- PDB-6gwf: Alpha-galactosidase mutant D387A from Thermotoga maritima in comp... -
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基本情報
登録情報
データベース: PDB / ID: 6gwf
タイトル
Alpha-galactosidase mutant D387A from Thermotoga maritima in complex with intact cyclohexene-based carbasugar mimic of galactose with 2,4-dinitro leaving group
分子量: 66154.086 Da / 分子数: 1 / 由来タイプ: 組換発現 詳細: Electron density at the N- and C-terminus was disordered and could not be modelled in the structure. Residue D387 was mutated to alanine in order to trap a complex with substrate. 由来: (組換発現) Thermotoga maritima MSB8 (バクテリア) 株: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099 / 遺伝子: galA, TM_1192, Tmari_1199 発現宿主: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (大腸菌) 参照: UniProt: G4FEF4, alpha-galactosidase
解像度: 1.72→55.49 Å / Cor.coef. Fo:Fc: 0.971 / Cor.coef. Fo:Fc free: 0.954 / SU B: 2.922 / SU ML: 0.086 / 交差検証法: THROUGHOUT / ESU R: 0.097 / ESU R Free: 0.099 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.20256
3487
5.2 %
RANDOM
Rwork
0.16452
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obs
0.16643
63960
99.48 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK