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- PDB-6k9k: Monomeric human ATM (Ataxia telangiectasia mutated) kinase -

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Basic information

Entry
Database: PDB / ID: 6k9k
TitleMonomeric human ATM (Ataxia telangiectasia mutated) kinase
ComponentsSerine-protein kinase ATM
KeywordsCELL CYCLE / DSB / Kinease / activity
Function / homology
Function and homology information


establishment of RNA localization to telomere / positive regulation of telomerase catalytic core complex assembly / cellular response to nitrosative stress / negative regulation of telomere capping / establishment of protein-containing complex localization to telomere / Sensing of DNA Double Strand Breaks / peptidyl-serine autophosphorylation / positive regulation of telomere maintenance via telomere lengthening / meiotic telomere clustering / pre-B cell allelic exclusion ...establishment of RNA localization to telomere / positive regulation of telomerase catalytic core complex assembly / cellular response to nitrosative stress / negative regulation of telomere capping / establishment of protein-containing complex localization to telomere / Sensing of DNA Double Strand Breaks / peptidyl-serine autophosphorylation / positive regulation of telomere maintenance via telomere lengthening / meiotic telomere clustering / pre-B cell allelic exclusion / DNA-dependent protein kinase activity / extrinsic component of synaptic vesicle membrane / male meiotic nuclear division / histone H2AXS139 kinase activity / histone mRNA catabolic process / regulation of telomere maintenance via telomerase / female meiotic nuclear division / lipoprotein catabolic process / DNA double-strand break processing / regulation of autophagosome assembly / cellular response to X-ray / V(D)J recombination / oocyte development / pexophagy / Impaired BRCA2 binding to PALB2 / DNA repair complex / reciprocal meiotic recombination / positive regulation of DNA damage response, signal transduction by p53 class mediator / Homologous DNA Pairing and Strand Exchange / Defective homologous recombination repair (HRR) due to BRCA1 loss of function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA1 binding function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA2/RAD51/RAD51C binding function / Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA) / 1-phosphatidylinositol-3-kinase activity / Resolution of D-loop Structures through Holliday Junction Intermediates / HDR through Single Strand Annealing (SSA) / response to ionizing radiation / negative regulation of B cell proliferation / cellular response to stress / TP53 Regulates Transcription of Caspase Activators and Caspases / mitotic spindle assembly checkpoint signaling / positive regulation of double-strand break repair / Impaired BRCA2 binding to RAD51 / mitotic G2 DNA damage checkpoint signaling / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / peroxisomal matrix / Presynaptic phase of homologous DNA pairing and strand exchange / replicative senescence / signal transduction in response to DNA damage / Regulation of HSF1-mediated heat shock response / somitogenesis / ovarian follicle development / regulation of cellular response to heat / cellular response to retinoic acid / positive regulation of telomere maintenance via telomerase / negative regulation of TORC1 signaling / positive regulation of cell adhesion / telomere maintenance / Pexophagy / DNA damage checkpoint signaling / thymus development / regulation of signal transduction by p53 class mediator / determination of adult lifespan / post-embryonic development / cellular response to reactive oxygen species / TP53 Regulates Transcription of DNA Repair Genes / DNA damage response, signal transduction by p53 class mediator / Nonhomologous End-Joining (NHEJ) / Stabilization of p53 / Autodegradation of the E3 ubiquitin ligase COP1 / cellular response to gamma radiation / double-strand break repair via homologous recombination / G2/M DNA damage checkpoint / brain development / Regulation of TP53 Activity through Methylation / double-strand break repair via nonhomologous end joining / DNA Damage/Telomere Stress Induced Senescence / HDR through Homologous Recombination (HRR) / Meiotic recombination / multicellular organism growth / spindle / intrinsic apoptotic signaling pathway in response to DNA damage / cellular senescence / Regulation of TP53 Degradation / double-strand break repair / positive regulation of neuron apoptotic process / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / site of double-strand break / heart development / chromosome / protein autophosphorylation / Processing of DNA double-strand break ends / neuron apoptotic process / regulation of apoptotic process / Regulation of TP53 Activity through Phosphorylation / protein phosphorylation / non-specific serine/threonine protein kinase / regulation of cell cycle / regulation of autophagy / positive regulation of cell migration
Similarity search - Function
Telomere-length maintenance and DNA damage repair / Serine/threonine-protein kinase ATM, plant / ATM, catalytic domain / Telomere-length maintenance and DNA damage repair / Telomere-length maintenance and DNA damage repair / FATC domain / PIK-related kinase, FAT / FAT domain / FATC / FATC domain ...Telomere-length maintenance and DNA damage repair / Serine/threonine-protein kinase ATM, plant / ATM, catalytic domain / Telomere-length maintenance and DNA damage repair / Telomere-length maintenance and DNA damage repair / FATC domain / PIK-related kinase, FAT / FAT domain / FATC / FATC domain / PIK-related kinase / FAT domain profile. / FATC domain profile. / Phosphatidylinositol 3- and 4-kinases signature 1. / Phosphatidylinositol 3/4-kinase, conserved site / Phosphatidylinositol 3- and 4-kinases signature 2. / Phosphatidylinositol 3-/4-kinase, catalytic domain superfamily / Phosphoinositide 3-kinase, catalytic domain / Phosphatidylinositol 3- and 4-kinase / Phosphatidylinositol 3- and 4-kinases catalytic domain profile. / Phosphatidylinositol 3-/4-kinase, catalytic domain / Armadillo-type fold / Protein kinase-like domain superfamily
Similarity search - Domain/homology
Serine-protein kinase ATM
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 7.82 Å
AuthorsXiao, J. / Liu, M. / Qi, Y. / Yuriy, C. / Zhang, P. / Xu, Y.
CitationJournal: Cell Res / Year: 2019
Title: Structural insights into the activation of ATM kinase.
Authors: Jianxiong Xiao / Mengjie Liu / Yilun Qi / Yuriy Chaban / Chao Gao / Beiqing Pan / Yuan Tian / Zishuo Yu / Jie Li / Peijun Zhang / Yanhui Xu /
History
DepositionJun 16, 2019Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Dec 25, 2019Provider: repository / Type: Initial release
Revision 1.1Mar 27, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

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Assembly

Deposited unit
A: Serine-protein kinase ATM


Theoretical massNumber of molelcules
Total (without water)351,1281
Polymers351,1281
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: microscopy, gel filtration, equilibrium centrifugation
TypeNameSymmetry operationNumber
identity operation1_5551
Buried area0 Å2
ΔGint0 kcal/mol
Surface area144110 Å2

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Components

#1: Protein Serine-protein kinase ATM / Ataxia telangiectasia mutated / A-T mutated


Mass: 351127.688 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line: 293f / Gene: ATM / Cell line (production host): 293F / Production host: Homo sapiens (human)
References: UniProt: Q13315, non-specific serine/threonine protein kinase

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: monomeric human ATM (Ataxia telangiectasia mutated) kinase
Type: ORGANELLE OR CELLULAR COMPONENT / Entity ID: all / Source: RECOMBINANT
Molecular weightUnits: KILODALTONS/NANOMETER / Experimental value: NO
Source (natural)Organism: Homo sapiens (human)
Source (recombinant)Organism: Homo sapiens (human) / Cell: 293F
Buffer solutionpH: 7.4
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: OTHER
Electron lensMode: BRIGHT FIELD
Image recordingElectron dose: 32 e/Å2 / Film or detector model: FEI FALCON III (4k x 4k)

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Processing

Software
NameVersionClassification
phenix.real_space_refine1.15.2_3472refinement
PHENIX1.15.2_3472refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 7.82 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 53465 / Symmetry type: POINT
RefinementStereochemistry target values: GeoStd + Monomer Library + CDL v1.2
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.001316671
ELECTRON MICROSCOPYf_angle_d0.42222734
ELECTRON MICROSCOPYf_chiral_restr0.0362811
ELECTRON MICROSCOPYf_plane_restr0.00263011
ELECTRON MICROSCOPYf_dihedral_angle_d6.995510075

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