分子量: 66198.094 Da / 分子数: 1 / 由来タイプ: 組換発現 詳細: Differences at the N-terminus arise from the vector used for expression (primarily a His tag). Differences at the C-terminus arise from the lack of electron density meaning this part could not be modelled. 由来: (組換発現) Thermotoga maritima (バクテリア) 遺伝子: galA / 発現宿主: Escherichia coli (大腸菌) 参照: UniProt: O33835, UniProt: G4FEF4*PLUS, alpha-galactosidase
温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 詳細: 0.2 M magnesium sulfate, 20% (w/v) PEG 3350 Crystal soaked in 1 mM inhibitor, 30% (w/v) PEG3350 for 16 hours prior to freezing. Temp details: Room temperature
解像度: 1.53→43.69 Å / Cor.coef. Fo:Fc: 0.965 / Cor.coef. Fo:Fc free: 0.955 / SU B: 2.4 / SU ML: 0.08 / 交差検証法: THROUGHOUT / ESU R: 0.079 / ESU R Free: 0.08 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.22233
4699
4.9 %
RANDOM
Rwork
0.19313
-
-
-
obs
0.19459
91173
98.21 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK