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- EMDB-4369: Unique features of mammalian mitochondrial translation initiation... -

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Basic information

Entry
Database: EMDB / ID: EMD-4369
TitleUnique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 28S ribosomal subunit.
Map data
Sample
  • Complex: mammalian mitochondrial translation initiation complex
    • Complex: Porcine Ribosome
      • Protein or peptide: x 33 types
      • RNA: x 3 types
    • Complex: Translation initiation factor IF-2, mitochondrial
    • Complex: Nucleic acidsNucleic acid
  • Ligand: x 8 types
Function / homology
Function and homology information


Hormone ligand-binding receptors / gonadotropin-releasing hormone receptor binding / gonadotropin hormone-releasing hormone activity / G alpha (q) signalling events / Mitochondrial translation elongation / Mitochondrial translation termination / mitochondrial translational initiation / : / translation factor activity, RNA binding / ribosome disassembly ...Hormone ligand-binding receptors / gonadotropin-releasing hormone receptor binding / gonadotropin hormone-releasing hormone activity / G alpha (q) signalling events / Mitochondrial translation elongation / Mitochondrial translation termination / mitochondrial translational initiation / : / translation factor activity, RNA binding / ribosome disassembly / mitochondrial ribosome assembly / Mitochondrial translation initiation / mitochondrial small ribosomal subunit / regulation of translational initiation / mitochondrial ribosome / mitochondrial translation / positive regulation of proteolysis / ribosomal small subunit binding / organelle membrane / translation initiation factor activity / small ribosomal subunit rRNA binding / ribosomal small subunit assembly / regulation of translation / cell junction / small ribosomal subunit / nuclear membrane / rRNA binding / ribosome / mitochondrial matrix / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / intracellular membrane-bounded organelle / GTPase activity / apoptotic process / GTP binding / nucleolus / mitochondrion / extracellular space / RNA binding / nucleoplasm / cytoplasm
Similarity search - Function
Gonadotropin-releasing hormone / Gonadoliberin / Gonadotropin-releasing hormone / Gonadotropin-releasing hormones signature. / Translation initiation factor IF-2, domain II / Initiation factor 2 signature. / Translation initiation factor IF-2, bacterial-like / Coiled-coil-helix-coiled-coil-helix domain-containing protein 1 / Translation initiation factor IF- 2, domain 3 / Translation-initiation factor 2 ...Gonadotropin-releasing hormone / Gonadoliberin / Gonadotropin-releasing hormone / Gonadotropin-releasing hormones signature. / Translation initiation factor IF-2, domain II / Initiation factor 2 signature. / Translation initiation factor IF-2, bacterial-like / Coiled-coil-helix-coiled-coil-helix domain-containing protein 1 / Translation initiation factor IF- 2, domain 3 / Translation-initiation factor 2 / Translation initiation factor IF- 2 / Translation initiation factor IF-2, domain 3 superfamily / Cysteine alpha-hairpin motif superfamily / 28S ribosomal protein S27, mitochondrial / MRPS27/PTCD2 / Mitochondrial 28S ribosomal protein S27 / Ribosomal protein S22, mitochondrial / Ribosomal protein S23, mitochondrial / Ribosomal protein S23/S25, mitochondrial / Mitochondrial 28S ribosomal protein S22 / Mitochondrial ribosomal protein S23 / Ribosomal protein S29, mitochondrial / Ribosomal protein S28, mitochondrial / 28S ribosomal protein S10, mitochondrial / Mitochondrial ribosomal protein MRP-S35 / 28S ribosomal protein S24, mitochondrial / Mitochondrial 28S ribosomal protein S34 / Pentatricopeptide repeat domain-containing protein 3 / 28S ribosomal protein S17, mitochondrial / 28S ribosomal protein S18b, mitochondrial / : / Mitochondrial ribosome subunit S24 / Mitochondrial 28S ribosomal protein S34 / 40S ribosomal protein rpS2 (S5p), N-terminal / 28S ribosomal protein S25, mitochondrial / Pentatricopeptide repeat domain / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial ribosomal subunit protein / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Pentatricopeptide repeat / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial domain of unknown function (DUF1713) / CHCH / CHCH domain / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Translational (tr)-type GTP-binding domain / Elongation factor Tu GTP binding domain / Translational (tr)-type guanine nucleotide-binding (G) domain profile. / Ribosomal protein S21 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein L19 / Ribosomal protein L19 superfamily / Ribosomal protein L19 / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein S12, bacterial-type / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 / Ribosomal protein S18 / Ribosomal protein S18 superfamily / Ribosomal protein S2 signature 1. / Ribosomal protein S2, conserved site / Ribosomal protein S2 / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S2 / Ribosomal protein S14 / Ribosomal protein S14p/S29e / Ribosomal protein S5, N-terminal, conserved site / Ribosomal protein S5 signature. / Ribosomal protein S5 / Ribosomal protein S5, N-terminal / Ribosomal S11, conserved site / Ribosomal protein S10p/S20e / S5 double stranded RNA-binding domain profile. / Ribosomal protein S5, C-terminal / Ribosomal protein S9, conserved site / Ribosomal protein S5, N-terminal domain / Ribosomal protein S10 domain / Ribosomal protein S10 domain superfamily / Ribosomal protein S5, C-terminal domain / Ribosomal protein S11 / Ribosomal protein S5/S7 / Ribosomal protein S7 domain / Ribosomal protein S7 domain superfamily / Ribosomal protein S10p/S20e / Ribosomal protein S9 / Ribosomal protein S11 / Ribosomal protein S17/S11
Similarity search - Domain/homology
Small ribosomal subunit protein uS12m / 28S ribosomal protein S14, mitochondrial / 28S ribosomal protein S18c, mitochondrial isoform 1 / Small ribosomal subunit protein mS25 / 28S ribosomal protein S28, mitochondrial / Small ribosomal subunit protein uS7m / Mitochondrial ribosomal protein S11 / 28S ribosomal protein S21, mitochondrial / Mitochondrial ribosomal protein S23 / 28S ribosomal protein S24, mitochondrial ...Small ribosomal subunit protein uS12m / 28S ribosomal protein S14, mitochondrial / 28S ribosomal protein S18c, mitochondrial isoform 1 / Small ribosomal subunit protein mS25 / 28S ribosomal protein S28, mitochondrial / Small ribosomal subunit protein uS7m / Mitochondrial ribosomal protein S11 / 28S ribosomal protein S21, mitochondrial / Mitochondrial ribosomal protein S23 / 28S ribosomal protein S24, mitochondrial / Mitochondrial ribosomal protein S22 / Progonadoliberin / Small ribosomal subunit protein mS38 / Small ribosomal subunit protein uS15m / Uncharacterized protein / Mitochondrial ribosomal protein S17 / Small ribosomal subunit protein uS10m / 28S ribosomal protein S2, mitochondrial / Small ribosomal subunit protein mS33 / 28S ribosomal protein S35, mitochondrial isoform 1 / Coiled-coil-helix-coiled-coil-helix domain containing 1 / Small ribosomal subunit protein uS5m / Mitochondrial ribosomal protein S6 / Mitochondrial ribosomal protein S27 / Large ribosomal subunit protein bL19m / Small ribosomal subunit protein mS29 / 28S ribosomal protein S9, mitochondrial / Small ribosomal subunit protein mS39 / Translation initiation factor IF-2, mitochondrial / Small ribosomal subunit protein mS40
Similarity search - Component
Biological speciesSus scrofa (pig) / Homo sapiens (human) / Pig (pig)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.1 Å
AuthorsKummer E / Leibundgut M / Boehringer D / Ban N
Funding support Switzerland, 3 items
OrganizationGrant numberCountry
European Molecular Biology OrganizationALTF 1196-2014 Switzerland
Swiss National Science Foundation138262 Switzerland
Swiss National Science Foundation310030B_163478 Switzerland
CitationJournal: Nature / Year: 2018
Title: Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM.
Authors: Eva Kummer / Marc Leibundgut / Oliver Rackham / Richard G Lee / Daniel Boehringer / Aleksandra Filipovska / Nenad Ban /
Abstract: Mitochondria maintain their own specialized protein synthesis machinery, which in mammals is used exclusively for the synthesis of the membrane proteins responsible for oxidative phosphorylation. The ...Mitochondria maintain their own specialized protein synthesis machinery, which in mammals is used exclusively for the synthesis of the membrane proteins responsible for oxidative phosphorylation. The initiation of protein synthesis in mitochondria differs substantially from bacterial or cytosolic translation systems. Mitochondrial translation initiation lacks initiation factor 1, which is essential in all other translation systems from bacteria to mammals. Furthermore, only one type of methionyl transfer RNA (tRNA) is used for both initiation and elongation, necessitating that the initiation factor specifically recognizes the formylated version of tRNA (fMet-tRNA). Lastly, most mitochondrial mRNAs do not possess 5' leader sequences to promote mRNA binding to the ribosome. There is currently little mechanistic insight into mammalian mitochondrial translation initiation, and it is not clear how mRNA engagement, initiator-tRNA recruitment and start-codon selection occur. Here we determine the cryo-EM structure of the complete translation initiation complex from mammalian mitochondria at 3.2 Å. We describe the function of an additional domain insertion that is present in the mammalian mitochondrial initiation factor 2 (mtIF2). By closing the decoding centre, this insertion stabilizes the binding of leaderless mRNAs and induces conformational changes in the rRNA nucleotides involved in decoding. We identify unique features of mtIF2 that are required for specific recognition of fMet-tRNA and regulation of its GTPase activity. Finally, we observe that the ribosomal tunnel in the initiating ribosome is blocked by insertion of the N-terminal portion of mitochondrial protein mL45, which becomes exposed as the ribosome switches to elongation mode and may have an additional role in targeting of mitochondrial ribosomes to the protein-conducting pore in the inner mitochondrial membrane.
History
DepositionApr 13, 2018-
Header (metadata) releaseMay 9, 2018-
Map releaseAug 8, 2018-
UpdateDec 18, 2019-
Current statusDec 18, 2019Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.085
  • Imaged by UCSF Chimera
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  • Surface view colored by height
  • Surface level: 0.085
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6gaz
  • Surface level: 0.085
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_4369.png

Downloads & links

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Map

FileDownload / File: emd_4369.map.gz / Format: CCP4 / Size: 84.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 1.39 Å
Density
Contour LevelBy AUTHOR: 0.085 / Movie #1: 0.085
Minimum - Maximum-0.24249981 - 0.60988814
Average (Standard dev.)0.0011413419 (±0.017309353)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions281281281
Spacing281281281
CellA=B=C: 390.59 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.391.391.39
M x/y/z281281281
origin x/y/z0.0000.0000.000
length x/y/z390.590390.590390.590
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS281281281
D min/max/mean-0.2420.6100.001

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Supplemental data

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Mask #1

Fileemd_4369_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_4369_half_map_1.map
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_4369_half_map_2.map
Projections & Slices
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Sample components

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Entire : mammalian mitochondrial translation initiation complex

EntireName: mammalian mitochondrial translation initiation complex
Components
  • Complex: mammalian mitochondrial translation initiation complex
    • Complex: Porcine Ribosome
      • Protein or peptide: Translation initiation factor IF-2, mitochondrial
      • Protein or peptide: Mitochondrial ribosomal protein L19
      • RNA: 12S ribosomal RNA, mitochondrial
      • Protein or peptide: Mitochondrial ribosomal protein S2
      • Protein or peptide: Mitochondrial ribosomal protein S24
      • Protein or peptide: Mitochondrial ribosomal protein S5
      • Protein or peptide: Mitochondrial ribosomal protein S6
      • Protein or peptide: Mitochondrial ribosomal protein S7
      • Protein or peptide: Mitochondrial ribosomal protein S9
      • Protein or peptide: Mitochondrial ribosomal protein S10
      • Protein or peptide: Mitochondrial ribosomal protein S11
      • Protein or peptide: Mitochondrial ribosomal protein S12
      • Protein or peptide: Mitochondrial ribosomal protein S14
      • Protein or peptide: Mitochondrial ribosomal protein S15
      • Protein or peptide: bS16m, MRPS16
      • Protein or peptide: Mitochondrial ribosomal protein S17
      • Protein or peptide: Mitochondrial ribosomal protein S18C
      • Protein or peptide: Mitochondrial ribosomal protein S21
      • RNA: P-site fMet-tRNAMet, mitochondrial
      • RNA: MT-CO3 mRNA, mitochondrial
      • Protein or peptide: unassigned secondary structure elements
      • Protein or peptide: Mitochondrial ribosomal protein S22
      • Protein or peptide: Mitochondrial ribosomal protein S23
      • Protein or peptide: Mitochondrial ribosomal protein S25
      • Protein or peptide: Mitochondrial ribosomal protein S26
      • Protein or peptide: Mitochondrial ribosomal protein S27
      • Protein or peptide: Mitoribosomal protein ms28, mrps28
      • Protein or peptide: Death associated protein 3
      • Protein or peptide: mS31, MRPS31
      • Protein or peptide: Mitochondrial ribosomal protein S33
      • Protein or peptide: Mitochondrial ribosomal protein S34
      • Protein or peptide: Mitochondrial ribosomal protein S35
      • Protein or peptide: Mitochondrial ribosomal protein S37
      • Protein or peptide: Aurora kinase A interacting protein 1
      • Protein or peptide: Mitochondrial ribosomal protein S39
      • Protein or peptide: 28S ribosomal protein S18b, mitochondrialRibosome
    • Complex: Translation initiation factor IF-2, mitochondrial
    • Complex: Nucleic acidsNucleic acid
  • Ligand: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE
  • Ligand: MAGNESIUM ION
  • Ligand: SODIUM IONSodium
  • Ligand: SPERMINE
  • Ligand: ZINC ION
  • Ligand: N-FORMYLMETHIONINE
  • Ligand: GUANOSINE-5'-TRIPHOSPHATEGuanosine triphosphate
  • Ligand: water

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Supramolecule #1: mammalian mitochondrial translation initiation complex

SupramoleculeName: mammalian mitochondrial translation initiation complex
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#36
Molecular weightTheoretical: 2.85 MDa

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Supramolecule #2: Porcine Ribosome

SupramoleculeName: Porcine Ribosome / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#36
Source (natural)Organism: Sus scrofa (pig)

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Supramolecule #3: Translation initiation factor IF-2, mitochondrial

SupramoleculeName: Translation initiation factor IF-2, mitochondrial / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #1
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Supramolecule #4: Nucleic acids

SupramoleculeName: Nucleic acids / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #19-#20
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: synthetic construct (others)

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Macromolecule #1: Translation initiation factor IF-2, mitochondrial

MacromoleculeName: Translation initiation factor IF-2, mitochondrial / type: protein_or_peptide / ID: 1
Details: Unassigned residues have been built as poly-alanine.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 72.811219 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: SGGSGSGWKS QLSSTKSKKV VEVWIGMTIE ELARAMEKNT DYVYEALLNT DIDIDSLEAD SHLDEVWIKE VITKAGMKLK WSKLKQDKV RKNKDAVRRP QADPALLTPR SPVVTIMGHV DHGKTTLLDK FRKTQVAAVE TGGITQHIGA FLVSLPSGEK I TFLDTPGH ...String:
SGGSGSGWKS QLSSTKSKKV VEVWIGMTIE ELARAMEKNT DYVYEALLNT DIDIDSLEAD SHLDEVWIKE VITKAGMKLK WSKLKQDKV RKNKDAVRRP QADPALLTPR SPVVTIMGHV DHGKTTLLDK FRKTQVAAVE TGGITQHIGA FLVSLPSGEK I TFLDTPGH AAFSAMRARG AQVTDIVVLV VAADDGVMKQ TVESIQHAKD AQVPIILAVN KCDKAEADPE KVKKELLAYD VV CEDYGGD VQAVPVSALT GDNLMALAEA TVALAEMLEL KADPNGPVEG TVIESFTDKG RGLVTTAIIQ RGTLRKGSVL VAG KCWAKV RLMFDENGKT IDEAYPSMPV GITGWRDLPS AGEEILEVES EPRAREVVDW RKYEQEQEKG QEDLKIIEEK RKEH KEAHQ KAREKYGHLL WKKRSILRFL ERKEQIPLKP KEKRERDSNV LSVIIKGDVD GSVEAILNII DTYDASHECE LELVH FGVG DVSANDVNLA ETFDGVIYGF NVNAGNVIQQ SAAKKGVKIK LHKIIYRLVE DLQEELSSRL PCAVEEHPVG EASILA TFS VTEGKKKVPV AGCRVQKGQL EKQKKFKLTR NGHVIWKGSL TSLKHHKDDI SIVKTGMDCG LSLDEDNMEF QVGDRIV CY EEKQIQAKTS WDPGF

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Macromolecule #2: Mitochondrial ribosomal protein L19

MacromoleculeName: Mitochondrial ribosomal protein L19 / type: protein_or_peptide / ID: 2
Details: Unassigned residues have been built as poly-alanine.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 33.441539 KDa
SequenceString: MAASITRGSW AAVGLGQSFP PTRALLPAPA SVICRTLAGS GRQQVTGSSE PGAFQPPPKP VIVDKRRPQR PETRFLSPEF IPPRGRTNP LKFQIERKDM LERRKILHIP EFYVGSILRV TTADPYASAK TSQFLGICIQ RSGSGLGATF ILRNTIEGQG V EICFELYN ...String:
MAASITRGSW AAVGLGQSFP PTRALLPAPA SVICRTLAGS GRQQVTGSSE PGAFQPPPKP VIVDKRRPQR PETRFLSPEF IPPRGRTNP LKFQIERKDM LERRKILHIP EFYVGSILRV TTADPYASAK TSQFLGICIQ RSGSGLGATF ILRNTIEGQG V EICFELYN PRIQEIQVVK LEKRLDNSLL YLRDALPEYS TFDVNMKPIT QEFNQEVPVN QLKVKMKPKP WSKRWERPKF NI KGIRFDL CLTEEQMREA QKWSQPWLEF DMMREYDTSK IEAAIWDEIE ASKNS

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Macromolecule #4: Mitochondrial ribosomal protein S2

MacromoleculeName: Mitochondrial ribosomal protein S2 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 31.731604 KDa
SequenceString: MASGPLLPRL LGAGAPRRPL RLHLVPTATP GPAGPTGRTL GSNAAPAARE PELGNDLRDR ILSEPLKHAD FFNLKELFSV RSLFDARVH LGHKAGCRHR FMEPYLFGSR LGQDIIDLEQ TAAHLQLALN FTAHVAYREG IILFVSRHRQ FAHLIETTAR D CGEYAHTR ...String:
MASGPLLPRL LGAGAPRRPL RLHLVPTATP GPAGPTGRTL GSNAAPAARE PELGNDLRDR ILSEPLKHAD FFNLKELFSV RSLFDARVH LGHKAGCRHR FMEPYLFGSR LGQDIIDLEQ TAAHLQLALN FTAHVAYREG IILFVSRHRQ FAHLIETTAR D CGEYAHTR YFKGGLLTNA PLLLGPGVRL PDLIIFLHTL NNVFEPHVAV RDAAKMNIPT VGIVDTNCNP ALITYPVPGN DD SPPAVRL FCRLFQVAIS RAKEKRRQVE ALYRLQGPAG AERGPAAPPP PE

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Macromolecule #5: Mitochondrial ribosomal protein S24

MacromoleculeName: Mitochondrial ribosomal protein S24 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 18.935172 KDa
SequenceString:
MVASVCGGGL RPLVLSWSRE LPCAWRALHI SAVCAKNRAA RVRVSKGDKP VTYEEAHAPH YIAHRKGWLS LHTGNLDGED HAAERTVED VFLRKFMLGT FPGCLADQLV LKRRANQLEI CALVLRQLPP HKFYFLVGYS ETLLSHFYKC PVHLHLQTVP S KVVYKYI

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Macromolecule #6: Mitochondrial ribosomal protein S5

MacromoleculeName: Mitochondrial ribosomal protein S5 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 48.213074 KDa
SequenceString: MAAAVRAAGF FPGLFGASAG NLWSRQLYLN TFPAASILTS KTFPSNGPLS PPGTRDNHHF TSLACALQTQ CSISSPSNWM GQQYRSYSF FTKLTADELW KGALAESGAG ARKGRGKRTK KKRRKDLNRG QIIGEGRHGF LWPGLNIPLM RNGAVQTIAQ R SKEDQEKV ...String:
MAAAVRAAGF FPGLFGASAG NLWSRQLYLN TFPAASILTS KTFPSNGPLS PPGTRDNHHF TSLACALQTQ CSISSPSNWM GQQYRSYSF FTKLTADELW KGALAESGAG ARKGRGKRTK KKRRKDLNRG QIIGEGRHGF LWPGLNIPLM RNGAVQTIAQ R SKEDQEKV EADMVQQREE WDRRRKMKVK RERGWSGNTW GGVSLGPPDP GPNGETYDDF DTRILEVRNV FNMTAKEGRK RS VRVLVAV GNGKGAAGFA IGKATERADA FRKAKNRAVH YLHYIERYED HTIYHDISLK FKRTHIKMKK QPRGYGLHCH RAI MTICRL IGIKDLYAKV SGSVNMLNLT RGLFLGLSRQ ETHQQLADKK SLHVVEFREE CGPLPIVVAS PQGALRKDPE PEDE VPDIT LDWEDVKAAQ GMKRSVWSGL KRAAT

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Macromolecule #7: Mitochondrial ribosomal protein S6

MacromoleculeName: Mitochondrial ribosomal protein S6 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 14.283703 KDa
SequenceString:
MPRYELALIL KAMQRPETAA ALKRTLEALM DRGAVVRNLE NLGERMLPYK ISAHNQRHSR GGYFLVDFYA PATTVESMME HLSRDIDVI RPNIVKHPLT QEVKECEGIV PVPLEEKLYS TKKRK

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Macromolecule #8: Mitochondrial ribosomal protein S7

MacromoleculeName: Mitochondrial ribosomal protein S7 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 28.150918 KDa
SequenceString: MAAPAVKAAR GWSCSALGVR IAVLRFPGLT QVRWSRYGPE YKDPQIDKEY YRKPLAEQTE EEKYERDFKK TQLIKAAPAT KTSSVFEDP VISKFTNMMM KGGNKVLARS LMTQTLEAVK RKQFAKYHAA SAEEQATIER NPYTIFHQAL KNCEPVIGLV P ILKGGHFY ...String:
MAAPAVKAAR GWSCSALGVR IAVLRFPGLT QVRWSRYGPE YKDPQIDKEY YRKPLAEQTE EEKYERDFKK TQLIKAAPAT KTSSVFEDP VISKFTNMMM KGGNKVLARS LMTQTLEAVK RKQFAKYHAA SAEEQATIER NPYTIFHQAL KNCEPVIGLV P ILKGGHFY QVPVPLADRR RRFLAMKWMI AECREKKHRR VLMPEKLSQE LLEAFHNQGP VIKRKHDMHK MAEANRALAH YR WW

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Macromolecule #9: Mitochondrial ribosomal protein S9

MacromoleculeName: Mitochondrial ribosomal protein S9 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 45.60457 KDa
SequenceString: MAAPCVFCGA AVSYRLFLGC RVSFARGQVL WKAAAPELQA GARCQILRLR HTAVVTTKKN VAASRRETYT VDFIKKQIEE FNIGKRHLA NMMGEDPETF TQEDIDRAIA YLFPSGLFEK RARPIMKHPE EIFPKQRAIQ WGEDGRPFHF LFYTGKQSYY S LMHDTYGK ...String:
MAAPCVFCGA AVSYRLFLGC RVSFARGQVL WKAAAPELQA GARCQILRLR HTAVVTTKKN VAASRRETYT VDFIKKQIEE FNIGKRHLA NMMGEDPETF TQEDIDRAIA YLFPSGLFEK RARPIMKHPE EIFPKQRAIQ WGEDGRPFHF LFYTGKQSYY S LMHDTYGK LLDVEKHHNQ LRAKDLLAEK TKILKDPIGS RWLIKEELEE MLVEKLSDQD YAQFIRLLER LSALPCGATE ED FVNRFRR SIPIQSKKQL IEPLQYDEQG MAFSRGEGKR KTAKAEVVVY GQGSGRIDVN GVDYLLYFPV TQDREQLMFP LHF LDRLGK HDMTCAVSGG GRSAQAGAVR LAMARALCSF VTEDEVEWMR QAGLLTADPR VRERKKPGQE GARRKFTWKK R

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Macromolecule #10: Mitochondrial ribosomal protein S10

MacromoleculeName: Mitochondrial ribosomal protein S10 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 23.192758 KDa
SequenceString: MAARTAFGAL ARRLWQGSRN FSVSSSRSST AKNGGFLLST TMKWVQFSNL HVDVPKDMTK PEITISDEPD TLYKRLSVLV KGHDKAVLD SYEYFAVLAA KELGISIKVH EPPRKIERFT LLKSVHIFKK HRVQYEMRTL YRCLELEHLT GSTADVYLEY I QRNLPEGV ...String:
MAARTAFGAL ARRLWQGSRN FSVSSSRSST AKNGGFLLST TMKWVQFSNL HVDVPKDMTK PEITISDEPD TLYKRLSVLV KGHDKAVLD SYEYFAVLAA KELGISIKVH EPPRKIERFT LLKSVHIFKK HRVQYEMRTL YRCLELEHLT GSTADVYLEY I QRNLPEGV AMEVTKTKLE QLPEHIRKPI WETMPEEKEE SKS

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Macromolecule #11: Mitochondrial ribosomal protein S11

MacromoleculeName: Mitochondrial ribosomal protein S11 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 20.841922 KDa
SequenceString: MQVVRNAGSR LLQSWAWPPK TRIVAGLPAS TIHRSAPHLQ DAAAKLDIED KEEARTPSHS SFSIYPPIPG QENSLRWAGK KFEEIPIAH IKASYNNTQI QVVSAAHQPL AHASCGTEGF RNAKKGTGIA AQTAGIAAAA KATGKGVTHV RVVVKGLGPG R LSAIKGLT ...String:
MQVVRNAGSR LLQSWAWPPK TRIVAGLPAS TIHRSAPHLQ DAAAKLDIED KEEARTPSHS SFSIYPPIPG QENSLRWAGK KFEEIPIAH IKASYNNTQI QVVSAAHQPL AHASCGTEGF RNAKKGTGIA AQTAGIAAAA KATGKGVTHV RVVVKGLGPG R LSAIKGLT MGGLEVISIT DNTPIPHNGC RPRKARRL

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Macromolecule #12: Mitochondrial ribosomal protein S12

MacromoleculeName: Mitochondrial ribosomal protein S12 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 15.257107 KDa
SequenceString:
MSWSGLLRGL GTSLNCGLAL VPRPWATRPM ATLNQMHRLG PPKHPPGKMG PTAGRPQLKG VVLRTFIRKP KKPNSANRKC CRVRLSTGR EAVCFIPGEG HSLQEHHVVL VQGGRTQDLP GVKLTVVRGK YDCGHVQKKK

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Macromolecule #13: Mitochondrial ribosomal protein S14

MacromoleculeName: Mitochondrial ribosomal protein S14 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 15.120739 KDa
SequenceString:
MAASMLGFLL RTVRQMVPSS ASGQVRSYYV DWKMLRDVKR RKMAYEYADE RLRINSLRKN TILPKDLQEV ADEEIASLPR DSCPVRIRN RCVMTSRPRG VKRRWRLSRI VFRHLADHGQ LSGVQRAMW

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Macromolecule #14: Mitochondrial ribosomal protein S15

MacromoleculeName: Mitochondrial ribosomal protein S15 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 27.411428 KDa
SequenceString: MLRAAWRALS SIRTQAVTQA PVLGLPGGGC ARFPSVQRAL PSRPAGLILR AARGYAAQKP VQPNQDDPPP SMLLLDYQNV PGIHKVDDV VKRLLSLEMA NQKEKLKIKK MQLMNKVLEN PEDTSSLEAR IVALTVKIRN YEEHMQKHRK DKAHKRFLLM S IDQRKKML ...String:
MLRAAWRALS SIRTQAVTQA PVLGLPGGGC ARFPSVQRAL PSRPAGLILR AARGYAAQKP VQPNQDDPPP SMLLLDYQNV PGIHKVDDV VKRLLSLEMA NQKEKLKIKK MQLMNKVLEN PEDTSSLEAR IVALTVKIRN YEEHMQKHRK DKAHKRFLLM S IDQRKKML KNLRETNYAV FEKICKELGI EYTFPPPYHR KAHRRWVTKK ALCTQVFREV QKLKKQKRAL RAAAVAAHKQ G

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Macromolecule #15: bS16m, MRPS16

MacromoleculeName: bS16m, MRPS16 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: liver
Molecular weightTheoretical: 15.182668 KDa
SequenceString:
MVQLTTVLCR AYRGGHLTIR LALGGCTNRP FYRIVAAHSK CPRDGRFVEQ LGSYDPLPNS HGEKLVALNF DRIRHWIGCG AHLSKPVEK LLGLSGFYPL HPMMITNAER LRRKRAREVL LASQKTDTEA TETKAS

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Macromolecule #16: Mitochondrial ribosomal protein S17

MacromoleculeName: Mitochondrial ribosomal protein S17 / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 14.452014 KDa
SequenceString:
MSIARSSVHA KWVVGKVIGT AMQKTAKVRV TRLVLDPYLL KYFNKRKTYF AHDALQQCTV GDIVLLKALP VPRTKHVKHE LAEIIFKVG QVIDPVTGKP CAGTTYLESP ISPESTRLTK NLEALQLSST Q

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Macromolecule #17: Mitochondrial ribosomal protein S18C

MacromoleculeName: Mitochondrial ribosomal protein S18C / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 16.211086 KDa
SequenceString:
MAAVVSLCSR LGRRKSTDFV NAAVCLTDPG THAVLWRSCS QYKQVASNED LPVPMENPYK EPLKKCILCE KHVDYKNVQL LSQFISPFT GCIYGRHITG LCGKKQKEIT KAIKRAQILG FMPVTYKDPA YLKDPKVCNI KYRE

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Macromolecule #18: Mitochondrial ribosomal protein S21

MacromoleculeName: Mitochondrial ribosomal protein S21 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 10.682506 KDa
SequenceString:
MANHLKFIAR TVMVQEGNVE GAYRTLNRIL TMDGLIEDIK RRRYYEKPCR RRQRESYETC RRIYNMEMAR KINFLMRKNR ADPWQGC

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Macromolecule #21: unassigned secondary structure elements

MacromoleculeName: unassigned secondary structure elements / type: protein_or_peptide / ID: 21
Details: Unassigned secondary structure elements have been built as poly-alanine.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Sus scrofa (pig) / Organ: liver
Molecular weightTheoretical: 1.297416 KDa
SequenceString:
AAAAAAAAAA AAAAAAAA

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Macromolecule #22: Mitochondrial ribosomal protein S22

MacromoleculeName: Mitochondrial ribosomal protein S22 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 43.234645 KDa
SequenceString: MPHEPRETRN LSLPLPALVA RIIMATLKAS ALLRSLQTNS CGTGRVCFPV RARPRPRALL QPLPGASGTG TLCRGLGSES ESGNSEIRK PTFMDEEVQN ILIKMTGLDL QKIFKPALQE LKPPTYKLMT QAQLEEATKQ AVEAAKVRLK MPPVLEERAP I NDVLAEDK ...String:
MPHEPRETRN LSLPLPALVA RIIMATLKAS ALLRSLQTNS CGTGRVCFPV RARPRPRALL QPLPGASGTG TLCRGLGSES ESGNSEIRK PTFMDEEVQN ILIKMTGLDL QKIFKPALQE LKPPTYKLMT QAQLEEATKQ AVEAAKVRLK MPPVLEERAP I NDVLAEDK ILEGTETAKY VFTDISYSIP HRERFIVVRE PSGTLRKASW EERDRMIQVY FPREGRRILT PVIFKEENLQ TM YSQDQHV DVLNLCVAQF EPDSAEYIKI HHHTYEDIDK CGKYDLLRST RHFGGMAWYF VNKKKIDGLL IDQIQRDLVS DAT SLVHLY HILHPDGQSA QEAKKQGAEG LHLIKVFAKT EAQKGAYIEL TLQAYQEAFI THSAAS

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Macromolecule #23: Mitochondrial ribosomal protein S23

MacromoleculeName: Mitochondrial ribosomal protein S23 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 21.666674 KDa
SequenceString:
MAGSRLETVG SIFSRTRDLI RAGVLKEKPL WLDIYNAFPP LREPVFRRPR LRYGKAKAAV QDIFYHEDRI RAKFYSAYGS GPKAFDLFN PNFKSTCQRF VEKYIELQRL GETDEEKLFV EAGKALLAEG VTLRRVGEAR TQQEGSPISR KSEPTGIKPQ T AVEENQPW KEVPQDQPLE APEKESKGLP PP

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Macromolecule #24: Mitochondrial ribosomal protein S25

MacromoleculeName: Mitochondrial ribosomal protein S25 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 19.954186 KDa
SequenceString:
MPMKGRFPIR RTLQYLGQGD VVFKDSVKVM TVNYNTHGEL GEGARKFVFF NIPQIQYKNP WVQITMFKNM TPSPFLRFYL DSGEQVLVD VESKSNKEIV EHIRKILGKN EETLEKEEQE KKQLSHPAHF GPRKYCLREC MCEVEGQVPC PGLVPLPKEM T GKYKAALK ATAQD

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Macromolecule #25: Mitochondrial ribosomal protein S26

MacromoleculeName: Mitochondrial ribosomal protein S26 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 23.6379 KDa
SequenceString: MLRVLGGLGA RPAGRLPAPL LLPTRGRKTR HDPPAKSKAG RVATPPAVDP TEFFVLTERY RQYRQTVRAL RLEFMSEVRK KLHEARAGV QAERKAQEDA AEHRELMAWN QAENQRLHEL RLARLRQEAL EQERRQAEEA VLQAREAQAW AQLKEQEVLQ L QEEAKTFI ...String:
MLRVLGGLGA RPAGRLPAPL LLPTRGRKTR HDPPAKSKAG RVATPPAVDP TEFFVLTERY RQYRQTVRAL RLEFMSEVRK KLHEARAGV QAERKAQEDA AEHRELMAWN QAENQRLHEL RLARLRQEAL EQERRQAEEA VLQAREAQAW AQLKEQEVLQ L QEEAKTFI TRENLEARVE EALDSPKSYN WAITREGLVV RPQQKGS

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Macromolecule #26: Mitochondrial ribosomal protein S27

MacromoleculeName: Mitochondrial ribosomal protein S27 / type: protein_or_peptide / ID: 26
Details: Unassigned residues have been built as poly-alanine.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 45.244129 KDa
SequenceString: RRCLLSAAYV DSHKWEARDK EDCHLADLAS LMDKTYERKL PVSSLTISRF VDNIASREDI DHAEYYLYKF RHSPNCWYLR DWTIHTWIR QCLKYGAQDK ALYTLKNKVQ YGIFPDNYTF NLLMDYFIKK ENYKDALSVV FEIMMQEAFE VPSTQLLSLY V LYHCLAEK ...String:
RRCLLSAAYV DSHKWEARDK EDCHLADLAS LMDKTYERKL PVSSLTISRF VDNIASREDI DHAEYYLYKF RHSPNCWYLR DWTIHTWIR QCLKYGAQDK ALYTLKNKVQ YGIFPDNYTF NLLMDYFIKK ENYKDALSVV FEIMMQEAFE VPSTQLLSLY V LYHCLAEK TDLSWEEERN FGASLLLPGL KQKNSVGLSS QLYGYALLGK VELQQGLRAV YCNMPLLWQP GYLHRALQVM EK VASSPED GQLCGEALDV LDRVLKAVTA PAQGTSEEQP QEGEESQRSE ELVEQLDVEE TEQSKLPQYL ERFEALRSKL QAL GKVESE SLLTLSTQLA KAQLPTCEAE DIAVYEQRLQ QWHGERVRLI QREQEQRERA RQDYEARRAA KASA

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Macromolecule #27: Mitoribosomal protein ms28, mrps28

MacromoleculeName: Mitoribosomal protein ms28, mrps28 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 21.014912 KDa
SequenceString:
MAALCRTRAV TAKSHFLRVF FFSRPCRGTG TESGSGSESS ESTEPKQRPG GFASALERHS ELQQKAEFGR MRGSPKNVES FASMLRHSP LTQMGPAKNK LVIGQIFHIV EDDLYIDFGG KFHCVCKRPE VDGEKYQKGT RVRLRLLDLE LTSRFLGATT D TTILEADA VLLGLQESKD SKSKEERHEK

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Macromolecule #28: Death associated protein 3

MacromoleculeName: Death associated protein 3 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 45.582414 KDa
SequenceString: MLKGVTRLIS RVHKLDPGHV LYMGTQAPQS LAAHLDNQVP VKSPRAISRT SEDDPAKHRE QHEGQHYNIS LQELKTVFPH GLPPRFAMQ VKTFNEACLM VRKPALELLH YLKNTNFAHP AVRYVLYGEK GTGKTLSLCH ILHFCAKQNW LILHIPDAHI W VKNCRDLL ...String:
MLKGVTRLIS RVHKLDPGHV LYMGTQAPQS LAAHLDNQVP VKSPRAISRT SEDDPAKHRE QHEGQHYNIS LQELKTVFPH GLPPRFAMQ VKTFNEACLM VRKPALELLH YLKNTNFAHP AVRYVLYGEK GTGKTLSLCH ILHFCAKQNW LILHIPDAHI W VKNCRDLL QSNYNKQRFD QPLEASTWLK NFKTANEHFL SQIKVQEKYV WNKRESTEKG RPLGEVVEQG IMRVRNATDA VG IVLKELK RQSSLGIFHL LVAVDGVNAL WGRTTLKRED KSPIAPEELA LIHNLRKMVK NDWQGGAIVL TVSQTGSLFK PRN AYLPQE LLGKEGFDAL DPFIPILVSN YNPKEFESCI QYYLENNWLQ HEKAHTEEGK KELLFLSNRN PGQLERLCAY L

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Macromolecule #29: mS31, MRPS31

MacromoleculeName: mS31, MRPS31 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 44.091996 KDa
SequenceString: MLPRVSAVIP LRPLSRLPLC SGGPEASAAA ILLLDSLRGT VRTENNIQRY FGTNNLVYSK KDEQSGPTCE ISKETESKGS VKENSKKDL LNIIKGMKVE LSTVNVQTTK PSNKRQLKNL ETAIGRIKKA PEDTPQKRSE SLSSELVAAV SAVADSLPFD R RMTKSELL ...String:
MLPRVSAVIP LRPLSRLPLC SGGPEASAAA ILLLDSLRGT VRTENNIQRY FGTNNLVYSK KDEQSGPTCE ISKETESKGS VKENSKKDL LNIIKGMKVE LSTVNVQTTK PSNKRQLKNL ETAIGRIKKA PEDTPQKRSE SLSSELVAAV SAVADSLPFD R RMTKSELL RQLQKHEEDS R(UNK)QKDGEKPK ISFSNIISDM KVARSATARV STRPVHQIQF DEGTDDFADQ EKTLNLKK S FRKNIFKGRR LNIFEPQAVT EEAPETEAVP SLWDIEFAKQ LAAVTERPFQ NGFEEMIQWT KEGKLWEFPI NNEAGFDDD GSEFHEHIFL DKYLQDFPKQ GPIRHFMELV TCGLSKNPYL SVKQKVEHIE WFRNYFNEKR VILKESGIQL N

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Macromolecule #30: Mitochondrial ribosomal protein S33

MacromoleculeName: Mitochondrial ribosomal protein S33 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 12.54465 KDa
SequenceString:
MSSLSEYAVR MSRLSARLFG EVARPTDSKS MKVVKLFSEQ PLAKRKETYD WYPNHNTYFA LMGTLRFLGL YRDEHQDFRD EQLRLKKLR GKGKPRKGEG KRAMKKK

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Macromolecule #31: Mitochondrial ribosomal protein S34

MacromoleculeName: Mitochondrial ribosomal protein S34 / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 25.843818 KDa
SequenceString: MARKKVRPRL IAELARRVRA LREQRERPRD SQRYALDYET MMRPHSGNRL PMRAWADVRH ESRLLQLLNR LPLFGLGRLV TRKSWLWQH DEPCYWRLTR VRPDYAAENL DHGKAWGILT FKGKTESEAR EIEQVMYHDW RLVPKHEEEA FTAFTLVPED T PLSVPYPP ...String:
MARKKVRPRL IAELARRVRA LREQRERPRD SQRYALDYET MMRPHSGNRL PMRAWADVRH ESRLLQLLNR LPLFGLGRLV TRKSWLWQH DEPCYWRLTR VRPDYAAENL DHGKAWGILT FKGKTESEAR EIEQVMYHDW RLVPKHEEEA FTAFTLVPED T PLSVPYPP LLRAMILAQR QKYGDTSTEE PMLNLERARI DPWDYPAKLE AKTKTKGAAV

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Macromolecule #32: Mitochondrial ribosomal protein S35

MacromoleculeName: Mitochondrial ribosomal protein S35 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 37.104809 KDa
SequenceString: MAATSLPAWL ILKSRAGTFR AFSTAMSPAT RSPRPALRTT ERTSKPERAL RRKALPPRTE KMAVDQDWPS VYPVAAPFKP SAVPLPVRM GYPVKRGVPM AKEGNLELLK IPNFLHLTPV AIKRHCEALK DFCTEWPAAL DSDEKCEKHF PIEIDTADYV S AGPSIRNP ...String:
MAATSLPAWL ILKSRAGTFR AFSTAMSPAT RSPRPALRTT ERTSKPERAL RRKALPPRTE KMAVDQDWPS VYPVAAPFKP SAVPLPVRM GYPVKRGVPM AKEGNLELLK IPNFLHLTPV AIKRHCEALK DFCTEWPAAL DSDEKCEKHF PIEIDTADYV S AGPSIRNP KARVVTLRVK LSSLNLDDHA KKKLIKLVGD RYCKSTDVLT IKTDRCPLKR QNYDYAVYLL TVLYHESWKT EE WEKKKTE ADMEEYIWEN STSEKNILET LLQIKAAEKN LELSKEELLG TKEVEDYRKS VVSLKNEGDN ENTLSQYKES VKR LLNLA

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Macromolecule #33: Mitochondrial ribosomal protein S37

MacromoleculeName: Mitochondrial ribosomal protein S37 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig)
Molecular weightTheoretical: 13.561903 KDa
SequenceString:
MATPSLRGRL ARLGNPRRPI LKPNKPLILA NHVGERRREK GEATCITEMS VMMACWKQNE FRDEACRKEI QDFFDCASRA EAARKMRSI QENLGELGSL PPKKLNKLLH RFPNKPHVS

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Macromolecule #34: Aurora kinase A interacting protein 1

MacromoleculeName: Aurora kinase A interacting protein 1 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 22.843941 KDa
SequenceString: MFLVRLTSQL LRAVPGAGRS GSPRVSEVLG RHACRPRYST QPPGPSGIAS LPGKHVHPEL EEMLVPRKMS ISPLESWLTI RYLLPRLGA GTPGTVSPAQ LYQCPPSQVG EGVKQGDKEV RDTPQMQCKN ILKIRRRKMN HHKYRKLVKR TRFLRRKVRE G RLRQKQVK ...String:
MFLVRLTSQL LRAVPGAGRS GSPRVSEVLG RHACRPRYST QPPGPSGIAS LPGKHVHPEL EEMLVPRKMS ISPLESWLTI RYLLPRLGA GTPGTVSPAQ LYQCPPSQVG EGVKQGDKEV RDTPQMQCKN ILKIRRRKMN HHKYRKLVKR TRFLRRKVRE G RLRQKQVK FERDLKRIWQ KAGLKEAPAG WQTPKIYLKN Q

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Macromolecule #35: Mitochondrial ribosomal protein S39

MacromoleculeName: Mitochondrial ribosomal protein S39 / type: protein_or_peptide / ID: 35
Details: Unassigned residues have been built as poly-alanine.
Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 76.473766 KDa
SequenceString: MAAVASARWL GVRSGLCLPL TGRRVGPCGR TPRSRFYSGS AAHPEVEGAN VTGIEEVVIP KKKTWDKVAI LQALASTVHR DSTAAPYVF QDDPYLIPTS SVESHSFLLA KKSGENAAKF IINSYPKYFQ KDIAEPHIPC LMPEYFEPQI EDVSEAALQE R IKL(UNK)(UNK) ...String:
MAAVASARWL GVRSGLCLPL TGRRVGPCGR TPRSRFYSGS AAHPEVEGAN VTGIEEVVIP KKKTWDKVAI LQALASTVHR DSTAAPYVF QDDPYLIPTS SVESHSFLLA KKSGENAAKF IINSYPKYFQ KDIAEPHIPC LMPEYFEPQI EDVSEAALQE R IKL(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)TWRTK NNAERIFALM PEKNAHSYC TMIRGMVKHQ APTQALNLYT VLLNNRLRAD VYTFNSLIEA TALVVNEKFE EKWNNILDLL KQMVTQNVKP N LQTFNTIL KCLRRFYAFG KLPALQTLRE MKAIGIEPSL ATYHYVIQLF YQHESPSKGS SLIIYDIMNE VMGKRFSPRD PD DDMFFQS AMRVCSSLRD LELAYQVHGL LNTGDNWKLI GSDHRRNFYY SKFFNLLCFM EQIDVTLKWY KDLIPSVFFP HSQ TMIDLL QALDVANRLD MVPQIWKDSK EYGHTFRNEL KEEILMLMAR DQHPPELQVA FADCAADIKS TYESQDARQT APEW PASSL NYVAVLFLRA GRTQEAWKML GLFRKHNKIP RAELLNEFLD SAKASSSPAQ AIELVKLASA FSLPVCEGLT RRVMA EFTL TQEQREALGE LTALTSDSSS DSDSDSDSDI SEGK

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Macromolecule #36: 28S ribosomal protein S18b, mitochondrial

MacromoleculeName: 28S ribosomal protein S18b, mitochondrial / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Pig (pig) / Organ: liver
Molecular weightTheoretical: 29.220465 KDa
SequenceString: MAASILNVLL RRLPGVSPFR GAYGVQVLLQ TLCTKAPPED DSLPQVPISP YKDEPWKYLD SEEYQNRYGS RPVWADYRRN HKGGIPPQR TRKMCIRGNK VAGNPCPICR DQKLHVDFRN VKLLEQFVCA HTGIIFHAPY TGVCMKQHKK LTQAIQKARD H GLLRYHIP ...String:
MAASILNVLL RRLPGVSPFR GAYGVQVLLQ TLCTKAPPED DSLPQVPISP YKDEPWKYLD SEEYQNRYGS RPVWADYRRN HKGGIPPQR TRKMCIRGNK VAGNPCPICR DQKLHVDFRN VKLLEQFVCA HTGIIFHAPY TGVCMKQHKK LTQAIQKARD H GLLRYHIP QVEPRDLDFS TTHGAVSSTP PAPTLVSGDP WYPWYSWKQP PERELSRLRR LYQGRLREES GPPPELMPEV PL TAPAEAS STEPGAPQSA L

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Macromolecule #3: 12S ribosomal RNA, mitochondrial

MacromoleculeName: 12S ribosomal RNA, mitochondrial / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Sus scrofa (pig) / Organ: liver
Molecular weightTheoretical: 308.989062 KDa
SequenceString: ACACAGGUUU GGUCCUGGCC UUUCUAUUAA UUCUUAAUAA AAUUACACAU GCAAGUAUCC GCGCCCCGGU GAGAAUGCCC UCCAGAUCU UAAAGAUCAA AAGGAGCAGG UAUCAAGCAC ACCUAUAACG GUAGCUCAUA ACGCCUUGCU CAACCACACC C CCACGGGA ...String:
ACACAGGUUU GGUCCUGGCC UUUCUAUUAA UUCUUAAUAA AAUUACACAU GCAAGUAUCC GCGCCCCGGU GAGAAUGCCC UCCAGAUCU UAAAGAUCAA AAGGAGCAGG UAUCAAGCAC ACCUAUAACG GUAGCUCAUA ACGCCUUGCU CAACCACACC C CCACGGGA AACAGCAGUG AUAAAAAUUA AGCCAUGAAC GAAAGUUUGA CUAAGUUAUA UUAAUUAGAG UUGGUAAAUC UC GUGCCAG CCACCGCGGU CAUACGAUUA ACCCAAAUUA AUAGAUCCAC GGCGUAAAGA GUGUUUAAGA AAAAAAAUCA CAA UAGAGU UAAAUUAUAA CUAAGCUGUA AAAAGCCCUA GUUAAAAUAA AAUAACCCAC GAAAGUGACU CUAAUAAUCC UGAC ACACG AUAGCUAGGA CCCAAACUGG GAUUAGAUAC CCCACUAUGC CUAGCCCUAA ACCCAAAUAG UUACAUAACA AAACU AUUC GCCAGAGUAC UACUCGCAAC UGCCUAAAAC UCAAAGGACU UGGCGGUGCU UCACAUCCAC CUAGAGGAGC CUGUUC UAU AAUCGAUAAA CCCCGAUAGA CCUUACCAAC CCUUGCCAAU UCAGCCUAUA UACCGCCAUC UUCAGCAAAC CCUAAAA AG GAACAAUAGU AAGCACAAUC AUAGCACAUA AAAACGUUAG GUCAAGGUGU AGCUUAUGGG UUGGAAAGAA AUGGGCUA C AUUUUCUACA UAAGAAUAUC CACCACACGA AAGUUUUUAU GAAACUAAAA ACCAAAGGAG GAUUUAGCAG UAAAUCAAG AAUAGAGUGC UUGAUUGAAU AAGGCCAUGA AGCACGCACA CACCGCCCGU CACCCUCCUC AAGCAUGUAG UAAUAAAAAU AACCUAUAU UCAAUUACAC AACCAUGCAA GAAGAGACAA GUCGUAACAA GGUAAGCAUA CUGGAAAGUG UGCUUGGAUU A CC

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Macromolecule #19: P-site fMet-tRNAMet, mitochondrial

MacromoleculeName: P-site fMet-tRNAMet, mitochondrial / type: rna / ID: 19 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 22.664498 KDa
SequenceString:
AGUAAGGUCA GCUAAAUAAG CUAUCGGGCC CAUACCCCGA AAAUGUUGGU UAUACCCUUC CCGUACUACC A

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Macromolecule #20: MT-CO3 mRNA, mitochondrial

MacromoleculeName: MT-CO3 mRNA, mitochondrial / type: rna / ID: 20 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 63.946098 KDa
SequenceString: AUGACCCACC AAUCACAUGC CUAUCAUAUA GUAAAACCCA GCCCAUGACC CCUAACAGGG GCCCUCUCAG CCCUCCUAAU GACCUCCGG CCUAGCCAUG UGAUUUCACU UCCACUCCAU AACGCUCCUC AUACUAGGCC UACUAACCAA CACACUAACC A UAUACCAA ...String:
AUGACCCACC AAUCACAUGC CUAUCAUAUA GUAAAACCCA GCCCAUGACC CCUAACAGGG GCCCUCUCAG CCCUCCUAAU GACCUCCGG CCUAGCCAUG UGAUUUCACU UCCACUCCAU AACGCUCCUC AUACUAGGCC UACUAACCAA CACACUAACC A UAUACCAA UGAUGGCGCG AUGUAACACG AGAAAGCACA UAC

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Macromolecule #37: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE

MacromoleculeName: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE / type: ligand / ID: 37 / Number of copies: 1 / Formula: GSP
Molecular weightTheoretical: 539.246 Da
Chemical component information

ChemComp-GSP:
5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE

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Macromolecule #38: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 38 / Number of copies: 111 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #39: SODIUM ION

MacromoleculeName: SODIUM ION / type: ligand / ID: 39 / Number of copies: 1
Molecular weightTheoretical: 22.99 Da

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Macromolecule #40: SPERMINE

MacromoleculeName: SPERMINE / type: ligand / ID: 40 / Number of copies: 1 / Formula: SPM
Molecular weightTheoretical: 202.34 Da
Chemical component information

ChemComp-SPM:
SPERMINE / Spermine

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Macromolecule #41: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 41 / Number of copies: 3 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #42: N-FORMYLMETHIONINE

MacromoleculeName: N-FORMYLMETHIONINE / type: ligand / ID: 42 / Number of copies: 1 / Formula: FME
Molecular weightTheoretical: 177.221 Da
Chemical component information

ChemComp-FME:
N-FORMYLMETHIONINE / N-Formylmethionine

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Macromolecule #43: GUANOSINE-5'-TRIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 43 / Number of copies: 1 / Formula: GTP
Molecular weightTheoretical: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

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Macromolecule #44: water

MacromoleculeName: water / type: ligand / ID: 44 / Number of copies: 5 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER / Water

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.171 mg/mL
BufferpH: 7.6
GridModel: Quantifoil R2/2 / Material: COPPER / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV
Detailscontains 55S mitochondrial ribosome, mitochondrial initiation factor 2, mitochondrial formyl-Met-tRNAMet and MT-CO3 mRNA

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy
Image recordingFilm or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Digitization - Dimensions - Width: 4096 pixel / Digitization - Dimensions - Height: 4096 pixel / Number real images: 13936 / Average exposure time: 1.4 sec. / Average electron dose: 40.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 1366787
CTF correctionSoftware - Name: RELION (ver. 2.1)
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

5aj4

Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 2.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 2.1)
Final reconstructionNumber classes used: 1 / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2.1) / Number images used: 139206

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Atomic model buiding 1

RefinementSpace: RECIPROCAL / Protocol: OTHER / Overall B value: 73.8
Output model

PDB-6gaz:
Unique features of mammalian mitochondrial translation initiation revealed by cryo-EM. This file contains the 28S ribosomal subunit.

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