登録情報 データベース : PDB / ID : 7mjq 構造の表示 ダウンロードとリンクタイトル Vascular KATP channel: Kir6.1 SUR2B quatrefoil-like conformation 2 要素ATP-sensitive inward rectifier potassium channel 8 Isoform SUR2B of ATP-binding cassette sub-family C member 9 詳細キーワード TRANSPORT PROTEIN / KATP / potassium channel / vascular機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
cardiac pacemaker cell differentiation / atrioventricular node cell differentiation / substrate-dependent cell migration, cell contraction / reactive gliosis / ATP sensitive Potassium channels / membrane repolarization during ventricular cardiac muscle cell action potential / ABC-family proteins mediated transport / response to resveratrol / ATP-activated inward rectifier potassium channel activity / glutamate secretion, neurotransmission ... cardiac pacemaker cell differentiation / atrioventricular node cell differentiation / substrate-dependent cell migration, cell contraction / reactive gliosis / ATP sensitive Potassium channels / membrane repolarization during ventricular cardiac muscle cell action potential / ABC-family proteins mediated transport / response to resveratrol / ATP-activated inward rectifier potassium channel activity / glutamate secretion, neurotransmission / circulatory system development / inward rectifying potassium channel / NLRP3 inflammasome complex assembly / sulfonylurea receptor activity / voltage-gated potassium channel activity involved in ventricular cardiac muscle cell action potential repolarization / ventricular cardiac muscle tissue development / CAMKK-AMPK signaling cascade / cardiac conduction / voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential / coronary vasculature development / ATPase-coupled monoatomic cation transmembrane transporter activity / cardiac muscle cell contraction / vasculature development / synaptic assembly at neuromuscular junction / regulation of monoatomic ion transmembrane transport / inorganic cation transmembrane transport / nervous system process / syntaxin binding / sulfonylurea receptor binding / neuromuscular process / action potential / establishment of cell polarity / response to ATP / Ion homeostasis / myofibril / heterocyclic compound binding / response to exogenous dsRNA / fat cell differentiation / blood vessel development / transmission of nerve impulse / monoatomic cation transmembrane transport / p38MAPK cascade / potassium ion import across plasma membrane / protein secretion / ATPase-coupled transmembrane transporter activity / potassium channel regulator activity / fatty acid transport / ABC-type transporter activity / heart morphogenesis / presynaptic active zone membrane / potassium ion transmembrane transport / T-tubule / negative regulation of blood pressure / regulation of heart rate / response to endoplasmic reticulum stress / sarcomere / acrosomal vesicle / response to cytokine / kidney development / response to ischemia / determination of adult lifespan / calcium ion transmembrane transport / microglial cell activation / potassium ion transport / response to insulin / transmembrane transport / sarcolemma / regulation of blood pressure / vasodilation / presynapse / heart development / gene expression / fibroblast proliferation / defense response to virus / adaptive immune response / cell population proliferation / transmembrane transporter binding / response to lipopolysaccharide / response to hypoxia / response to xenobiotic stimulus / inflammatory response / glutamatergic synapse / apoptotic process / ATP hydrolysis activity / protein-containing complex / ATP binding / identical protein binding / membrane / plasma membrane 類似検索 - 分子機能 Potassium channel, inwardly rectifying, Kir6.1 / ATP-binding cassette subfamily C member 9 / : / Sulphonylurea receptor / Potassium channel, inwardly rectifying, transmembrane domain / Inward rectifier potassium channel transmembrane domain / Potassium channel, inwardly rectifying, Kir, cytoplasmic / Potassium channel, inwardly rectifying, Kir / Inward rectifier potassium channel, C-terminal / Inward rectifier potassium channel C-terminal domain ... Potassium channel, inwardly rectifying, Kir6.1 / ATP-binding cassette subfamily C member 9 / : / Sulphonylurea receptor / Potassium channel, inwardly rectifying, transmembrane domain / Inward rectifier potassium channel transmembrane domain / Potassium channel, inwardly rectifying, Kir, cytoplasmic / Potassium channel, inwardly rectifying, Kir / Inward rectifier potassium channel, C-terminal / Inward rectifier potassium channel C-terminal domain / ABC transporter transmembrane region / ABC transporter type 1, transmembrane domain / ABC transporter integral membrane type-1 fused domain profile. / ABC transporter type 1, transmembrane domain superfamily / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter / ABC transporter-like, ATP-binding domain / ATP-binding cassette, ABC transporter-type domain profile. / Immunoglobulin E-set / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase 類似検索 - ドメイン・相同性 ADENOSINE-5'-TRIPHOSPHATE / : / ATP-binding cassette sub-family C member 9 / ATP-sensitive inward rectifier potassium channel 8 類似検索 - 構成要素生物種 Rattus norvegicus (ドブネズミ)手法 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 4.2 Å 詳細データ登録者 Sung, M.W. / Shyng, S.L. 資金援助 米国, 1件 詳細 詳細を隠す組織 認可番号 国 National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK) R01DK066485-13 米国
引用ジャーナル : Proc Natl Acad Sci U S A / 年 : 2021タイトル : Vascular K channel structural dynamics reveal regulatory mechanism by Mg-nucleotides.著者 : Min Woo Sung / Zhongying Yang / Camden M Driggers / Bruce L Patton / Barmak Mostofian / John D Russo / Daniel M Zuckerman / Show-Ling Shyng / 要旨 : Vascular tone is dependent on smooth muscle K channels comprising pore-forming Kir6.1 and regulatory SUR2B subunits, in which mutations cause Cantú syndrome. Unique among K isoforms, they lack ... Vascular tone is dependent on smooth muscle K channels comprising pore-forming Kir6.1 and regulatory SUR2B subunits, in which mutations cause Cantú syndrome. Unique among K isoforms, they lack spontaneous activity and require Mg-nucleotides for activation. Structural mechanisms underlying these properties are unknown. Here, we determined cryogenic electron microscopy structures of vascular K channels bound to inhibitory ATP and glibenclamide, which differ informatively from similarly determined pancreatic K channel isoform (Kir6.2/SUR1). Unlike SUR1, SUR2B subunits adopt distinct rotational "propeller" and "quatrefoil" geometries surrounding their Kir6.1 core. The glutamate/aspartate-rich linker connecting the two halves of the SUR-ABC core is observed in a quatrefoil-like conformation. Molecular dynamics simulations reveal MgADP-dependent dynamic tripartite interactions between this linker, SUR2B, and Kir6.1. The structures captured implicate a progression of intermediate states between MgADP-free inactivated, and MgADP-bound activated conformations wherein the glutamate/aspartate-rich linker participates as mobile autoinhibitory domain, suggesting a conformational pathway toward K channel activation. 履歴 登録 2021年4月20日 登録サイト : RCSB / 処理サイト : RCSB改定 1.0 2021年10月13日 Provider : repository / タイプ : Initial release改定 1.1 2022年10月26日 Group : Database references / カテゴリ : citation / citation_authorItem : _citation.country / _citation.journal_abbrev ... _citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
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