presynaptic endocytosis / regulation of clathrin-dependent endocytosis / clathrin complex / AP-2 adaptor complex binding / Cargo recognition for clathrin-mediated endocytosis / Clathrin-mediated endocytosis / Notch binding / clathrin-coated vesicle / positive regulation of Notch signaling pathway / cell leading edge ...presynaptic endocytosis / regulation of clathrin-dependent endocytosis / clathrin complex / AP-2 adaptor complex binding / Cargo recognition for clathrin-mediated endocytosis / Clathrin-mediated endocytosis / Notch binding / clathrin-coated vesicle / positive regulation of Notch signaling pathway / cell leading edge / calyx of Held / clathrin-coated pit / terminal bouton / regulation of protein localization / presynapse / protein stabilization / non-specific serine/threonine protein kinase / protein phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / ATP binding / plasma membrane / cytosol 類似検索 - 分子機能
Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily 類似検索 - ドメイン・相同性
Chem-5P6 / AP2-associated protein kinase 1 類似検索 - 構成要素
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 1 Å / 相対比: 1
反射
解像度: 2.12→58.44 Å / Num. obs: 43896 / % possible obs: 92.7 % / 冗長度: 9.6 % / Biso Wilson estimate: 58.46 Å2 / Rmerge(I) obs: 0.058 / Net I/σ(I): 18.4
反射 シェル
解像度: 2.12→2.21 Å / 冗長度: 10.5 % / Rmerge(I) obs: 1.387 / Mean I/σ(I) obs: 1.5 / Num. unique obs: 2196 / % possible all: 48.7
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解析
ソフトウェア
名称
バージョン
分類
BUSTER
精密化
SCALA
3.3.16
データスケーリング
PDB_EXTRACT
3.27
データ抽出
XDS
データ削減
PHASER
位相決定
精密化
構造決定の手法: 分子置換 開始モデル: In house model 解像度: 2.124→21.24 Å / Cor.coef. Fo:Fc: 0.948 / Cor.coef. Fo:Fc free: 0.949 / SU R Cruickshank DPI: 0.459 / 交差検証法: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.232 / SU Rfree Blow DPI: 0.178 / SU Rfree Cruickshank DPI: 0.177 詳細: HYDROGENS WERE FULLY REFINED WITH ZERO OCCUPANCY AT NUCLEAR POSITION. REFINEMENT NOTES. NUMBER OF REFINEMENT NOTES : 1 NOTE 1 : IDEAL-DIST CONTACT TERM CONTACT SETUP. ALL ATOMS HAVE CCP4 ATOM TYPE FROM LIBRARY