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- EMDB-6878: Cryo-EM structure of a nucleolar pre-60S ribosome (Rpf1-TAP) -

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基本情報

登録情報
データベース: EMDB / ID: EMD-6878
タイトルCryo-EM structure of a nucleolar pre-60S ribosome (Rpf1-TAP)
マップデータUnmasked cryo-EM map
試料
  • 複合体: Rpf1-TAP pre-60S
    • RNA: x 3種
    • タンパク質・ペプチド: x 32種
キーワードribosome / pre-60S / pre-ribosome / protein-RNA complex
機能・相同性
機能・相同性情報


snoRNA release from pre-rRNA / nuclear division / exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / PeBoW complex / ATP-dependent activity, acting on RNA / rRNA primary transcript binding / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of 5.8S rRNA / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) ...snoRNA release from pre-rRNA / nuclear division / exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / PeBoW complex / ATP-dependent activity, acting on RNA / rRNA primary transcript binding / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of 5.8S rRNA / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / preribosome, small subunit precursor / proteasome binding / Major pathway of rRNA processing in the nucleolus and cytosol / ribosomal large subunit binding / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / preribosome, large subunit precursor / Formation of a pool of free 40S subunits / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / L13a-mediated translational silencing of Ceruloplasmin expression / translational elongation / ribosomal large subunit export from nucleus / 90S preribosome / ribonucleoprotein complex binding / ribosomal subunit export from nucleus / maturation of LSU-rRNA / translation initiation factor activity / nuclear periphery / proteasome complex / assembly of large subunit precursor of preribosome / cytosolic ribosome assembly / ribosomal large subunit biogenesis / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / macroautophagy / maturation of SSU-rRNA / protein catabolic process / small-subunit processome / maintenance of translational fidelity / rRNA processing / nuclear envelope / ribosomal small subunit biogenesis / 5S rRNA binding / ribosomal large subunit assembly / large ribosomal subunit rRNA binding / protein-macromolecule adaptor activity / cytosolic large ribosomal subunit / cytoplasmic translation / RNA helicase activity / negative regulation of translation / rRNA binding / structural constituent of ribosome / RNA helicase / ribosome / translation / mRNA binding / nucleolus / ATP hydrolysis activity / RNA binding / zinc ion binding / nucleoplasm / ATP binding / identical protein binding / nucleus / cytosol / cytoplasm
類似検索 - 分子機能
WDR74/Nsa1 / Ribosomal RNA processing protein 1-like / Nucleolar protein,Nop52 / Mak16 protein / Mak16 protein C-terminal region / Ribosome biogenesis protein 15, RNA recognition motif / Eukaryotic rRNA processing / Eukaryotic rRNA processing protein EBP2 / Ribosome biogenesis protein BRX1 / DDX18/Has1, DEAD-box helicase domain ...WDR74/Nsa1 / Ribosomal RNA processing protein 1-like / Nucleolar protein,Nop52 / Mak16 protein / Mak16 protein C-terminal region / Ribosome biogenesis protein 15, RNA recognition motif / Eukaryotic rRNA processing / Eukaryotic rRNA processing protein EBP2 / Ribosome biogenesis protein BRX1 / DDX18/Has1, DEAD-box helicase domain / Domain of unknown function DUF4217 / ATP-dependent rRNA helicase SPB4-like, C-terminal extension / DUF4217 / U3 snoRNP protein/Ribosome production factor 1 / Pescadillo / Pescadillo N-terminus / BRCT domain / Brix domain / Brix domain / Brix domain profile. / Brix / Translation initiation factor IF6 / eIF-6 family / translation initiation factor 6 / DEAD-box subfamily ATP-dependent helicases signature. / ATP-dependent RNA helicase DEAD-box, conserved site / RNA helicase, DEAD-box type, Q motif / DEAD-box RNA helicase Q motif profile. / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / breast cancer carboxy-terminal domain / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / BRCT domain profile. / BRCT domain / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L1-like / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein L1p/L10e family / BRCT domain superfamily / : / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L6e signature. / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein L36e signature. / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L35Ae, conserved site / Ribosomal protein L35Ae signature. / Ribosomal Protein L6, KOW domain / 60S ribosomal protein L35 / Ribosomal protein L7A/L8 / Ribosomal protein L6e / Ribosomal protein L13, eukaryotic/archaeal / 60S ribosomal protein L6E / Ribosomal protein L7, eukaryotic / Ribosomal protein L30, N-terminal / Ribosomal L30 N-terminal domain / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L4 C-terminal domain / Ribosomal protein L18e / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal protein L36e / Ribosomal protein L14e domain / Ribosomal protein L14 / Ribosomal protein L32e, conserved site / Ribosomal protein L32e signature. / Ribosomal protein L35A / Ribosomal protein L35Ae / Ribosomal protein L35A superfamily / Ribosomal protein L6, conserved site-2 / Ribosomal protein L6 signature 2. / Ribosomal protein L14 / Ribosomal protein L15e, conserved site / Ribosomal protein L15e signature. / Ribosomal protein L4/L1e, eukaryotic/archaeal, conserved site / Ribosomal protein L1e signature. / Ribosomal protein L37e, conserved site / Ribosomal protein L37e signature. / Ribosomal protein L22/L17, eukaryotic/archaeal / Ribosomal protein L37e / Ribosomal protein L37e / Ribosomal_L15e / Ribosomal protein L4, eukaryotic and archaeal type / Ribosomal protein L15e / Ribosomal protein L15e core domain superfamily / Ribosomal L15 / DEAD/DEAH box helicase domain / Ribosomal protein L3, domain 3, archaeal type superfamily / Ribosomal protein L3, archaeal/eukaryotic type / DEAD/DEAH box helicase / Ribosomal protein L32e / Ribosomal protein L37ae/L37e / Ribosomal protein L32e superfamily / Ribosomal protein L32
類似検索 - ドメイン・相同性
Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL15A / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein eL18A / Large ribosomal subunit protein uL29A ...Large ribosomal subunit protein uL30A / Large ribosomal subunit protein uL6A / Large ribosomal subunit protein uL22A / Large ribosomal subunit protein uL24A / Large ribosomal subunit protein eL33A / Large ribosomal subunit protein eL36A / Large ribosomal subunit protein eL15A / Large ribosomal subunit protein eL20A / Large ribosomal subunit protein eL18A / Large ribosomal subunit protein uL29A / Large ribosomal subunit protein uL4A / Protein MAK16 / Large ribosomal subunit protein uL3 / Large ribosomal subunit protein eL8A / Large ribosomal subunit protein uL13A / Ribosomal RNA-processing protein 1 / rRNA-processing protein EBP2 / Large ribosomal subunit protein eL14A / Large ribosomal subunit protein eL32 / Proteasome-interacting protein CIC1 / Ribosome production factor 1 / Ribosome biogenesis protein RLP7 / Large ribosomal subunit protein eL37A / Ribosome biogenesis protein NSA1 / Pescadillo homolog / Ribosome biogenesis protein 15 / Large ribosomal subunit protein eL6A / ATP-dependent RNA helicase HAS1 / Ribosome biogenesis protein BRX1 / Eukaryotic translation initiation factor 6 / Large ribosomal subunit protein eL13A
類似検索 - 構成要素
生物種Saccharomyces cerevisiae (パン酵母) / Saccharomyces cerevisiae S288c (パン酵母)
手法単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.65 Å
データ登録者Zhou D / Zhu X / Ye K
引用ジャーナル: Protein Cell / 年: 2019
タイトル: Cryo-EM structure of an early precursor of large ribosomal subunit reveals a half-assembled intermediate.
著者: Dejian Zhou / Xing Zhu / Sanduo Zheng / Dan Tan / Meng-Qiu Dong / Keqiong Ye /
要旨: Assembly of eukaryotic ribosome is a complicated and dynamic process that involves a series of intermediates. It is unknown how the highly intertwined structure of 60S large ribosomal subunits is ...Assembly of eukaryotic ribosome is a complicated and dynamic process that involves a series of intermediates. It is unknown how the highly intertwined structure of 60S large ribosomal subunits is established. Here, we report the structure of an early nucleolar pre-60S ribosome determined by cryo-electron microscopy at 3.7 Å resolution, revealing a half-assembled subunit. Domains I, II and VI of 25S/5.8S rRNA pack tightly into a native-like substructure, but domains III, IV and V are not assembled. The structure contains 12 assembly factors and 19 ribosomal proteins, many of which are required for early processing of large subunit rRNA. The Brx1-Ebp2 complex would interfere with the assembly of domains IV and V. Rpf1, Mak16, Nsa1 and Rrp1 form a cluster that consolidates the joining of domains I and II. Our structure reveals a key intermediate on the path to establishing the global architecture of 60S subunits.
履歴
登録2018年1月6日-
ヘッダ(付随情報) 公開2018年4月11日-
マップ公開2018年4月11日-
更新2024年11月6日-
現状2024年11月6日処理サイト: PDBj / 状態: 公開

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構造の表示

ムービー
  • 表面図（断面を密度値に従い着色）
  • 表面レベル: 0.09
  • UCSF Chimeraによる作画
  • ダウンロード
  • 表面図（半径に従い着色）
  • 表面レベル: 0.09
  • UCSF Chimeraによる作画
  • ダウンロード
  • あてはめたモデルとの重ね合わせ
  • 原子モデル: PDB-5z3g
  • 表面レベル: 0.09
  • UCSF Chimeraによる作画
  • ダウンロード
ムービービューア
構造ビューアEMマップ:
SurfViewMolmilJmol/JSmol
添付画像

emd_6878.png

ダウンロードとリンク

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マップ

ファイルダウンロード / ファイル: emd_6878.map.gz / 形式: CCP4 / 大きさ: 178 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈Unmasked cryo-EM map
投影像・断面図

画像のコントロール

大きさ
明度
コントラスト
その他
軸Z (Sec.)Y (Row.)X (Col.)
1.38 Å/pix.
x 360 pix.
= 496.8 Å		1.38 Å/pix.
x 360 pix.
= 496.8 Å		1.38 Å/pix.
x 360 pix.
= 496.8 Å

表面

投影像

断面 (1/3)

断面 (1/2)

断面 (2/3)

画像は Spider により作成

ボクセルのサイズX=Y=Z: 1.38 Å
密度

ヒストグラム

ヒストグラム (対数)
表面レベル登録者による: 0.09 / ムービー #1: 0.09
最小 - 最大-0.2651229 - 0.5640423
平均 (標準偏差)0.0001233261 (±0.018730178)
対称性空間群: 1
詳細

EMDB XML:

マップ形状
Axis orderXYZ
Origin000
サイズ360360360
Spacing360360360
セルA=B=C: 496.8 Å
α=β=γ: 90.0 °

CCP4マップ ヘッダ情報:

modeImage stored as Reals
Å/pix. X/Y/Z1.381.381.38
M x/y/z360360360
origin x/y/z0.0000.0000.000
length x/y/z496.800496.800496.800
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS360360360
D min/max/mean-0.2650.5640.000

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添付データ

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試料の構成要素

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全体 : Rpf1-TAP pre-60S

全体名称: Rpf1-TAP pre-60S
要素
  • 複合体: Rpf1-TAP pre-60S
    • RNA: 25S rRNA
    • RNA: 5.8S rRNA
    • RNA: ITS2 RNA
    • タンパク質・ペプチド: Ribosome biogenesis protein RLP7
    • タンパク質・ペプチド: Ribosome biogenesis protein 15
    • タンパク質・ペプチド: 60S ribosomal protein L3
    • タンパク質・ペプチド: 60S ribosomal protein L4-A
    • タンパク質・ペプチド: Proteasome-interacting protein CIC1
    • タンパク質・ペプチド: 60S ribosomal protein L6-A
    • タンパク質・ペプチド: 60S ribosomal protein L7-A
    • タンパク質・ペプチド: 60S ribosomal protein L8-A
    • タンパク質・ペプチド: 60S ribosomal protein L9-A
    • タンパク質・ペプチド: Nucleolar protein 7
    • タンパク質・ペプチド: Eukaryotic translation initiation factor 6
    • タンパク質・ペプチド: Ribosome production factor 1
    • タンパク質・ペプチド: 60S ribosomal protein L13-A
    • タンパク質・ペプチド: 60S ribosomal protein L14-A
    • タンパク質・ペプチド: 60S ribosomal protein L15-A
    • タンパク質・ペプチド: 60S ribosomal protein L16-A
    • タンパク質・ペプチド: 60S ribosomal protein L17-A
    • タンパク質・ペプチド: 60S ribosomal protein L18-A
    • タンパク質・ペプチド: Ribosome biogenesis protein NSA1
    • タンパク質・ペプチド: 60S ribosomal protein L20-A
    • タンパク質・ペプチド: Ribosomal RNA-processing protein 1
    • タンパク質・ペプチド: ATP-dependent RNA helicase HAS1
    • タンパク質・ペプチド: Protein MAK16
    • タンパク質・ペプチド: Ribosome biogenesis protein BRX1
    • タンパク質・ペプチド: rRNA-processing protein EBP2
    • タンパク質・ペプチド: 60S ribosomal protein L26-A
    • タンパク質・ペプチド: Unassigned
    • タンパク質・ペプチド: 60S ribosomal protein L32
    • タンパク質・ペプチド: 60S ribosomal protein L33-A
    • タンパク質・ペプチド: 60S ribosomal protein L35-A
    • タンパク質・ペプチド: 60S ribosomal protein L36-A
    • タンパク質・ペプチド: 60S ribosomal protein L37-A

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超分子 #1: Rpf1-TAP pre-60S

超分子名称: Rpf1-TAP pre-60S / タイプ: complex / ID: 1 / 親要素: 0 / 含まれる分子: all
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)

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分子 #1: 25S rRNA

分子名称: 25S rRNA / タイプ: rna / ID: 1 / コピー数: 1
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 1.0974938749999998 MDa
配列文字列: GUUUGACCUC AAAUCAGGUA GGAGUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACCGGGAUUG CCUUAGUAA CGGCGAGUGA AGCGGCAAAA GCUCAAAUUU GAAAUCUGGU ACCUUCGGUG CCCGAGUUGU AAUUUGGAGA G GGCAACUU ...文字列:
GUUUGACCUC AAAUCAGGUA GGAGUACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACCGGGAUUG CCUUAGUAA CGGCGAGUGA AGCGGCAAAA GCUCAAAUUU GAAAUCUGGU ACCUUCGGUG CCCGAGUUGU AAUUUGGAGA G GGCAACUU UGGGGCCGUU CCUUGUCUAU GUUCCUUGGA ACAGGACGUC AUAGAGGGUG AGAAUCCCGU GUGGCGAGGA GU GCGGUUC UUUGUAAAGU GCCUUCGAAG AGUCGAGUUG UUUGGGAAUG CAGCUCUAAG UGGGUGGUAA AUUCCAUCUA AAG CUAAAU AUUGGCGAGA GACCGAUAGC GAACAAGUAC AGUGAUGGAA AGAUGAAAAG AACUUUGAAA AGAGAGUGAA AAAG UACGU GAAAUUGUUG AAAGGGAAGG GCAUUUGAUC AGACAUGGUG UUUUGUGCCC UCUGCUCCUU GUGGGUAGGG GAAUC UCGC AUUUCACUGG GCCAGCAUCA GUUUUGGUGG CAGGAUAAAU CCAUAGGAAU GUAGCUUGCC UCGGUAAGUA UUAUAG CCU GUGGGAAUAC UGCCAGCUGG GACUGAGGAC UGCGACGUAA GUCAAGGAUG CUGGCAUAAU GGUUAUAUGC CGCCCGU CU UGAAACACGG ACCAAGGAGU CUAACGUCUA UGCGAGUGUU UGGGUGUAAA ACCCAUACGC GUAAUGAAAG UGAACGUA G GUUGGGGCCU CGCAAGAGGU GCACAAUCGA CCGAUCCUGA UGUCUUCGGA UGGAUUUGAG UAAGAGCAUA GCUGUUGGG ACCCGAAAGA UGGUGAACUA UGCCUGAAUA GGGUGAAGCC AGAGGAAACU CUGGUGGAGG CUCGUAGCGG UUCUGACGUG CAAAUCGAU CGUCGAAUUU GGGUAUAGGG GCGAAAGACU AAUCGAACCA UCUAGUAGCU GGUUCCUGCC GAAGUUUCCC U CAGGAUAG CAGAAGCUCG UAUCAGUUUU AUGAGGUAAA GCGAAUGAUU AGAGGUUCCG GGGUCGAAAU GACCUUGACC UA UUCUCAA ACUUUAAAUA UGUAAGAAGU CCUUGUUACU UAAUUGAACG UGGACAUUUG AAUGAAGAGC UUUUAGUGGG CCA UUUUUG GUAAGCAGAA CUGGCGAUGC GGGAUGAACC GAACGUAGAG UUAAGGUGCC GGAAUACACG CUCAUCAGAC ACCA CAAAA GGUGUUAGUU CAUCUAGACA GCCGGACGGU GGCCAUGGAA GUCGGAAUCC GCUAAGGAGU GUGUAACAAC UCACC GGCC GAAUGAACUA GCCCUGAAAA UGGAUGGCGC UCAAGCGUGU UACCUAUACU CUACCGUCAG GGUUGAUAUG AUGCCC UGA CGAGUAGGCA GGCGUGGAGG UCAGUGACGA AGCCUAGACC GUAAGGUCGG GUCGAACGGC CUCUAGUGCA GAUCUUG GU GGUAGUAGCA AAUAUUCAAA UGAGAACUUU GAAGACUGAA GUGGGGAAAG GUUCCACGUC AACAGCAGUU GGACGUGG G UUAGUCGAUC CUAAGAGAUG GGGAAGCUCC GUUUCAAAGG CCUGAUUUUA UGCAGGCCAC CAUCGAAAGG GAAUCCGGU UAAGAUUCCG GAACCUGGAU AUGGAUUCUU CACGGUAACG UAACUGAAUG UGGAGACGUC GGCGCGAGCC CUGGGAGGAG UUAUCUUUU CUUCUUAACA GCUUAUCACC CCGGAAUUGG UUUAUCCGGA GAUGGGGUCU UAUGGCUGGA AGAGGCCAGC A CCUUUGCU GGCUCCGGUG CGCUUGUGAC GGCCCGUGAA AAUCCACAGG AAGGAAUAGU UUUCAUGCCA GGUCGUACUG AU AACCGCA GCAGGUCUCC AAGGUGAACA GCCUCUAGUU GAUAGAAUAA UGUAGAUAAG GGAAGUCGGC AAAAUAGAUC CGU AACUUC GGGAUAAGGA UUGGCUCUAA GGGUCGGGUA GUGAGGGCCU UGGUCAGACG CAGCGGGCGU GCUUGUGGAC UGCU UGGUG GGGCUUGCUC UGCUAGGCGG ACUACUUGCG UGCCUUGUUG UAGACGGCCU UGGUAGGUCU CUUGUAGACC GUCGC UUGC UACAAUUAAC GAUCAACUUA GAACUGGUAC GGACAAGGGG AAUCUGACUG UCUAAUUAAA ACAUAGCAUU GCGAUG GUC AGAAAGUGAU GUUGACGCAA UGUGAUUUCU GCCCAGUGCU CUGAAUGUCA AAGUGAAGAA AUUCAACCAA GCGCGGG UA AACGGCGGGA GUAACUAUGA CUCUCUUAAG GUAGCCAAAU GCCUCGUCAU CUAAUUAGUG ACGCGCAUGA AUGGAUUA A CGAGAUUCCC ACUGUCCCUA UCUACUAUCU AGCGAAACCA CAGCCAAGGG AACGGGCUUG GCAGAAUCAG CGGGGAAAG AAGACCCUGU UGAGCUUGAC UCUAGUUUGA CAUUGUGAAG AGACAUAGAG GGUGUAGAAU AAGUGGGAGC UUCGGCGCCA GUGAAAUAC CACUACCUUU AUAGUUUCUU UACUUAUUCA AUGAAGCGGA GCUGGAAUUC AUUUUCCACG UUCUAGCAUU C AAGGUCCC AUUCGGGGCU GAUCCGGGUU GAAGACAUUG UCAGGUGGGG AGUUUGGCUG GGGCGGCACA UCUGUUAAAC GA UAACGCA GAUGUCCUAA GGGGGGCUCA UGGAGAACAG AAAUCUCCAG UAGAACAAAA GGGUAAAAGC CCCCUUGAUU UUG AUUUUC AGUGUGAAUA CAAACCAUGA AAGUGUGGCC UAUCGAUCCU UUAGUCCCUC GGAAUUUGAG GCUAGAGGUG CCAG AAAAG UUACCACAGG GAUAACUGGC UUGUGGCAGU CAAGCGUUCA UAGCGACAUU GCUUUUUGAU UCUUCGAUGU CGGCU CUUC CUAUCAUACC GAAGCAGAAU UCGGUAAGCG UUGGAUUGUU CACCCACUAA UAGGGAACGU GAGCUGGGUU UAGACC GUC GUGAGACAGG UUAGUUUUAC CCUACUGAUG AAUGUUACCG CAAUAGUAAU UGAACUUAGU ACGAGAGGAA CAGUUCA UU CGGAUAAUUG GUUUUUGCGG CUGUCUGAUC AGGCAUUGCC GCGAAGCUAC CAUCCGCUGG AUUAUGGCUG AACGCCUC U AAGUCAGAAU CCAUGCUAGA ACGCGGUGAU UUCUUUGCUC CACACAAUAU AGAUGGAUAC GAAUAAGGCG UCCUUGUGG CGUCGCUGAA CCAUAGCAGG CUAGCAACGG UGCACUUGGC GGAAAGGCCU UGGGUGCUUG CUGGCGAAUU GCAAUGUCAU UUUGCGUGG GGAUAAAUCA UUUGUAUACG ACUUAGAUGU ACAACGGGGU AUUGUAAGCA GUAGAGUAGC CUUGUUGUUA C GAUCUGCU GAGAUUAAGC CUUUGUUGUC UGAUUUGU

GENBANK: GENBANK: CP011821.1

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分子 #2: 5.8S rRNA

分子名称: 5.8S rRNA / タイプ: rna / ID: 2 / コピー数: 1
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 50.682922 KDa
配列文字列:
AAACUUUCAA CAACGGAUCU CUUGGUUCUC GCAUCGAUGA AGAACGCAGC GAAAUGCGAU ACGUAAUGUG AAUUGCAGAA UUCCGUGAA UCAUCGAAUC UUUGAACGCA CAUUGCGCCC CUUGGUAUUC CAGGGGGCAU GCCUGUUUGA GCGUCAUUU

GENBANK: GENBANK: MG214540.1

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分子 #3: ITS2 RNA

分子名称: ITS2 RNA / タイプ: rna / ID: 3 / コピー数: 1
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 74.308391 KDa
配列文字列: CCUUCUCAAA CAUUCUGUUU GGUAGUGAGU GAUACUCUUU GGAGUUAACU UGAAAUUGCU GGCCUUUUCA UUGGAUGUUU UUUUUCCAA AGAGAGGUUU CUCUGCGUGC UUGAGGUAUA AUGCAAGUAC GGUCGUUUUA GGUUUUACCA ACUGCGGCUA A UCUUUUUU ...文字列:
CCUUCUCAAA CAUUCUGUUU GGUAGUGAGU GAUACUCUUU GGAGUUAACU UGAAAUUGCU GGCCUUUUCA UUGGAUGUUU UUUUUCCAA AGAGAGGUUU CUCUGCGUGC UUGAGGUAUA AUGCAAGUAC GGUCGUUUUA GGUUUUACCA ACUGCGGCUA A UCUUUUUU UAUACUGAGC GUAUUGGAAC GUUAUCGAUA AGAAGAGAGC GUCUAGGCGA ACAAUGUUCU UAAA

GENBANK: GENBANK: KY203910.1

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分子 #4: Ribosome biogenesis protein RLP7

分子名称: Ribosome biogenesis protein RLP7 / タイプ: protein_or_peptide / ID: 4 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 36.621074 KDa
配列文字列: MSSTQDSKAQ TLNSNPEILL RKRRNADRTR IERQELAKKK REEQIKKKRS NKNKFVRAES IVAKTLATSR EKERIKRVSI LEDKKAKNE TQHIASGKDF ILKITEKANG AEENSVDLEE TEEEEDDGLI REKTTYDGKP ALLFIVRVRG PLAVNIPNKA F KILSLLRL ...文字列:
MSSTQDSKAQ TLNSNPEILL RKRRNADRTR IERQELAKKK REEQIKKKRS NKNKFVRAES IVAKTLATSR EKERIKRVSI LEDKKAKNE TQHIASGKDF ILKITEKANG AEENSVDLEE TEEEEDDGLI REKTTYDGKP ALLFIVRVRG PLAVNIPNKA F KILSLLRL VETNTGVFVK LTKNVYPLLK VIAPYVVIGK PSLSSIRSLI QKRGRIIYKG ENEAEPHEIV LNDNNIVEEQ LG DHGIICV EDIIHEIATM GESFSVCNFF LQPFKLNREV SGFGSLNRLR KIKQREAESR TRQFSNAATA PVIEVDIDSL LAK LN

UniProtKB: Ribosome biogenesis protein RLP7

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分子 #5: Ribosome biogenesis protein 15

分子名称: Ribosome biogenesis protein 15 / タイプ: protein_or_peptide / ID: 5 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 25.499186 KDa
配列文字列: MVKSTSKTST KETVTKQPTE EKPIQEKEEL ALETSSSSSD EEDEKDEDEI EGLAASDDEQ SGTHKIKRLN PKKQANEKKS KDKKTLEEY SGIIYVSRLP HGFHEKELSK YFAQFGDLKE VRLARNKKTG NSRHYGFLEF VNKEDAMIAQ ESMNNYLLMG H LLQVRVLP ...文字列:
MVKSTSKTST KETVTKQPTE EKPIQEKEEL ALETSSSSSD EEDEKDEDEI EGLAASDDEQ SGTHKIKRLN PKKQANEKKS KDKKTLEEY SGIIYVSRLP HGFHEKELSK YFAQFGDLKE VRLARNKKTG NSRHYGFLEF VNKEDAMIAQ ESMNNYLLMG H LLQVRVLP KGAKIEKLYK YKKRVLVEKG ITKPVKQLKD NMKQKHEERI KKLAKSGIEF KW

UniProtKB: Ribosome biogenesis protein 15

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分子 #6: 60S ribosomal protein L3

分子名称: 60S ribosomal protein L3 / タイプ: protein_or_peptide / ID: 6 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 43.850793 KDa
配列文字列: MSHRKYEAPR HGHLGFLPRK RAASIRARVK AFPKDDRSKP VALTSFLGYK AGMTTIVRDL DRPGSKFHKR EVVEAVTVVD TPPVVVVGV VGYVETPRGL RSLTTVWAEH LSDEVKRRFY KNWYKSKKKA FTKYSAKYAQ DGAGIERELA RIKKYASVVR V LVHTQIRK ...文字列:
MSHRKYEAPR HGHLGFLPRK RAASIRARVK AFPKDDRSKP VALTSFLGYK AGMTTIVRDL DRPGSKFHKR EVVEAVTVVD TPPVVVVGV VGYVETPRGL RSLTTVWAEH LSDEVKRRFY KNWYKSKKKA FTKYSAKYAQ DGAGIERELA RIKKYASVVR V LVHTQIRK TPLAQKKAHL AEIQLNGGSI SEKVDWAREH FEKTVAVDSV FEQNEMIDAI AVTKGHGFEG VTHRWGTKKL PR KTHRGLR KVACIGAWHP AHVMWSVARA GQRGYHSRTS INHKIYRVGK GDDEANGATS FDRTKKTITP MGGFVHYGEI KND FIMVKG CIPGNRKRIV TLRKSLYTNT SRKALEEVSL KWIDTASKFG KGRFQTPAEK HAFMGTLKKD L

UniProtKB: Large ribosomal subunit protein uL3

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分子 #7: 60S ribosomal protein L4-A

分子名称: 60S ribosomal protein L4-A / タイプ: protein_or_peptide / ID: 7 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 39.159125 KDa
配列文字列: MSRPQVTVHS LTGEATANAL PLPAVFSAPI RPDIVHTVFT SVNKNKRQAY AVSEKAGHQT SAESWGTGRA VARIPRVGGG GTGRSGQGA FGNMCRGGRM FAPTKTWRKW NVKVNHNEKR YATASAIAAT AVASLVLARG HRVEKIPEIP LVVSTDLESI Q KTKEAVAA ...文字列:
MSRPQVTVHS LTGEATANAL PLPAVFSAPI RPDIVHTVFT SVNKNKRQAY AVSEKAGHQT SAESWGTGRA VARIPRVGGG GTGRSGQGA FGNMCRGGRM FAPTKTWRKW NVKVNHNEKR YATASAIAAT AVASLVLARG HRVEKIPEIP LVVSTDLESI Q KTKEAVAA LKAVGAHSDL LKVLKSKKLR AGKGKYRNRR WTQRRGPLVV YAEDNGIVKA LRNVPGVETA NVASLNLLQL AP GAHLGRF VIWTEAAFTK LDQVWGSETV ASSKVGYTLP SHIISTSDVT RIINSSEIQS AIRPAGQATQ KRTHVLKKNP LKN KQVLLR LNPYAKVFAA EKLGSKKAEK TGTKPAAVFT ETLKHD

UniProtKB: Large ribosomal subunit protein uL4A

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分子 #8: Proteasome-interacting protein CIC1

分子名称: Proteasome-interacting protein CIC1 / タイプ: protein_or_peptide / ID: 8 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 42.596691 KDa
配列文字列: MAKKSNSKKS TPVSTPSKEK KKVIEKKSST AIPRERVIKA VNELIKFTSK PQDENNEEGN NGKKNLLEDD EEELKKDLQL IVVNNKSFT GTSKSFKLKL LNVKHSFYKP WKEASATAVK DFKVLLILKD SDIKKVSEDD LFDQLDSEGI KVDEIICGKD L KTVYKAYE ...文字列:
MAKKSNSKKS TPVSTPSKEK KKVIEKKSST AIPRERVIKA VNELIKFTSK PQDENNEEGN NGKKNLLEDD EEELKKDLQL IVVNNKSFT GTSKSFKLKL LNVKHSFYKP WKEASATAVK DFKVLLILKD SDIKKVSEDD LFDQLDSEGI KVDEIICGKD L KTVYKAYE ARNAFISQFS LILADDSIVT SLPKLMGGKA YNKVETTPIS IRTHANKEFS LTTLTNNIKK VYMNQLPVKL PR GTTLNVH LGNLEWLRPE EFVDNVELIS EQLIKAYQIR SIFIKTNRSP VLPLYYNQDV LDELEAKKDK IEETHEDDMV TID GVQVHL STFNKGLMEI ANPSELGSIF SKQINNAKKR SSSELEKESS ESEAVKKAKS

UniProtKB: Proteasome-interacting protein CIC1

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分子 #9: 60S ribosomal protein L6-A

分子名称: 60S ribosomal protein L6-A / タイプ: protein_or_peptide / ID: 9 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 20.000564 KDa
配列文字列:
MSAQKAPKWY PSEDVAALKK TRKAARPQKL RASLVPGTVL ILLAGRFRGK RVVYLKHLED NTLLISGPFK VNGVPLRRVN ARYVIATST KVSVEGVNVE KFNVEYFAKE KLTKKEKKEA NLFPEQQNKE IKAERVEDQK VVDKALIAEI KKTPLLKQYL S ASFSLKNG DKPHMLKF

UniProtKB: Large ribosomal subunit protein eL6A

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分子 #10: 60S ribosomal protein L7-A

分子名称: 60S ribosomal protein L7-A / タイプ: protein_or_peptide / ID: 10 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 27.686281 KDa
配列文字列: MAAEKILTPE SQLKKSKAQQ KTAEQVAAER AARKAANKEK RAIILERNAA YQKEYETAER NIIQAKRDAK AAGSYYVEAQ HKLVFVVRI KGINKIPPKP RKVLQLLRLT RINSGTFVKV TKATLELLKL IEPYVAYGYP SYSTIRQLVY KRGFGKINKQ R VPLSDNAI ...文字列:
MAAEKILTPE SQLKKSKAQQ KTAEQVAAER AARKAANKEK RAIILERNAA YQKEYETAER NIIQAKRDAK AAGSYYVEAQ HKLVFVVRI KGINKIPPKP RKVLQLLRLT RINSGTFVKV TKATLELLKL IEPYVAYGYP SYSTIRQLVY KRGFGKINKQ R VPLSDNAI IEANLGKYGI LSIDDLIHEI ITVGPHFKQA NNFLWPFKLS NPSGGWGVPR KFKHFIQGGS FGNREEFINK LV KSMN

UniProtKB: Large ribosomal subunit protein uL30A

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分子 #11: 60S ribosomal protein L8-A

分子名称: 60S ribosomal protein L8-A / タイプ: protein_or_peptide / ID: 11 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 28.17582 KDa
配列文字列: MAPGKKVAPA PFGAKSTKSN KTRNPLTHST PKNFGIGQAV QPKRNLSRYV KWPEYVRVQR QKKILSIRLK VPPTIAQFQY TLDRNTAAE TFKLFNKYRP ETAAEKKERL TKEAAAVAEG KSKQDASPKP YAVKYGLNHV VALIENKKAK LVLIANDVDP I ELVVFLPA ...文字列:
MAPGKKVAPA PFGAKSTKSN KTRNPLTHST PKNFGIGQAV QPKRNLSRYV KWPEYVRVQR QKKILSIRLK VPPTIAQFQY TLDRNTAAE TFKLFNKYRP ETAAEKKERL TKEAAAVAEG KSKQDASPKP YAVKYGLNHV VALIENKKAK LVLIANDVDP I ELVVFLPA LCKKMGVPYA IVKGKARLGT LVNQKTSAVA ALTEVRAEDE AALAKLVSTI DANFADKYDE VKKHWGGGIL GN KAQAKMD KRAKNSDSA

UniProtKB: Large ribosomal subunit protein eL8A

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分子 #12: 60S ribosomal protein L9-A

分子名称: 60S ribosomal protein L9-A / タイプ: protein_or_peptide / ID: 12 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 21.605061 KDa
配列文字列: MKYIQTEQQI EVPEGVTVSI KSRIVKVVGP RGTLTKNLKH IDVTFTKVNN QLIKVAVHNG GRKHVAALRT VKSLVDNMIT GVTKGYKYK MRYVYAHFPI NVNIVEKDGA KFIEVRNFLG DKKIRNVPVR DGVTIEFSTN VKDEIVLSGN SVEDVSQNAA D LQQICRVR ...文字列:
MKYIQTEQQI EVPEGVTVSI KSRIVKVVGP RGTLTKNLKH IDVTFTKVNN QLIKVAVHNG GRKHVAALRT VKSLVDNMIT GVTKGYKYK MRYVYAHFPI NVNIVEKDGA KFIEVRNFLG DKKIRNVPVR DGVTIEFSTN VKDEIVLSGN SVEDVSQNAA D LQQICRVR NKDIRKFLDG IYVSHKGFIT EDL

UniProtKB: Large ribosomal subunit protein uL6A

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分子 #13: Nucleolar protein 7

分子名称: Nucleolar protein 7 / タイプ: protein_or_peptide / ID: 13 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 69.984148 KDa
配列文字列: MRIKKKNTRG NARNFITRSQ AVRKLQVSLA DFRRLCIFKG IYPREPRNKK KANKGSTAPT TFYYAKDIQY LMHEPVLAKF REHKTFARK LTRALGRGEV SSAKRLEENR DSYTLDHIIK ERYPSFPDAI RDIDDALNML FLFSNLPSTN QVSSKIINDA Q KICNQWLA ...文字列:
MRIKKKNTRG NARNFITRSQ AVRKLQVSLA DFRRLCIFKG IYPREPRNKK KANKGSTAPT TFYYAKDIQY LMHEPVLAKF REHKTFARK LTRALGRGEV SSAKRLEENR DSYTLDHIIK ERYPSFPDAI RDIDDALNML FLFSNLPSTN QVSSKIINDA Q KICNQWLA YVAKERLVRK VFVSIKGVYY QANIKGEEVR WLVPFKFPEN IPSDVDFRIM LTFLEFYSTL LHFVLYKLYT DS GLIYPPK LDLKKDKIIS GLSSYILESR QEDSLLKLDP TEIEEDVKVE SLDASTLKSA LNADEANTDE TEKEEEQEKK QEK EQEKEQ NEETELDTFE DNNKNKGDIL IQPSKYDSPV ASLFSAFVFY VSREVPIDIL EFLILSCGGN VISEAAMDQI ENKK DIDMS KVTHQIVDRP VLKNKVAGRT YIQPQWIFDC INKGELVPAN KYLPGEALPP HLSPWGDAIG YDPTAPVEEG EEEES ESES ESEDQVEEED QEVVAGEEDD DDDEELQAQK ELELEAQGIK YSETSEADKD VNKSKNKKRK VDEEEEEKKL KMIMMS NKQ KKLYKKMKYS NAKKEEQAEN LKKKKKQIAK QKAKLNKLDS KK

UniProtKB: Pescadillo homolog

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分子 #14: Eukaryotic translation initiation factor 6

分子名称: Eukaryotic translation initiation factor 6 / タイプ: protein_or_peptide / ID: 14 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 26.476605 KDa
配列文字列: MATRTQFENS NEIGVFSKLT NTYCLVAVGG SENFYSAFEA ELGDAIPIVH TTIAGTRIIG RMTAGNRRGL LVPTQTTDQE LQHLRNSLP DSVKIQRVEE RLSALGNVIC CNDYVALVHP DIDRETEELI SDVLGVEVFR QTISGNILVG SYCSLSNQGG L VHPQTSVQ ...文字列:
MATRTQFENS NEIGVFSKLT NTYCLVAVGG SENFYSAFEA ELGDAIPIVH TTIAGTRIIG RMTAGNRRGL LVPTQTTDQE LQHLRNSLP DSVKIQRVEE RLSALGNVIC CNDYVALVHP DIDRETEELI SDVLGVEVFR QTISGNILVG SYCSLSNQGG L VHPQTSVQ DQEELSSLLQ VPLVAGTVNR GSSVVGAGMV VNDYLAVTGL DTTAPELSVI ESIFRLQDAQ PESISGNLRD TL IETYS

UniProtKB: Eukaryotic translation initiation factor 6

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分子 #15: Ribosome production factor 1

分子名称: Ribosome production factor 1 / タイプ: protein_or_peptide / ID: 15 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 35.184387 KDa
配列文字列: MALGNEINIT NKLKRQEIFA DIKHEKNKER HTMRRKRAKE ERENPELREQ RLKENVTQTI ENTRVYDETI NKEVEGDEDD LMRYFNSNS NEPPKIFLTT NVNAKKSAYE FANILIEILP NVTFVKRKFG YKLKEISDIC IKRNFTDIVI INEDKKKVTG L TFIHLPEG ...文字列:
MALGNEINIT NKLKRQEIFA DIKHEKNKER HTMRRKRAKE ERENPELREQ RLKENVTQTI ENTRVYDETI NKEVEGDEDD LMRYFNSNS NEPPKIFLTT NVNAKKSAYE FANILIEILP NVTFVKRKFG YKLKEISDIC IKRNFTDIVI INEDKKKVTG L TFIHLPEG PTFYFKLSSF VEVKKIVGHG RPTSHIPELI LNNFQTRLGQ TVGRLFQSIL PQNPDIEGRQ VITLHNQRDY IF FRRHRYV FKDNERVGLQ ELGPQFTLKL KRLQRGIKEE TEWEHKPEMD KEKKKFYL

UniProtKB: Ribosome production factor 1

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分子 #16: 60S ribosomal protein L13-A

分子名称: 60S ribosomal protein L13-A / タイプ: protein_or_peptide / ID: 16 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 22.604164 KDa
配列文字列: MAISKNLPIL KNHFRKHWQE RVKVHFDQAG KKVSRRNARA TRAAKIAPRP LDLLRPVVRA PTVKYNRKVR AGRGFTLAEV KAAGLTAAY ARTIGIAVDH RRQNRNQEIF DANVQRLKEY QSKIIVFPRN GKAPEAEQVL SAAATFPIAQ PATDVEARAV Q DNGESAFR ...文字列:
MAISKNLPIL KNHFRKHWQE RVKVHFDQAG KKVSRRNARA TRAAKIAPRP LDLLRPVVRA PTVKYNRKVR AGRGFTLAEV KAAGLTAAY ARTIGIAVDH RRQNRNQEIF DANVQRLKEY QSKIIVFPRN GKAPEAEQVL SAAATFPIAQ PATDVEARAV Q DNGESAFR TLRLARSEKK FRGIREKRAR EKAEAEAEKK K

UniProtKB: Large ribosomal subunit protein eL13A

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分子 #17: 60S ribosomal protein L14-A

分子名称: 60S ribosomal protein L14-A / タイプ: protein_or_peptide / ID: 17 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 15.195066 KDa
配列文字列:
MSTDSIVKAS NWRLVEVGRV VLIKKGQSAG KLAAIVEIID QKKVLIDGPK AGVPRQAINL GQVVLTPLTF ALPRGARTAT VSKKWAAAA VCEKWAASSW AKKIAQRERR AALTDFERFQ VMVLRKQKRY TVKKALAKA

UniProtKB: Large ribosomal subunit protein eL14A

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分子 #18: 60S ribosomal protein L15-A

分子名称: 60S ribosomal protein L15-A / タイプ: protein_or_peptide / ID: 18 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 24.482357 KDa
配列文字列: MGAYKYLEEL QRKKQSDVLR FLQRVRVWEY RQKNVIHRAA RPTRPDKARR LGYKAKQGFV IYRVRVRRGN RKRPVPKGAT YGKPTNQGV NELKYQRSLR ATAEERVGRR AANLRVLNSY WVNQDSTYKY FEVILVDPQH KAIRRDARYN WICDPVHKHR E ARGLTATG ...文字列:
MGAYKYLEEL QRKKQSDVLR FLQRVRVWEY RQKNVIHRAA RPTRPDKARR LGYKAKQGFV IYRVRVRRGN RKRPVPKGAT YGKPTNQGV NELKYQRSLR ATAEERVGRR AANLRVLNSY WVNQDSTYKY FEVILVDPQH KAIRRDARYN WICDPVHKHR E ARGLTATG KKSRGINKGH KFNNTKAGRR KTWKRQNTLS LWRYRK

UniProtKB: Large ribosomal subunit protein eL15A

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分子 #19: 60S ribosomal protein L16-A

分子名称: 60S ribosomal protein L16-A / タイプ: protein_or_peptide / ID: 19 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 22.247227 KDa
配列文字列: MSVEPVVVID GKGHLVGRLA SVVAKQLLNG QKIVVVRAEE LNISGEFFRN KLKYHDFLRK ATAFNKTRGP FHFRAPSRIF YKALRGMVS HKTARGKAAL ERLKVFEGIP PPYDKKKRVV VPQALRVLRL KPGRKYTTLG KLSTSVGWKY EDVVAKLEAK R KVSSAEYY ...文字列:
MSVEPVVVID GKGHLVGRLA SVVAKQLLNG QKIVVVRAEE LNISGEFFRN KLKYHDFLRK ATAFNKTRGP FHFRAPSRIF YKALRGMVS HKTARGKAAL ERLKVFEGIP PPYDKKKRVV VPQALRVLRL KPGRKYTTLG KLSTSVGWKY EDVVAKLEAK R KVSSAEYY AKKRAFTKKV ASANATAAES DVAKQLAALG Y

UniProtKB: Large ribosomal subunit protein uL13A

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分子 #20: 60S ribosomal protein L17-A

分子名称: 60S ribosomal protein L17-A / タイプ: protein_or_peptide / ID: 20 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 20.589518 KDa
配列文字列:
MARYGATSTN PAKSASARGS YLRVSFKNTR ETAQAINGWE LTKAQKYLEQ VLDHQRAIPF RRFNSSIGRT AQGKEFGVTK ARWPAKSVK FVQGLLQNAA ANAEAKGLDA TKLYVSHIQV NQAPKQRRRT YRAHGRINKY ESSPSHIELV VTEKEEAVAK A AEKKVVRL TSRQRGRIAA QKRIAA

UniProtKB: Large ribosomal subunit protein uL22A

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分子 #21: 60S ribosomal protein L18-A

分子名称: 60S ribosomal protein L18-A / タイプ: protein_or_peptide / ID: 21 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 20.609252 KDa
配列文字列:
MGIDHTSKQH KRSGHRTAPK SDNVYLKLLV KLYTFLARRT DAPFNKVVLK ALFLSKINRP PVSVSRIARA LKQEGAANKT VVVVGTVTD DARIFEFPKT TVAALRFTAG ARAKIVKAGG ECITLDQLAV RAPKGQNTLI LRGPRNSREA VRHFGMGPHK G KAPRILST GRKFERARGR RRSKGFKV

UniProtKB: Large ribosomal subunit protein eL18A

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分子 #22: Ribosome biogenesis protein NSA1

分子名称: Ribosome biogenesis protein NSA1 / タイプ: protein_or_peptide / ID: 22 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 51.982457 KDa
配列文字列: MRLLVSCVDS GSIKEVLCNI GTDTSVQSAL QPFHVAPHLA EGLKAYVDRM WVISEDEAIL ARNSGVVELV KISKHLKENE ALQVDPKGE SKNEKSLSDD LPKFDISEFE ITSSVSDLFD DAKLESLSSK SVKRTKLVDG FVTLCPIKKD SSNNTFVAAT K SGLLHIIK ...文字列:
MRLLVSCVDS GSIKEVLCNI GTDTSVQSAL QPFHVAPHLA EGLKAYVDRM WVISEDEAIL ARNSGVVELV KISKHLKENE ALQVDPKGE SKNEKSLSDD LPKFDISEFE ITSSVSDLFD DAKLESLSSK SVKRTKLVDG FVTLCPIKKD SSNNTFVAAT K SGLLHIIK KGEDKKLIKL ASLGLKAPVE FLQLYDLEDT DTDKYIFAYG GEENLIKLVE IDSSFQSLKQ IWEAKNVKND RL DMRVPVW PMALRFLEPS PGKTEKGKLN YQFAAITRWS HLTKYSTQHG RKPFAQIDLL PNREPLSQME VFDAKGENVV SSL GNFQSE TFNELNVITT DYKKNVFKFD GNGRMLGKVG RDDITGSSTY IHVHDGKYLL QGGLDRYVRI FDIKTNKMLV KVYV GSRIN FIVMLDDVEI EMPLSPSAKA AKGKQKRKVT ELEEDADELW NKLEGKVAAS KASKKSKI

UniProtKB: Ribosome biogenesis protein NSA1

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分子 #23: 60S ribosomal protein L20-A

分子名称: 60S ribosomal protein L20-A / タイプ: protein_or_peptide / ID: 23 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 20.478852 KDa
配列文字列:
MAHFKEYQVI GRRLPTESVP EPKLFRMRIF ASNEVIAKSR YWYFLQKLHK VKKASGEIVS INQINEAHPT KVKNFGVWVR YDSRSGTHN MYKEIRDVSR VAAVETLYQD MAARHRARFR SIHILKVAEI EKTADVKRQY VKQFLTKDLK FPLPHRVQKS T KTFSYKRP STFY

UniProtKB: Large ribosomal subunit protein eL20A

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分子 #24: Ribosomal RNA-processing protein 1

分子名称: Ribosomal RNA-processing protein 1 / タイプ: protein_or_peptide / ID: 24 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 33.250531 KDa
配列文字列: METSNFVKQL SSNNRKTRVN ALEALKKYLT AKQFKENKQI EFNKLWKGLY YAMWFSDRPR PQQRLANELG ELHGLYFDPK DNSTADELT TNDKAFIKFS RGFWKVMCFE WFNIDRYRLD KYLLLIRRVL FSQLKYLQSR NWDKKLVDEY IKKVLRWLPL S GSPKVYTG ...文字列:
METSNFVKQL SSNNRKTRVN ALEALKKYLT AKQFKENKQI EFNKLWKGLY YAMWFSDRPR PQQRLANELG ELHGLYFDPK DNSTADELT TNDKAFIKFS RGFWKVMCFE WFNIDRYRLD KYLLLIRRVL FSQLKYLQSR NWDKKLVDEY IKKVLRWLPL S GSPKVYTG IPIHIVDILL DEWERLLKDG DEDDEDEENK EEEMRKIAES AKKTPLADVI AIFQDIVADY NNSKVLREKI KE DLFSDTR LVSWDILEGE TQHNDSSNES EEEEEEEWKG F

UniProtKB: Ribosomal RNA-processing protein 1

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分子 #25: ATP-dependent RNA helicase HAS1

分子名称: ATP-dependent RNA helicase HAS1 / タイプ: protein_or_peptide / ID: 25 / コピー数: 1 / 光学異性体: LEVO / EC番号: RNA helicase
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 56.798348 KDa
配列文字列: MATPSNKRSR DSESTEEPVV DEKSTSKQNN AAPEGEQTTC VEKFEELKLS QPTLKAIEKM GFTTMTSVQA RTIPPLLAGR DVLGAAKTG SGKTLAFLIP AIELLHSLKF KPRNGTGIIV ITPTRELALQ IFGVARELME FHSQTFGIVI GGANRRQEAE K LMKGVNML ...文字列:
MATPSNKRSR DSESTEEPVV DEKSTSKQNN AAPEGEQTTC VEKFEELKLS QPTLKAIEKM GFTTMTSVQA RTIPPLLAGR DVLGAAKTG SGKTLAFLIP AIELLHSLKF KPRNGTGIIV ITPTRELALQ IFGVARELME FHSQTFGIVI GGANRRQEAE K LMKGVNML IATPGRLLDH LQNTKGFVFK NLKALIIDEA DRILEIGFED EMRQIIKILP NEDRQSMLFS ATQTTKVEDL AR ISLRPGP LFINVVPETD NSTADGLEQG YVVCDSDKRF LLLFSFLKRN QKKKIIVFLS SCNSVKYYAE LLNYIDLPVL ELH GKQKQQ KRTNTFFEFC NAERGILICT DVAARGLDIP AVDWIIQFDP PDDPRDYIHR VGRTARGTKG KGKSLMFLTP NELG FLRYL KASKVPLNEY EFPENKIANV QSQLEKLIKS NYYLHQTAKD GYRSYLQAYA SHSLKTVYQI DKLDLAKVAK SYGFP VPPK VNITIGASGK TPNTKRRKTH K

UniProtKB: ATP-dependent RNA helicase HAS1

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分子 #26: Protein MAK16

分子名称: Protein MAK16 / タイプ: protein_or_peptide / ID: 26 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 35.754605 KDa
配列文字列: MSDEIVWQVI NQSFCSHRIK APNGQNFCRN EYNVTGLCTR QSCPLANSKY ATVKCDNGKL YLYMKTPERA HTPAKLWERI KLSKNYTKA LQQIDEHLLH WSKFFRHKCK QRFTKLTQVM ITERRLALRE EERHYVGVAP KVKRREQNRE RKALVAAKIE K AIEKELMD ...文字列:
MSDEIVWQVI NQSFCSHRIK APNGQNFCRN EYNVTGLCTR QSCPLANSKY ATVKCDNGKL YLYMKTPERA HTPAKLWERI KLSKNYTKA LQQIDEHLLH WSKFFRHKCK QRFTKLTQVM ITERRLALRE EERHYVGVAP KVKRREQNRE RKALVAAKIE K AIEKELMD RLKSGAYGDK PLNVDEKVWK KIMGQMEEEN SQDEEEDWDE EEESDDGEVE YVADDGEGEY VDVDDLEKWL AD SDREASS ASESESDSES ESDSDSDEEN KNSAKRRKKG TSAKTKRPKV EIEYEEEHEV QNAEQEVAQ

UniProtKB: Protein MAK16

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分子 #27: Ribosome biogenesis protein BRX1

分子名称: Ribosome biogenesis protein BRX1 / タイプ: protein_or_peptide / ID: 27 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 33.585414 KDa
配列文字列: MSSIYKALAG KSKDNKSEKK QGNVKQFMNK QRTLLISSRG VNYRHRHLIQ DLSGLLPHSR KEPKLDTKKD LQQLNEIAEL YNCNNVLFF EARKHQDLYL WLSKPPNGPT IKFYIQNLHT MDELNFTGNC LKGSRPVLSF DQRFESSPHY QLIKELLVHN F GVPPNARK ...文字列:
MSSIYKALAG KSKDNKSEKK QGNVKQFMNK QRTLLISSRG VNYRHRHLIQ DLSGLLPHSR KEPKLDTKKD LQQLNEIAEL YNCNNVLFF EARKHQDLYL WLSKPPNGPT IKFYIQNLHT MDELNFTGNC LKGSRPVLSF DQRFESSPHY QLIKELLVHN F GVPPNARK SKPFIDHVMS FSIVDDKIWV RTYEISHSTK NKEEYEDGEE DISLVEIGPR FVMTVILILE GSFGGPKIYE NK QYVSPNV VRAQIKQQAA EEAKSRAEAA VERKIKRREN VLAADPLSND ALFK

UniProtKB: Ribosome biogenesis protein BRX1

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分子 #28: rRNA-processing protein EBP2

分子名称: rRNA-processing protein EBP2 / タイプ: protein_or_peptide / ID: 28 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 49.842613 KDa
配列文字列: MAKGFKLKEL LSHQKEIEKA EKLENDLKKK KSQELKKEEP TIVTASNLKK LEKKEKKADV KKEVAADTEE YQSQALSKKE KRKLKKELK KMQEQDATEA QKHMSGDEDE SGDDREEEEE EEEEEEGRLD LEKLAKSDSE SEDDSESEND SEEDEDVVAK E ESEEKEEQ ...文字列:
MAKGFKLKEL LSHQKEIEKA EKLENDLKKK KSQELKKEEP TIVTASNLKK LEKKEKKADV KKEVAADTEE YQSQALSKKE KRKLKKELK KMQEQDATEA QKHMSGDEDE SGDDREEEEE EEEEEEGRLD LEKLAKSDSE SEDDSESEND SEEDEDVVAK E ESEEKEEQ EEEQDVPLSD VEFDSDADVV PHHKLTVNNT KAMKHALERV QLPWKKHSFQ EHQSVTSETN TDEHIKDIYD DT ERELAFY KQSLDAVLVA RDELKRLKVP FKRPLDYFAE MVKSDEHMDK IKGKLIEEAS DKKAREEARR QRQLKKFGKQ VQN ATLQKR QLEKRETLEK IKSLKNKRKH NEIDHSEFNV GVEEEVEGKR FDRGRPNGKR AAKNAKYGQG GMKRFKRKND ATSS ADVSG FSSRKMKGKT NRPGKSRRAR RF

UniProtKB: rRNA-processing protein EBP2

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分子 #29: 60S ribosomal protein L26-A

分子名称: 60S ribosomal protein L26-A / タイプ: protein_or_peptide / ID: 29 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 14.265784 KDa
配列文字列:
MAKQSLDVSS DRRKARKAYF TAPSSQRRVL LSAPLSKELR AQYGIKALPI RRDDEVLVVR GSKKGQEGKI SSVYRLKFAV QVDKVTKEK VNGASVPINL HPSKLVITKL HLDKDRKALI QRKGGKLE

UniProtKB: Large ribosomal subunit protein uL24A

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分子 #30: Unassigned

分子名称: Unassigned / タイプ: protein_or_peptide / ID: 30 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae (パン酵母)
分子量理論値: 13.12417 KDa
配列文字列: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) ...文字列:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)

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分子 #31: 60S ribosomal protein L32

分子名称: 60S ribosomal protein L32 / タイプ: protein_or_peptide / ID: 31 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 14.809441 KDa
配列文字列:
MASLPHPKIV KKHTKKFKRH HSDRYHRVAE NWRKQKGIDS VVRRRFRGNI SQPKIGYGSN KKTKFLSPSG HKTFLVANVK DLETLTMHT KTYAAEIAHN ISAKNRVVIL ARAKALGIKV TNPKGRLALE A

UniProtKB: Large ribosomal subunit protein eL32

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分子 #32: 60S ribosomal protein L33-A

分子名称: 60S ribosomal protein L33-A / タイプ: protein_or_peptide / ID: 32 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 12.17713 KDa
配列文字列:
MAESHRLYVK GKHLSYQRSK RVNNPNVSLI KIEGVATPQD AQFYLGKRIA YVYRASKEVR GSKIRVMWGK VTRTHGNSGV VRATFRNNL PAKTFGASVR IFLYPSNI

UniProtKB: Large ribosomal subunit protein eL33A

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分子 #33: 60S ribosomal protein L35-A

分子名称: 60S ribosomal protein L35-A / タイプ: protein_or_peptide / ID: 33 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 13.94264 KDa
配列文字列:
MAGVKAYELR TKSKEQLASQ LVDLKKELAE LKVQKLSRPS LPKIKTVRKS IACVLTVINE QQREAVRQLY KGKKYQPKDL RAKKTRALR RALTKFEASQ VTEKQRKKQI AFPQRKYAIK A

UniProtKB: Large ribosomal subunit protein uL29A

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分子 #34: 60S ribosomal protein L36-A

分子名称: 60S ribosomal protein L36-A / タイプ: protein_or_peptide / ID: 34 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 11.151259 KDa
配列文字列:
MTVKTGIAIG LNKGKKVTSM TPAPKISYKK GAASNRTKFV RSLVREIAGL SPYERRLIDL IRNSGEKRAR KVAKKRLGSF TRAKAKVEE MNNIIAASRR H

UniProtKB: Large ribosomal subunit protein eL36A

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分子 #35: 60S ribosomal protein L37-A

分子名称: 60S ribosomal protein L37-A / タイプ: protein_or_peptide / ID: 35 / コピー数: 1 / 光学異性体: LEVO
由来(天然)生物種: Saccharomyces cerevisiae S288c (パン酵母) / 株: ATCC 204508 / S288c
分子量理論値: 9.877395 KDa
配列文字列:
MGKGTPSFGK RHNKSHTLCN RCGRRSFHVQ KKTCSSCGYP AAKTRSYNWG AKAKRRHTTG TGRMRYLKHV SRRFKNGFQT GSASKASA

UniProtKB: Large ribosomal subunit protein eL37A

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実験情報

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構造解析

手法クライオ電子顕微鏡法
解析単粒子再構成法
試料の集合状態particle

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試料調製

緩衝液pH: 8
構成要素:
濃度名称式
10.0 mMTris-HCl
100.0 mMsodium chlorideNaCl
2.0 mMDTT
グリッド材質: COPPER / メッシュ: 400 / 支持フィルム - 材質: CARBON / 支持フィルム - トポロジー: HOLEY / 前処理 - タイプ: GLOW DISCHARGE / 前処理 - 時間: 60 sec. / 前処理 - 雰囲気: AIR
凍結凍結剤: ETHANE / チャンバー内湿度: 100 % / チャンバー内温度: 277 K / 装置: FEI VITROBOT MARK IV / 詳細: blot for 1-3 seconds before plunging.

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電子顕微鏡法

顕微鏡FEI TITAN KRIOS
撮影フィルム・検出器のモデル: FEI FALCON III (4k x 4k)
撮影したグリッド数: 1 / 実像数: 1983 / 平均露光時間: 1.5 sec. / 平均電子線量: 30.0 e/Å2
電子線加速電圧: 300 kV / 電子線源: FIELD EMISSION GUN
電子光学系照射モード: FLOOD BEAM / 撮影モード: BRIGHT FIELD / Cs: 2.7 mm / 最大 デフォーカス（公称値）: 4.0 µm / 最小 デフォーカス（公称値）: 1.0 µm
試料ステージ試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER
ホルダー冷却材: NITROGEN
実験機器
モデル: Titan Krios / 画像提供: FEI Company

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画像解析

粒子像選択選択した数: 331816
初期モデルモデルのタイプ: PDB ENTRY
PDBモデル - PDB ID:

4v88


詳細: 40S ribosome
最終 再構成想定した対称性 - 点群: C1 (非対称) / 解像度のタイプ: BY AUTHOR / 解像度: 3.65 Å / 解像度の算出法: FSC 0.143 CUT-OFF / ソフトウェア - 名称: RELION (ver. 1.4) / 使用した粒子像数: 98155
初期 角度割当タイプ: NOT APPLICABLE / ソフトウェア - 名称: RELION (ver. 1.4)
最終 角度割当タイプ: NOT APPLICABLE / ソフトウェア - 名称: RELION (ver. 1.4)
最終 3次元分類クラス数: 6 / ソフトウェア - 名称: RELION (ver. 1.4)
FSC曲線 (解像度の算出)

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原子モデル構築 1

詳細real space refinement
精密化空間: REAL
得られたモデル

PDB-5z3g:
Cryo-EM structure of a nucleolar pre-60S ribosome (Rpf1-TAP)

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万見について

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お知らせ

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2022年2月9日: EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

EMDBエントリの付随情報ファイルのフォーマットが新しくなりました

  • EMDBのヘッダファイルのバージョン3が、公式のフォーマットとなりました。
  • これまでは公式だったバージョン1.9は、アーカイブから削除されます。

関連情報:EMDBヘッダ

外部リンク:wwPDBはEMDBデータモデルのバージョン3へ移行します

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2020年8月12日: 新型コロナ情報

新型コロナ情報

URL: https://pdbj.org/emnavi/covid19.php

新ページ: EM Navigatorに新型コロナウイルスの特設ページを開設しました。

関連情報:Covid-19情報 / 2020年3月5日: 新型コロナウイルスの構造データ

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2020年3月5日: 新型コロナウイルスの構造データ

新型コロナウイルスの構造データ

関連情報:万見生物種 / 2020年8月12日: 新型コロナ情報

外部リンク:COVID-19特集ページ - PDBj / 今月の分子2020年2月：コロナウイルスプロテーアーゼ

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2019年1月31日: EMDBのIDの桁数の変更

EMDBのIDの桁数の変更

  • EMDBエントリに付与されているアクセスコード(EMDB-ID)は4桁の数字(例、EMD-1234)でしたが、間もなく枯渇します。これまでの4桁のID番号は4桁のまま変更されませんが、4桁の数字を使い切った後に発行されるIDは5桁以上の数字(例、EMD-12345)になります。5桁のIDは2019年の春頃から発行される見通しです。
  • EM Navigator/万見では、接頭語「EMD-」は省略されています。

関連情報:Q: 「EMD」とは何ですか？ / 万見/EM NavigatorにおけるID/アクセスコードの表記

外部リンク:EMDB Accession Codes are Changing Soon! / PDBjへお問い合わせ

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2017年7月12日: PDB大規模アップデート

PDB大規模アップデート

  • 新バージョンのPDBx/mmCIF辞書形式に基づくデータがリリースされました。
  • 今回の更新はバージョン番号が4から5になる大規模なもので、全エントリデータの書き換えが行われる「Remediation」というアップデートに該当します。
  • このバージョンアップで、電子顕微鏡の実験手法に関する多くの項目の書式が改定されました(例：em_softwareなど)。
  • EM NavigatorとYorodumiでも、この改定に基づいた表示内容になります。

外部リンク:wwPDB Remediation / OneDepデータ基準に準拠した、より強化された内容のモデル構造ファイルが、PDBアーカイブで公開されました。

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万見 (Yorodumi)

幾万の構造データを、幾万の視点から

  • 万見(Yorodumi)は、EMDB/PDB/SASBDBなどの構造データを閲覧するためのページです。
  • EM Navigatorの詳細ページの後継、Omokage検索のフロントエンドも兼ねています。

関連情報:EMDB / PDB / SASBDB / 3つのデータバンクの比較 / 万見検索 / 2016年8月31日: 新しいEM Navigatorと万見 / 万見文献 / Jmol/JSmol / 機能・相同性情報 / 新しいEM Navigatorと万見の変更点

他の情報も見る