Natural Sciences and Engineering Research Council (NSERC, Canada)
カナダ
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
S10 OD018142-01
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
S10 RR025080-01
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01 GM109524
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
R01 GM082946
米国
引用
ジャーナル: Proc Natl Acad Sci U S A / 年: 2020 タイトル: Molecular biology and structure of a novel penaeid shrimp densovirus elucidate convergent parvoviral host capsid evolution. 著者: Judit J Pénzes / Hanh T Pham / Paul Chipman / Nilakshee Bhattacharya / Robert McKenna / Mavis Agbandje-McKenna / Peter Tijssen / 要旨: The giant tiger prawn () is a decapod crustacean widely reared for human consumption. Currently, viruses of two distinct lineages of parvoviruses (PVs, family ; subfamily ) infect penaeid shrimp. ...The giant tiger prawn () is a decapod crustacean widely reared for human consumption. Currently, viruses of two distinct lineages of parvoviruses (PVs, family ; subfamily ) infect penaeid shrimp. Here, a PV was isolated and cloned from Vietnamese specimens, designated metallodensovirus (PmMDV). This is the first member of a third divergent lineage shown to infect penaeid decapods. PmMDV has a transcription strategy unique among invertebrate PVs, using extensive alternative splicing and incorporating transcription elements characteristic of vertebrate-infecting PVs. The PmMDV proteins have no significant sequence similarity with other PVs, except for an SF3 helicase domain in its nonstructural protein. Its capsid structure, determined by cryoelectron microscopy to 3-Å resolution, has a similar surface morphology to densovirus, despite the lack of significant capsid viral protein (VP) sequence similarity. Unlike other PVs, PmMDV folds its VP without incorporating a βA strand and displayed unique multimer interactions, including the incorporation of a Ca cation, attaching the N termini under the icosahedral fivefold symmetry axis, and forming a basket-like pentamer helix bundle. While the PmMDV VP sequence lacks a canonical phospholipase A2 domain, the structure of an EDTA-treated capsid, determined to 2.8-Å resolution, suggests an alternative membrane-penetrating cation-dependent mechanism in its N-terminal region. PmMDV is an observed example of convergent evolution among invertebrate PVs with respect to host-driven capsid structure and unique as a PV showing a cation-sensitive/dependent basket structure for an alternative endosomal egress.
A: Penaeus monodon metallodensovirus major capsid protein B: Penaeus monodon metallodensovirus major capsid protein C: Penaeus monodon metallodensovirus major capsid protein D: Penaeus monodon metallodensovirus major capsid protein E: Penaeus monodon metallodensovirus major capsid protein F: Penaeus monodon metallodensovirus major capsid protein G: Penaeus monodon metallodensovirus major capsid protein H: Penaeus monodon metallodensovirus major capsid protein I: Penaeus monodon metallodensovirus major capsid protein J: Penaeus monodon metallodensovirus major capsid protein K: Penaeus monodon metallodensovirus major capsid protein L: Penaeus monodon metallodensovirus major capsid protein M: Penaeus monodon metallodensovirus major capsid protein N: Penaeus monodon metallodensovirus major capsid protein O: Penaeus monodon metallodensovirus major capsid protein P: Penaeus monodon metallodensovirus major capsid protein Q: Penaeus monodon metallodensovirus major capsid protein R: Penaeus monodon metallodensovirus major capsid protein S: Penaeus monodon metallodensovirus major capsid protein T: Penaeus monodon metallodensovirus major capsid protein U: Penaeus monodon metallodensovirus major capsid protein V: Penaeus monodon metallodensovirus major capsid protein W: Penaeus monodon metallodensovirus major capsid protein X: Penaeus monodon metallodensovirus major capsid protein Y: Penaeus monodon metallodensovirus major capsid protein Z: Penaeus monodon metallodensovirus major capsid protein 1: Penaeus monodon metallodensovirus major capsid protein 2: Penaeus monodon metallodensovirus major capsid protein 3: Penaeus monodon metallodensovirus major capsid protein 4: Penaeus monodon metallodensovirus major capsid protein 5: Penaeus monodon metallodensovirus major capsid protein 6: Penaeus monodon metallodensovirus major capsid protein a: Penaeus monodon metallodensovirus major capsid protein b: Penaeus monodon metallodensovirus major capsid protein c: Penaeus monodon metallodensovirus major capsid protein d: Penaeus monodon metallodensovirus major capsid protein e: Penaeus monodon metallodensovirus major capsid protein f: Penaeus monodon metallodensovirus major capsid protein g: Penaeus monodon metallodensovirus major capsid protein h: Penaeus monodon metallodensovirus major capsid protein i: Penaeus monodon metallodensovirus major capsid protein j: Penaeus monodon metallodensovirus major capsid protein k: Penaeus monodon metallodensovirus major capsid protein l: Penaeus monodon metallodensovirus major capsid protein m: Penaeus monodon metallodensovirus major capsid protein n: Penaeus monodon metallodensovirus major capsid protein o: Penaeus monodon metallodensovirus major capsid protein p: Penaeus monodon metallodensovirus major capsid protein q: Penaeus monodon metallodensovirus major capsid protein r: Penaeus monodon metallodensovirus major capsid protein s: Penaeus monodon metallodensovirus major capsid protein t: Penaeus monodon metallodensovirus major capsid protein u: Penaeus monodon metallodensovirus major capsid protein v: Penaeus monodon metallodensovirus major capsid protein w: Penaeus monodon metallodensovirus major capsid protein x: Penaeus monodon metallodensovirus major capsid protein y: Penaeus monodon metallodensovirus major capsid protein z: Penaeus monodon metallodensovirus major capsid protein 7: Penaeus monodon metallodensovirus major capsid protein 8: Penaeus monodon metallodensovirus major capsid protein