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- PDB-6k7v: Structure of NLRP1 CARD filament -

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Basic information

Entry
Database: PDB / ID: 6k7v
TitleStructure of NLRP1 CARD filament
ComponentsNACHT, LRR and PYD domains-containing protein 1
KeywordsSIGNALING PROTEIN / filament
Function / homology
Function and homology information


NLRP1 inflammasome complex assembly / NLRP1 inflammasome complex / The NLRP1 inflammasome / self proteolysis / NLRP3 inflammasome complex / cysteine-type endopeptidase activator activity / Hydrolases; Acting on peptide bonds (peptidases) / pattern recognition receptor signaling pathway / cysteine-type endopeptidase activator activity involved in apoptotic process / cellular response to UV-B ...NLRP1 inflammasome complex assembly / NLRP1 inflammasome complex / The NLRP1 inflammasome / self proteolysis / NLRP3 inflammasome complex / cysteine-type endopeptidase activator activity / Hydrolases; Acting on peptide bonds (peptidases) / pattern recognition receptor signaling pathway / cysteine-type endopeptidase activator activity involved in apoptotic process / cellular response to UV-B / pattern recognition receptor activity / pyroptotic inflammatory response / response to muramyl dipeptide / signaling adaptor activity / antiviral innate immune response / intrinsic apoptotic signaling pathway / activation of innate immune response / positive regulation of interleukin-1 beta production / molecular condensate scaffold activity / protein homooligomerization / Hydrolases; Acting on acid anhydrides; Acting on acid anhydrides to facilitate cellular and subcellular movement / positive regulation of inflammatory response / peptidase activity / double-stranded RNA binding / regulation of inflammatory response / double-stranded DNA binding / neuron apoptotic process / regulation of apoptotic process / defense response to virus / defense response to bacterium / inflammatory response / protein domain specific binding / apoptotic process / nucleolus / enzyme binding / signal transduction / ATP hydrolysis activity / nucleoplasm / ATP binding / nucleus / cytosol / cytoplasm
Similarity search - Function
FIIND domain / Function to find / UPA-FIIND domain / FIIND domain profile. / CARD8/ASC/NALP1, CARD domain / : / NACHT, LRR and PYD domains-containing protein, helical domain HD2 / NLRC4 helical domain HD2 / NOD2, winged helix domain / NOD2 winged helix domain ...FIIND domain / Function to find / UPA-FIIND domain / FIIND domain profile. / CARD8/ASC/NALP1, CARD domain / : / NACHT, LRR and PYD domains-containing protein, helical domain HD2 / NLRC4 helical domain HD2 / NOD2, winged helix domain / NOD2 winged helix domain / NACHT nucleoside triphosphatase / NACHT domain / NACHT-NTPase domain profile. / DAPIN domain / DAPIN domain profile. / PAAD/DAPIN/Pyrin domain / PAAD/DAPIN/Pyrin domain / Leucine rich repeat, ribonuclease inhibitor type / Leucine Rich repeat / CARD domain / CARD caspase recruitment domain profile. / Caspase recruitment domain / Leucine Rich Repeat / Death-like domain superfamily / Leucine-rich repeat profile. / Leucine-rich repeat / Leucine-rich repeat domain superfamily / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
NACHT, LRR and PYD domains-containing protein 1
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 3.7 Å
AuthorsGong, Q. / Xu, C. / Zhang, J. / Wu, B.
Funding support Singapore, 1items
OrganizationGrant numberCountry
Ministry of Education (Singapore)tier2 and tier1 Singapore
CitationJournal: Nat Commun / Year: 2021
Title: Structural basis for distinct inflammasome complex assembly by human NLRP1 and CARD8.
Authors: Qin Gong / Kim Robinson / Chenrui Xu / Phuong Thao Huynh / Kelvin Han Chung Chong / Eddie Yong Jun Tan / Jiawen Zhang / Zhao Zhi Boo / Daniel Eng Thiam Teo / Kenneth Lay / Yaming Zhang / ...Authors: Qin Gong / Kim Robinson / Chenrui Xu / Phuong Thao Huynh / Kelvin Han Chung Chong / Eddie Yong Jun Tan / Jiawen Zhang / Zhao Zhi Boo / Daniel Eng Thiam Teo / Kenneth Lay / Yaming Zhang / John Soon Yew Lim / Wah Ing Goh / Graham Wright / Franklin L Zhong / Bruno Reversade / Bin Wu /
Abstract: Nod-like receptor (NLR) proteins activate pyroptotic cell death and IL-1 driven inflammation by assembling and activating the inflammasome complex. Closely related sensor proteins NLRP1 and CARD8 ...Nod-like receptor (NLR) proteins activate pyroptotic cell death and IL-1 driven inflammation by assembling and activating the inflammasome complex. Closely related sensor proteins NLRP1 and CARD8 undergo unique auto-proteolysis-dependent activation and are implicated in auto-inflammatory diseases; however, their mechanisms of activation are not understood. Here we report the structural basis of how the activating domains (FIIND-CARD) of NLRP1 and CARD8 self-oligomerize to assemble distinct inflammasome complexes. Recombinant FIIND-CARD of NLRP1 forms a two-layered filament, with an inner core of oligomerized CARD surrounded by an outer ring of FIIND. Biochemically, self-assembled NLRP1-CARD filaments are sufficient to drive ASC speck formation in cultured human cells-a process that is greatly enhanced by NLRP1-FIIND which forms oligomers in vitro. The cryo-EM structures of NLRP1-CARD and CARD8-CARD filaments, solved here at 3.7 Å, uncover unique structural features that enable NLRP1 and CARD8 to discriminate between ASC and pro-caspase-1. In summary, our findings provide structural insight into the mechanisms of activation for human NLRP1 and CARD8 and reveal how highly specific signaling can be achieved by heterotypic CARD interactions within the inflammasome complexes.
History
DepositionJun 9, 2019Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Sep 16, 2020Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2021Group: Database references / Structure summary / Category: audit_author / citation / citation_author
Item: _audit_author.name / _citation.country ..._audit_author.name / _citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Mar 27, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

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Assembly

Deposited unit
J: NACHT, LRR and PYD domains-containing protein 1
A: NACHT, LRR and PYD domains-containing protein 1
B: NACHT, LRR and PYD domains-containing protein 1
C: NACHT, LRR and PYD domains-containing protein 1
D: NACHT, LRR and PYD domains-containing protein 1
E: NACHT, LRR and PYD domains-containing protein 1
F: NACHT, LRR and PYD domains-containing protein 1
G: NACHT, LRR and PYD domains-containing protein 1
H: NACHT, LRR and PYD domains-containing protein 1
I: NACHT, LRR and PYD domains-containing protein 1
K: NACHT, LRR and PYD domains-containing protein 1
L: NACHT, LRR and PYD domains-containing protein 1


Theoretical massNumber of molelcules
Total (without water)146,44512
Polymers146,44512
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: assay for oligomerization
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Protein
NACHT, LRR and PYD domains-containing protein 1 / Caspase recruitment domain-containing protein 7 / Death effector filament-forming ced-4-like ...Caspase recruitment domain-containing protein 7 / Death effector filament-forming ced-4-like apoptosis protein / Nucleotide-binding domain and caspase recruitment domain


Mass: 12203.776 Da / Num. of mol.: 12
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: NLRP1, CARD7, DEFCAP, KIAA0926, NAC, NALP1 / Production host: Escherichia coli K-12 (bacteria) / Strain (production host): K-12 / References: UniProt: Q9C000

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: FILAMENT / 3D reconstruction method: helical reconstruction

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Sample preparation

ComponentName: NLRP1 CARD filament / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT
Source (natural)Organism: Homo sapiens (human)
Source (recombinant)Organism: Escherichia coli (E. coli) / Strain: Bl21(DE3)
Buffer solutionpH: 8
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid material: COPPER / Grid mesh size: 300 divisions/in. / Grid type: Quantifoil R1.2/1.3
VitrificationInstrument: FEI VITROBOT MARK III / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277.15 K

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: DARK FIELD / Nominal magnification: 115000 X / Nominal defocus max: 2500 nm / Nominal defocus min: 1500 nm / Cs: 2.7 mm / Alignment procedure: COMA FREE
Specimen holderCryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingElectron dose: 80 e/Å2 / Detector mode: COUNTING / Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Num. of real images: 2187
Image scansMovie frames/image: 40 / Used frames/image: 5-35

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Processing

SoftwareName: PHENIX / Version: (dev_2405: ???) / Classification: refinement
EM software
IDNameVersionCategory
2EPU2.3image acquisition
4RELION2CTF correction
7Coot0.8.9.1 ELmodel fitting
9RELION2initial Euler assignment
10RELION2final Euler assignment
11RELION2classification
12RELION23D reconstruction
13PHENIXdev 2405model refinement
CTF correctionType: NONE
Helical symmertyAngular rotation/subunit: -100.821 ° / Axial rise/subunit: 5.36 Å / Axial symmetry: C1
Particle selectionNum. of particles selected: 422388
3D reconstructionResolution: 3.7 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 53241 / Symmetry type: HELICAL
Atomic model buildingProtocol: RIGID BODY FIT / Space: REAL
RefinementResolution: 3.7→85.8 Å / SU ML: 1.62 / σ(F): 0.04 / Phase error: 66.01 / Stereochemistry target values: MLHL
RfactorNum. reflection% reflection
Rfree0.2846 1285 4.37 %
Rwork0.2548 --
obs0.2561 29384 99.9 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.0058652
ELECTRON MICROSCOPYf_angle_d0.76711652
ELECTRON MICROSCOPYf_dihedral_angle_d23.1885292
ELECTRON MICROSCOPYf_chiral_restr0.0411272
ELECTRON MICROSCOPYf_plane_restr0.0041476
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.7002-3.84830.55841340.55373102ELECTRON MICROSCOPY100
3.8483-4.02350.53111520.55653145ELECTRON MICROSCOPY100
4.0235-4.23560.54051300.53743114ELECTRON MICROSCOPY100
4.2356-4.50090.54941470.52823147ELECTRON MICROSCOPY100
4.5009-4.84840.46831440.48963115ELECTRON MICROSCOPY100
4.8484-5.33630.49651410.4273166ELECTRON MICROSCOPY100
5.3363-6.10830.45371380.40243113ELECTRON MICROSCOPY100
6.1083-7.6950.39531340.36163121ELECTRON MICROSCOPY100
7.695-85.8230.18091650.1233076ELECTRON MICROSCOPY99
Refinement TLS params.

Method: refined / Refine-ID: ELECTRON MICROSCOPY

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.82620.6058-1.5352.7057-1.75344.5929-0.50420.75420.1313-1.90070.51760.4779-2.00572.1926-0.22195.31281.63140.48455.63290.43171.0138111.8992147.4816142.949
22.45020.6009-2.82286.4845-0.28963.267-1.49461.24372.5173-0.2947-0.3078-1.222-1.13150.52431.25841.3979-0.3187-0.7174.61612.04213.5171127.4096153.0505141.7776
32.85744.5351-2.36588.487-7.15852.00991.27273.6885-2.4077-3.1119-4.7889-1.6725.08124.7283.75952.63610.9511-0.40685.5061.30817.5411127.3453139.8172143.7372
46.559-4.2597-2.43172.01721.24399.671.34480.5698-0.5495-1.1819-1.00110.72222.2023-1.16370.54626.14071.79870.80345.23651.59221.7579118.1387142.8501147.3578
53.8068-0.0391-2.03630.6996-1.42734.14970.114-1.14080.20050.8128-0.46460.1623-2.69970.7059-0.17738.27111.0048-1.74081.88640.60530.4022116.9347156.5623143.4195
60.9889-0.55810.05221.56641.86642.8278-1.6477-0.56871.88171.39551.20662.0671-2.5285-1.39840.34880.7112.5492-0.48710.8311.33353.0455104.2903152.4878136.953
72.16832.04991.91191.94081.87642.59273.65010.5704-2.57466.7145-1.9144-0.76232.45910.3003-2.45762.7306-1.0824-0.34822.6756-0.41511.5115104.5391104.4282143.6937
84.01395.85812.03818.92033.04461.924.40481.0847-0.1101-3.9033-3.25491.5179-0.95741.56210.65764.34651.2847-0.6173.5523-0.56951.8893108.6919115.9453135.1044
90.27320.80630.27195.47176.91122.0059-3.97350.44971.213-2.90773.2509-8.5885-1.30537.95891.96273.90550.89250.31833.22681.31225.3753114.1996126.2311138.4644
107.36999.2232-1.92511.994-3.41415.0264-0.1025-1.0844-3.48970.8407-0.3754-4.8661.87723.05560.43895.08960.4834-0.66934.9978-0.49722.2341112.9189116.5334142.0279
114.9195-1.3319-0.23784.3451-0.45820.0762-0.34331.1965-0.12570.8229-2.38180.3432-3.1889-5.82950.25592.9333-0.9395-0.44716.968-0.35910.894299.7039112.8012137.89
122.55471.75870.10781.28670.20030.10590.83980.1306-1.74790.2426-0.53571.02050.75-0.2653-2.25940.374-0.2659-1.8189-0.6765-0.26131.2849106.2536101.205131.4614
133.519-1.46441.27911.8080.4041.22021.4403-0.43110.61223.1970.43581.8675-2.1149-1.0303-1.8323.7432-2.0581-0.26972.75592.9992.4382111.2132100.0501181.1982
144.2938-3.9463-0.59764.27030.43050.11573.40464.1353-1.0096-3.2651-5.26030.95881.02962.5341.7063.10111.25210.06983.4622-0.01982.3028112.4309112.0131172.7433
151.98210.10372.57853.34724.1438.1422-1.3541-0.8623-0.2804-1.3241-0.9532-4.28020.95478.15261.05911.9899-0.81362.15424.9083-0.53545.4682115.3383123.3123175.8517
161.75192.76512.95694.33094.69855.53443.52792.6293.50963.73550.2499-8.26131.17185.8982-2.9353.90420.04650.57754.3869-0.23284.685116.3209113.595179.556
170.3957-0.14460.42512.53731.45621.51542.3525-0.14620.82483.76770.25912.3682-0.3948-2.8258-0.72423.1949-3.72740.97373.95121.70760.8236104.409106.9523175.4512
184.8811.5483-4.04663.9317-1.8055.6137-0.98020.6942-4.1361-1.3006-2.4239-1.62993.26520.67352.0431.14770.17950.93581.88970.67751.9274113.463297.0483169.0377
190.2125-0.6999-0.19772.16350.48260.11271.60960.8613-1.03742.11312.4329-0.86052.0492-0.6424-1.28964.6771-0.1198-0.63673.25010.89191.7684135.4647102.4634155.8286
205.74743.3531-3.91177.6635-0.1166.35743.04465.77796.33-0.51821.02312.6517-1.80281.4985-4.35882.31471.3060.84744.8704-0.32584.7581120.1512111.3389152.2206
217.6033-4.90.15.22052.52033.5888-1.9136-2.19590.84641.81623.3554-2.30912.29481.6378-0.45424.58790.0955-0.36183.91290.18152.4853126.2845103.2779155.8672
224.6264-1.21571.72355.2491-0.09760.6432-0.1443-0.3776-2.17811.1816-0.3169-1.31721.9518-0.01971.01312.3374-1.07170.8752-1.2861.51592.7339137.783796.3973147.4209
237.3328-4.6284.98313.4068-2.45144.3527-0.329-1.2938-0.60310.4030.99540.5430.6161-0.0802-0.74692.92062.0138-1.92183.41091.19551.1351153.1857110.5613138.2674
242.08742.795-2.08473.7962-2.50033.46160.74492.50880.4357-2.52462.5775-0.01850.8098-2.6433-0.94924.8021.2265-0.80344.145-0.3661.6214147.7791119.2543131.4428
255.83754.1621-3.99332.0008-0.77313.2416-0.88921.79210.0483-7.86750.5033.51570.0775-2.77261.27324.389-0.7325-0.79496.8455-2.82422.8065137.3081110.506124.9587
263.18292.1983-2.76854.5309-2.10423.1919-2.3361-1.5455-0.51573.15142.03036.15583.3094-3.3299-0.84363.4371-1.5841.3133.9255-0.3224.0785134.2397103.2432132.2421
276.88757.38951.55948.01072.41815.8457-1.78850.31392.64262.47381.42679.7258-2.3504-9.01831.6423.70080.20970.83333.90531.26996.1258129.8687115.6158133.2562
287.6932-3.54132.75052.4005-1.68971.2866-1.4249-2.10522.472-1.1692.8702-1.03970.92331.05450.16395.59922.17170.00233.39720.73912.1851142.9905109.7163134.4599
298.31430.1264-4.19471.98251.24172.2734-1.4498-0.5739-0.8219-0.11441.2957-2.8750.37890.3219-0.4210.06830.25-0.51730.4720.31563.0559155.9834112.7936126.1042
303.7742-2.46091.46961.9761-0.35481.53260.51721.52-1.0846-1.0516-0.02060.44841.83631.2602-0.45044.24991.7542-2.86173.7785-0.81571.6726156.4473117.6655176.0408
315.9914-2.04271.79323.5198-5.29558.3028-2.10380.07131.7621-1.60170.3571-1.19410.1499-2.7812.14084.5822-0.6263-1.26653.54780.54572.2053149.4114124.8458169.3979
326.4496-3.8302-0.93423.19452.04032.5443-1.8955-1.2206-2.88810.98872.59170.89540.19090.92810.733.1978-1.65233.93234.2048-1.41442.5228140.8504110.174165.9723
333.14523.10022.0073.15782.04842.21851.04771.36051.5788-0.71220.04325.0759-4.60681.6516-0.33454.57211.16680.43713.58180.59995.7553132.9591117.0809170.3363
342.2588-3.2086-1.98834.68573.99581.99570.7873-4.48854.5603-3.24941.35942.2108-5.416-0.6027-1.24543.1542-0.1181-0.42434.74430.47392.4257142.1413120.0442174.3422
352.4202-3.47610.38425.6407-2.33053.0797-1.3094-3.40891.9420.70710.5684-1.96050.45912.31292.6166-0.21273.9104-2.1832.65731.43681.5944154.3588114.2266167.6121
365.48370.1785-4.1033.1836-0.49186.5359-0.2155-0.6033-0.1530.2-0.20820.40840.142-0.3262-0.26581.9731.941.4745.53632.2162.706594.8777125.7852165.1644
375.16860.0443-7.65980.3836-0.58241.9563-2.7859-1.2939-0.3181-0.75192.5849-0.35573.01071.9662-1.8085.0295-0.569-0.9043.0688-0.57611.766105.6164131.3044156.5106
385.66355.2776-5.39494.9468-4.93985.9085-3.38791.0516-0.933-0.09591.1727-4.6441.89822.33333.32083.30060.7527-0.54884.308-2.14795.0437116.7583134.8553159.5514
397.0730.49861.93894.92381.96241.2012-0.32541.4098-5.1481-0.7652-0.8941-2.77493.1792-0.87381.03324.8302-0.89870.24673.2219-0.00382.6995109.2312128.8812163.2803
400.51450.67120.09281.80130.43291.7613-0.7368-0.0904-0.15450.7126-1.0732.275-1.2976-2.54650.55341.26993.29330.22574.1277-0.94681.673797.1385135.1952159.4804
414.01591.5669-1.1263.62620.73320.77810.6791-0.4908-3.6049-0.91250.30215.1097-0.4454-2.1066-0.99690.71762.0087-1.14053.91-0.34252.983293.5883122.3201152.8727
422.9755-1.7138-1.69792.74573.41827.2140.3502-0.52540.82120.11170.9111-0.8349-1.17420.7554-0.70085.71510.30860.4611.3361-0.87121.0995130.4301158.8933170.7183
434.9751-0.67571.80881.5014-2.15534.4655-0.02252.83931.3917-0.5136-1.122-0.51412.63040.74780.54643.51210.3021-0.8925.07430.30281.9603133.9194147.4714162.1143
445.19252.16415.46347.48553.50625.94481.29080.5446-7.7881-3.378-2.5878-4.5841-0.1998-3.88731.04123.08660.17631.11623.99110.33557.2634135.6304135.9091164.9814
452.9817-1.96621.72554.5261.72453.69733.17772.4075-4.46764.43531.0013.6653-3.8049-3.1905-2.87373.74080.10590.13533.7997-0.14882.8683130.8917144.4101168.9425
465.05043.19930.89883.7228-0.13454.10310.7903-0.61182.67133.90920.16711.0454-2.77550.78441.82124.8039-1.4645-0.9462-1.2882-0.56852.3087134.0902157.5477162.3749
472.25723.36611.40854.88472.0370.89942.4816-0.63951.0428-1.5036-1.81210.39372.49250.8164-0.13823.94780.6677-0.34543.8316-0.11371.4929149.6553141.2104150.7253
483.9438-2.8511.30835.6137-4.09773.23891.41423.0265-2.5554-4.999-3.12730.0934-0.0418-0.36062.28744.81951.33250.99211.76540.55741.5034148.8891124.7179144.5291
494.4814.25931.99454.46631.9462.0343-3.53891.2791-0.2161-0.30431.69587.05741.26331.99323.77094.1992-0.81160.51516.1231.15865.3305138.471124.1997149.1209
502.69352.60590.7776.53252.41530.91050.0909-3.87693.30263.8239-2.16661.4130.56081.22051.70863.14570.1128-0.10715.15610.30871.8989150.5165132.1526150.6205
516.5872-4.35951.74552.9109-1.1348.39510.24610.90174.7354-1.2373-2.8265-5.176-0.03554.65362.24842.49410.0726-0.31882.16190.56963.4278155.0751144.7226142.3371
526.5007-2.64621.93343.1442-1.87431.1482-0.2618-0.94810.54941.2790.2167-1.4509-0.22760.1430.05613.6631-1.2425-0.83823.0573-1.54552.0234137.9662157.4086132.9083
532.00771.93081.42537.2753-1.44768.5438-0.95823.1699-0.2025-1.7571-0.97710.10885.74480.36091.17414.6183-0.33981.1217.21080.16241.2606135.0858146.9973123.3457
545.0352-0.16722.62067.06851.48121.7128-0.00741.48480.47991.9947-1.3236-0.69750.27420.89680.31084.3932.36750.68072.30741.34125.3642149.016140.3824126.9081
554.64610.1343-3.82641.11911.59752.00720.26162.7094-1.3672.2263-1.96841.54630.6421-4.19072.41554.53882.36420.53233.8834-1.25576.2266137.8465133.5625127.8453
564.9083-5.86891.79417.9229-0.95232.59643.59574.02390.49394.3874-2.23196.2092-1.7599-2.48860.0524.61770.18560.78524.1928-0.10773.1514135.1782142.93131.2484
573.44581.01141.21323.33620.80731.1738-0.2620.13090.02510.3839-0.65390.4022-3.31363.889-0.07424.597-2.95260.04394.02010.3692.1791145.9152151.3584127.3132
581.2979-0.2172-1.26143.1545-1.18992.80540.20970.522.02680.7031-0.6661-0.9902-2.19120.17780.36232.57560.7876-2.1413-0.11032.53182.3422135.3916159.6299120.8449
591.80770.60123.19297.10620.04835.7921-0.2645-3.75911.07851.4293-0.41910.62930.95110.7186-0.01770.9033-0.18351.1682.9733-0.09181.836395.3859133.315127.4893
605.2852-3.21666.51721.9948-4.13961.99740.5635-1.6959-1.809-3.07161.29430.08433.60883.5925-0.33655.22871.7131-0.12833.5611-0.35661.5461103.5815127.3643120.5965
618.8218-9.0544.82789.3047-4.95832.636-0.53543.65412.9515-2.36520.167-0.46080.38562.28711.19774.682-0.1035-2.39094.29391.82672.4916110.0391139.4411114.2081
622.7237-1.78894.51612.4695-2.22837.9004-0.95352.6662.26620.20730.330.0945-0.3390.6634-0.3760.92730.5656-2.61153.4543-0.18034.4861110.1935147.1275121.544
636.2585.46963.97024.78613.47252.50522.7222-1.5667-7.06191.7404-0.6009-5.47720.8784.3052-1.7033.7528-0.2977-0.37933.55130.56654.3758118.872137.2992122.263
643.414-1.6830.92771.6875-0.03951.0590.27490.0517-2.6258-0.36140.6090.34371.04670.61450.91593.03561.9223-0.66293.1997-1.26773.1904110.0622133.0287125.8152
655.0793-1.39711.91964.2581.32953.1974-0.6624-1.81650.39651.01540.5349-0.3311-1.9847-2.0471-0.57933.04243.1017-1.33166.2976-0.49611.033699.7571142.1383121.9447
665.3424-2.56951.03531.2752-0.35491.5429-0.0201-1.405-0.3761-0.2329-0.68242.5090.6209-1.3641-1.32291.5243-1.6239-0.44531.8433-0.4491.954993.5124130.327115.4199
672.1182-2.64591.20317.066-3.65163.7569-1.787-0.6882-0.0012.53661.00360.5125-0.1071-0.05730.39474.896-0.0012-0.28711.96411.31421.3344126.591795.8755122.1707
684.0047-4.7618-3.70197.74394.38173.3754-2.32414.92010.4896-0.5927-1.06111.6857-3.6449-0.28171.884.18120.643-0.89364.273-0.1532.422121.6544107.1174113.6764
692.10411.752.13414.93776.56878.7297-0.25012.09794.0424-0.53850.488-0.277-4.7395-1.8534-1.12955.1870.5776-1.05264.2775-0.03154.5413118.5751118.248117.1959
707.50233.2941-0.88794.3051.67391.7051-1.42333.7996-0.15551.59892.8627-1.65121.2531-2.59940.82486.78650.08-1.12962.4558-0.86281.3069120.3569104.0708118.428
711.4157-4.0581-3.16531.98631.99592.0114-3.25673.2779-3.69492.86990.9017-2.88065.0367-1.37941.04652.2968-0.18120.50473.3842-0.73213.0439129.940794.6676110.022
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1ELECTRON MICROSCOPY1chain 'J' and (resid 1379 through 1401 )
2ELECTRON MICROSCOPY2chain 'J' and (resid 1402 through 1409 )
3ELECTRON MICROSCOPY3chain 'J' and (resid 1410 through 1421 )
4ELECTRON MICROSCOPY4chain 'J' and (resid 1422 through 1435 )
5ELECTRON MICROSCOPY5chain 'J' and (resid 1436 through 1451 )
6ELECTRON MICROSCOPY6chain 'J' and (resid 1452 through 1462 )
7ELECTRON MICROSCOPY7chain 'A' and (resid 1379 through 1385 )
8ELECTRON MICROSCOPY8chain 'A' and (resid 1386 through 1409 )
9ELECTRON MICROSCOPY9chain 'A' and (resid 1410 through 1421 )
10ELECTRON MICROSCOPY10chain 'A' and (resid 1422 through 1435 )
11ELECTRON MICROSCOPY11chain 'A' and (resid 1436 through 1451 )
12ELECTRON MICROSCOPY12chain 'A' and (resid 1452 through 1462 )
13ELECTRON MICROSCOPY13chain 'B' and (resid 1379 through 1385 )
14ELECTRON MICROSCOPY14chain 'B' and (resid 1386 through 1409 )
15ELECTRON MICROSCOPY15chain 'B' and (resid 1410 through 1421 )
16ELECTRON MICROSCOPY16chain 'B' and (resid 1422 through 1435 )
17ELECTRON MICROSCOPY17chain 'B' and (resid 1436 through 1451 )
18ELECTRON MICROSCOPY18chain 'B' and (resid 1452 through 1462 )
19ELECTRON MICROSCOPY19chain 'C' and (resid 1379 through 1395 )
20ELECTRON MICROSCOPY20chain 'C' and (resid 1396 through 1421 )
21ELECTRON MICROSCOPY21chain 'C' and (resid 1422 through 1451 )
22ELECTRON MICROSCOPY22chain 'C' and (resid 1452 through 1462 )
23ELECTRON MICROSCOPY23chain 'D' and (resid 1379 through 1385 )
24ELECTRON MICROSCOPY24chain 'D' and (resid 1386 through 1395 )
25ELECTRON MICROSCOPY25chain 'D' and (resid 1396 through 1402 )
26ELECTRON MICROSCOPY26chain 'D' and (resid 1403 through 1409 )
27ELECTRON MICROSCOPY27chain 'D' and (resid 1410 through 1421 )
28ELECTRON MICROSCOPY28chain 'D' and (resid 1422 through 1451 )
29ELECTRON MICROSCOPY29chain 'D' and (resid 1452 through 1462 )
30ELECTRON MICROSCOPY30chain 'E' and (resid 1379 through 1385 )
31ELECTRON MICROSCOPY31chain 'E' and (resid 1386 through 1395 )
32ELECTRON MICROSCOPY32chain 'E' and (resid 1396 through 1409 )
33ELECTRON MICROSCOPY33chain 'E' and (resid 1410 through 1421 )
34ELECTRON MICROSCOPY34chain 'E' and (resid 1422 through 1435 )
35ELECTRON MICROSCOPY35chain 'E' and (resid 1436 through 1462 )
36ELECTRON MICROSCOPY36chain 'F' and (resid 1379 through 1385 )
37ELECTRON MICROSCOPY37chain 'F' and (resid 1386 through 1409 )
38ELECTRON MICROSCOPY38chain 'F' and (resid 1410 through 1421 )
39ELECTRON MICROSCOPY39chain 'F' and (resid 1422 through 1435 )
40ELECTRON MICROSCOPY40chain 'F' and (resid 1436 through 1451 )
41ELECTRON MICROSCOPY41chain 'F' and (resid 1452 through 1462 )
42ELECTRON MICROSCOPY42chain 'G' and (resid 1379 through 1385 )
43ELECTRON MICROSCOPY43chain 'G' and (resid 1386 through 1409 )
44ELECTRON MICROSCOPY44chain 'G' and (resid 1410 through 1421 )
45ELECTRON MICROSCOPY45chain 'G' and (resid 1422 through 1435 )
46ELECTRON MICROSCOPY46chain 'G' and (resid 1436 through 1462 )
47ELECTRON MICROSCOPY47chain 'H' and (resid 1379 through 1395 )
48ELECTRON MICROSCOPY48chain 'H' and (resid 1396 through 1409 )
49ELECTRON MICROSCOPY49chain 'H' and (resid 1410 through 1421 )
50ELECTRON MICROSCOPY50chain 'H' and (resid 1422 through 1451 )
51ELECTRON MICROSCOPY51chain 'H' and (resid 1452 through 1462 )
52ELECTRON MICROSCOPY52chain 'I' and (resid 1379 through 1385 )
53ELECTRON MICROSCOPY53chain 'I' and (resid 1386 through 1402 )
54ELECTRON MICROSCOPY54chain 'I' and (resid 1403 through 1409 )
55ELECTRON MICROSCOPY55chain 'I' and (resid 1410 through 1421 )
56ELECTRON MICROSCOPY56chain 'I' and (resid 1422 through 1435 )
57ELECTRON MICROSCOPY57chain 'I' and (resid 1436 through 1451 )
58ELECTRON MICROSCOPY58chain 'I' and (resid 1452 through 1462 )
59ELECTRON MICROSCOPY59chain 'K' and (resid 1379 through 1385 )
60ELECTRON MICROSCOPY60chain 'K' and (resid 1386 through 1395 )
61ELECTRON MICROSCOPY61chain 'K' and (resid 1396 through 1402 )
62ELECTRON MICROSCOPY62chain 'K' and (resid 1403 through 1409 )
63ELECTRON MICROSCOPY63chain 'K' and (resid 1410 through 1421 )
64ELECTRON MICROSCOPY64chain 'K' and (resid 1422 through 1435 )
65ELECTRON MICROSCOPY65chain 'K' and (resid 1436 through 1451 )
66ELECTRON MICROSCOPY66chain 'K' and (resid 1452 through 1462 )
67ELECTRON MICROSCOPY67chain 'L' and (resid 1379 through 1385 )
68ELECTRON MICROSCOPY68chain 'L' and (resid 1386 through 1409 )
69ELECTRON MICROSCOPY69chain 'L' and (resid 1410 through 1421 )
70ELECTRON MICROSCOPY70chain 'L' and (resid 1422 through 1451 )
71ELECTRON MICROSCOPY71chain 'L' and (resid 1452 through 1462 )

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