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- EMDB-4160: Imidazoleglycerol-phosphate dehydratase from Saccharomyces cerevisiae -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-4160 | ||||||||||||
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Title | Imidazoleglycerol-phosphate dehydratase from Saccharomyces cerevisiae | ||||||||||||
![]() | Imidazoleglycerol-phosphate dehydratase | ||||||||||||
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![]() | Inhibitor / histidine biosynthesis / lyase / complex | ||||||||||||
Function / homology | ![]() imidazoleglycerol-phosphate dehydratase / imidazoleglycerol-phosphate dehydratase activity / L-histidine biosynthetic process / metal ion binding Similarity search - Function | ||||||||||||
Biological species | ![]() ![]() | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.2 Å | ||||||||||||
![]() | Rawson S / Bisson C | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Elucidating the structural basis for differing enzyme inhibitor potency by cryo-EM. Authors: Shaun Rawson / Claudine Bisson / Daniel L Hurdiss / Asif Fazal / Martin J McPhillie / Svetlana E Sedelnikova / Patrick J Baker / David W Rice / Stephen P Muench / ![]() Abstract: Histidine biosynthesis is an essential process in plants and microorganisms, making it an attractive target for the development of herbicides and antibacterial agents. Imidazoleglycerol-phosphate ...Histidine biosynthesis is an essential process in plants and microorganisms, making it an attractive target for the development of herbicides and antibacterial agents. Imidazoleglycerol-phosphate dehydratase (IGPD), a key enzyme within this pathway, has been biochemically characterized in both (IGPD) and (IGPD). The plant enzyme, having been the focus of in-depth structural analysis as part of an inhibitor development program, has revealed details about the reaction mechanism of IGPD, whereas the yeast enzyme has proven intractable to crystallography studies. The structure-activity relationship of potent triazole-phosphonate inhibitors of IGPD has been determined in both homologs, revealing that the lead inhibitor (C348) is an order of magnitude more potent against IGPD than IGPD; however, the molecular basis of this difference has not been established. Here we have used single-particle electron microscopy (EM) to study structural differences between the and IGPD homologs, which could influence the difference in inhibitor potency. The resulting EM maps at ∼3 Å are sufficient to de novo build the protein structure and identify the inhibitor binding site, which has been validated against the crystal structure of the IGPD/C348 complex. The structure of _IGPD reveals that a 24-amino acid insertion forms an extended loop region on the enzyme surface that lies adjacent to the active site, forming interactions with the substrate/inhibitor binding loop that may influence inhibitor potency. Overall, this study provides insights into the IGPD family and demonstrates the power of using an EM approach to study inhibitor binding. | ||||||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 14.9 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 12.6 KB 12.6 KB | Display Display | ![]() |
Images | ![]() | 294.8 KB | ||
Filedesc metadata | ![]() | 5.8 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6ezmMC ![]() 3999C ![]() 6ezjC C: citing same article ( M: atomic model generated by this map |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Imidazoleglycerol-phosphate dehydratase | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.065 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Imidazoleglycerol-phosphate dehydratase (IGPD) in complex with tr...
Entire | Name: Imidazoleglycerol-phosphate dehydratase (IGPD) in complex with triazole-phosphonate inhibitor (C384) |
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Components |
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-Supramolecule #1: Imidazoleglycerol-phosphate dehydratase (IGPD) in complex with tr...
Supramolecule | Name: Imidazoleglycerol-phosphate dehydratase (IGPD) in complex with triazole-phosphonate inhibitor (C384) type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1 |
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Source (natural) | Organism: ![]() ![]() |
Molecular weight | Theoretical: 570 KDa |
-Macromolecule #1: Imidazoleglycerol-phosphate dehydratase
Macromolecule | Name: Imidazoleglycerol-phosphate dehydratase / type: protein_or_peptide / ID: 1 / Number of copies: 24 / Enantiomer: LEVO / EC number: imidazoleglycerol-phosphate dehydratase |
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Source (natural) | Organism: ![]() ![]() Strain: ATCC 204508 / S288c |
Molecular weight | Theoretical: 23.867197 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MTEQKALVKR ITNETKIQIA ISLKGGPLAI EHSIFPEKEA EAVAEQATQS QVINVHTGIG FLDHMIHALA KHSGWSLIVE CIGDLHIDD HHTTEDCGIA LGQAFKEALG AVRGVKRFGS GFAPLDEALS RAVVDLSNRP YAVVELGLQR EKVGDLSCEM I PHFLESFA ...String: MTEQKALVKR ITNETKIQIA ISLKGGPLAI EHSIFPEKEA EAVAEQATQS QVINVHTGIG FLDHMIHALA KHSGWSLIVE CIGDLHIDD HHTTEDCGIA LGQAFKEALG AVRGVKRFGS GFAPLDEALS RAVVDLSNRP YAVVELGLQR EKVGDLSCEM I PHFLESFA EASRITLHVD CLRGKNDHHR SESAFKALAV AIREATSPNG TNDVPSTKGV LM UniProtKB: Imidazoleglycerol-phosphate dehydratase |
-Macromolecule #2: MANGANESE (II) ION
Macromolecule | Name: MANGANESE (II) ION / type: ligand / ID: 2 / Number of copies: 48 / Formula: MN |
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Molecular weight | Theoretical: 54.938 Da |
-Macromolecule #3: [(2R)-2-hydroxy-3-(1H-1,2,4-triazol-1-yl)propyl]phosphonic acid
Macromolecule | Name: [(2R)-2-hydroxy-3-(1H-1,2,4-triazol-1-yl)propyl]phosphonic acid type: ligand / ID: 3 / Number of copies: 24 / Formula: 5LD |
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Molecular weight | Theoretical: 207.124 Da |
Chemical component information | ![]() ChemComp-5LD: |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 0.5 mg/mL |
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Buffer | pH: 7.5 |
Grid | Model: Quantifoil R2/2 / Material: COPPER / Pretreatment - Type: GLOW DISCHARGE |
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Number grids imaged: 1 / Number real images: 2827 / Average electron dose: 1.3 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |