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- PDB-7sc6: tRNA-like Structure from Brome Mosaic Virus Bound to Tyrosyl-tRNA... -

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Basic information

Entry
Database: PDB / ID: 7sc6
TitletRNA-like Structure from Brome Mosaic Virus Bound to Tyrosyl-tRNA Synthetase from Phaseolus vulgaris. Conformation: Bound State 1.
Components
  • Tyrosine--tRNA ligase
  • tRNA-like structure from brome mosaic virus RNA 3
KeywordsRNA BINDING PROTEIN/RNA / tRNA-synthetase / Viral RNA / 3' UTR / RNA BINDING PROTEIN / RNA BINDING PROTEIN-RNA complex
Function / homology
Function and homology information


tyrosine-tRNA ligase / tyrosine-tRNA ligase activity / tRNA aminoacylation for protein translation / ATP binding
Similarity search - Function
Tyrosine-tRNA ligase, archaeal/eukaryotic-type / Aminoacyl-tRNA synthetase, class Ic / tRNA synthetases class I (W and Y) / Rossmann-like alpha/beta/alpha sandwich fold
Similarity search - Domain/homology
: / RNA / RNA (> 10) / RNA (> 100) / Tyrosine--tRNA ligase
Similarity search - Component
Biological speciesPhaseolus vulgaris (French bean)
Brome mosaic virus
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 5.51 Å
AuthorsKieft, J.S. / Bonilla, S.L.
Funding support United States, 3items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM118070 United States
National Science Foundation (NSF, United States)F32GM139385 United States
Howard Hughes Medical Institute (HHMI)Hanna H. Gray Fellowship United States
CitationJournal: Science / Year: 2021
Title: A viral RNA hijacks host machinery using dynamic conformational changes of a tRNA-like structure.
Authors: Steve L Bonilla / Madeline E Sherlock / Andrea MacFadden / Jeffrey S Kieft /
Abstract: Viruses require multifunctional structured RNAs to hijack their host’s biochemistry, but their mechanisms can be obscured by the difficulty of solving conformationally dynamic RNA structures. Using ...Viruses require multifunctional structured RNAs to hijack their host’s biochemistry, but their mechanisms can be obscured by the difficulty of solving conformationally dynamic RNA structures. Using cryo–electron microscopy (cryo-EM), we visualized the structure of the mysterious viral transfer RNA (tRNA)–like structure (TLS) from the brome mosaic virus, which affects replication, translation, and genome encapsidation. Structures in isolation and those bound to tyrosyl-tRNA synthetase (TyrRS) show that this ~55-kilodalton purported tRNA mimic undergoes large conformational rearrangements to bind TyrRS in a form that differs substantially from that of tRNA. Our study reveals how viral RNAs can use a combination of static and dynamic RNA structures to bind host machinery through highly noncanonical interactions, and we highlight the utility of cryo-EM for visualizing small, conformationally dynamic structured RNAs.
History
DepositionSep 27, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 1, 2021Provider: repository / Type: Initial release

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Structure visualization

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Assembly

Deposited unit
A: Tyrosine--tRNA ligase
B: Tyrosine--tRNA ligase
C: tRNA-like structure from brome mosaic virus RNA 3


Theoretical massNumber of molelcules
Total (without water)146,0093
Polymers146,0093
Non-polymers00
Water0
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, 2D classes displayed 2 RNAs bound to tyrosyl-synthetase dimer. One of the RNAs was not well defined. Only one RNA structure was modelled.
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Protein Tyrosine--tRNA ligase


Mass: 45482.191 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Phaseolus vulgaris (French bean) / Gene: PHAVU_002G027700g / Production host: Escherichia coli (E. coli) / References: UniProt: V7CJ18, tyrosine-tRNA ligase
#2: RNA chain tRNA-like structure from brome mosaic virus RNA 3


Mass: 55044.559 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Brome mosaic virus / References: GenBank: 556693

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: RNA-protein complex: tyrosyl-tRNA synthetase from Phaseolus vulgaris bound to tRNA-like structure from brome mosaic virus RNA 3.
Type: COMPLEX / Entity ID: all / Source: RECOMBINANT
Source (natural)Organism: Phaseolus vulgaris (French bean)
Source (recombinant)Organism: Escherichia coli (E. coli)
Buffer solutionpH: 7
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid material: COPPER / Grid mesh size: 400 divisions/in. / Grid type: C-flat-1.2/1.3
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277 K / Details: Blot for 2.5 seconds before plunging.

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: OTHER
Electron lensMode: BRIGHT FIELDBright-field microscopy / Cs: 2.7 mm
Image recordingElectron dose: 60 e/Å2 / Film or detector model: FEI FALCON III (4k x 4k)
EM imaging opticsEnergyfilter name: GIF Bioquantum

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Processing

SoftwareName: PHENIX / Version: 1.19.2_4158: / Classification: refinement
EM softwareName: cryoSPARC / Category: image acquisition
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 5.51 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 129643 / Symmetry type: POINT
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.0039738
ELECTRON MICROSCOPYf_angle_d0.58513953
ELECTRON MICROSCOPYf_dihedral_angle_d14.2362776
ELECTRON MICROSCOPYf_chiral_restr0.0341674
ELECTRON MICROSCOPYf_plane_restr0.0051157

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