Mass: 70331.031 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sulfolobus tokodaii str. 7 (archaea) / Strain: 7 / Gene: STK_23000 / Plasmid: pET17b / Production host: Escherichia coli (E. coli) / Strain (production host): C43 (DE3) References: UniProt: Q96Y66, Oxidoreductases; Acting on the aldehyde or oxo group of donors; With an iron-sulfur protein as acceptor
#2: Protein
2-oxoacid--ferredoxinoxidoreductasebetasubunit
Mass: 33652.621 Da / Num. of mol.: 2 / Mutation: K5T Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sulfolobus tokodaii str. 7 (archaea) / Strain: 7 / Gene: STK_22980 / Plasmid: pET17b / Production host: Escherichia coli (E. coli) / Strain (production host): C43 (DE3) References: UniProt: Q96Y68, Oxidoreductases; Acting on the aldehyde or oxo group of donors; With an iron-sulfur protein as acceptor
Resolution: 2.5→36.47 Å / Cor.coef. Fo:Fc: 0.943 / Cor.coef. Fo:Fc free: 0.909 / SU B: 11.974 / SU ML: 0.269 / Cross valid method: THROUGHOUT / ESU R: 0.651 / ESU R Free: 0.328 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.27799
3274
5.1 %
RANDOM
Rwork
0.21121
-
-
-
obs
0.21466
61283
98.92 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å