstract: The conserved Mediator co-activator complex has an essential role in the regulation of RNA polymerase II transcription in all eukaryotes. Understanding the structure and interactions of Mediator is ...The conserved Mediator co-activator complex has an essential role in the regulation of RNA polymerase II transcription in all eukaryotes. Understanding the structure and interactions of Mediator is crucial for determining how the complex influences transcription initiation and conveys regulatory information to the basal transcription machinery. Here we present a 4.4 Å resolution cryo-electron microscopy map of Schizosaccharomyces pombe Mediator in which conserved Mediator subunits are individually resolved. The essential Med14 subunit works as a central backbone that connects the Mediator head, middle and tail modules. Comparison with a 7.8 Å resolution cryo-electron microscopy map of a Mediator-RNA polymerase II holoenzyme reveals that changes in the structure of Med14 facilitate a large-scale Mediator rearrangement that is essential for holoenzyme formation. Our study suggests that access to different conformations and crosstalk between structural elements are essential for the Mediator regulation mechanism, and could explain the capacity of the complex to integrate multiple regulatory signals.
History
DepositionNov 26, 2016-
Header (metadata) releaseMar 8, 2017-
Map releaseMar 8, 2017-
UpdateJan 1, 2020-
Current statusJan 1, 2020Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.08
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.08
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-5u0s
  • Surface level: 0.08
  • Imaged by UCSF Chimera
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  • Simplified surface model + fitted atomic model
  • Atomic modelsPDB-5u0s
  • Imaged by Jmol
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_8480.png

Downloads & links

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Map

FileDownload / File: emd_8480.map.gz / Format: CCP4 / Size: 20.8 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationMediator-RNAPII complex
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
2.62 Å/pix.
x 176 pix.
= 461.12 Å		2.62 Å/pix.
x 176 pix.
= 461.12 Å		2.62 Å/pix.
x 176 pix.
= 461.12 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 2.62 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy EMDB: 0.08 / Movie #1: 0.08
Minimum - Maximum-0.11419473 - 0.22855034
Average (Standard dev.)-0.000103825936 (±0.014926006)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions176176176
Spacing176176176
CellA=B=C: 461.12 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z2.622.622.62
M x/y/z176176176
origin x/y/z0.0000.0000.000
length x/y/z461.120461.120461.120
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS176176176
D min/max/mean-0.1140.229-0.000

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Supplemental data

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Sample components

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Entire : complex of Mediator and RNAPII

EntireName: complex of Mediator and RNAPII
Components
  • Complex: complex of Mediator and RNAPII
    • Protein or peptide: Mediator complex subunit 6Mediator (coactivator)
    • Protein or peptide: Mediator complex subunit 8Mediator (coactivator)
    • Protein or peptide: Mediator complex subunit 17Mediator (coactivator)
    • Protein or peptide: Mediator complex subunit 18Mediator (coactivator)
    • Protein or peptide: Mediator complex subunit 20Mediator (coactivator)
    • Protein or peptide: Mediator complex subunit 11Mediator (coactivator)
    • Protein or peptide: Mediator complex subunit 22Mediator (coactivator)
    • Protein or peptide: Mediator complex subunit 14Mediator (coactivator)
    • Protein or peptide: Mediator complex subunit 4Mediator (coactivator)
    • Protein or peptide: Mediator complex subunit 7Mediator (coactivator)
    • Protein or peptide: Mediator complex subunit 21Mediator (coactivator)
    • Protein or peptide: Mediator complex subunit 31Mediator (coactivator)
    • Protein or peptide: Mediator complex subunit 27Mediator (coactivator)
    • Protein or peptide: Mediator complex subunit 9Mediator (coactivator)
    • Protein or peptide: Mediator complex subunit 19Mediator (coactivator)
    • Protein or peptide: Mediator complex subunit 10Mediator (coactivator)
    • Protein or peptide: RNA polymerase II subunit Rpb1
    • Protein or peptide: RNA polymerase II subunit Rpb2
    • Protein or peptide: RNA polymerase II subunit Rpb3
    • Protein or peptide: RNA polymerase II subunit Rpb4
    • Protein or peptide: RNA polymerase II subunit Rpb5
    • Protein or peptide: RNA polymerase II subunit Rpb6
    • Protein or peptide: RNA polymerase II subunit Rpb7
    • Protein or peptide: RNA polymerase II subunit Rpb8
    • Protein or peptide: RNA polymerase II subunit Rpb9
    • Protein or peptide: RNA polymerase II subunit Rpb10
    • Protein or peptide: RNA polymerase II subunit Rpb11
    • Protein or peptide: RNA polymerase II subunit Rpb12

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Supramolecule #1: complex of Mediator and RNAPII

SupramoleculeName: complex of Mediator and RNAPII / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Schizosaccharomyces pombe (fission yeast)
Molecular weightTheoretical: 1.5 MDa

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Macromolecule #1: Mediator complex subunit 6

MacromoleculeName: Mediator complex subunit 6 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 24.928947 KDa
SequenceString: MASGAPPSVD LTSIQWRMPE WVQSMGGLRT ENVLEYFSQS PFYSHKSNNE MLKMQSQFNA LDLGDLNSQL KRLTGIQFVI IHERPPFLW VIQKQNRLNE NEVKPLTVYF VCNENIYMAP NAYTLLATRM LNATYCFQKA LTKIEKFPQY NPQEGYTYPK L SNDNLEVD ...String:
MASGAPPSVD LTSIQWRMPE WVQSMGGLRT ENVLEYFSQS PFYSHKSNNE MLKMQSQFNA LDLGDLNSQL KRLTGIQFVI IHERPPFLW VIQKQNRLNE NEVKPLTVYF VCNENIYMAP NAYTLLATRM LNATYCFQKA LTKIEKFPQY NPQEGYTYPK L SNDNLEVD HSNTNEPADE NKNQSIENAD YSFSPEDFSV VRAFMQSLHS SKEAPDVK

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Macromolecule #2: Mediator complex subunit 8

MacromoleculeName: Mediator complex subunit 8 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 23.360068 KDa
SequenceString: MEDISTEKTV ESLEAIRHRI AQIVQSLTHF LAILHQSESL SPWPTIHKNF NILLSQIHSL SNNLAAHSHT LQTTSIYPSL EFPVKEQEP LLTTLLRTKA LPEVEEWEAN TLQEYEASIS SQPKKKEAND AYQKDQLWDQ ARIIFMEERE NYSWFDFVTR R QESEGEFV ...String:
MEDISTEKTV ESLEAIRHRI AQIVQSLTHF LAILHQSESL SPWPTIHKNF NILLSQIHSL SNNLAAHSHT LQTTSIYPSL EFPVKEQEP LLTTLLRTKA LPEVEEWEAN TLQEYEASIS SQPKKKEAND AYQKDQLWDQ ARIIFMEERE NYSWFDFVTR R QESEGEFV SQRQLEIDRA TEEQNANQML TDILSFMKSG KR

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Macromolecule #3: Mediator complex subunit 17

MacromoleculeName: Mediator complex subunit 17 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 62.55141 KDa
SequenceString: MAEEANKDAD ISSLSLSLDP EIIGGQNNFL ENNLQQIFQK IIQERGPFRD LKEEDLQKEL QKESIKDESS AKSSETENVL EFATLDSKR NVNDTEVESM DSQAYKKELI EQIMIAQTEC SLALDMTSLL LSKFKENSIE TISPFLKSTV PPSSLQFSRS Q PPESKESD ...String:
MAEEANKDAD ISSLSLSLDP EIIGGQNNFL ENNLQQIFQK IIQERGPFRD LKEEDLQKEL QKESIKDESS AKSSETENVL EFATLDSKR NVNDTEVESM DSQAYKKELI EQIMIAQTEC SLALDMTSLL LSKFKENSIE TISPFLKSTV PPSSLQFSRS Q PPESKESD ATLAKCWKEK SLTSSCKFLF EAKERLTSVV ETEHEYYTEL VKVKEASWPL FNSQGSNHLS VQYSCLGGIS LG LGLIRMK PESKSFEVQS SLLYSQAALK ISILNKDRDE IGSSTWSWPS QNCNSVLLKD IYKLQEILFE MDIWNSLLQE AQS CGNQGV NFTGDEILVP ISDDHVVRIT LETSSKNTES GFTEDKKSNE DTSTNFVTIK QEKELLKCLC DTLNAIAHIL FLKH CRKSD RRSQQPELYM AIDANAPLIL RPLIFYYNLN QESLEFQRWL KQRDISFKFM PNYPWEKAKD FLELENSLSI NRLSI SWRI MVSNFEPAIF IQHTPTLHGT DKSVWRCKDQ YSSNQFSSLK NVCQYIEHHI NSLSRRSKKT E

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Macromolecule #4: Mediator complex subunit 18

MacromoleculeName: Mediator complex subunit 18 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 24.056377 KDa
SequenceString: MQELYLLGVV PSRRFEAVVN SLSKTLDGPK TILEFWVVYR PKDVPPNLPR QPDSWLRLCS NIESHDETDT EWSKNTQWSM YLEGNSEPK REDKCGIRPV NRAKLTNGSV TEFVEKMGYE FSHEYIIQGL EYFFFDTTVR IYQTLIPSQQ RSIKPPFHPM N EEQPWILH ...String:
MQELYLLGVV PSRRFEAVVN SLSKTLDGPK TILEFWVVYR PKDVPPNLPR QPDSWLRLCS NIESHDETDT EWSKNTQWSM YLEGNSEPK REDKCGIRPV NRAKLTNGSV TEFVEKMGYE FSHEYIIQGL EYFFFDTTVR IYQTLIPSQQ RSIKPPFHPM N EEQPWILH VYTHVADASN QVAMAKAEAN LTKVKTLLSA FCDLKNVRL

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Macromolecule #5: Mediator complex subunit 20

MacromoleculeName: Mediator complex subunit 20 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 22.374775 KDa
SequenceString: MPVHGVIYYS SPSMATFLSP AQDNLVRTYF AQHLKKWVVQ YKLYRNAVTP KTLEFLKQNI NPSMLACVDE ATMIDAEPEL EDIIVRTKL WNFRQSFTIE GSIYEVGSFK VAIANVLQKS VWKGILFHVT YDGTESVDLA RPIIQEFFLK CFLQNNKSVT P VYESFFNQ ...String:
MPVHGVIYYS SPSMATFLSP AQDNLVRTYF AQHLKKWVVQ YKLYRNAVTP KTLEFLKQNI NPSMLACVDE ATMIDAEPEL EDIIVRTKL WNFRQSFTIE GSIYEVGSFK VAIANVLQKS VWKGILFHVT YDGTESVDLA RPIIQEFFLK CFLQNNKSVT P VYESFFNQ PRHSLDSKLL LQLFKQRIDT VSQRT

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Macromolecule #6: Mediator complex subunit 11

MacromoleculeName: Mediator complex subunit 11 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 13.1188 KDa
SequenceString:
MTNSDDDLFS EKSTSSDTQQ VQNILELEAK IPDILSSAGK CIEAIQLNNS LEDFRKYSKE FLETVEFIST GLRRQALELE KAEVPVVSL QPKKRYASTP LSNLIFDQSS KLIPKYC

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Macromolecule #7: Mediator complex subunit 22

MacromoleculeName: Mediator complex subunit 22 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 15.396165 KDa
SequenceString:
MSSDSFQRQL VQRTNTLNSS IDNATLTILS RFQDILDIAI NEGKDKYTVA PEVYQIECHT VSMVRAVEQL LDVSRQIKSY WLTNSLSTS FPTVDYSEPD LEKVKRTLTK LQNHLLEVSL IEPEASETTE APTVSDT

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Macromolecule #8: Mediator complex subunit 14

MacromoleculeName: Mediator complex subunit 14 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 105.382461 KDa
SequenceString: MEPPAIPHIT EGFYPLPEIV ETFSHHVLQE LVSLAEVLPS MSNVEKKKKI LDWLLRSRAF TMRLLVLARW VHLSPSVHRC IDVVAFLQG QKFCFQNLVH VLQDIRYQLS FARLRNSDLV TALDILSTGT SLRLANAPTS KLYMLSESPL STKQILQTLH A LNMLIRIR ...String:
MEPPAIPHIT EGFYPLPEIV ETFSHHVLQE LVSLAEVLPS MSNVEKKKKI LDWLLRSRAF TMRLLVLARW VHLSPSVHRC IDVVAFLQG QKFCFQNLVH VLQDIRYQLS FARLRNSDLV TALDILSTGT SLRLANAPTS KLYMLSESPL STKQILQTLH A LNMLIRIR LSLYEIIPTP FQHFTIANGR CTFTVPNEFS VSLTTNSQDP KSTGISFQWI VVDFQFHLPD FSSTPAKYRV FI ELHLNEE IAAAFVLQKP ILPLIYNILH KFCLYQRLNL LSQQTFQLSR ESWLGHLRGV YDEKPPRLRL YYWPQLNVKK EGK PGKIGH YIHIFVNTQP ISAFERTLSS KRSSCEYDHF LLLVEWHHDG IVEHVPLDDH MDAQHLLLLI TQKHAQLILE QIRK ELHPN IFSEHVGGGL KIHVFDNEII VKVNSVTGRL VLSSSASPLS PPRHLRAAEK NIALNTQPPA QILNRLYFFC IQTQL LEVA QCAELHAVQG YYSFPYLTFS KGKWRKDGDS LWVLAYNVES NSWSVRLLNA AGQTLYTQDV HTTKGTLSIE SFSRLS YLL EVQILLFNVQ TACQARGMPF EYLPIPPKAL IEDDFTTYVQ TGCLCIMMPS SNEDMLPVVF VRAHDGQLIF DSRIKGK LP YQSETETEKN CYIDWRTGRI TIRVQNFSSF EKTWIGLLKL VALSKTSAFN VDCITLKHVD FTYLDDEKFR ATIHDDNT F TLHFFNRHSP FHLISQFLQD TFSDGPSAIQ PLRVIMDRTR GVLVAQELGY VVLARSLRQY RIILSKNHGI QVLLNRHGC ILQDLSYLSA DSRYLEGTQT LTSQWEPCSW LNTVWEGDLG DDELNGQIEA APEMHLIKMN KTADLTAILK RILAISRKK (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

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Macromolecule #9: Mediator complex subunit 4

MacromoleculeName: Mediator complex subunit 4 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 20.35801 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

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Macromolecule #10: Mediator complex subunit 7

MacromoleculeName: Mediator complex subunit 7 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 43.573094 KDa
SequenceString: MEPNEGVQLF SAFPPPPPYY KLFTRENIEK VISNMEKEAK HEDDANTLQP KTEEEIESLA KLFKKPSCLT SGTYQMFGDT WRLDEAIPS LKEFGIPELY KDIKDGEDEI EVVEYDPKSN AIVGTSFTRV HDYDKNSPIE NEKILEDDQH TAMKEKDNES D KTMKDVEE ...String:
MEPNEGVQLF SAFPPPPPYY KLFTRENIEK VISNMEKEAK HEDDANTLQP KTEEEIESLA KLFKKPSCLT SGTYQMFGDT WRLDEAIPS LKEFGIPELY KDIKDGEDEI EVVEYDPKSN AIVGTSFTRV HDYDKNSPIE NEKILEDDQH TAMKEKDNES D KTMKDVEE KTEPSLKKEE EDIQMKEPLD SQDTGAVSAS SVNEGFRADQ KSKDGETSDL IKIPRRAYEL RFLSRSLMLN FL ELLGIMA KAPEQFPSKV ENIRVLLLNL HHLINDYRPH QSRESLIMLL EKQLKHEESQ VELLRTHNRQ MTETLEKYKS LDF NMEKEG DVIQQLKSSI KKPLSGAEDE QKSRSMFSKN DEKLKKSLEL MEDVIKRDLS

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Macromolecule #11: Mediator complex subunit 21

MacromoleculeName: Mediator complex subunit 21 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 15.821031 KDa
SequenceString:
MACRCTQLQD TIDEVATQFY SSIHYLSSHH DFVPLPGQEK VSDSKVNPIS AEELQFAQRD LAKDLVTKFM QIDTLINQLP GISTAPKHQ LEKIKKLQNS IEEKQLERKS LESENEDLKL QLAKRIETFG RLSCVLFQP

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Macromolecule #12: Mediator complex subunit 31

MacromoleculeName: Mediator complex subunit 31 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 16.913975 KDa
SequenceString:
METKWLLSKV PDDKSRFEIE LEFVQMLSNP WYLNFLAQHK YFEDEAFLQY LEYMEYWREP EYVKFIIYPT CLHMLTLLKN PQFRNDISR ADLSKQVNDE IYYEWLGKGL QQYGSADDAT LSQPQQEEDE KKVDVKKENE

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Macromolecule #13: Mediator complex subunit 27

MacromoleculeName: Mediator complex subunit 27 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 31.132693 KDa
SequenceString: MSLEEQRTRD ELKHKLLDLN QLHEQLAELR TICPSLLKLL HPETGTSRKF EKSAQEAIEK VNSFYTHLKS SQNVFDYAEK SLQADSSNL LPTYLYNSED LSNDTENNET KSINGKSALD LKEPHHSELH DNDNFQNSDI NIESFKGDIE ASGSILTTHE N KSFTLKLA ...String:
MSLEEQRTRD ELKHKLLDLN QLHEQLAELR TICPSLLKLL HPETGTSRKF EKSAQEAIEK VNSFYTHLKS SQNVFDYAEK SLQADSSNL LPTYLYNSED LSNDTENNET KSINGKSALD LKEPHHSELH DNDNFQNSDI NIESFKGDIE ASGSILTTHE N KSFTLKLA NELEFIFFHD TRGKFSVYCS SSKDDAITFS INRNNNFLGN LWSLMPKILD YQHLYSKPCD FCKSLISPVY LE LPSVRRN ANSTVKPTSK DILALHAECV PAQSDL

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Macromolecule #14: Mediator complex subunit 9

MacromoleculeName: Mediator complex subunit 9 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 13.810964 KDa
SequenceString:
MDPAHHIKDT SAELLSQQDF SILQSRLLEF LASQEARETV TASKELTLLR QGIRQLKEKV SKMEPEEMTV KEKKSIIEIL KARIALKKA FLKMALSESN DTVVKKEDVR ESPVDTFMEN AT

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Macromolecule #15: Mediator complex subunit 19

MacromoleculeName: Mediator complex subunit 19 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 11.847596 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #16: Mediator complex subunit 10

MacromoleculeName: Mediator complex subunit 10 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 11.251861 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)

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Macromolecule #17: RNA polymerase II subunit Rpb1

MacromoleculeName: RNA polymerase II subunit Rpb1 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 194.372625 KDa
SequenceString: MSGIQFSPSS VPLRRVEEVQ FGILSPEEIR SMSVAKIEFP ETMDESGQRP RVGGLLDPRL GTIDRQFKCQ TCGETMADCP GHFGHIELA KPVFHIGFLS KIKKILECVC WNCGKLKIDS SNPKFNDTQR YRDPKNRLNA VWNVCKTKMV CDTGLSAGSD N FDLSNPSA ...String:
MSGIQFSPSS VPLRRVEEVQ FGILSPEEIR SMSVAKIEFP ETMDESGQRP RVGGLLDPRL GTIDRQFKCQ TCGETMADCP GHFGHIELA KPVFHIGFLS KIKKILECVC WNCGKLKIDS SNPKFNDTQR YRDPKNRLNA VWNVCKTKMV CDTGLSAGSD N FDLSNPSA NMGHGGCGAA QPTIRKDGLR LWGSWKRGKD ESDLPEKRLL SPLEVHTIFT HISSEDLAHL GLNEQYARPD WM IITVLPV PPPSVRPSIS VDGTSRGEDD LTHKLSDIIK ANANVRRCEQ EGAPAHIVSE YEQLLQFHVA TYMDNEIAGQ PQA LQKSGR PLKSIRARLK GKEGRLRGNL MGKRVDFSAR TVITGDPNLS LDELGVPRSI AKTLTYPETV TPYNIYQLQE LVRN GPDEH PGAKYIIRDT GERIDLRYHK RAGDIPLRYG WRVERHIRDG DVVIFNRQPS LHKMSMMGHR IRVMPYSTFR LNLSV TSPY NADFDGDEMN MHVPQSEETR AEIQEITMVP KQIVSPQSNK PVMGIVQDTL AGVRKFSLRD NFLTRNAVMN IMLWVP DWD GILPPPVILK PKVLWTGKQI LSLIIPKGIN LIRDDDKQSL SNPTDSGMLI ENGEIIYGVV DKKTVGASQG GLVHTIW KE KGPEICKGFF NGIQRVVNYW LLHNGFSIGI GDTIADADTM KEVTRTVKEA RRQVAECIQD AQHNRLKPEP GMTLRESF E AKVSRILNQA RDNAGRSAEH SLKDSNNVKQ MVAAGSKGSF INISQMSACV GQQIVEGKRI PFGFKYRTLP HFPKDDDSP ESRGFIENSY LRGLTPQEFF FHAMAGREGL IDTAVKTAET GYIQRRLVKA MEDVMVRYDG TVRNAMGDII QFAYGEDGLD ATLVEYQVF DSLRLSTKQF EKKYRIDLME DRSLSLYMEN SIENDSSVQD LLDEEYTQLV ADRELLCKFI FPKGDARWPL P VNVQRIIQ NALQIFHLEA KKPTDLLPSD IINGLNELIA KLTIFRGSDR ITRDVQNNAT LLFQILLRSK FAVKRVIMEY RL NKVAFEW IMGEVEARFQ QAVVSPGEMV GTLAAQSIGE PATQMTLNTF HYAGVSSKNV TLGVPRLKEI LNVAKNIKTP SLT IYLMPW IAANMDLAKN VQTQIEHTTL STVTSATEIH YDPDPQDTVI EEDKDFVEAF FAIPDEEVEE NLYKQSPWLL RLEL DRAKM LDKKLSMSDV AGKIAESFER DLFTIWSEDN ADKLIIRCRI IRDDDRKAED DDNMIEEDVF LKTIEGHMLE SISLR GVPN ITRVYMMEHK IVRQIEDGTF ERADEWVLET DGINLTEAMT VEGVDATRTY SNSFVEILQI LGIEATRSAL LKELRN VIE FDGSYVNYRH LALLCDVMTS RGHLMAITRH GINRAETGAL MRCSFEETVE ILMDAAASGE KDDCKGISEN IMLGQLA PM GTGAFDIYLD QDMLMNYSLG TAVPTLAGSG MGTSQLPEGA GTPYERSPMV DSGFVGSPDA AAFSPLVQGG SEGREGFG D YGLLGAASPY KGVQSPGYTS PFSSAMSPGY GLTSPSYSPS SPGYSTSPAY MPSSPSYSPT SPSYSPTSPS YSPTSPSYS PTSPSYSATS PSYSPTSPSY SPTSPSYSPT SPSYSPTSPS YSPTSPSYSP TSPSYSPTSP SYSPTSPSYS PTSPSYSPTS PSYSPTSPS YSPTSPSYSP TSPSYSPTSP SYSPTSPSYS PTSPSYSPTS PSYSPTSPSY SPTSPSYSPT SPS

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Macromolecule #18: RNA polymerase II subunit Rpb2

MacromoleculeName: RNA polymerase II subunit Rpb2 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 138.027188 KDa
SequenceString: MSYEDYQYNE TLTQEDCWTV ISSFFEETSL ARQQLFSFDE FVQNTMQEIV DDDSTLTLDQ YAQHTGAQGD VTRRYEINFG QIYLSRPTM TEADGSTTTM FPQEARLRNL TYSSPLYVDM RKKVMVAADS NVPIGEEEWL VEEEDEEPSK VFIGKIPIML R STFCILNG ...String:
MSYEDYQYNE TLTQEDCWTV ISSFFEETSL ARQQLFSFDE FVQNTMQEIV DDDSTLTLDQ YAQHTGAQGD VTRRYEINFG QIYLSRPTM TEADGSTTTM FPQEARLRNL TYSSPLYVDM RKKVMVAADS NVPIGEEEWL VEEEDEEPSK VFIGKIPIML R STFCILNG VSDSELYDLN ECPYDQGGYF IINGSEKVII AQERSAANIV QVFKKAAPSP IAYVAEIRSA LERGSRLISS MQ IKLMARN TENSGQTIRA TLPYIRSDIP IVIVFRALGV VPDRDILEHI CYDPNDFQML EMMKPCIEEA FVIQDKDIAL DYI GKRGST TGVTREKRLR YAHDILQKEL LPHITTMEGF ETRKAFFLGY MIHRMLLCAL ERREPDDRDH FGKKRLDLAG PLLA SLFRM LFRKMTRDVY KYMQKCVETN REFNLTLAVK SNIITNGLRY SLATGNWGDQ KRSMVNRVGV SQVLNRYTFA STLSH LRRT NTPIGRDGKL AKPRQLHNTH WGMVCPAETP EGQACGLVKN LSLMSYVSVG SPSAPIIEFL EEWGLETLED YNPSAS PNA TKVFVNGVWL GVHRDPAHLT ETLRSLRRRL DISAEVSIVR DIREKELRLF TDAGRICRPL FIVDNNPNSE RRGELCI RK EHIQQLIEDK DRYDIDPEQR FGWTALVSSG LIEYLDAEEE ETVMIAMSPE DLEASRQMQA GYEVKEELDP AQRVKPAP N PHVHAWTHCE IHPAMILGIL ASIIPFPDHN QSPRNTYQSA MGKQAMGVYL TNYQVRMDTM ANILYYPQKP LATTRSMEY LKFRELPAGQ NAIVAILCYS GYNQEDSIIM NQASIDRGLF RSIFYRTYTD QEKKIGMTVM EEFERPVRST TLRMKHGTYD KLEDDGLIA PGTRVSGEDI IIGKTAPIPL DHEELGQRTQ LHAKRDVSTP LRSTESGIVD QVMVTTNQEG LKFVKVRMRS T RIPQIGDK FASRHGQKGT IGMTYRHEDM PFSAQGIVPD IIINPHAIPS RMTVAHLVEC QLSKVSALSG FEGDATPFTD VT VEAVSKL LRSHGFQSRG FEVMYHGHTG RKLVAQVFLG PTYYQRLKHL VDDKIHARAR GPVQILTRQP VEGRSRDGGL RFG EMERDC QISHGCSSVL RERLFDCSDA YRVIVCDICG LIAIASYKKD SYECRSCQNR TRFSQVYLPY AAKLLFQELM SMNI APRLF TKNHK

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Macromolecule #19: RNA polymerase II subunit Rpb3

MacromoleculeName: RNA polymerase II subunit Rpb3 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 33.748816 KDa
SequenceString: MDSETHITIR NISKNSVDFV LTNTSLAVAN SLRRVVLAEI PTVAIDLVEI NVNTSVMPDE FLAHRLGMIP LDSSNIDEPP PVGLEYTRN CDCDQYCPKC SVELFLNAKC TGEGTMEIYA RDLVVSSNSS LGHPILADPK SRGPLICKLR KEQEISLRCI A KKGIAKEH ...String:
MDSETHITIR NISKNSVDFV LTNTSLAVAN SLRRVVLAEI PTVAIDLVEI NVNTSVMPDE FLAHRLGMIP LDSSNIDEPP PVGLEYTRN CDCDQYCPKC SVELFLNAKC TGEGTMEIYA RDLVVSSNSS LGHPILADPK SRGPLICKLR KEQEISLRCI A KKGIAKEH AKWSPTSAVA FEYDPWNKLQ HTDYWFENDA DAEWPKSKNA DWEEPPREGE PFNFQEEPRR FYMDVESVGS IP PNEIMVQ GLRILQEKLA VLVRDLDEEQ PTQLSANELN MEENAEMNWS PYQNGEENTW

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Macromolecule #20: RNA polymerase II subunit Rpb4

MacromoleculeName: RNA polymerase II subunit Rpb4 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 15.37949 KDa
SequenceString:
MPRAIFEEDA AQLKLGPEFE NEDMLTVSEA KILIETVLAQ RARETNGEIP MTDVMKKTVA YFNVFARFKT AEATYACERI LGNRFHKFE RAQLGTLCCE DAEEARTLIP SLANKIDDQN LQGILDELST LRKFQD

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Macromolecule #21: RNA polymerase II subunit Rpb5

MacromoleculeName: RNA polymerase II subunit Rpb5 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 23.954504 KDa
SequenceString: MSAEEKNIVR VFRAWKTAHQ LVHDRGYGVS QAELDLTLDQ FKAMHCGMGR NLDRTTLSFY AKPSNDSNKG TIYIEFAKEP SVGIKEMRT FVHTLGDHNH KTGILIYANS MTPSAAKIIA TVTGQFTIET FQESDLIVNI THHELVPKHI LLSPDEKKEL L DRYKLRET ...String:
MSAEEKNIVR VFRAWKTAHQ LVHDRGYGVS QAELDLTLDQ FKAMHCGMGR NLDRTTLSFY AKPSNDSNKG TIYIEFAKEP SVGIKEMRT FVHTLGDHNH KTGILIYANS MTPSAAKIIA TVTGQFTIET FQESDLIVNI THHELVPKHI LLSPDEKKEL L DRYKLRET QLPRIQLADP VARYLGLKRG EVVKIVRRSE TSGRYNSYRI CA

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Macromolecule #22: RNA polymerase II subunit Rpb6

MacromoleculeName: RNA polymerase II subunit Rpb6 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 15.742497 KDa
SequenceString:
MSDYEEDEAF GMDGAVMEEE VDELEMIDEN GQSQQGVSHP GEPSTTVITE DVASSKTAQS GKAVAKEDRT TTPYMTKYER ARILGTRAL QISMNAPVLV DLEGETDPLQ IAMKELAQKK IPLLVRRYLP DGSYEDWSVA ELI

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Macromolecule #23: RNA polymerase II subunit Rpb7

MacromoleculeName: RNA polymerase II subunit Rpb7 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 19.121982 KDa
SequenceString:
MPFFLKELSL TISLHPSYFG PRMQDYLKAK LLADVEGTCS GQYGYIICVL DSNTIDIDKG RVVPGQGFAE FEVKYRAVLW RPFRGEVVD AIVTTVNKMG FFANIGPLNV FVSSHLVPPD MKFDPTANPP NYSGEDQVIE KGSNVRLKIV GTRTDATEIF A IATMKEDY LGVL

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Macromolecule #24: RNA polymerase II subunit Rpb8

MacromoleculeName: RNA polymerase II subunit Rpb8 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 14.317318 KDa
SequenceString:
MSESVLLDEI FTVTSVDKQK YQRVSRITAV SGQNDMNLTL DINSQIYPLE KDATFSLQIT SNLNSPDLKE AADYIMYGKV YRVEEAKDE KVSVYVSFGG LLMAIEGSHR KLYRLSLDHV YLLLRR

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Macromolecule #25: RNA polymerase II subunit Rpb9

MacromoleculeName: RNA polymerase II subunit Rpb9 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 13.192771 KDa
SequenceString:
MSNFQYCIEC NNMLYPREDK VDRVLRLACR NCDYSEIAAT SKVYRHELQS SNVENTTVSH DASTDPTLPR SDKECPRCHQ HEAVFYQTH SRRGDTMMTL IYVCVHCGFA FEEQ

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Macromolecule #26: RNA polymerase II subunit Rpb10

MacromoleculeName: RNA polymerase II subunit Rpb10 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 8.286801 KDa
SequenceString:
MIIPIRCFSC GKVIGDKWDT YLTLLQEDNT EGEALDKLGL QRYCCRRMIL THVDLIEKLL CYNPLSKQKN L

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Macromolecule #27: RNA polymerase II subunit Rpb11

MacromoleculeName: RNA polymerase II subunit Rpb11 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 14.143276 KDa
SequenceString:
MNQPERYELI ELMGLPKVTY ELDSKSPNAA VVTLEKEDHT LANMLANQLL SDERVLFAGY KVPHPLNHNF ILRVQTVEDC SPKQVIVDA AKSLITHLEE IKVNFMREWE LKMISVEGVE MEFS

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Macromolecule #28: RNA polymerase II subunit Rpb12

MacromoleculeName: RNA polymerase II subunit Rpb12 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Fission yeast (fission yeast)
Molecular weightTheoretical: 7.216495 KDa
SequenceString:
MNHPTSTGGT AFNPPRPATM IYLCADCGAR NTIQAKEVIR CRECGHRVMY KMRTKRMVQF EAR

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1 mg/mL
BufferpH: 7.4
Details: 20 mM HEPES, pH 7.4, 200 mM potassium acetate, 5 mM b-Mercaptoethanol, 0.01% NP-40
GridModel: C-flat-2/2 4C / Material: COPPER / Mesh: 400
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Instrument: HOMEMADE PLUNGER

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 100.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm / Nominal defocus max: 4.0 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 22500
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average exposure time: 7.0 sec. / Average electron dose: 9.6 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionSoftware - Name: CTFFIND (ver. 3)
Initial angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 1.4)
Final angle assignmentType: ANGULAR RECONSTITUTION / Software - Name: RELION (ver. 1.4)
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 7.8 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 1.4) / Number images used: 3862
FSC plot (resolution estimation)

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About Yorodumi

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