+Open data
-Basic information
Entry | Database: PDB / ID: 6oqn | ||||||
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Title | Crystal structure of Mcl1 with inhibitor 7 | ||||||
Components | Induced myeloid leukemia cell differentiation protein Mcl-1 | ||||||
Keywords | APOPTOSIS / Protein-Protein Interaction / inhibitor | ||||||
Function / homology | Function and homology information positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway / cell fate determination / cellular homeostasis / Bcl-2 family protein complex / mitochondrial fusion / BH domain binding / BH3 domain binding / protein transmembrane transporter activity / negative regulation of anoikis / negative regulation of extrinsic apoptotic signaling pathway in absence of ligand ...positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway / cell fate determination / cellular homeostasis / Bcl-2 family protein complex / mitochondrial fusion / BH domain binding / BH3 domain binding / protein transmembrane transporter activity / negative regulation of anoikis / negative regulation of extrinsic apoptotic signaling pathway in absence of ligand / extrinsic apoptotic signaling pathway in absence of ligand / negative regulation of autophagy / release of cytochrome c from mitochondria / response to cytokine / channel activity / intrinsic apoptotic signaling pathway in response to DNA damage / Signaling by ALK fusions and activated point mutants / Interleukin-4 and Interleukin-13 signaling / regulation of apoptotic process / mitochondrial outer membrane / positive regulation of apoptotic process / protein heterodimerization activity / DNA damage response / negative regulation of apoptotic process / mitochondrion / nucleoplasm / membrane / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.7 Å | ||||||
Authors | Huang, X. | ||||||
Citation | Journal: Cancer Discov / Year: 2018 Title: AMG 176, a Selective MCL1 Inhibitor, Is Effective in Hematologic Cancer Models Alone and in Combination with Established Therapies. Authors: Caenepeel, S. / Brown, S.P. / Belmontes, B. / Moody, G. / Keegan, K.S. / Chui, D. / Whittington, D.A. / Huang, X. / Poppe, L. / Cheng, A.C. / Cardozo, M. / Houze, J. / Li, Y. / Lucas, B. / ...Authors: Caenepeel, S. / Brown, S.P. / Belmontes, B. / Moody, G. / Keegan, K.S. / Chui, D. / Whittington, D.A. / Huang, X. / Poppe, L. / Cheng, A.C. / Cardozo, M. / Houze, J. / Li, Y. / Lucas, B. / Paras, N.A. / Wang, X. / Taygerly, J.P. / Vimolratana, M. / Zancanella, M. / Zhu, L. / Cajulis, E. / Osgood, T. / Sun, J. / Damon, L. / Egan, R.K. / Greninger, P. / McClanaghan, J.D. / Gong, J. / Moujalled, D. / Pomilio, G. / Beltran, P. / Benes, C.H. / Roberts, A.W. / Huang, D.C. / Wei, A. / Canon, J. / Coxon, A. / Hughes, P.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6oqn.cif.gz | 84.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6oqn.ent.gz | 62.3 KB | Display | PDB format |
PDBx/mmJSON format | 6oqn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6oqn_validation.pdf.gz | 1 MB | Display | wwPDB validaton report |
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Full document | 6oqn_full_validation.pdf.gz | 1 MB | Display | |
Data in XML | 6oqn_validation.xml.gz | 19.8 KB | Display | |
Data in CIF | 6oqn_validation.cif.gz | 29.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/oq/6oqn ftp://data.pdbj.org/pub/pdb/validation_reports/oq/6oqn | HTTPS FTP |
-Related structure data
Related structure data | 6o6fC 6o6gC 6oqbC 6oqcC 6oqdC 6ovcC C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 17922.344 Da / Num. of mol.: 2 / Fragment: residues 171-327 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: MCL1, BCL2L3 / Production host: Escherichia coli (E. coli) / References: UniProt: Q07820 #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.27 Å3/Da / Density % sol: 45.9 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / Details: 100 mM Tris, pH 8.0 3% Methanol 30%-42.5% PEG 6000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU FR-E SUPERBRIGHT / Wavelength: 1.54 Å |
Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: Apr 29, 2010 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→30 Å / Num. obs: 34986 / % possible obs: 95 % / Redundancy: 3.8 % / Net I/σ(I): 14 |
Reflection shell | Resolution: 1.7→1.76 Å / Num. unique obs: 2487 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.7→29.21 Å / Cross valid method: THROUGHOUT
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Refinement step | Cycle: LAST / Resolution: 1.7→29.21 Å
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