+Open data
-Basic information
Entry | Database: PDB / ID: 6w8o | ||||||
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Title | Structure of an Apo membrane protein | ||||||
Components | Endosomal/lysosomal potassium channel TMEM175 | ||||||
Keywords | MEMBRANE PROTEIN / Channel | ||||||
Function / homology | Function and homology information lysosomal lumen pH elevation / phagosome-lysosome fusion / regulation of lysosomal lumen pH / potassium ion leak channel activity / proton channel activity / arachidonate binding / potassium channel activity / potassium ion transmembrane transport / proton transmembrane transport / neuron cellular homeostasis ...lysosomal lumen pH elevation / phagosome-lysosome fusion / regulation of lysosomal lumen pH / potassium ion leak channel activity / proton channel activity / arachidonate binding / potassium channel activity / potassium ion transmembrane transport / proton transmembrane transport / neuron cellular homeostasis / lysosome / endosome membrane / endosome / lysosomal membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.4 Å | ||||||
Authors | Shen, C. / Fu, T.M. / Wang, L.F. / Rawson, S. / Wu, H. | ||||||
Citation | Journal: To Be Published Title: Structure of an Apo membrane protein Authors: Shen, C. / Fu, T.M. / Wang, L.F. / Rawson, S. / Xia, S.Y. / Wu, H. | ||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | Molecule: MolmilJmol/JSmol |
-Downloads & links
-Download
PDBx/mmCIF format | 6w8o.cif.gz | 144.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6w8o.ent.gz | 118.6 KB | Display | PDB format |
PDBx/mmJSON format | 6w8o.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6w8o_validation.pdf.gz | 781 KB | Display | wwPDB validaton report |
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Full document | 6w8o_full_validation.pdf.gz | 805.5 KB | Display | |
Data in XML | 6w8o_validation.xml.gz | 30.3 KB | Display | |
Data in CIF | 6w8o_validation.cif.gz | 44.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/w8/6w8o ftp://data.pdbj.org/pub/pdb/validation_reports/w8/6w8o | HTTPS FTP |
-Related structure data
Related structure data | 21576MC 6w8nC 6w8pC C: citing same article (ref.) M: map data used to model this data |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 55667.219 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: TMEM175 / Production host: Homo sapiens (human) / References: UniProt: Q9BSA9 |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Dimer of an Apo membrane protein / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Source (natural) | Organism: Homo sapiens (human) |
Source (recombinant) | Organism: Homo sapiens (human) |
Buffer solution | pH: 7.4 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD |
Image recording | Electron dose: 65.02 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
Software | Name: PHENIX / Version: 1.17.1_3660: / Classification: refinement | ||||||||||||||||||||||||
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CTF correction | Type: NONE | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 145051 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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