温度: 277 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 7 詳細: 3 uL protein (10 mg/mL ATAS, 20 mM MES, pH 6.5, 1.9 mM magnesium chloride, 120 mM sodium chloride, 3 mM BME, 1.6 mM sodium pyrophosphate, 1.5 mM inhibitor) + 3 uL precipitant (100 mM Bis- ...詳細: 3 uL protein (10 mg/mL ATAS, 20 mM MES, pH 6.5, 1.9 mM magnesium chloride, 120 mM sodium chloride, 3 mM BME, 1.6 mM sodium pyrophosphate, 1.5 mM inhibitor) + 3 uL precipitant (100 mM Bis-Tris, pH 7.0, 200 mM lithium sulfate, 27% w/v PEG3350) against 500 uL precipitant, VAPOR DIFFUSION, HANGING DROP, temperature 277K
タイプ: DECTRIS PILATUS 6M / 検出器: PIXEL / 日付: 2012年6月29日 詳細: Meridionally-bent fused silica mirror with palladium and uncoated stripes vertically-focusing at 6.6:1 demagnification
放射
モノクロメーター: Double crystal Si(111) with cryogenically-cooled first crystal and sagittally-bent second crystal horizontally focusing at 3.3:1 demagnification プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray