The distance between Residue A GLU 677 and Residue A PHE 681 is 20.95 Angstrom despite the fact ...The distance between Residue A GLU 677 and Residue A PHE 681 is 20.95 Angstrom despite the fact that there are only 3 residues (not enough sequence) to cover the gap region. One possibility for that is some domain swapping for the last two helices (note that E677 is closer to the symmetry related F681). Another possibility is that the protein is clipped. There is a bit of a doublet in the SDS PAGE of this protein.
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.52 Å3/Da / 溶媒含有率: 51.16 %
結晶化
温度: 289 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 8.5 詳細: 20 mg/mL protein against Morpheus D10 with 10% PEG 8000, 20% EG, 0.02 M each alcohol, 0.1 M bicine/Trisma base pH 8.5, crystal tracking ID 263964d10, unique puck ID pyl5-5