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- PDB-5l3o: Crystal Structure of Human Carbonic Anhydrase II in Complex with ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5l3o | ||||||||||||
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Title | Crystal Structure of Human Carbonic Anhydrase II in Complex with a Quinoline Oligoamide Foldamer | ||||||||||||
![]() | Carbonic anhydrase 2 | ||||||||||||
![]() | Lyase/Inhibitor / PROTEIN-FOLDAMER COMPLEX / PROTEIN FOLDAMER INTERACTIONS / MODIFIED INHIBITOR / ANCHORED FOLDAMER / HCAII DIMERISATION / QUINOLINE OLIGOAMIDE FOLDAMER / BENZENE SULFONAMIDE MODIFIED INHIBITOR / LYASE-INHIBITOR COMPLEX | ||||||||||||
Function / homology | ![]() positive regulation of cellular pH reduction / positive regulation of dipeptide transmembrane transport / regulation of monoatomic anion transport / secretion / cyanamide hydratase / cyanamide hydratase activity / arylesterase activity / regulation of chloride transport / Reversible hydration of carbon dioxide / angiotensin-activated signaling pathway ...positive regulation of cellular pH reduction / positive regulation of dipeptide transmembrane transport / regulation of monoatomic anion transport / secretion / cyanamide hydratase / cyanamide hydratase activity / arylesterase activity / regulation of chloride transport / Reversible hydration of carbon dioxide / angiotensin-activated signaling pathway / positive regulation of synaptic transmission, GABAergic / morphogenesis of an epithelium / regulation of intracellular pH / carbonic anhydrase / carbonate dehydratase activity / carbon dioxide transport / Erythrocytes take up oxygen and release carbon dioxide / Erythrocytes take up carbon dioxide and release oxygen / neuron cellular homeostasis / one-carbon metabolic process / apical part of cell / myelin sheath / extracellular exosome / zinc ion binding / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||||||||
Biological species | ![]() | ||||||||||||
Method | ![]() ![]() ![]() ![]() | ||||||||||||
![]() | Jewginski, M. / Langlois d'Estaintot, B. / Granier, T. / Huc, Y. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Self-Assembled Protein-Aromatic Foldamer Complexes with 2:3 and 2:2:1 Stoichiometries. Authors: Jewginski, M. / Granier, T. / Langlois d'Estaintot, B. / Fischer, L. / Mackereth, C.D. / Huc, I. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 238.6 KB | Display | ![]() |
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PDB format | ![]() | 191.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5l6kC ![]() 5lvsC ![]() 3ks3S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: _ / Ens-ID: 1 / Beg auth comp-ID: SER / Beg label comp-ID: SER / End auth comp-ID: LYS / End label comp-ID: LYS / Refine code: _ / Auth seq-ID: 2 - 260 / Label seq-ID: 2 - 260
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 29289.062 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Non-polymers , 8 types, 289 molecules ![](data/chem/img/ZN.gif)
![](data/chem/img/6H0.gif)
![](data/chem/img/QUK.gif)
![](data/chem/img/QVS.gif)
![](data/chem/img/QVE.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/QUJ.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/6H0.gif)
![](data/chem/img/QUK.gif)
![](data/chem/img/QVS.gif)
![](data/chem/img/QVE.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/QUJ.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | #3: Chemical | ChemComp-6H0 / ~{ #4: Chemical | ChemComp-QUK / #5: Chemical | ChemComp-QVS / #6: Chemical | ChemComp-QVE / #7: Chemical | ChemComp-GOL / #8: Chemical | ChemComp-QUJ / #9: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.91 Å3/Da / Density % sol: 57.66 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 4.6 / Details: NA acetate, PEG 4000, NaN3 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Mar 13, 2015 / Details: Pt coated Si mirrors |
Radiation | Monochromator: Si (111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8726 Å / Relative weight: 1 |
Reflection | Resolution: 1.98→78.29 Å / Num. obs: 46107 / % possible obs: 98.1 % / Redundancy: 4 % / Biso Wilson estimate: 34.12 Å2 / CC1/2: 0.994 / Rmerge(I) obs: 0.125 / Net I/σ(I): 7.9 |
Reflection shell | Resolution: 1.98→2.1 Å / Redundancy: 4 % / Rmerge(I) obs: 0.665 / Mean I/σ(I) obs: 1.9 / % possible all: 92.5 |
-Phasing
Phasing | Method: ![]() | ||||||
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Phasing MR | R rigid body: 0.585
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3KS3 Resolution: 1.98→78.29 Å / Cor.coef. Fo:Fc: 0.957 / Cor.coef. Fo:Fc free: 0.941 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.183 / ESU R Free: 0.157 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED
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Solvent computation | Ion probe radii: 0.7 Å / Shrinkage radii: 0.7 Å / VDW probe radii: 1 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 102.8 Å2 / Biso mean: 43.816 Å2 / Biso min: 18.65 Å2
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Refinement step | Cycle: final / Resolution: 1.98→78.29 Å
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Refine LS restraints |
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Refine LS restraints NCS | Ens-ID: 1 / Number: 31998 / Refine-ID: X-RAY DIFFRACTION / Type: interatomic distance / Rms dev position: 0.08 Å / Weight position: 0.05
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LS refinement shell | Resolution: 1.981→2.032 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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